Biochemical Engineering Journal最新文献

Harnessing co-expressed L-arabinose and L-ribose isomerases to enhance the biosynthesis of L-ribose 利用共同表达的 L-阿拉伯糖和 L-核糖异构酶提高 L-核糖的生物合成能力

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2024-11-26 DOI: 10.1016/j.bej.2024.109594

Shahid Mahmood , Muhammad Waheed Iqbal , Abdullah Arsalan , Xinrui Tang , Yuvaraj Ravikumar , Mei Zhao , Cunsheng Zhang , Xianghui Qi

L-Ribose is an essential rare sugar used in several industries, including pharmaceutical, food, cosmetics, and agriculture. Recently, the enzymatic production of L-ribose has garnered significant attention due to its considerable advantages over synthetic methods. This study introduces a novel approach for producing L-ribose from L-arabinose. Initially, the genes encoding L-arabinose isomerase (L-AI) from Bacillus subtilis str. 168 (BsL-AI) and L-ribose isomerase (L-RI) from Actinotalea fermentans ATCC 43279 (AfL-RI) were cloned to construct the recombinant plasmid containing the pANY1-BsL-AI/AfL-RI vector and co-expressed in Escherichia coli BL21(DE3). Subsequently, the of co-expression exhibited optimal activity at pH 8.5 and 40 °C in 50 mM Tris-HCl buffer, with 1 mM Mn²⁺ ion. The activity was increased by 33 % and 12 % with Mn²⁺ and Co²⁺ ions, respectively, compared to the control having no metal ions. The scheme comprising 50 g L⁻¹ of co-expressed cells remained comparatively stable up to 60 °C for 2 h. Finally, the co-expression scheme produced 23.52 g L⁻¹ (24 %), 25.87 g L⁻¹ (26 %) and 27.97 g L⁻¹ (28 %) of L-ribose when utilizing L-arabinose concentration of 30, 50 and 100 g L⁻¹, respectively, with 50 g L⁻¹ of co-expressed cells. This study presents a viable methodology for the utilization of L-arabinose to produce L-ribose in slightly alkaline conditions utilizing a co-expression scheme concealing BsL-AI and AfL-RI genes.

L- 核糖是一种重要的稀有糖类，可用于制药、食品、化妆品和农业等多个行业。最近，酶法生产 L-核糖因其相对于合成方法的巨大优势而备受关注。本研究介绍了一种从 L-阿拉伯糖生产 L-核糖的新方法。首先，克隆了枯草芽孢杆菌（Bacillus subtilis str. 168）的 L-阿拉伯糖异构酶（L-AI）（BsL-AI）和放线菌（Actinotalea fermentans ATCC 43279）的 L-核糖异构酶（L-RI）（AfL-RI）的编码基因，构建了含有 pANY1-BsL-AI/AfL-RI 载体的重组质粒，并在大肠杆菌 BL21（DE3）中共同表达。随后，在 pH 值为 8.5、温度为 40 ℃、含有 1 mM Mn2+ 离子的 50 mM Tris-HCl 缓冲液中，共同表达的质粒表现出最佳活性。与不含金属离子的对照组相比，含 Mn2+ 和 Co2+ 离子的活性分别提高了 33% 和 12%。由 50 g L-1 的共表达细胞组成的方案在 60 °C 下 2 小时内保持相对稳定。最后，当利用 L-阿拉伯糖浓度分别为 30、50 和 100 g L-1 时，共表达方案与 50 g L-1 的共表达细胞分别产生了 23.52 g L-1 （24%）、25.87 g L-1 （26%）和 27.97 g L-1 （28%）的 L-核糖。本研究提出了一种可行的方法，利用隐藏 BsL-AI 和 AfL-RI 基因的共表达方案，在微碱性条件下利用 L-阿拉伯糖生产 L-核糖。

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引用次数: 0

in vitro analysis of a competitive inhibition model for T7 RNA polymerase biosensors 体外分析 T7 RNA 聚合酶生物传感器的竞争性抑制模型

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2024-11-23 DOI: 10.1016/j.bej.2024.109590

Ryan M. Delaney, Katherine A. Lamb, Olivia M. Irvin, Zachary T. Baumer, Timothy A. Whitehead

