Biochemical Engineering Journal最新文献

Assessing the environmental footprint of microalgae biofuel production: A comparative analysis of cultivation and harvesting scenarios 评估微藻生物燃料生产的环境足迹：种植和收获方案比较分析

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2024-11-15 DOI: 10.1016/j.bej.2024.109571

Yue Wang, Hao Wen, Meili Wu, Xu Liu, Hongwei Yin, Wei Qin, Xichen Zheng, Jia He, Kemin Wei, Xiaomin Kong, Shuhui Liang

This study investigated the impact of fixed combinations of three flocculants and four buoy-beads under two cultivation systems on the harvesting efficiency, as well as the environmental performance in different scenarios. Results showed that the harvesting efficiency exhibited a tendency of initially increasing and then decreasing with rising concentrations of buoy-beads and flocculants, with an optimal harvesting efficiency of 98.03 %. Life cycle assessment (LCA) compared the environmental performance of five scenarios. Ecotoxicity Soil Chronic (ESC) and Aquatic Eutrophication EP(P) (AEP(P)) were major environmental impacts. The scenario employing re-frying oil emulsion (RFOE) and aluminum sulfate flocculation (R+A) contributed significantly to Human Toxicity Water (HTW) and Aquatic Eutrophication EP(N) (AEP(N)) with normalized values of 0.0137 and 0.0147, respectively. In the assessment of Global Warming Potential (GWP), R+A was responsible for a high amount of Greenhouse Gas (GHG) emissions (2.826 kg CO₂ eq/100 g of dry algal biomass in photobioreactor (PBR) and 2.917 kg CO₂ eq/ 100 g of dry algal biomass in open raceway ponds (ORP)). Notably, sodium alginate microspheres (SAMs) and aluminum sulfate flocculation (S+A) was considered a more environmentally favorable option, 0.773 kg CO₂ eq and 0.864 kg CO₂ eq GHG emissions of PBR and ORP, respectively. Furthermore, the less GHG emissions of PBR than ORP, making it a more effective solution for reducing emissions and mitigating global warming trends.

本研究调查了两种栽培系统下三种絮凝剂和四种浮标珠的固定组合对收获效率的影响，以及不同情况下的环境绩效。结果表明，随着浮标珠和絮凝剂浓度的增加，收获效率呈现先升高后降低的趋势，最佳收获效率为 98.03%。生命周期评估（LCA）比较了五种方案的环境绩效。生态毒性土壤慢性（ESC）和水生富营养化 EP(P)（AEP(P)）是主要的环境影响。采用重炸油乳剂（RFOE）和硫酸铝絮凝剂（R+A）的方案对人类毒性水（HTW）和水生富营养化 EP(N) （AEP(N)）的影响很大，归一化值分别为 0.0137 和 0.0147。在全球变暖潜势（GWP）评估中，R+A 造成了大量温室气体（GHG）排放（在光生物反应器（PBR）中为 2.826 千克二氧化碳当量/100 克干海藻生物量，在开放式赛道池塘（ORP）中为 2.917 千克二氧化碳当量/100 克干海藻生物量）。值得注意的是，海藻酸钠微球（SAMs）和硫酸铝絮凝（S+A）被认为是更环保的选择，PBR 和 ORP 的温室气体排放量分别为 0.773 千克二氧化碳当量和 0.864 千克二氧化碳当量。此外，PBR 的温室气体排放量比 ORP 少，因此是减少排放和减缓全球变暖趋势的更有效解决方案。

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引用次数: 0

Bioenhancement mechanism of PVA-SA immobilized composite strains Alishewanella fetalis and Exiguobacterium profundum in pyridine degradation PVA-SA 固定化复合菌株胎儿阿利舍瓦氏菌和深层外杆菌降解吡啶的生物强化机制

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2024-11-14 DOI: 10.1016/j.bej.2024.109559

Min Gao , Yuan Shen , Yao Peng , Feiyang Tan , Yingshun Lv , Changjie Zhu , Yaxin Guo , Xuan Liu

