Biochemical Engineering Journal最新文献

Simultaneous removal of nitrate, zinc, and bisphenol A by an iron-modified biochar composite mycelial pellet bioreactor: Optimization and microbial mechanisms 铁修饰生物炭复合菌丝球生物反应器同时去除硝酸盐、锌和双酚A：优化和微生物机制

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-07-01 Epub Date: 2026-03-10 DOI: 10.1016/j.bej.2026.110161

Yinan Wang , Jiayao Ma , Junfeng Su , Yu Liu , Li Luo , Yihan Bai , Xuan Li

In this study, iron-modified biochar composite mycelial pellets (CMPs) were utilized to immobilize the Fe^2 + oxidizing denitrifying bacterial strain Zoogloea sp. FY-6, with the aim of simultaneous removal of nitrate (NO₃^--N), zinc (Zn²⁺) and bisphenol A (BPA). Under conditions of 8 h hydraulic retention time (HRT), pH 7.0, and influent concentrations of 25.0 mg L⁻¹ NO₃^--N, 1.0 mg L⁻¹ Zn²⁺, and 1.0 mg L⁻¹ BPA, the bioreactor reached its best performance, with NO₃^--N, Zn²⁺, and BPA removal efficiencies of 87.2%, 92.4%, and 94.4%, respectively. The experimental results demonstrated that the addition of iron-modified biochar can enhance denitrification. The removal of Zn²⁺ and BPA was primarily attributed to adsorption and coprecipitation mediated by biogenic precipitates formed during bioreactor operation. Through high-throughput sequencing and metabolic pathway analysis, it was found that Bacteroidia, Alphaproteobacteria, and Clostridia were key contributors to denitrification and contaminant degradation. Metabolic pathway analysis further elucidated the synergistic relationships between these microbial communities and the iron-modified biochar. This research provides new insights into the treatment of complex industrial wastewater using multifunctional microbial systems.

本研究利用铁修饰生物炭复合菌丝球（CMPs）固定化Fe2 +氧化反硝化菌株zogloea sp. FY-6，同时去除硝酸盐（NO3——N）、锌（Zn2+）和双酚A （BPA）。在水力停留时间（HRT）为8 h、pH为7.0、进水浓度为25.0 mg L−1 NO3——N、1.0 mg L−1 Zn2+和1.0 mg L−1 BPA的条件下，生物反应器达到最佳效果，NO3——N、Zn2+和BPA的去除率分别为87.2%、92.4%和94.4%。实验结果表明，添加铁改性生物炭可以增强脱氮效果。Zn2+和BPA的去除主要是由于生物反应器运行过程中形成的生物沉淀介导的吸附和共沉淀。通过高通量测序和代谢途径分析发现，Bacteroidia、Alphaproteobacteria和Clostridia是反硝化和污染物降解的关键贡献者。代谢途径分析进一步阐明了这些微生物群落与铁修饰生物炭之间的协同关系。本研究为利用多功能微生物系统处理复杂工业废水提供了新的见解。

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引用次数: 0

Microgravity mutagenesis in E. coli: A molecular mechanism for high-yield cadaverine production 大肠杆菌微重力诱变：一种高产尸胺的分子机制

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-07-01 Epub Date: 2026-03-02 DOI: 10.1016/j.bej.2026.110147