T7 RNA polymerase (T7 RNAP) biosensors, in which T7 RNAP transcribes some reporter gene or signal in response to external stimuli, have wide applications in synthetic biology and metabolic engineering. We adapted a biochemical reaction network model and used an in vitro transcription assay to determine network parameters for different T7 RNAP constructs. Under conditions where template DNA is limiting, the EC₅₀ values of native and engineered T7 RNAPs ranged from 33 nM (29–37 95 % c.i.) to 570 nM (258–714 95 % c.i.) (wild-type T7 RNAP). The measured EC₅₀ values were largely insensitive to free magnesium, pH, or other buffer conditions. Many biosensor configurations use a split RNAP construct, where the C-terminal (CT7) and N-terminal T7 (NT7) are fused to proximity induced dimerization modules. We used proteolysis and ion exchange chromatography to prepare a CT7 (80 kDa) product. The impact of free CT7 on T7 RNAP transcriptional activity was well described by a competitive inhibition model, with an inhibitory constant K_I = 23 nM (18–28 95 % c.i.) of the sensor. These model parameters will be useful for forward modeling and design of T7 RNAP-based genetic circuits.

T7 RNA 聚合酶（T7 RNAP）生物传感器是指 T7 RNAP 在外界刺激下转录一些报告基因或信号，它在合成生物学和代谢工程中有着广泛的应用。我们改编了一个生化反应网络模型，并利用体外转录试验确定了不同 T7 RNAP 构建物的网络参数。在限制模板 DNA 的条件下，原生和工程化 T7 RNAP 的 EC50 值从 33 nM（29-37 95 % c.i.）到 570 nM（258-714 95 % c.i.）（野生型 T7 RNAP）不等。测得的 EC50 值对游离镁、pH 值或其他缓冲条件基本不敏感。许多生物传感器配置使用的是分裂的 RNAP 构建物，其中 C 端 (CT7) 和 N 端 T7 (NT7) 与近距离诱导的二聚化模块融合。我们使用蛋白水解和离子交换色谱法制备了 CT7（80 kDa）产物。游离 CT7 对 T7 RNAP 转录活性的影响通过竞争抑制模型得到了很好的描述，传感器的抑制常数 KI = 23 nM（18-28 95 % c.i.）。这些模型参数将有助于基于 T7 RNAP 的基因回路的前瞻性建模和设计。

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引用次数: 0

A data-driven approach for cell culture medium optimization 数据驱动的细胞培养基优化方法

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2024-11-23 DOI: 10.1016/j.bej.2024.109591

Yuki Ozawa, Takamasa Hashizume, Bei-Wen Ying

Cell culture media are critical for cell propagation and bioproduction to be as efficient as possible to meet medical and pharmaceutical requirements. However, optimizing medium composition to achieve optimal cell culture remains a significant challenge due to the complexity of living cells and their required media. This study, which is a significant contribution to the field, addresses the need for data-driven techniques in cell culture technologies by integrating active machine learning (ML) to reformulate a widely used base medium for mammalian cell culture. The optimization process was facilitated by developing various ML models, which accounted for experimental data processing and time consumption. It provided a detailed methodology and essential knowledge for utilizing ML in medium development. Growth determinative medium components were identified through data mining and scale-up culture. In addition, RNA sequencing analysis indicated that active learning finetuned the media for the changes in gene expression for improved cell culture. This study offers new insights and methodologies to be applied to cell culture for future medical purposes.

细胞培养基对于尽可能高效地进行细胞繁殖和生物生产以满足医疗和制药要求至关重要。然而，由于活细胞及其所需培养基的复杂性，优化培养基成分以实现最佳细胞培养仍是一项重大挑战。本研究通过整合主动机器学习（ML）来重新配置一种广泛应用于哺乳动物细胞培养的基础培养基，满足了细胞培养技术中对数据驱动技术的需求，是对该领域的重大贡献。通过开发各种 ML 模型，考虑到实验数据处理和时间消耗，促进了优化过程。它为在培养基开发中利用 ML 提供了详细的方法和基本知识。通过数据挖掘和放大培养，确定了决定生长的培养基成分。此外，RNA 测序分析表明，主动学习可根据基因表达的变化对培养基进行微调，从而改进细胞培养。这项研究提供了新的见解和方法，可应用于未来医学目的的细胞培养。