Coal chemical wastewater (CCW) represents a type of recalcitrant organic wastewater characterized by its intricate composition and high concentration of pollutants, posing a severe threat to global aquatic environments and public health. This study focuses on the degradation of pyridine, a notoriously persistent organic pollutant in CCW, through the identification and application of two highly efficient pyridine-degrading bacterial strains: Alishewanella fetalis (Al-f3) and Exiguobacterium profundum (Ex-p). These strains were immobilized using a polyvinyl alcohol-sodium alginate (PVA-SA) matrix to investigate their bioaugmentation mechanisms in the pyridine degradation process. The findings indicate that strains Al-f3 and Ex-p achieved degradation rates of 95.94 % and 97.83 %, respectively, for an initial pyridine concentration of 200 mg/L at 96 hours. When strains Al-f3 and Ex-p were mixed in equal proportions and immobilized within PVA/SA beads, a degradation rate of 81.06 % was reached within 48 hours, with the efficiency increasing significantly by 96 hours. This enhancement is attributed primarily to the marked increase in enzymatic activity post-immobilization, achieving 17.13 μmol/mg·min, and the elevated secretion of extracellular proteins and polysaccharides, measured at 3.47 mg/L and 1.03 mg/L respectively within 48 hours. Notably, in the immobilized mixed culture system, the total organic carbon (TOC) was reduced to a mere 0.03 mg/L within 72 hours, with a removal rate of 92.31 %. These outcomes not only demonstrate the bioaugmentation role of the immobilized mixed strains in degrading pyridine but also offer novel solutions for the biodegradation of other organic contaminants in CCW, thereby enhancing the treatment efficiency of such wastewater. Given their large specific surface area and cost-effectiveness, PVA/SA immobilization matrices serve as efficient biocarriers in the biodegradation processes for treating CCW. This research provides innovative strategies and methods for the biotreatment of recalcitrant industrial wastewater.

煤化工废水（CCW）是一种难降解的有机废水，其特点是成分复杂、污染物浓度高，对全球水环境和公众健康构成严重威胁。本研究通过鉴定和应用两种高效的吡啶降解细菌菌株，重点研究 CCW 中臭名昭著的持久性有机污染物吡啶的降解问题：Alishewanella fetalis (Al-f3) 和 Exiguobacterium profundum (Ex-p)。这些菌株被固定在聚乙烯醇-海藻酸钠（PVA-SA）基质中，以研究它们在吡啶降解过程中的生物增强机制。研究结果表明，当初始吡啶浓度为 200 毫克/升时，Al-f3 和 Ex-p 菌株在 96 小时内的降解率分别为 95.94 % 和 97.83 %。将 Al-f3 和 Ex-p 菌株等比例混合并固定在 PVA/SA 珠子中，48 小时内降解率达到 81.06%，96 小时后降解率显著提高。这种提高主要归功于固定化后酶活性的显著提高（达到 17.13 μmol/mg- min），以及细胞外蛋白质和多糖分泌量的增加，48 小时内测得的分泌量分别为 3.47 mg/L 和 1.03 mg/L。值得注意的是，在固定化混合培养系统中，72 小时内总有机碳（TOC）降至仅 0.03 毫克/升，去除率达 92.31%。这些结果不仅证明了固定化混合菌株在降解吡啶方面的生物增效作用，还为生物降解 CCW 中的其他有机污染物提供了新的解决方案，从而提高了此类废水的处理效率。由于 PVA/SA 固定化基质具有较大的比表面积和成本效益，因此在处理 CCW 的生物降解过程中可作为高效的生物载体。这项研究为难降解工业废水的生物处理提供了创新的策略和方法。

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引用次数: 0

A novel lateral flow assay for lead ion detection based on G-quadruplex 基于 G 型四联体的新型横向流动铅离子检测分析法

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2024-11-14 DOI: 10.1016/j.bej.2024.109562

Linjiao Ren , Jingtong Sun , Shilin Ma , Diankang Wang , Rubin Qi , Pei Zhang , Qingfang Zhang , Zirui Qin , Liying Jiang