Guangqiang Shui , Beiya Zhou , Jun Ma , Disen Zhang , Ying Bi , Yuhong Huang

Cadaverine serves as a monomer with significant potential in the industrial synthesis of polyamides, particularly nylon 5X. However, its broad application remains constrained by low microbial productivity and inherent cellular toxicity. Currently, the molecular mechanisms governing cadaverine production and tolerance in E. coli remain incompletely elucidated. In this study, we developed two engineered strains, ΔE. coli LdcEt-D8 and ΔE. coli LdcEt-MG-I6, through microgravity mutagenesis and adaptive laboratory evolution. These strains exhibited remarkable performance enhancements: cadaverine concentration (g L⁻¹) in the whole-cell catalytic reaction increased by 191% and 412%, respectively, while cadaverine tolerance rose by 139% and 193%, relative to the parental strain E. coli LdcEt. Whole genome and transcriptomic analyses revealed that enhanced central carbon metabolism pathway contributed to increased cadaverine production. Concurrent upregulation of amino acid metabolism pathway, fatty acid synthesis pathway, and genetic information repair pathway correlated strongly with improved cadaverine tolerance. Validation experiments on mutant genes confirmed that individual overexpression of purA, accC, holB, cysM, and prps in E. coli BL21(DE3) consistently enhanced cadaverine production, underscoring the indispensable role of mutant genes in the biosynthesis pathway. Collectively, these findings provide insight into the molecular mechanism behind the improved production in mutant strains, as well as decoding the transcriptomic landscape, which provides key targets for advancing whole-cell catalytic synthesis of cadaverine in E. coli.

尸胺作为一种单体，在聚酰胺的工业合成中具有重要的潜力，特别是尼龙5X。然而，它的广泛应用仍然受到低微生物生产力和固有的细胞毒性的限制。目前，控制尸体碱在大肠杆菌中产生和耐受的分子机制尚未完全阐明。在这项研究中，我们开发了两个工程菌株ΔE。大肠杆菌LdcEt-D8和ΔE。大肠杆菌LdcEt-MG-I6，通过微重力诱变和适应性实验室进化。这些菌株在全细胞催化反应中的尸胺浓度（g L−1）比亲本菌株LdcEt分别提高了191%和412%，尸胺耐受性分别提高了139%和193%。全基因组和转录组学分析表明，中心碳代谢途径的增强有助于增加尸胺的产量。氨基酸代谢途径、脂肪酸合成途径和遗传信息修复途径的同步上调与尸胺耐受性的提高密切相关。突变基因的验证实验证实，在大肠杆菌BL21（DE3）中，个体过表达purA、accC、holB、cysM和prps持续提高尸胺的产量，强调突变基因在生物合成途径中不可或缺的作用。总的来说，这些发现提供了对突变菌株提高产量背后的分子机制的深入了解，以及解码转录组景观，这为推进大肠杆菌全细胞催化合成尸胺提供了关键靶点。

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引用次数: 0

Methodological framework for fused filament fabrication of 3D-printed beads for cell immobilization in bioprocesses 用于生物过程中细胞固定化的3d打印珠的熔丝制造方法框架

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-07-01 Epub Date: 2026-03-05 DOI: 10.1016/j.bej.2026.110146

Ricardo Gonzalo Ramírez Brenes , Rubén Ruiz Simón , Isabella Maria Tenório Soares Santos , Victoria E. Santos Mazorra , Ninoska Bojorge Ramírez , Nei Pereira Jr

This study presents an innovative methodology for fabricating polymeric beads via 3D printing to enhance cell immobilization strategies in bioprocess engineering. Using fused filament fabrication (FFF), beads composed of acrylonitrile butadiene styrene (ABS) and polylactic acid (PLA) were fabricated with precisely tailored geometries, enabling the systematic evaluation of how material type and internal design influence both manufacturing feasibility and structural stability. The methodology demonstrates high reproducibility, dimensional accuracy and flexibility, allowing researchers to fabricate immobilization beads adapted to specific bioprocess conditions. In contrast to conventional immobilization matrices, this FFF-based approach offers a scalable, cost-effective and customizable alternative, capable of producing complex porous architectures that promote microbial adhesion and mass transfer. The method stands out for extending additive manufacturing applications beyond enzyme immobilization toward whole-cell biocatalyst systems, providing a valuable framework for future biotechnological process development.