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引用次数: 0

Engineering central metabolism in Yarrowia lipolytica increases lipid accumulation 脂肪溶解亚罗菌的中枢代谢工程可增加脂质积累

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2024-11-22 DOI: 10.1016/j.bej.2024.109589

Tadej Markuš , Mladen Soldat , Vasilka Magdevska , Jaka Horvat , Martin Kavšček , Gregor Kosec , Štefan Fujs , Uroš Petrovič

Yarrowia lipolytica, a non-conventional oleaginous yeast, holds great promise for a wide range of metabolic engineering applications. Strains of this species are renowned for their capacity to accommodate high carbon flux through acetyl-CoA and are, therefore, being extensively studied for producing chemicals derived from this precursor molecule. We investigated the effects of the pyruvate bypass, phosphoketolase bypass, and the carnitine shuttle on Y. lipolytica’s central carbon metabolism by monitoring the production of lipids and β-carotene. We constructed a set of parental strains derived from the Y. lipolytica YB-392 wild-type strain, introduced the metabolic bypasses, and subjected the constructed strains to a series of fermentation experiments. We discovered that upon introducing the modifications to central carbon metabolism, carbon is preferentially directed through the lipid biosynthesis pathway, making such engineering an effective strategy for increasing lipid production. The highest lipid content of 0.61 g_lipids/g_DCW was recorded after introducing the carnitine shuttle-associated gene YlCAT2. Meanwhile, the highest increase in production efficiency, 78.2 %, was recorded for a combination of genes associated with the pyruvate bypass (YlPDC1, YlALD3, YlACS1).

脂溶性亚罗酵母（Yarrowia lipolytica）是一种非常规的含油酵母，在广泛的代谢工程应用中大有可为。该菌株以其通过乙酰-CoA 来适应高碳通量的能力而闻名，因此被广泛研究用于生产由这种前体分子衍生的化学物质。我们通过监测脂质和β-胡萝卜素的生产，研究了丙酮酸旁路、磷酸酮酶旁路和肉碱穿梭对溶脂酵母的中心碳代谢的影响。我们构建了一组源自脂溶性酵母 YB-392 野生型菌株的亲本菌株，引入了代谢旁路，并对所构建的菌株进行了一系列发酵实验。我们发现，在引入对中心碳代谢的改造后，碳会优先通过脂质生物合成途径，从而使这种工程改造成为提高脂质产量的有效策略。引入肉碱穿梭相关基因 YlCAT2 后，脂质含量最高，达到 0.61g/gDCW。同时，与丙酮酸旁路相关的基因组合（YlPDC1、YlALD3、YlACS1）的生产效率增幅最高，达到 78.2%。

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引用次数: 0

Research progress on solid-phase electron donors for the denitrification of wastewater: A review 用于废水脱氮的固相电子供体的研究进展：综述

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2024-11-22 DOI: 10.1016/j.bej.2024.109575

Baoshan Wang , Xingxiang Zhang , Zhilong Dong , Xiaojie Chen , Chengcheng Wen , Zhiyuan Wang , Yingming Liu , Enli Liu

Electron donor-mediated biological denitrification, the most extensively employed method for the treatment of nitrate nitrogen (NO₃⁻-N), involves NO₃⁻-N directly or indirectly by acquiring electrons provided by electron donors and converting them into N₂. Currently, the most widely researched electron donors are gaseous, liquid, and solid forms. Owing to the difficulties in storing and transporting gaseous and liquid electron donors, and their potential for causing secondary pollution, solid-phase electron donors (SPEDs), which can be slowly utilized by microorganisms, have gradually gained attention. SPEDs not only serve as a carrier for microbial attachment, but most SPEDs are also low-cost and readily available, making them advantageous for practical applications. In this review, the different types of SPEDs are classified and their microbial utilization mechanisms in the biological denitrification process discussed based on their classification. Their denitrification performance, influencing factors, practical applications, and existing issues are summarized. This review provides a reference for future research on SPED and its applications. It also provides an outlook on SPED-mediated mixotrophic denitrification and SPED-coupled electrochemical technology for enhanced nitrogen removal processes, in view of this hot direction in SPED research.

