Biochemical Engineering Journal最新文献

Urea-based treatment promotes the propagation of antibiotic resistance genes during sludge fermentation: Insights into its multifaceted roles in structure disruption, microbial community reshaping, and metabolic regulation 基于尿素的处理促进了污泥发酵过程中抗生素抗性基因的繁殖：深入了解其在结构破坏，微生物群落重塑和代谢调节中的多方面作用

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-01-30 DOI: 10.1016/j.bej.2026.110101

Minjun Zhao , Shuaijie Jin , Wenzhuo Li , Yuke Xu , Qin Zhang

Urea has been documented as an excellent promoter for improving sewage sludge (SS) fermentation, considering its effectiveness and economic feasibility, yet its effects on the fates of antibiotic resistance genes (ARGs) during this process are still unknown. Herein, the responses of ARGs distribution to urea exposure were studied, and the results revealed that urea exacerbated ARGs propagation, as evidenced by an increase of 66.8 % total abundance. Mechanistic exploration demonstrated that the presence of urea and free ammonia (FA) stripped the extracellular polymeric substances (EPS) and increased the cell membrane permeability, contributing to ARGs and mobile genetic elements (MGEs) release and consequently improved their horizontal transfer. Also, urea exhibited “screening effects” to enrich some harboring ARGs carriers (e.g., Bacteroidetes_norank, Tissierella and Firmicutes_norank). Further analysis found that the generated FA induced oxidative stress (e.g., katE and SOD1) and activated the SOS response (e.g., recA, recO, and recR), promoting ARGs formation, which could be further improved by unhydrolyzed urea through upregulating the metabolic functions (e.g., TCA cycle) associated with energy production. The structural equation model suggested that the upregulation of key metabolic pathways was the predominant contributor to the ARGs propagation. Collectively, this work explored the effects and underlying mechanisms of urea on ARGs' fates during SS fermentation, highlighting the potential environmental risks of urea-based treatment on resource recovery from SS.

考虑到其有效性和经济可行性，尿素已被证明是改善污水污泥（SS）发酵的良好促进剂，但在此过程中其对抗生素抗性基因（ARGs）命运的影响尚不清楚。研究了尿素胁迫下ARGs分布的变化规律，结果表明尿素胁迫下ARGs的繁殖速度加快，总丰度增加66.8% %。机制探索表明，尿素和游离氨（FA）的存在剥离了细胞外聚合物（EPS），增加了细胞膜的通透性，促进了ARGs和移动遗传元件（MGEs）的释放，从而促进了它们的水平转移。此外，尿素对一些携带ARGs的载体（如Bacteroidetes_norank、Tissierella和Firmicutes_norank）具有“筛选作用”。进一步分析发现，生成的FA诱导氧化应激（如katE和SOD1），激活SOS反应（如recA、recO和recR），促进ARGs的形成，而未水解尿素可通过上调与能量产生相关的代谢功能（如TCA循环）进一步改善ARGs的形成。结构方程模型表明，关键代谢途径的上调是ARGs繁殖的主要因素。总之，本研究探讨了尿素对SS发酵过程中ARGs命运的影响及其潜在机制，强调了尿素处理对SS资源回收的潜在环境风险。

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引用次数: 0

Genetically encoded FRET-based sensor for intracellular heparin monitoring in real time 基因编码的基于fret的细胞内肝素实时监测传感器

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-01-27 DOI: 10.1016/j.bej.2026.110099