本研究提出了一种通过3D打印制造聚合物珠的创新方法，以增强生物工艺工程中的细胞固定化策略。采用熔丝制造技术（FFF），由丙烯腈-丁二烯-苯乙烯（ABS）和聚乳酸（PLA）组成的微珠以精确定制的几何形状制造出来，从而能够系统地评估材料类型和内部设计如何影响制造可行性和结构稳定性。该方法具有高重复性、尺寸精度和灵活性，使研究人员能够制造适应特定生物工艺条件的固定珠。与传统的固定基质相比，这种基于fff的方法提供了一种可扩展、经济高效和可定制的替代方案，能够产生复杂的多孔结构，促进微生物的粘附和传质。该方法将增材制造的应用范围从酶固定化扩展到全细胞生物催化剂系统，为未来生物技术过程的发展提供了一个有价值的框架。

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引用次数: 0

Production of high-valued D-allulose from sucrose via in vitro multi-enzyme cascade 体外多酶级联酶法从蔗糖制备高价值D-allulose

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-07-01 Epub Date: 2026-03-08 DOI: 10.1016/j.bej.2026.110158

Tian Xiao , Wei Zhao , Ruijin Yang , Xiaomei Lyu

D-allulose is a prominent functional rare sugar with extensive applications in the food, cosmetic, and pharmaceutical fields. However, its commercial production approach via D-allulose 3-epimerase suffers from low conversion yield. In this study, a novel in vitro multi-enzyme cascade pathway, consisting of hydrolysis, isomerization, phosphorylation, and dephosphorylation, was constructed for high conversion of sucrose to D-allulose. By optimizing the reaction conditions and catalytic pathways, we achieved a D-allulose yield of 70.2% from 10 g/L sucrose. To investigate its potential in industrial catalysis, the sucrose concentration was increased to 50 g/L and resulted in a yield of 66.44% D-allulose. This study provides an efficient and cost-effective approach for producing D-allulose from sucrose.

D-allulose是一种重要的功能性稀有糖，在食品、化妆品和制药等领域有着广泛的应用。然而，通过D-allulose 3- epimase进行商业化生产的方法存在转化率低的问题。本研究构建了一种由水解、异构化、磷酸化和去磷酸化组成的体外多酶级联途径，以实现蔗糖向D-allulose的高转化。通过优化反应条件和催化途径，以10 g/L蔗糖为原料，D-allulose的产率达到70.2%。为考察其工业催化潜力，将蔗糖浓度提高到50 g/L， D-allulose的产率为66.44%。本研究为从蔗糖中提取D-allulose提供了一种高效、经济的方法。

引用次数: 0

Hydrodynamic optimization of a newly designed and fabricated U-Loop bioreactor using Taguchi–ANOVA analysis 新设计和制造的U-Loop生物反应器流体动力学优化的田口方差分析

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-07-01 Epub Date: 2026-02-23 DOI: 10.1016/j.bej.2026.110141

Sohrab Valadbeigy , Mohammad Naghashzadegan , Reza H. Sajedi , Asma Chegeni

This study focuses on the design and hydrodynamic optimization of a newly engineered U-loop bioreactor aimed to improve mixing performance in viscous media for Single-Cell Protein (SCP) production and also compared the Statistical and Practical Optimum which considers industrial condition. Three major innovations were implemented: first, the reduction of pump power to a practical optimum value of 8600 W/m³ and improve mixing efficiency; second, the proposal of optimal operational parameters, including the practical optimum culture medium viscosity of 0.045 Pa.s (0.2-wt% CMC), as an indicator of high SCP concentrations, the optimal inoculum (400 g/Lit), and the optimal static-mixer position ( close to degassing tank inlet); and third, the design and testing of a custom swirl static mixer to enhance circulation and mixing. The effects of these parameters were evaluated using a Taguchi L9 experimental design and ANOVA. The results showed that medium viscosity, was the dominant factor affecting mixing time, followed by pump power and static-mixer position. The experimentally validated statistical optimum configuration of 0-wt% CMC, 96 W pump power, 45-cm static-mixer placement, and 400 g/Lit inoculum results in an average mixing time of 9.9 s, in close agreement with the Taguchi prediction. This study provides a mechanistic and statistically grounded framework for optimizing U-loop bioreactors, offering a solid foundation for SCP production.