Lead ion residues pose potential health risks to humans. To develop a cost-effective and user-friendly portable lead ion detection method, a novel lateral flow test strip was designed and fabricated based on the G-quadruplex structure. Initially, G4 and its complementary strand antiG4 are in a double-stranded form in the assay solution. After adding lead ions, these ions compete with antiG4 to bind to G4 and form G-quadruplex structures, thus releasing a large number of antiG4 single strands. Through base pairing, one end of the antiG4 was linked to a recognition element containing gold nanoparticles, while the other end was captured by a test line probe, resulting in a red band on the test line. The color change of the test line was positively correlated with the concentration of lead ions. Results showed that by observing the color change of the test line under optimized experimental conditions, lead ion concentration could be detected with a visual detection limit of 20 nM. Quantitative analysis using ImageJ software indicated that the test strip had a linear detection range of 10–2000 nM, with a detection limit of 7.32 nM and significant specificity. The recovery rate in bottled drinking water ranged from 91.19 % to 126.04 %, providing a portable and simple new method for on-site detection of residual lead ions in water environments.

铅离子残留对人体健康构成潜在风险。为了开发一种经济高效、操作简便的便携式铅离子检测方法，我们设计并制作了一种基于 G-四重结构的新型横向流动检测条。最初，G4 及其互补链 antiG4 以双链形式存在于检测溶液中。加入铅离子后，这些离子与 antiG4 竞争，与 G4 结合形成 G-四链结构，从而释放出大量 antiG4 单链。通过碱基配对，抗 G4 的一端与含有金纳米粒子的识别元件相连，而另一端则被检测线探针捕获，从而在检测线上形成一条红带。测试线的颜色变化与铅离子的浓度呈正相关。结果表明，在优化的实验条件下，通过观察检测线的颜色变化，可以检测到视觉检测限为 20 nM 的铅离子浓度。使用 ImageJ 软件进行的定量分析表明，该测试条的线性检测范围为 10-2000 nM，检测限为 7.32 nM，具有显著的特异性。在瓶装饮用水中的回收率为 91.19 % 至 126.04 %，为现场检测水环境中的残留铅离子提供了一种便携、简单的新方法。

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引用次数: 0

Integrated strategies for engineering ferulic acid decarboxylase with tunnel conformation and substrate pocket for adapting non-natural substrates 阿魏酸脱羧酶工程的综合策略，具有适应非天然底物的隧道构象和底物口袋

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2024-11-14 DOI: 10.1016/j.bej.2024.109566

Xiaoni Zhu, Yanbin Feng, Mingdong Wang, Shang Li, Hongfei Li, Lin Liu, Song Xue

Ferulic acid decarboxylase (Fdc1) catalyzes the decarboxylation of ferulic acid derivatives from lignin with broad substrate spectra. However, the catalytic efficiency of Fdc1 across non-natural substrates with bulky substituents attached to the benzene ring limits its application. Here, an integrated strategy was developed to engineer ScFdc1 from Saccharomyces cerevisiae, specifically targeting 4-acetoxycinnamic acid for the production of 4-acetoxystyrene, which was widely used in photoresists monomers. The strategy entailed the integrating two structural aspects of the enzyme, the conformation of the access tunnel and the substrate binding pocket. Six mutants from a 1248-variant library with significant impacts on enzyme performance were identified. Integration of the two aspects of the mutants achieved the ScFdc1_F397V/I398L/T438P/P441V variant, exhibiting over an 11.8-fold activity improvement towards 4-acetoxycinnamic acid. The variant adopts an open tunnel conformation against that the closed state in the wild type, as revealed by structural analysis with the minimal distance at the bottleneck of the tunnel increased from 0.7 to 1.4 nm. Additionally, a reshaped binding pocket is identified which facilitates the substrate accessibility and binding affinity confirmed by enzymatic and molecular dynamics analysis. The results not only provide strategies for enzyme engineering through the accumulation of beneficial mutants, but also offer a promising route for sustainable styrene derivative production.