Sana Masroor, Neha Soleja, Mohamad Aman Jairajpuri, Mohd Mohsin

Heparin is an indispensable anticoagulant commonly administered during surgical interventions that require extracorporeal blood circulation, including cardiopulmonary bypass surgery. However, heparin overdose frequently leads to potentially fatal bleeding complications. Hence, precise monitoring of heparin levels is critical to avoid risks such as heparin-induced thrombocytopenia (HIT) and hemorrhage. Recently, Various detection tools have been designed for heparin, but these have complex procedures, low sensitivity, and do not perform real-time monitoring of heparin. Therefore, we have designed a highly effective genetically encoded fluorescence resonance energy transfer (FRET)-based Heparin Sensor (HepSen) to perform real-time monitoring of heparin with high spatio-temporal resolution. HepSen was developed by incorporating heparin-binding protein, platelet factor 4 (PF4) protein, between ECFP and Venus at N- and C-terminus, respectively. This novel nanosensor exhibits high stability at physiological pH levels and remains unaffected by the addition of biologically significant metal ions. Furthermore, two mutant variants, C12G and R20A, were also designed; nevertheless, wild-type HepSen showed the highest binding affinity with K_d of 2.73 × 10⁻⁷ M and a broad detection range of 1 nM-50 µM. HepSen exhibited robust expression in Escherichia coli (E. coli), Saccharomyces cerevisiae (S. cerevisiae), and human embryonic kidney (HEK-293T) cells, facilitating real-time monitoring of heparin with a well-defined saturation curve, thereby demonstrating its intracellular sensing capability across diverse biological systems.

肝素是一种不可缺少的抗凝剂，通常在需要体外血液循环的外科手术中使用，包括体外循环手术。然而，肝素过量经常导致潜在的致命出血并发症。因此，精确监测肝素水平对于避免肝素诱发的血小板减少症（HIT）和出血等风险至关重要。近年来，人们设计了多种肝素检测工具，但这些工具程序复杂，灵敏度低，不能实时监测肝素。因此，我们设计了一种高效的基于基因编码荧光共振能量转移（FRET）的肝素传感器（HepSen），以实现高时空分辨率的肝素实时监测。HepSen是通过将肝素结合蛋白、血小板因子4 （PF4）蛋白分别置于ECFP和Venus的N端和c端而开发的。这种新型纳米传感器在生理pH水平下具有很高的稳定性，并且不受添加具有生物意义的金属离子的影响。此外，还设计了C12G和R20A两个突变体；然而，野生型HepSen的结合亲和力最高，Kd为2.73 × 10−7 M，检测范围为1 nM-50µM。HepSen在大肠杆菌（E. coli）、酿酒酵母（S. cerevisiae）和人胚胎肾（HEK-293T）细胞中表现出强大的表达，通过定义明确的饱和曲线促进对肝素的实时监测，从而证明了其在不同生物系统中的细胞内传感能力。

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引用次数: 0

Innovatively engineered biowaste-derived CaO–Fe/activated carbon and ce/sepiolite composite catalysts for microwave-intensified biodiesel production from waste cooking oil 创新设计的生物垃圾衍生的CaO-Fe /活性炭和ce/海泡石复合催化剂，用于从废食用油中微波强化生产生物柴油

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-01-26 DOI: 10.1016/j.bej.2026.110097

Hiba A. Abdulkareem , Mohammed T. Yassen , Nooralhuda N. Ahmed , Malak. B.Ahmed , Saba A. Gheni , Mudheher M. Ali , Farah T. Al-Sudani , Ataallah K. Tahah

The development of low-cost and sustainable catalytic systems is essential for advancing biodiesel production from waste lipids. This study presents two novel heterogeneous catalysts derived entirely from natural and biowaste resources: (i) CaO–Fe supported on activated carbon produced from eggshells and date palm fronds (CaO–Fe/AC), and (ii) cerium-modified activated sepiolite (Ce/AS). The catalysts were synthesized via incipient wetness impregnation followed by thermal activation and comprehensively characterized using BET, SEM, XRD, FTIR, TGA, and ICP-OES analyses. The CaO–Fe/AC catalyst exhibited strong basicity and high surface area favorable for transesterification, while the Ce/AS catalyst provided bifunctional acid–base properties enabling the direct conversion of high–free fatty acid (FFA) waste cooking oil (WCO) through simultaneous esterification and transesterification. Process intensification using microwave irradiation significantly reduced reaction time and energy consumption. Under optimized conditions (methanol-to-oil ratio of 9:1, catalyst loading of 0.3 wt%, and 100 % microwave power), biodiesel conversions of 97 % for CaO–Fe/AC and 99 % for Ce/AS were achieved within 6 min. The produced biodiesel met ASTM D6751 and EN 14214 specifications. The combination of microwave-assisted heating and biowaste-derived composite catalysts offers a rapid, energy-efficient, and environmentally sustainable route for biodiesel production, supporting circular economy strategies and scalable renewable fuel technologies.