为提高单细胞蛋白（SCP）生产中黏性介质的混合性能，对新型u型环型生物反应器进行了设计和流体动力学优化，并比较了考虑工业条件的统计优化和实际优化。实施了三个主要创新：首先，将泵功率降低到8600 W/m3的实用最佳值，提高了混合效率；其次，提出了最佳操作参数，其中实际最佳培养基粘度为0.045 Pa。s (0.2 wt% CMC)，作为高SCP浓度的指标，最佳接种量（400 g/Lit）和最佳静态混合器位置（靠近脱气罐入口）；第三，设计和测试了一种定制的涡流静态混合器，以增强循环和混合。采用田口L9试验设计和方差分析评估这些参数的影响。结果表明，介质粘度是影响搅拌时间的主要因素，其次是泵功率和静态混合器位置。实验验证了0-wt% CMC、96 W泵功率、45 cm静态混合器位置和400 g/Lit接种量的统计最佳配置，平均混合时间为9.9 s，与田口的预测非常吻合。本研究为优化u型环生物反应器提供了一个机制和统计基础框架，为SCP生产提供了坚实的基础。

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引用次数: 0

Ferric pyrophosphate-activated N and S self-doped keratin biochar derived from rabbit hair enables efficient Cr(VI) removal: Synergistic effect of Fe(Ⅱ) with N and S 从兔毛中提取的焦磷酸铁活化的N和S自掺杂角蛋白生物炭能够高效去除Cr(VI): Fe（Ⅱ）与N和S的协同效应

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-07-01 Epub Date: 2026-03-09 DOI: 10.1016/j.bej.2026.110160

Zhenjie Chen , Huan Yang , Zelun Su , Xiaoqing Wang , Xiangyun Chen , Na Pang

The removal of Cr(VI) from wastewater by biochar is limited by the surface chemical structure. The doping structure is the key for biochar to improve the surface chemical properties. In this study, the N,S,Fe self-doped biochar for Cr(VI) removal was prepared by pyrolysis and activation by Fe₄(P₂O₇)₃ of waste rabbit hair. The results show that Fe-RBC successfully retains the oxygen-containing and N functional groups in keratin, simultaneously generating

{SO}_{4}^{2 -}

and

S^{2 -}

, and introducing Fe^2 + . The findings of Cr(VI) removal study indicated that the 1.2 g/L biochar exhibited a removal efficiency of approximately 98.33% for Cr(VI) in wastewater within 6 h. The XPS and CV results show that the carbon skeleton constitutes a stable conductive and adsorption substrate. S participates in and promotes the cycling of Fe (II) and Fe (III), and then serves as a direct electron transfer medium to reduce Cr(VI). The significantly enhanced aromatic π electron cloud obtained by graphite-n contributing electrons to the carbon skeleton π system provides adsorption and fixation sites for the reduction product Cr(III) through cation -π interactions. This research fully exploited the natural structural advantages of protein fibers and achieved in-situ controllable preparation of heteroatom self-doped biochar.

生物炭对废水中Cr（VI）的去除受到其表面化学结构的限制。掺杂结构是改善生物炭表面化学性质的关键。本研究以兔毛为原料，通过热解和Fe4(P2O7)3活化制备了N，S，Fe自掺杂生物炭去除Cr（VI）。结果表明，Fe-RBC成功地保留了角蛋白中的含氧官能团和N官能团，同时生成SO42−和s2−，并引入Fe2 +。Cr（VI）去除率研究结果表明，1.2 g/L生物炭在6 h内对废水中Cr（VI）的去除率约为98.33%。XPS和CV结果表明，碳骨架构成了稳定的导电和吸附基底。S参与并促进Fe （II）和Fe （III）的循环，然后作为直接的电子传递介质还原Cr（VI）。石墨-n向碳骨架π体系贡献电子，得到了显著增强的芳香π电子云，通过阳离子-π相互作用为还原产物Cr（III）提供了吸附和固定位点。本研究充分利用蛋白质纤维的天然结构优势，实现了杂原子自掺杂生物炭的原位可控制备。

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引用次数: 0

Synergy between oxygenation and co-culture with liver sinusoidal endothelial cells drives expansion of functional liver organoids 氧合与肝窦内皮细胞共培养之间的协同作用驱动功能性肝类器官的扩张

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-07-01 Epub Date: 2026-03-03 DOI: 10.1016/j.bej.2026.110148