阿魏酸脱羧酶（Fdc1）可催化木质素中阿魏酸衍生物的脱羧反应，其底物谱很广。然而，Fdc1 对苯环上连接有笨重取代基的非天然底物的催化效率限制了其应用。在此，研究人员开发了一种综合策略，从酿酒酵母中设计 ScFdc1，专门针对 4-乙酰氧基肉桂酸生产 4-乙酰氧基苯乙烯，后者广泛用于光阻单体。该策略需要整合酶的两个结构方面，即进入隧道和底物结合口袋的构象。从 1248 个变体库中找出了对酶的性能有重大影响的六个变体。整合突变体的两个方面后，产生了 ScFdc1_F397V/I398L/T438P/P441V 变异体，对 4-乙酰氧基肉桂酸的活性提高了 11.8 倍以上。结构分析表明，与野生型的封闭状态相比，该变体采用了开放的隧道构象，隧道瓶颈处的最小距离从 0.7 纳米增加到 1.4 纳米。此外，还发现了一个重塑的结合口袋，通过酶学和分子动力学分析证实，该口袋有助于底物的可及性和结合亲和力。这些结果不仅通过积累有益的突变体为酶工程提供了策略，而且为苯乙烯衍生物的可持续生产提供了一条前景广阔的途径。

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引用次数: 0

Functionalized polyacrylonitrile nanofiber membranes with carbonic anhydrase for enhanced carbon dioxide capture and conversion: A performance study 含碳酸酐酶的功能化聚丙烯腈纳米纤维膜，用于增强二氧化碳捕获和转化：性能研究

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2024-11-13 DOI: 10.1016/j.bej.2024.109570

Shen-Yuan Yang , Jia-Yih Lin , Pei-Rong Li , Nguyen The Duc Hanh , Penjit Srinophakun , Bing-Lan Liu , Chen-Yaw Chiu , I-Son Ng , Kuei-Hsiang Chen , Yu-Kaung Chang

As industrial activity rises, atmospheric carbon dioxide levels have significantly contributed to global warming and climate change. Developing effective carbon dioxide capture technologies is imperative to mitigate these effects. Carbonic anhydrase (CA) enzymes represent one of the most promising solutions due to their rapid reaction rates, environmental safety, and efficiency in facilitating CO₂ conversion. This study takes a novel approach by investigating the immobilization of CA on functionalized polyacrylonitrile (PAN) nanofiber membranes to enhance CO₂ conversion and mineralization. PAN nanofibers were fabricated via electrospinning and chemically modified to introduce carboxylic groups, resulting in NM-COOH nanofiber membranes. In addition, amine groups from chitosan (CS) were incorporated to form NM-COOH-CS nanofiber membranes. The immobilization of CA on these membranes revealed that covalent attachment through NM-COOH significantly enhances catalytic activity compared to physical attachment methods. The NM-COOH-CA membrane exhibited superior performance, achieving efficient conversion of CO₂ into HCO₃⁻ and promoting CaCO₃ mineralization. It reached a precipitation efficiency of 11.77 mg CaCO₃ per gram of membrane-active unit (WAU) and maintained 63.12 % of its initial CaCO₃ production over five cycles over five weeks. This study presents a novel, eco-friendly approach to greenhouse gas reduction, emphasizing the effectiveness of CA-immobilized nanofiber membranes. The findings mainly highlight the advantages of carboxylic functional groups in enhancing CA performance, paving the way for future research in carbon capture technologies.

随着工业活动的增加，大气中的二氧化碳含量大大加剧了全球变暖和气候变化。为减轻这些影响，开发有效的二氧化碳捕获技术势在必行。碳酸酐酶（CA）因其快速的反应速度、环境安全性和促进二氧化碳转化的效率而成为最有前途的解决方案之一。本研究采用一种新方法，研究如何将碳酸酐酶固定在功能化聚丙烯腈（PAN）纳米纤维膜上，以提高二氧化碳的转化和矿化。通过电纺丝制造 PAN 纳米纤维，并对其进行化学改性以引入羧基，从而制成 NM-COOH 纳米纤维膜。此外，还加入了壳聚糖（CS）中的胺基团，形成了 NM-COOH-CS 纳米纤维膜。将 CA 固定在这些膜上的结果表明，与物理附着方法相比，通过 NM-COOH 进行共价附着能显著提高催化活性。NM-COOH-CA 膜性能优越，能将 CO2 高效转化为 HCO3-，并促进 CaCO3 矿化。它的沉淀效率达到每克膜活性单元（WAU）11.77 毫克 CaCO3，并在五周的五个循环中保持了其初始 CaCO3 产量的 63.12%。这项研究提出了一种减少温室气体的新型环保方法，强调了 CA 固定化纳米纤维膜的有效性。研究结果主要强调了羧基官能团在提高 CA 性能方面的优势，为未来碳捕集技术的研究铺平了道路。