开发低成本和可持续的催化系统是推进废脂生物柴油生产的关键。本研究提出了两种完全来自自然和生物废弃物资源的新型多相催化剂：(i)蛋壳和椰枣叶活性炭负载的CaO-Fe （CaO-Fe /AC）和（ii）铈修饰的活化海泡石（Ce/AS）。采用初湿浸渍-热活化法制备催化剂，并用BET、SEM、XRD、FTIR、TGA、ICP-OES等方法对催化剂进行了综合表征。CaO-Fe /AC催化剂具有强碱性和高表面积，有利于酯交换；Ce/AS催化剂具有酸碱双功能，可通过同时酯化和酯交换直接转化高游离脂肪酸废食用油（WCO）。微波辐照强化工艺显著缩短了反应时间和能耗。在优化条件下（甲醇油比为9:1，催化剂负载为0.3 wt%，微波功率为100% %），在6 min内，CaO-Fe /AC和Ce/AS的生物柴油转化率分别达到97 %和99 %。生产的生物柴油符合ASTM D6751和EN 14214规格。微波辅助加热和生物废物衍生的复合催化剂的结合为生物柴油的生产提供了一条快速、节能和环境可持续的途径，支持循环经济战略和可扩展的可再生燃料技术。

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引用次数: 0

Hydrogel-based colorimetric sensor using dual-enzyme active MnFe-O/C nanozymes for on-site detection of phenolic pollutants in water 基于双酶活性MnFe-O/C纳米酶的水凝胶比色传感器用于水中酚类污染物的现场检测

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-01-25 DOI: 10.1016/j.bej.2026.110098

YangTao Yuan , JiaJia Mi , QiWei Li , JianPing Shi

The development of portable and reliable sensors for monitoring phenolic pollutants in water remains a significant challenge. Nanozyme-based colorimetric assays offer a promising alternative to conventional methods. However, their practical application is often hindered by poor portability and stability in liquid-phase systems. To address this, we developed a novel hydrogel-based colorimetric sensor. This sensor is empowered by a dual-enzyme active nanozyme. It is designed for the on-site detection of hydroquinone (HQ) and catechol (CC). The carbon-coated MnFe oxide (MnFe-O/C) nanoparticles, derived from a MnFe-Prussian blue analogue precursor, exhibits good intrinsic oxidase- and peroxidase-like activities. Leveraging the inhibitory effect of HQ and CC on the nanozyme-catalyzed chromogenic reaction, a sensitive solution-phase colorimetric assay was established. This assay achieved detection limits of 5.78 μM for HQ and 10.43 μM for CC. Furthermore, a portable and standalone sensor was fabricated by embedding the MnFe-O/C nanozyme within a borax-crosslinked carboxymethyl cellulose hydrogel network. When coupled with a smartphone for RGB analysis, the hydrogel sensor enables on-site and quantitative detection of HQ and CC in real water samples. The recovery rates in spiked water samples ranged from 94.80 % to 99.14 % for HQ and 97.32 %-108.80 % for CC, demonstrating high accuracy and reliability. This work not only offers a viable approach for constructing dual-enzyme active nanozymes, but also provides a practical tool for detecting phenolic pollutants in water.