Takeshi Katsuda , Tia Utami , Ya Gong , Fumiya Tokito , Taketomo Kido , Kumiko Matsunaga , Atsushi Miyajima , Masaki Nishikawa , Yasuyuki Sakai

Induced pluripotent stem cell (iPSC)-derived hepatocyte-like cells (HLCs) hold great promise for drug discovery and cell therapy, but their immature phenotypes and limited proliferative capacity remain major challenges. We previously showed that direct oxygenation using an oxygen-permeable membrane enhances the differentiation of iPSC-derived liver progenitor cells (LPCs), although proliferation ceases during the terminal phase of maturation. Here, we demonstrate that combining direct oxygenation with co-culture of iPSC-derived liver sinusoidal endothelial cells (LSECs) overcomes this limitation. Under oxygenated conditions, co-cultured organoids exhibited robust increase in volume, whereas either oxygenation or co-culture alone was insufficient to support growth, indicating a synergistic effect. Importantly, oxygenated co-culture organoids maintained hepatic maturation comparable to oxygenated LPC monoculture organoids, based on albumin secretion and cytochrome P450 metabolic activities. Furthermore, oxygenation promoted spatial reorganization in co-cultured organoids, with LPCs occupying the center and LSECs forming a peripheral layer, suggesting improved architectural fidelity to the liver microenvironment. Together, this approach enables the scalable generation of highly mature liver organoids with enhanced structural and functional fidelity.

诱导多能干细胞（iPSC）衍生的肝细胞样细胞（hlc）在药物发现和细胞治疗方面具有很大的前景，但其不成熟的表型和有限的增殖能力仍然是主要的挑战。我们之前的研究表明，使用透氧膜的直接充氧增强了ipsc衍生的肝祖细胞（LPCs）的分化，尽管在成熟的终末阶段增殖停止。在这里，我们证明将直接氧合与ipsc来源的肝窦内皮细胞（LSECs）共同培养相结合克服了这一限制。在加氧条件下，共培养的类器官表现出强劲的体积增长，而单独加氧或共培养不足以支持生长，表明协同效应。重要的是，基于白蛋白分泌和细胞色素P450代谢活性，氧合共培养类器官维持的肝脏成熟程度与氧合LPC单培养类器官相当。此外，氧合促进了共培养类器官的空间重组，LPCs占据中心，LSECs形成外周层，表明提高了肝脏微环境的建筑保真度。总之，这种方法使高度成熟的肝类器官的可扩展生成具有增强的结构和功能保真度。

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引用次数: 0

Optimizing biomass and energy feedstock production from algal-bacterial biofilms treating swine wastewater: The role of organic carbon sources 优化藻类-细菌生物膜处理猪废水的生物质和能源原料生产：有机碳源的作用

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-07-01 Epub Date: 2026-03-08 DOI: 10.1016/j.bej.2026.110149

Qi Zhao , Tianxiang Lu , Jie Li , Xinglong Zhou , Chongqing Xu , Mingming Song , Ze Yu

This study establishes a novel strategy for the valorization of swine wastewater by co-producing lipid-rich and protein-rich microbial biomass using organic carbon-amended algal-bacterial biofilms. Among four carbon sources in the immobilized biofilm system, sodium acetate (C/N = 18) proved optimal, achieving a biomass productivity of 45.43 g/m²/d, which was 5.71 times greater than suspended co-cultures. Crucially, this system significantly enhanced the content and yield of valuable bioproducts, with lipid and protein contents reaching 30.46% and 42.39%, corresponding to yields of 110.70 g/m² and 154.06 g/m², respectively. The robust system performance also ensured high removal efficiencies for nutrients (NH₄⁺-N: 99.44%; TN: 98.02%; TP: 91.55%) and the antibiotic sulfamethazine (SM2: 75.63%), underscoring its efficacy in simultaneous wastewater treatment. Mechanistic investigations attributed the superior performance to sodium acetate-driven metabolic upregulation. This was marked by the extracellular accumulation of L-glutamate — a central metabolite in nitrogen assimilation — and hydrophobic amino acids, which collectively indicate a enhanced metabolic flux toward the synthesis of cellular proteins and lipids. This work demonstrates a sustainable, carbon-enhanced biofilm platform for the simultaneous recovery of energy (biodiesel precursor) and feed (protein) resources from swine wastewater.