电子供体介导的生物脱硝是处理硝态氮（NO3--N）最广泛采用的方法，NO3--N 直接或间接地通过获取电子供体提供的电子并将其转化为 N2。目前，研究最广泛的电子供体有气态、液态和固态。由于气态和液态电子供体难以储存和运输，且可能造成二次污染，可被微生物缓慢利用的固态电子供体（SPED）逐渐受到关注。固相电子供体不仅可以作为微生物附着的载体，而且大多数固相电子供体成本低廉、易于获得，因此在实际应用中具有很大的优势。本综述对不同类型的 SPED 进行了分类，并根据分类讨论了它们在生物脱氮过程中的微生物利用机制。总结了它们的脱硝性能、影响因素、实际应用和存在的问题。本综述为今后有关 SPED 及其应用的研究提供了参考。鉴于 SPED 研究的这一热点方向，本综述还对 SPED 介导的混养反硝化和 SPED 耦合电化学技术用于增强脱氮过程进行了展望。

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引用次数: 0

Particles made of a novel recombinant spider silk protein rAcSp2 as delivery system for peptide drugs with anti-tumor activity 用新型重组蜘蛛丝蛋白 rAcSp2 制成的颗粒作为具有抗肿瘤活性的多肽药物的递送系统

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2024-11-21 DOI: 10.1016/j.bej.2024.109588

Fengman Han , Xianglong Li , Yu Tan , Qingyu Zhou

The recombinant spider silk proteins (spidroins) are promising biomaterials for the use as drug delivery system (DDS) because of their non-cytotoxicity, low immunogenicity and customizable properties. However, most reported spidroin-based materials as DDS derive from the repetitive domain of dragline silk protein, limiting us to take advantage of their desirable properties for medical and industrial innovation. Here, we produced the recombinant aciniform silk protein (rAcSp2) that contains only the N-terminal domain and formulated it into nanoparticles for use as a DDS. We demonstrated that antitumor peptide drugs such as ChMAP-28 can be loaded onto rAcSp2 particles via electrostatic interaction, with a high loading capacity of up to 45 % (w/w) and nearly 100 % loading efficiency. In addition, the release of ChMAP-28 depends on the pH and ionic strength of the release buffer. In the meantime, rAcSp2 particles not only effectively reduce the toxicity of ChMAP-28 to normal cells, but also significantly enhance its anti-tumor activity. Therefore, our rAcSp2 particles are a promising novel particulate drug carrier system for the delivery of peptide drugs with anti-tumor activity.

重组蛛丝蛋白（spidroins）具有无细胞毒性、低免疫原性和可定制的特性，是一种很有希望用作药物输送系统（DDS）的生物材料。然而，大多数已报道的基于螺旋藻蛋白的 DDS 材料都来自龙蚕丝蛋白的重复结构域，这限制了我们利用其理想特性进行医疗和工业创新。在这里，我们制备了仅含有 N 端结构域的重组刺丝蛋白（rAcSp2），并将其配制成纳米颗粒用作 DDS。我们证明了 ChMAP-28 等抗肿瘤多肽药物可通过静电作用负载到 rAcSp2 颗粒上，负载能力高达 45%（重量比），负载效率接近 100%。此外，ChMAP-28 的释放取决于释放缓冲液的 pH 值和离子强度。同时，rAcSp2 颗粒不仅能有效降低 ChMAP-28 对正常细胞的毒性，还能显著增强其抗肿瘤活性。因此，我们的 rAcSp2 颗粒是一种很有前景的新型颗粒载药系统，可用于递送具有抗肿瘤活性的多肽药物。

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引用次数: 0

Recent advances in bio-based production of organic acids by genetically engineered yeasts

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2024-11-21 DOI: 10.1016/j.bej.2024.109587

Ning Xu , Haiyan Gao , Yanran Wang , Chenxuan Liu , Lei Hu , Aiyong He , Wankui Jiang , Fengxue Xin

In order to achieve the sustainable development of society and reduce environmental footprint of chemical industry, some cleaner and greener production methods are still needed. Microbial fermentation has shown great potentials for sustainable production of chemicals, which can convert renewable resources into different products under mild conditions. Particularly, organic acids production has become a highly competitive and rapidly developed field, as they show broad applications in food, cosmetic and medical areas. Especially, yeasts have shown many advantages in organic acids production especially at high osmotic pressure or low pH. Accordingly, this review will discuss the latest progress of engineered yeasts as cell factories for organic acids production. Different strategies for improvement of organic acids production will be introduced, and future perspectives will also be proposed.