开发便携式、可靠的监测水中酚类污染物的传感器仍然是一个重大挑战。纳米酶为基础的比色测定提供了一种有希望的替代传统方法。然而，它们在液相系统中的可移植性和稳定性差，往往阻碍了它们的实际应用。为了解决这个问题，我们开发了一种新型的基于水凝胶的比色传感器。这种传感器由一种双酶活性纳米酶驱动。它是专为现场检测对苯二酚（HQ）和儿茶酚（CC）。碳包覆的MnFe氧化物（MnFe- o /C）纳米颗粒，来源于MnFe-普鲁士蓝类似前体，具有良好的内在氧化酶和过氧化物酶样活性。利用HQ和CC对纳米酶催化的显色反应的抑制作用，建立了一种灵敏的液相比色法。该方法对HQ和CC的检出限分别为5.78 μM和10.43 μM。此外，将MnFe-O/C纳米酶包埋在硼砂交联羧甲基纤维素水凝胶网络中，制备了便携式独立传感器。当与智能手机相结合进行RGB分析时，水凝胶传感器可以在实际水样中进行HQ和CC的现场和定量检测。加样回收率为：HQ为94.80 % ~ 99.14 %，CC为97.32 % ~ 108.80 %，具有较高的准确度和可靠性。这项工作不仅为构建双酶活性纳米酶提供了可行的方法，而且为水中酚类污染物的检测提供了实用的工具。

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引用次数: 0

Removal of Fe2 + from acid mine drainage by Rhodopseudomonas sphaeroides-modified lignite: Adsorption characteristics and mechanism 球形红假单胞菌改性褐煤对酸性矿山废水中Fe2 +的吸附特性及机理

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-01-23 DOI: 10.1016/j.bej.2026.110095

Wenbo An , Bin Xu , Junzhen Di , Yifan Liu , Tianzhi Wang

Addressing the environmental toxicity of high-concentration heavy metal pollutants, such as Fe²⁺, in acid mine drainage (AMD) and the limited adsorption capacity of lignite, this study utilized lignite as a substrate adsorbent. By selecting the environmentally non-toxic Rhodopseudomonas sphaeroides, a novel low-cost modified adsorbent (Rhodopseudomonas sphaeroides-modified lignite, RS-L). Using batch experiments and microscopic characterization methods, the adsorption characteristics and mechanism of RS-L towards Fe²⁺ were investigated. Results indicated that natural lignite with a mesh size of 60–80 was modified with 12 mL of bacterial solution for 14 days to synthesize RS-L. When the initial Fe²⁺ concentration was 65 mg/L, pH was 4, and the dosage of RS-L was 1 g, optimal adsorption performance was achieved. The adsorption process followed the pseudo-second-order kinetic model and Langmuir monolayer adsorption, and was a spontaneous (negative ΔG), endothermic (positive ΔH), entropy-increasing (positive ΔS) process. At 45°C, the maximum adsorption capacity of Fe²⁺ was 13.10 mg/g. The adsorption mechanism primarily involved electrostatic adsorption, the packing effect, chemical precipitation, and complexation reactions. After five adsorption cycles, RS-L maintained an adsorption efficiency of 63.62 % and an adsorption capacity of 8.27 mg/g, demonstrating adaptability to complex ionic environments. This study offers new insights for restoring AMD and provides novel pathways for the resource utilization of lignite.

针对酸性矿井水（AMD）中高浓度重金属污染物（如Fe2+）的环境毒性和褐煤的有限吸附能力，本研究利用褐煤作为底物吸附剂。通过选择环保无毒的球形红假单胞菌，制备了一种新型的低成本改性吸附剂（球形红假单胞菌改性褐煤，RS-L）。采用批量实验和微观表征方法，研究了RS-L对Fe2+的吸附特性和机理。结果表明，用12 mL的细菌溶液修饰60-80孔径的天然褐煤，反应14 d即可合成RS-L。当初始Fe2+浓度为65 mg/L， pH为4，RS-L用量为1 g时，吸附效果最佳。吸附过程遵循拟二级动力学模型和Langmuir单层吸附，为自发（负ΔG）、吸热（正ΔH）、熵递增（正ΔS）过程。在45℃时，Fe2+的最大吸附量为13.10 mg/g。吸附机理主要包括静电吸附、填料效应、化学沉淀和络合反应。经过5次循环吸附后，RS-L的吸附效率为63.62 %，吸附容量为8.27 mg/g，对复杂离子环境具有较强的适应性。该研究为AMD的修复提供了新的见解，并为褐煤资源利用提供了新的途径。