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引用次数: 0

Synergistic acetyl-CoA augmentation strategy (SATS) for improved terpenoid biosynthesis in Saccharomyces cerevisiae 乙酰-CoA 增效战略（SATS）用于改善酿酒酵母中的萜类化合物生物合成

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2024-11-12 DOI: 10.1016/j.bej.2024.109572

Yiying Huo , Pan Feng , Haoran Bi , Kai Wang , Yang Zhang , Yunming Fang , Meng Wang , Tianwei Tan

Terpenoids are widely applied in pharmaceuticals, fragrances and biofuels. Acetyl-CoA, as a crucial precursor in the mevalonate pathway, is stringently regulated intracellularly, thereby limiting the biosynthesis of terpenoids. This study develops a synergistic acetyl-CoA augmentation strategy (SATS) to establish a robust platform for terpenoid biosynthesis in Saccharomyces cerevisiae. Optimizing coenzyme A biosynthesis via global regulation of the pantothenic acid module improved intracellular acetyl-CoA levels by 3.26-fold. Enhancing acetyl phosphate supply was achieved by constructing a PPP-PK-PTA pathway, which improved intracellular acetyl-CoA by 1.92-fold. By combining these techniques, acetyl-CoA levels in the SATS-engineered strain increased by 6.03-fold. The titer of amorpha-4,11-diene, a representative terpenoid, rose by 37.77-fold to 188.45 mg/L in shake-flask fermentation and reached 13 g/L in a 5 L bioreactor. Furthermore, the modified strain exhibited enhanced production of other terpenoids. Our research indicates SATS is an effective approach for synthesizing terpenoids and other acetyl-CoA-derived compounds, demonstrating broad applicability.

萜类化合物广泛应用于制药、香料和生物燃料领域。乙酰-CoA作为甲羟戊酸途径中的重要前体，在细胞内受到严格调控，从而限制了萜类化合物的生物合成。本研究开发了一种协同乙酰辅酶A扩增策略（SATS），为酵母中萜类化合物的生物合成建立了一个强大的平台。通过泛酸模块的全局调控优化辅酶 A 的生物合成，细胞内乙酰-CoA 水平提高了 3.26 倍。通过构建一个 PPP-PK-PTA 途径来增加乙酰磷酸的供应，从而将细胞内乙酰-CoA 的含量提高了 1.92 倍。结合这些技术，SATS 工程菌株的乙酰-CoA 水平提高了 6.03 倍。在摇瓶发酵中，具有代表性的萜类化合物 amorpha-4,11-二烯的滴度上升了 37.77 倍，达到 188.45 mg/L，在 5 L 生物反应器中达到 13 g/L。此外，改良菌株还提高了其他萜类化合物的产量。我们的研究表明，SATS 是合成萜类化合物和其他乙酰-CoA 衍生化合物的有效方法，具有广泛的适用性。

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引用次数: 0

The synergistic interaction of fungi with different structural characteristics improves the leaching of lithium from lepidolite 不同结构特征的真菌协同作用可提高锂从鳞片石中的沥滤效果

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2024-11-12 DOI: 10.1016/j.bej.2024.109564

Huaiyu Duan , Xingqing Zhao , Chao Xu , Du Zhang , Wei Gu , Rucheng Wang , Xiancai Lu

The aim of this study was to explore the improvement of lithium leaching from lepidolite by microbial co-culture, focusing on the synergistic effect of different structural fungi in improving the leaching performance of biological systems. The results showed that in the single leaching experiment, the multicellular fungi and the unicellular yeast showed weak effects. Multicellular fungus is limited by insufficient EPS secretion, while unicellular yeast is non-mycelial organisms with weak acid production capacity and limited effect on minerals. However, in the combined leaching experiment, the interspecific collaboration promoted the synthetic and metabolic activity of the two strains, resulting in changes in the type and content of organic acids, polysaccharides, proteins and humus. The content of citric acid reached the highest value of 16.98 g·L⁻¹ at about 22 d, and the EPS secreted by unicellular yeast promoted the mycelium adhesion and mineral wrapping of multicellular fungi. The combined action of the two enhanced the effects of acidification, complexation and mycelium destruction, and improved the leaching effect. This study revealed the synergistic metabolic mechanism of lepidolite leaching by fungi with different structures, verified the effectiveness of microbial co-culture to improve the leaching rate, and provided a basis for industrial application. In addition, the use of co-culture technology has a positive impact on commercial production and environmental protection.