本研究建立了一种利用有机碳修饰的藻-细菌生物膜共同生产富脂和富蛋白微生物生物量的新策略。在固定化生物膜系统的4种碳源中，以醋酸钠（C/N = 18）为最佳，生物量生产力为45.43 g/m²/d，是悬浮共培养的5.71倍。最重要的是，该体系显著提高了有价值生物制品的含量和产量，脂质和蛋白质含量分别达到30.46%和42.39%，对应的产量分别为110.70 g/m²和154.06 g/m²。稳健的系统性能还保证了对营养物（NH₄+ -N: 99.44%; TN: 98.02%; TP: 91.55%）和抗生素磺胺乙嗪（SM2: 75.63%）的高去除效率，强调了其在同时处理废水中的有效性。机制研究将这种优异的表现归因于醋酸钠驱动的代谢上调。这是由细胞外的l -谷氨酸（氮同化的中心代谢物）和疏水氨基酸的积累所标志的，它们共同表明细胞蛋白质和脂质合成的代谢通量增强。这项工作展示了一个可持续的、碳增强的生物膜平台，用于同时从猪废水中回收能源（生物柴油前体）和饲料（蛋白质）资源。

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引用次数: 0

Enhanced recovery of phenolic compounds from vegetable oil processing wastewater through a synergistic emulsion liquid membrane process 协同乳状液膜法提高植物油加工废水中酚类化合物的回收率

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-07-01 Epub Date: 2026-03-10 DOI: 10.1016/j.bej.2026.110163

Norela Jusoh , Izzat Naim Shamsul Kahar , Norasikin Othman , Norul Fatiha Mohamed Noah , Shuhada A. Idrus-Saidi , Muhammad Abbas Ahmad Zaini

Vegetable oil processing wastewater represents an underutilised bioresource rich in phenolic compounds (PCs) with high antioxidant potential. In this study, a synergistic emulsion liquid membrane (SELM) process was investigated to extract and recover PCs from palm oil mill sterilisation condensate by using a synergistic formulation of diluent (palm/sunflower oil), carrier (Aliquat 336/D2EHPA), surfactant (Span 80/Tween 80), and stripping agent (NaOH/Na₂CO₃). A two-level factorial design was first employed to screen the key operational parameters influencing the extraction performance. Then, four most significant parameters (mixed carrier and stripping agent concentrations, agitation speed, feed-to-emulsion ratio) were optimised by using Box-Behnken design to maximise the extraction performance. The effects of octanol as a modifier and feed phase concentration were studied to improve recovery performance. Under the optimal conditions of Aliquat 336/D2EHPA (0.2855/0.0023 M), NaOH/Na₂CO₃ (0.5129/0.0615 M), an agitation speed of 263 rpm and a feed-to-emulsion ratio of 2.94:1, the SELM process achieved an extraction performance of 91.2%. Modification of SELM with 5% w/v of octanol resulted in almost 80% recovery and 9.2 times solute enrichment. The results also demonstrated that the optimum formulation of SELM process remained effective up to 340 milligram gallic acid equivalents per liter (mg GAE/L) of feed phase concentration. These findings indicate that SELM process is promising for the valorisation of agro-industrial wastewater within resource recovery frameworks.

植物油加工废水是一种未被充分利用的富含酚类化合物（PCs）的生物资源，具有很高的抗氧化潜力。采用稀释剂（棕榈/葵花籽油）、载体（Aliquat 336/D2EHPA）、表面活性剂（Span 80/Tween 80）和汽提剂（NaOH/Na2CO3）的协同配方，研究了协同乳液液膜（SELM）工艺从棕榈油厂灭菌冷凝水中提取和回收pc。首先采用双水平析因设计筛选影响提取性能的关键操作参数。然后，通过Box-Behnken设计优化4个最重要的参数（混合载体和汽提剂浓度、搅拌速度、料乳比），以最大限度地提高提取性能。研究了辛醇作为改性剂和进料相浓度对提高回收性能的影响。在溶液浓度为336/D2EHPA（0.2855/0.0023 M）、NaOH/Na2CO3（0.5129/0.0615 M）、搅拌速度为263 rpm、料乳比为2.94:1的最佳条件下，SELM工艺提取率为91.2%。用5% w/v的辛醇对SELM进行改性，回收率接近80%，溶质富集9.2倍。结果还表明，SELM工艺的最佳配方在饲料相浓度达到340毫克没食子酸当量（mg GAE/L）时仍然有效。这些发现表明，SELM工艺有望在资源回收框架内实现农业工业废水的增值。