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引用次数: 0

Advances in bioactivities, cultivation strategies, genetic engineering, and multifaceted applications of Euglena gracilis 细叶菊的生物活性、栽培策略、基因工程及多方面应用研究进展

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-01-21 DOI: 10.1016/j.bej.2026.110087

Zhuoyao Li , Hongyu Zhang , Wenjun Song , Tingting Lou , Suying Wang , Sisi Niu , Jun Liu , Jin Hua

Euglena gracilis (E. gracilis) is a unique single-celled eukaryotic microalga that lacks a rigid cell wall, which endows it with remarkable morphological plasticity and environmental resilience. It has attracted extensive attention from both academia and industry owing to its rich repertoire of bioactive metabolites, exceptional adaptability to fluctuating environments, and broad biotechnological potential. E. gracilis synthesizes a wide range of bioactive components, including β-carotene, paramylon, vitamins, and fatty acids. Moreover, it uniquely combines the photosynthetic autotrophy typical of plant cells with the heterotrophic metabolism characteristic of animal cells, providing a model for cross-kingdom metabolic integration and enhancing its ecological competitiveness. However, despite its capability for autotrophic, heterotrophic, and mixotrophic cultivation, its large-scale commercial exploitation remains challenged by environmental sensitivity and limited market recognition. This review systematically outlines the morphology, bioactivities, and cultivation strategies of E. gracilis, critically assessing how growth conditions influence biomass and metabolite profiles. Furthermore, advances in genetic engineering aimed at enhancing strain robustness, productivity, and functional compound biosynthesis are reviewed, along with their emerging applications in food, health, bioenergy, and environmental fields. Collectively, this work highlights the value of E. gracilis as a multifunctional and sustainable biological platform, providing a scientific and practical foundation for its future industrial applications.

细叶藻（Euglena gracilis， E. gracilis）是一种独特的单细胞真核微藻，缺乏坚硬的细胞壁，这使其具有显著的形态可塑性和环境适应性。由于其丰富的生物活性代谢物、对波动环境的特殊适应性和广泛的生物技术潜力，它引起了学术界和工业界的广泛关注。薄叶菊合成了广泛的生物活性成分，包括β-胡萝卜素、paramylon、维生素和脂肪酸。此外，它独特地将植物细胞的光合自养特性与动物细胞的异养代谢特性结合起来，为跨界代谢整合提供了模型，增强了其生态竞争力。然而，尽管它具有自养、异养和混合养的能力，但其大规模商业开发仍然受到环境敏感性和市场认可度有限的挑战。这篇综述系统地概述了薄叶菊的形态、生物活性和培养策略，批判性地评估了生长条件如何影响生物量和代谢物特征。此外，综述了旨在提高菌株稳健性、生产力和功能性化合物生物合成的基因工程的进展，以及它们在食品、健康、生物能源和环境领域的新应用。综上所述，本研究突出了薄叶菊作为多功能可持续生物平台的价值，为其未来的工业应用提供了科学和实践基础。

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引用次数: 0

ZrO2/g-C3N4 heterojunction nanocomposites for enhanced photocatalytic degradation of organic pollutants and antibacterial activity ZrO2/g-C3N4异质结纳米复合材料增强光催化降解有机污染物和抗菌活性

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-01-19 DOI: 10.1016/j.bej.2026.110085