引用次数: 0

Study on activation and performance of recycled hardened concrete powder based on microbial accelerated carbonization 基于微生物加速碳化的再生硬化混凝土粉末活化和性能研究

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2024-11-20 DOI: 10.1016/j.bej.2024.109586

X. Li , XY Wang , M. Li , QW Zhan , WY Dong

Recycled hardened concrete powder (RHCP) was used as a raw material, and microorganisms were introduced to accelerate carbonization to enhance its activity. The carbonation ability of RHCP was investigated by altering the solid-liquid ratio during carbonation. The results indicated that the carbon sequestration amount of RHCP after 7 days of carbonation at room temperature and pressure initially increased and then decreased with the rise of the solid-liquid ratio. When the solid-liquid ratio was 1:0.8, the carbon sequestration amount of RHCP was the highest, reaching 208.65 g/kg. The carbonized RHCP (C-RHCP) and RHCP were then used to partially replace cement to investigate their effects on the workability, mechanical, and durability properties of the mixtures. The results showed that when the replacement amount of C-RHCP was 30 %, all the properties were relatively good. The water demand and setting time were not significantly different. After 28 days of curing, the compressive strength of the 30 % C-RHCP specimen reached over 70 % of that of the pure cement group. However, its carbonation resistance was significantly lower than that of pure cement, while its resistance to chloride ion penetration and drying shrinkage was similar.

以再生硬化混凝土粉（RHCP）为原料，引入微生物加速碳化以提高其活性。通过改变碳化过程中的固液比，研究了 RHCP 的碳化能力。结果表明，在常温常压下碳化 7 天后，RHCP 的固碳量随着固液比的升高先增加后减少。当固液比为 1:0.8 时，RHCP 的固碳量最高，达到 208.65 克/千克。然后用碳化 RHCP（C-RHCP）和 RHCP 部分替代水泥，研究它们对混合物工作性、机械和耐久性能的影响。结果表明，当 C-RHCP 的替代量为 30% 时，所有性能都相对较好。需水量和凝结时间没有明显差异。养护 28 天后，30% C-RHCP 试样的抗压强度达到纯水泥组的 70%以上。不过，其抗碳化性能明显低于纯水泥，而抗氯离子渗透和干燥收缩性能则与纯水泥相似。

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引用次数: 0

Temperature tactics: Targeting acetate or methane production in autotrophic H2/CO2 conversion with mixed cultures 温度战术：利用混合培养物在自养型 H2/CO2 转化过程中瞄准醋酸盐或甲烷的生产

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2024-11-19 DOI: 10.1016/j.bej.2024.109574

Paola A. Palacios , Mads U. Sieborg , Simon B. Kuipers , Simon Fruergaard , Michael V.W. Kofoed

Ex situ biomethanation is a promising technology that combines the concepts of Power-to-X and carbon capture and utilization (CCU) by employing microorganisms to convert hydrogen (H₂) and carbon dioxide (CO₂) into biomethane (CH₄). Mesophilic (37°C) and thermophilic (50°C) ex situ biomethanation processes in continuous stirred tank reactors (CSTRs), were evaluated for the first time under a high continuous H₂/CO₂ supply. Each process was evaluated with and without the addition of minerals and vitamins. In all thermophilic reactors, methanogenesis was favored, achieving stable methane yields of 110 ± 8 %. Additionally, the addition of minerals resulted in a 67 % increase in H₂ consumption rates. In contrast, mesophilic reactors showed low CH₄ yields (7.5 %) and acetogenesis as the dominant pathway, with acetate concentrations reaching up to 7.6 ± 2 g L⁻¹. The effect of free acetic acid (FAA), a rarely considered concept based on the correlation between pH and acetic acid concentration, was also tested on thermophilic ex situ CSTRs. A clear negative correlation was demonstrated between FAA concentration and the CH₄ productivity, with a 50 % inhibition of CH₄ productivity at 0.062 g L⁻¹ of FAA.