本研究旨在探索微生物共培养对锂从鳞片石中浸出的改善作用，重点关注不同结构真菌在改善生物系统浸出性能方面的协同效应。结果表明，在单一浸出实验中，多细胞真菌和单细胞酵母菌的作用较弱。多细胞真菌受限于 EPS 分泌不足，而单细胞酵母菌是非菌丝生物，产酸能力弱，对矿物质的作用有限。但在联合浸出实验中，种间协作促进了两种菌株的合成代谢活动，使有机酸、多糖、蛋白质和腐殖质的种类和含量发生了变化。柠檬酸的含量在 22 d 左右达到最高值 16.98 g-L-1，单细胞酵母菌分泌的 EPS 促进了多细胞真菌的菌丝粘附和矿物包裹。两者的联合作用增强了酸化、络合和菌丝破坏的效果，提高了浸出效果。该研究揭示了不同结构真菌对鳞片石浸出的协同代谢机制，验证了微生物共培养对提高浸出率的有效性，为工业应用提供了依据。此外，共培养技术的使用还对商业生产和环境保护产生了积极影响。

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引用次数: 0

Effectiveness of Musa balbisiana bract toward chromium removal from industrial tannery effluent: Optimization, kinetics, isotherms, regeneration, and cost estimation 苞片麝香草从工业制革废水中去除铬的效果：优化、动力学、等温线、再生和成本估算

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2024-11-12 DOI: 10.1016/j.bej.2024.109565

Divya Baskaran , Becky Miriyam I , Palani R , Hun-Soo Byun

Eradicating chromium from industrial effluent is essential for environmental security and economic reasons. This study investigates the potency of activated Musa balbisiana bract biomass as a biosorbent to remove hexavalent chromium (Cr⁶⁺) from real industrial tannery effluent (ITE). The characterization study exemplifies the existence of irregular structures and excessive cavities, and the occurrence of functional groups (hydroxyl, carboxyl, esters, and alkynes) are benefitting the deposition of Cr⁶⁺ on the biosorbent. A maximum biosorption capacity of 42.75 ± 0.21 mg/g was observed at an optimum pH of 6.5, biosorbent dosage of 0.3 g, initial Cr⁶⁺ concentration of 50 mg/L, and contact time of 120 min. The validation experiment confirmed the removal efficiency of total chromium, trivalent chromium, and Cr⁶⁺ 92.56 ± 0.80 %, 98.63 ± 0.20 %, and 96.21 ± 0.50 %, respectively. Among the models, Langmuir isotherm (R²: 0.9992) and pseudo-second order (R²: 0.9999) kinetic models greatly correlate with the equilibrium data. A 2-tier membrane module was examined for continuous study and reached 88.23 ± 0.60 % Cr⁶⁺ removal. Statistical analysis was performed to confirm the significance of adsorption results. The likelihood of the desorption and regeneration of the treated biosorbent was investigated. The estimated cost per volume of ITE treated and unit of pollutant removal from ITE employing Musa balbisiana bract biosorbent is around $3.08/m³ and $3.75/kg.

消除工业废水中的铬对环境安全和经济发展至关重要。本研究调查了活性苞片麝香草生物质作为生物吸附剂去除实际工业制革废水（ITE）中六价铬（Cr6+）的有效性。表征研究表明，不规则结构和过多空腔的存在以及官能团（羟基、羧基、酯和炔基）的出现有利于 Cr6+ 在生物吸附剂上的沉积。在最佳 pH 值为 6.5、生物吸附剂用量为 0.3 克、初始 Cr6+ 浓度为 50 毫克/升、接触时间为 120 分钟时，观察到最大生物吸附容量为 42.75 ± 0.21 毫克/克。验证实验证实，总铬、三价铬和 Cr6+ 的去除率分别为 92.56 ± 0.80 %、98.63 ± 0.20 % 和 96.21 ± 0.50 %。在这些模型中，Langmuir 等温线模型（R2：0.9992）和伪二阶动力学模型（R2：0.9999）与平衡数据有很大的相关性。对 2 层膜组件进行了连续研究，其对 Cr6+ 的去除率达到了 88.23 ± 0.60%。统计分析证实了吸附结果的重要性。研究了经处理的生物吸附剂解吸和再生的可能性。使用苞片麝香草生物吸附剂处理 ITE 的单位体积和去除 ITE 中污染物的单位成本估计分别约为 3.08 美元/立方米和 3.75 美元/千克。