Shweta Vashisth, S.P. Nehra

In the current study, zirconium oxide/graphitic carbon nitride (ZrO₂/g-C₃N₄) nanocomposites have been synthesized using Murraya koenigii leaf extract as an eco-friendly reducing and stabilizing agent. The properties of the ZrO₂/g-C₃N₄ nanocomposites were investigated by employing several analytical techniques such as X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), X-ray photoelectron spectroscopy (XPS), high-resolution transmission electron microscopy (HRTEM), UV-Vis diffuse reflectance spectroscopy (UV-DRS), field emission scanning electron microscopy (FESEM), energy-dispersive X-ray analysis (EDX), and elemental mapping. The photocatalytic activity of the nanocomposites was evaluated through the use of ultraviolet-visible (UV-Vis) spectroscopy for the removal of the organic dyes, xylenol orange (XO) and malachite green (MG) dyes. Following a thorough analysis, it has been demonstrated that, in comparison to other samples, the ZrCN 2 nanocomposite showed significantly enhanced photocatalytic dye degradation activity for malachite green (94.31 %) and xylenol orange (59.28 %) dyes. To support the potential use of the synthesized nanomaterials against Escherichia coli, an antibacterial study has also been conducted. ZrCN 2 showed the highest antibacterial activity against E. coli compared to the other samples, with an 8-mm zone of inhibition and the lowest minimum inhibitory concentration (30 µg). The composite's recyclability test has been performed for up to five cycles, and its photodegradation results remained consistent. Moreover, a scavenger experiment was conducted to determine the major reactive oxygen species (ROS) involved in the photocatalytic mechanism. The outcomes of the present investigation offer important insights into the synthesis and characterization of ZrO₂/g-C₃N₄ nanocomposites that demonstrate enhanced photocatalytic dye degradation and antibacterial efficiency as compared to pure g-C₃N₄ and ZrO₂ nanoparticles.

本研究以木树叶提取物为还原剂和稳定剂，合成了氧化锆/石墨氮化碳（ZrO2/g-C3N4）纳米复合材料。采用x射线衍射（XRD）、傅里叶变换红外光谱（FTIR）、热重分析（TGA）、x射线光电子能谱（XPS）、高分辨率透射电子显微镜（HRTEM）、紫外-可见漫反射光谱（UV-DRS）、场发射扫描电子显微镜（FESEM）、能量色散x射线分析（EDX）和元素映射等分析技术研究了ZrO2/g-C3N4纳米复合材料的性能。利用紫外-可见（UV-Vis）光谱法评价了纳米复合材料对有机染料、二甲酚橙（XO）和孔雀石绿（MG）染料的光催化活性。经过深入的分析，与其他样品相比，zrcn2纳米复合材料对孔雀石绿（94.31 %）和二甲酚橙（59.28 %）染料的光催化降解活性显著增强。为了支持合成的纳米材料对抗大肠杆菌的潜在用途，还进行了一项抗菌研究。ZrCN 2对大肠杆菌的抑菌活性最高，抑菌带为8 mm，最低抑菌浓度为30 µg。复合材料的可回收性测试已经进行了多达五个循环，其光降解结果保持一致。此外，还进行了清除剂实验，以确定参与光催化机制的主要活性氧（ROS）。本研究的结果为ZrO2/g-C3N4纳米复合材料的合成和表征提供了重要的见解，与纯g-C3N4和ZrO2纳米颗粒相比，它们具有更好的光催化染料降解和抗菌效率。

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引用次数: 0

Sustainable valorization of rapeseed meal to bioactive and nutritional products via solid-state fermentation with protease-enhanced Streptomyces sp. SCUT-3 利用蛋白酶增强链霉菌SCUT-3固态发酵将菜籽粕持续发酵为具有生物活性和营养价值的产品

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-01-19 DOI: 10.1016/j.bej.2026.110086

Zhen-Jie Dai , Wen-Jing Feng , Can Qin , Hua-Hong Cai , Jing-Yi Hu , Zhi-Wei Li , Ming-Shu Zhang , Lei Wu , Jun-Jin Deng , Xiao-Chun Luo