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引用次数: 0

Redirecting formic acid flux and balancing redox for high-yield succinic acid production in Actinobacillus succinogenes 琥珀酸放线杆菌甲酸通量重定向和氧化还原平衡高产琥珀酸生产

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-07-01 Epub Date: 2026-03-08 DOI: 10.1016/j.bej.2026.110159

Yuan Tian, Chunmei Chen, Xinglan Shi, Dan Wu, Pengcheng Chen, Pu Zheng

Succinic acid (SA) is an important organic dicarboxylic acid with broad applications in in chemical, pharmaceutical, and food industries. Actinobacillus succinogenes is a natural succinate producer considered a promising industrial strain. However, the formation of by-products such as formic acid (FA) and acetate acid (AA) during SA biosynthesis remains one of the major challenges. In this study, we focused on the role of FA metabolic branch in A. succinogenes. By inactivation of pyruvate formate-lyase using A. succinogenes base editors CBE, the strain that inhibited FA pathway named ΔpflB was obtained, and its growth, SA production, as well as intracellular nicotinamide adenine dinucleotide (NADH) were investigated. Subsequently, strategies were tried to restore NADH regeneration using overexpression of malate dehydrogenase(mdh), transhydrogenase(pntAB), and formate dehydrogenase(fdh) to regulate the intracellular NADH levels and NADH/NAD⁺ ratio of strain ΔpflB. We found that FA branch of A. succinogenes was critical for growth metabolic and redox balance in SA biosynthesis. Finally, through adaptive laboratory evolution to optimize the growth of strain ΔpflB, an evolved strain G100 with growth advantages was obtained after 100 generations. Compared with the parental strain, its SA yield increased by 37.2% and 39.2% in shake-flask and 3 L fermenter, respectively. Meanwhile, the intracellular NADH levels and the NADH/NAD⁺ ratio after evolution was significant adjusted during the fermentation process. The ΔpflB strain shows potential for industrial applications.

琥珀酸（SA）是一种重要的有机二羧酸，在化工、制药和食品工业中有着广泛的应用。琥珀酸放线菌是一种天然琥珀酸产菌，被认为是一种很有前途的工业菌株。然而，在SA生物合成过程中，甲酸（FA）和乙酸（AA）等副产物的形成仍然是主要挑战之一。在本研究中，我们重点研究了FA代谢分支在琥珀酸草中的作用。利用A. succinogenes碱基编辑器CBE对丙酮酸甲酸裂解酶进行失活，获得抑制FA通路的菌株ΔpflB，并对其生长、SA生成以及细胞内烟酰胺腺嘌呤二核苷酸（nicotinamide adenine dinucleotide， NADH）进行了研究。随后，我们尝试通过过表达苹果酸脱氢酶（mdh）、转氢酶（pntAB）和甲酸脱氢酶（fdh）来调节菌株ΔpflB细胞内NADH水平和NADH/NAD⁺的比值来恢复NADH再生。我们发现琥珀酸草FA分支在SA生物合成中对生长、代谢和氧化还原平衡至关重要。最后，通过自适应实验室进化对菌株ΔpflB的生长进行优化，100代后获得了具有生长优势的进化菌株G100。与亲本菌株相比，其SA产量在摇瓶和3 L发酵罐中分别提高了37.2%和39.2%。同时，在发酵过程中，细胞内NADH水平和进化后的NADH/NAD +比值被显著调节。ΔpflB菌株显示出工业应用的潜力。

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