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引用次数: 0

Simultaneous endogenous partial denitrification/anammox process for low-strength wastewater treatment: Process optimization, nitrogen removal and microbial dynamics 用于低浓度废水处理的同步内源部分反硝化/氨氧化工艺：工艺优化、脱氮和微生物动力学

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2024-11-09 DOI: 10.1016/j.bej.2024.109568

Dong Li , Fanxu Zeng , Songwei Yang , Yuliang Zhu , Zhu Li , Huiping Zeng , Jie Zhang

The endogenous partial denitrification process (EPD) led by glycogen-accumulating organisms (GAOs) has become an alternative to NO₂⁻ supply in mainstream anaerobic-ammonia oxidation (anammox). However, low autotrophic nitrogen removal contribution is an urgent problem that needs to be solved in simultaneous endogenous partial denitrification/anammox (EPDA) system. This study used anaerobic duration optimization to enhance the autotrophic nitrogen removal capacity of EPDA system. The results showed that the EPDA activity increased from 0.67 to 1.09 mg N/g VSS·h after anaerobic time was extended to 120 min. This significantly improved the contribution of anammox to TIN removal, increasing from 23.5 % to 61.6 %. During the phase Ⅲ, Eff.TIN of 4.5±1.8 mg/L and NRE of 92.2 %±3.0 %. The enrichment of AnAOB (Candidatus Brocadia) and GAOs (Defluviicoccus) was responsible for maintaining the stability of the EPDA process. This study provides a feasible optimization strategy for improving the contribution of autotrophic nitrogen removal in the EPDA system.

由糖原累积生物（GAOs）主导的内源部分反硝化过程（EPD）已成为主流厌氧氨氧化（anammox）过程中氮氧化物供应的替代方式。然而，在同时进行的内源部分反硝化/氨氧化（EPDA）系统中，自养脱氮贡献率低是一个亟待解决的问题。本研究利用厌氧时间优化来提高 EPDA 系统的自养脱氮能力。结果表明，厌氧时间延长到 120 分钟后，EPDA 活性从 0.67 mg N/g VSS-h 提高到 1.09 mg N/g VSS-h。这大大提高了anammox对TIN去除的贡献率，从23.5%提高到61.6%。在第Ⅲ阶段，Eff.TIN 为 4.5±1.8 mg/L，NRE 为 92.2 %±3.0 %。AnAOB（Candidatus Brocadia）和 GAOs（Defluviicoccus）的富集是维持 EPDA 过程稳定的原因。这项研究为提高 EPDA 系统自养脱氮的贡献率提供了可行的优化策略。