To meet the growing global demand for protein driven by population increases, converting agricultural by-products into edible protein is critical for sustainable food security. This study introduces a solid-state fermentation (SSF) platform utilizing protease-engineered Streptomyces sp. SCUT-3 for the valorization of rapeseed meal (RSM), the world’s second-largest oilseed residue. Optimized SSF parameters (substrate-liquid ratio 1:2 w/v, 2-day fermentation) achieved 82.2 % protein recovery rate, yielding 15.3 g free amino acids (FAAs) and 17.8 g soluble peptides (SPPs) (96 % < 2000 Da) per 100 g RSM (40.28 g protein). Fermented RSM (FRSM) showed significant improvements: 86.5 % protein digestibility (a 2.6-fold enhancement), and a more enriched nutritional profile with 47.4 % essential amino acids in FAAs. Additionally, SSF effectively reduced anti-nutritional factors, removing 92 % glucosinolates and nearly 100 % phytic acid. Structural analysis revealed that FRSM’s fragmented, porous morphology facilitated improved nutrient absorption. A largemouth bass feeding trial demonstrated the practical benefits of FRSM, where a 10 % replacement in feed increased weight gain rate and feed intake compared to RSM-fed controls. This study provides a scalable and sustainable solution for the valorization of RSM, addressing both protein security and circular economy objectives.

为了满足由人口增长驱动的全球对蛋白质日益增长的需求，将农业副产品转化为可食用蛋白质对可持续粮食安全至关重要。本研究介绍了一种利用蛋白酶工程Streptomyces sp. SCUT-3的固态发酵（SSF）平台，用于世界第二大油籽渣菜籽粕（RSM）的发酵。优化后的SSF参数（底液比1:2 w/v，发酵2天）的蛋白质回收率为82.2 %，每100 g RSM（40.28 g蛋白质）产生15.3 g游离氨基酸（FAAs）和17.8 g可溶性肽（SPPs）（96 % < 2000 Da）。发酵的RSM （FRSM）表现出显著的改善：蛋白质消化率为86.5 %（提高2.6倍），营养成分更丰富，FAAs中必需氨基酸含量为47.4 %。此外，SSF有效地降低了抗营养因子，去除92% %的硫代葡萄糖苷和近100% %的植酸。结构分析表明，FRSM的破碎多孔形态有助于提高营养物质的吸收。一项大口黑鲈饲养试验证明了FRSM的实际效益，在饲料中添加10% %的替代饲料，与rsm饲养的对照组相比，可提高增重率和采食量。该研究为RSM的增值提供了一个可扩展和可持续的解决方案，同时解决了蛋白质安全和循环经济目标。

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引用次数: 0

A first newly isolated strain capable of effectively degrading 3-methylpentane: Identification, degradation characteristics and pathway analysis 一个新分离的能有效降解3-甲基戊烷的菌株：鉴定、降解特性和途径分析

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-01-19 DOI: 10.1016/j.bej.2026.110084

Qian Li , Yujing Zhao , Liqin Sun , Zhonghui Sun , Raquel Lebrero , Raúl Muñoz , Jianmeng Chen , Rongsheng Lin , Dongzhi Chen

Biotechnology is applicable for the treatment of volatile organic compounds emitted from petrochemical industries. However, the biodegradation of methyl alkanes is limited by low microbial activity and poor mass transfer. Herein, a novel strain capable of effectively utilizing 3-methylpentane (3MP) as the sole carbon source was firstly isolated and identified as Mycobacterium sp. SLQ-1. The strain could function well within wide ranges of pH (5.0–8.0) and temperature (30–45℃), with the maximum specific growth and biodegradation rates being 0.084 and 0.587 h⁻¹, respectively. The optimum function conditions (pH 6.9, temperature 36.2℃) and 3MP removal efficiencies were predicted via response surface analysis. The repeated recycling of 2-methyl silicone oil enhanced 3MP biodegradation due to microbial adsorption. Two possible 3MP biodegradation pathways were proposed based on the analysis of metabolic intermediates, genes and enzymes. Furthermore, the strain had a wide utilization spectrum toward various alkanes, olefins and aromatics, presenting great engineering application potential.