原位生物甲烷化是一种前景广阔的技术，它结合了电能转换和碳捕集与利用（CCU）的概念，利用微生物将氢（H2）和二氧化碳（CO2）转化为生物甲烷（CH4）。在连续搅拌罐反应器（CSTR）中的嗜中（37°C）和嗜热（50°C）原位生物甲烷化工艺首次在连续高浓度 H2/CO2 供应条件下进行了评估。每种工艺都在添加和不添加矿物质和维生素的情况下进行了评估。在所有嗜热反应器中，甲烷生成都很有利，甲烷产量稳定在 110 ± 8 %。此外，添加矿物质使 H2 消耗率增加了 67%。相比之下，中嗜酸反应器的 CH4 产率较低（7.5%），乙酸生成是主要途径，乙酸浓度高达 7.6 ± 2 g L-1。游离乙酸（FAA）是一个很少被考虑的概念，它基于 pH 值和乙酸浓度之间的相关性，也在嗜热原位反应器中进行了测试。结果表明，游离乙酸浓度与 CH4 产率之间存在明显的负相关关系，游离乙酸浓度为 0.062 g L-1 时，CH4 产率的抑制率为 50%。

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引用次数: 0

CFD predictive simulations of miniature bioreactor mixing dynamics coupled with photo-bioreaction kinetics in transitional flow regime 微型生物反应器混合动力学与过渡流态光生物反应动力学的 CFD 预测模拟

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2024-11-19 DOI: 10.1016/j.bej.2024.109585

Bovinille Anye Cho , George Mbella Teke , Godfrey K. Gakingo , Robert William McClelland Pott , Dongda Zhang

High-throughput systems using miniaturised stirred bioreactors accelerate bioprocess development due to their simplicity and low cost. However, fluctuating hydrodynamics pose numerical challenges for coupling (bio)reaction kinetics, critical for optimisation and scale-up/down in chemical and bioprocess industries. To address this, hydrodynamic convergence was achieved by time-averaging instantaneous RANS solutions of the transitional SST model over a sufficiently long period to achieve statistical significance in step one. Subsequently, photo-bioreaction transport models, accounting for the photobioreactor’s directional illumination and curvature, were solved based on these converged fields, overcoming two-step coupling challenges in an approach not previously reported. Applied to a 0.7 L Schott bottle photobioreactor mechanically mixed by a magnetic stirrer (100–500 rpm), the model accurately predicted swirly vortex fields at 500 rpm, with a 7 % error margin for simulated tracer diffusion, and aligned biomass growth profiles with literature data on Rhodopseudomonas palustris. However, parallel computing efficiency did not scale linearly with processor count, making time-averaging computationally expensive. Also, improved bioreactor mixing enhanced biomass productivity, but rpms over 300 required increased incident light intensity (>100 Wm⁻²) due to observed light limitation. Hence, this model facilitates optimising stirring speeds and refining operational parameters for scale-up and scale-down processes.

使用微型搅拌生物反应器的高通量系统因其简单和低成本而加速了生物工艺的开发。然而，波动的流体力学给耦合（生物）反应动力学带来了数值挑战，而耦合（生物）反应动力学对于化学和生物工艺行业的优化和放大/缩小至关重要。为了解决这个问题，我们通过对过渡 SST 模型的瞬时 RANS 解进行足够长的时间平均来实现流体力学收敛，从而在第一步中达到统计意义。随后，根据这些收敛场求解了光生物反应器的定向光照和曲率的光生物反应传输模型，以一种以前从未报道过的方法克服了两步耦合的挑战。将该模型应用于通过磁力搅拌器（100-500 rpm）进行机械混合的 0.7 L 肖特瓶光生物反应器中，该模型准确预测了 500 rpm 转速下的漩涡场，模拟示踪剂扩散的误差率为 7%，生物量生长曲线与有关古朴红单胞菌的文献数据一致。然而，并行计算效率并没有随着处理器数量的增加而线性增加，因此时间平均计算的成本很高。此外，改进生物反应器混合可提高生物量生产率，但由于观察到的光照限制，转速超过 300 时需要增加入射光强度（100 Wm-2）。因此，该模型有助于优化搅拌速度，完善放大和缩小工艺的操作参数。

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引用次数: 0