{"title":"Simultaneous endogenous partial denitrification/anammox process for low-strength wastewater treatment: Process optimization, nitrogen removal and microbial dynamics","authors":"Dong Li , Fanxu Zeng , Songwei Yang , Yuliang Zhu , Zhu Li , Huiping Zeng , Jie Zhang","doi":"10.1016/j.bej.2024.109568","DOIUrl":"10.1016/j.bej.2024.109568","url":null,"abstract":"<div><div>The endogenous partial denitrification process (EPD) led by glycogen-accumulating organisms (GAOs) has become an alternative to NO<sub>2</sub><sup>−</sup> supply in mainstream anaerobic-ammonia oxidation (anammox). However, low autotrophic nitrogen removal contribution is an urgent problem that needs to be solved in simultaneous endogenous partial denitrification/anammox (EPDA) system. This study used anaerobic duration optimization to enhance the autotrophic nitrogen removal capacity of EPDA system. The results showed that the EPDA activity increased from 0.67 to 1.09 mg N/g VSS·h after anaerobic time was extended to 120 min. This significantly improved the contribution of anammox to TIN removal, increasing from 23.5 % to 61.6 %. During the phase Ⅲ, Eff.TIN of 4.5±1.8 mg/L and NRE of 92.2 %±3.0 %. The enrichment of AnAOB (<em>Candidatus Brocadia</em>) and GAOs (<em>Defluviicoccus</em>) was responsible for maintaining the stability of the EPDA process. This study provides a feasible optimization strategy for improving the contribution of autotrophic nitrogen removal in the EPDA system.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"213 ","pages":"Article 109568"},"PeriodicalIF":3.7,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142654701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biodegradation of deep eutectic solvent pre-treated natural rubber gloves by Klebsiella aerogenes: A sustainable approach to rubber waste management 产气克雷伯氏菌对深共晶溶剂预处理天然橡胶手套的生物降解：橡胶废物管理的可持续方法

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2024-11-08 DOI: 10.1016/j.bej.2024.109569

Harika Chittella , Li Wan Yoon , Suganti Ramarad , Zee-Wei Lai

This study investigates the potential of deep eutectic solvents (DES) to enhance the biodegradation of natural rubber gloves (NRG) by Klebsiella aerogenes. Choline chloride and urea (ChCl: urea) was the DES employed to pre-treat NRG at various temperatures (80°C to 140°C) and durations (0.5 h to 5 h). Pre-treated rubber (p-NRG) underwent significant physical and chemical changes, enhancing its biodegradability. Analytical techniques such as dry weight analysis, bacteria cell concentration, FTIR TGA, and SEM were used to characterize the pre-treated and biodegraded samples. The results have demonstrated a significant weight loss and structural modifications in p-NRG, with the highest degradation of 43 % observed at 140°C for 5 hours of pretreatment before biodegradation. Meanwhile, merely 17 % of weight loss was observed when pre-treatment was not employed. DES pre-treatment notably enhanced NRG biodegradability, achieving a 50.6 % weight loss when biodegradation was conducted at pH 7 and 35°C. The highest cell concentration, 0.75 g/L, was recorded in the second week of the biodegradation process. Results have indicated that the maximum protein concentration of 697.3 µg/ml, along with the highest enzyme activities for laccase and manganese peroxidase (MnP) at 0.46 ± 0.05 IU and 0.30 ± 0.05 IU respectively, were recorded in the second week of the biodegradation process. DES pre-treatment has significantly improved the biodegradability of NRG by Klebsiella aerogenes, offering a promising and eco-friendly solution for rubber waste management.

本研究探讨了深共晶溶剂（DES）在增强气肿克雷伯氏菌对天然橡胶手套（NRG）的生物降解方面的潜力。氯化胆碱和尿素（氯化胆碱：尿素）是用于预处理 NRG 的 DES，预处理温度（80°C 至 140°C）和持续时间（0.5 小时至 5 小时）各不相同。预处理后的橡胶（p-NRG）发生了显著的物理和化学变化，提高了其生物降解性。分析技术包括干重分析、细菌细胞浓度、傅立叶变换红外热像仪、热重分析和扫描电镜，用于描述预处理和生物降解样品的特性。结果表明，p-NRG 的重量明显减少，结构也发生了改变，在生物降解前，预处理温度为 140°C，时间为 5 小时，降解率最高，达到 43%。与此同时，在未采用预处理的情况下，仅观察到 17% 的重量损失。DES 预处理显著提高了 NRG 的生物降解性，在 pH 值为 7、温度为 35°C 的条件下进行生物降解时，重量损失率为 50.6%。在生物降解过程的第二周，细胞浓度最高，达到 0.75 克/升。结果表明，在生物降解过程的第二周，蛋白质浓度最高，为 697.3 µg/ml ，漆酶和锰过氧化物酶（MnP）的酶活性最高，分别为 0.46 ± 0.05 IU 和 0.30 ± 0.05 IU。DES 预处理大大提高了 NRG 在产气克雷伯氏菌作用下的生物降解能力，为橡胶废物管理提供了一种前景广阔的生态友好型解决方案。

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引用次数: 0