生物技术适用于石油化工排放的挥发性有机物的处理。然而，甲基烷烃的生物降解受到微生物活性低和传质差的限制。本文首次分离到一株能有效利用3-甲基戊烷（3MP）作为唯一碳源的新菌株，并鉴定为分枝杆菌sp. SLQ-1。菌株在较宽的pH（5.0 ~ 8.0）和温度（30 ~ 45℃）范围内均能很好地生长，其最大比生长率和生物降解率分别为0.084和0.587 h−1。通过响应面分析，预测了最佳作用条件（pH 6.9，温度36.2℃）和3MP去除效率。2-甲基硅油的重复循环利用通过微生物吸附增强了3MP的生物降解。通过对代谢中间体、基因和酶的分析，提出了两种可能的3MP生物降解途径。该菌株对各种烷烃、烯烃和芳烃具有广泛的利用谱，具有很大的工程应用潜力。

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引用次数: 0

Enhanced biodegradation of plastics through engineered two-stage anaerobic co-digestion: Integrating organic waste valorization 通过工程两阶段厌氧共消化增强塑料的生物降解：整合有机废物增值

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-01-16 DOI: 10.1016/j.bej.2026.110083

Penaganti Praveen, Debabrata Mazumder

The rapid increase in plastic production has led to a corresponding rise in plastic pollution, posing significant environmental challenges. The management of mixed plastic waste, which typically requires complex separation processes, could be simplified by co-digesting plastic waste with organic waste, such as fruit and vegetable waste, in an anaerobic digester. While some studies have explored the anaerobic digestion of plastics, the biodegradation rates are typically low, rendering it an inefficient solution for large-scale plastic waste treatment. To address this limitation, this study investigates the potential of a two-stage anaerobic co-digestion process with an experimental period of 330 days, emphasizing the role of hydrolytic bacteria, which are crucial for virgin plastic beads and plastic waste degradation. Results showed a reduction in methane production, ranging from 10.1 % to 39.4 % in the co-digestion batch compared to the control batch. However, the biodegradation rates were significantly higher than those observed in previous anaerobic digestion studies of plastic waste. Notably, the highest biodegradation rate was observed for polyvinyl chloride waste (21.01 %), while the lowest was for high-density polyethylene beads (13.02 %). Further confirmation of these findings was provided by SEM and FTIR analyses, which revealed distinct signs of plastic biodegradation. Additionally, microbial community analysis and carbon mass balance calculations were performed, further validating the enhanced biodegradability in this two-stage anaerobic digestion system.

塑料产量的快速增长导致了塑料污染的相应增加，对环境构成了重大挑战。混合塑料废物的管理通常需要复杂的分离过程，通过在厌氧消化器中将塑料废物与有机废物（如水果和蔬菜废物）共消化，可以简化塑料废物的管理。虽然一些研究已经探索了塑料的厌氧消化，但生物降解率通常很低，使其成为大规模塑料废物处理的低效解决方案。为了解决这一限制，本研究研究了两阶段厌氧共消化过程的潜力，实验期为330天，强调水解细菌的作用，这对原生塑料珠和塑料废物的降解至关重要。结果表明，与对照批次相比，共消化批次的甲烷产量降低了10. %至39.4 %。然而，生物降解率明显高于之前在塑料废物厌氧消化研究中观察到的结果。值得注意的是，聚氯乙烯废物的生物降解率最高（21.01 %），而高密度聚乙烯珠的生物降解率最低（13.02 %）。SEM和FTIR分析进一步证实了这些发现，显示了塑料生物降解的明显迹象。此外，进行了微生物群落分析和碳质量平衡计算，进一步验证了该两阶段厌氧消化系统的生物降解性。

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引用次数: 0