Biochemical Engineering Journal最新文献

英文中文

Feeding regime selectively enriching acetoclastic methanogens to enhance energy production in anaerobic digestion systems

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-04-21 DOI: 10.1016/j.bej.2025.109764

Huanhuan Chang , Qidong Yin , Kai He , Jo De Vrieze , Guangxue Wu

Applying the r/K selection theory to explore the distribution patterns of acetoclastic methanogens under varying conditions is rare, although it can offer a basis for optimizing community structure and enhancing methane production in anaerobic digestion systems. Different operating modes (sequencing batch reactors, SBRs; and continuous-flow reactors, CFRs) and solids retention times (SRTs; 15 and 50 days) were adopted to acclimate different acetoclastic methanogens in acetate-fed anaerobic reactors. SBRs exhibited a significantly higher CH₄ production rate than CFRs (P = 0.037). Methanosarcina exhibited a higher relative abundance in SBRs (13.7 ∼ 16.1 %) than in CFRs (0.2 ∼ 0.3 %), aligning with its typical r-strategist characteristics. Methanothrix showed a higher enrichment in CFRs (33.1 ∼ 39.6 %) compared to SBRs (26.8 ∼ 29.9 %) at the same SRT, indicating K-strategist behavior. The SBRs had the potential to co-enrich both types of methanogens. Feeding regimes played a more pivotal role in the distribution of methanogens than SRT. The dominant bacteria, such as Desulfococcus and Mesotoga, as well as the archaeon Methanothrix, were auxotrophic in some essential amino acids, implying potential cross-feeding interactions. This study provides key insights into ecological strategies by linking microbiology with environmental technologies to enrich target methanogenic communities and enhance methane production.

{"title":"Feeding regime selectively enriching acetoclastic methanogens to enhance energy production in anaerobic digestion systems","authors":"Huanhuan Chang , Qidong Yin , Kai He , Jo De Vrieze , Guangxue Wu","doi":"10.1016/j.bej.2025.109764","DOIUrl":"10.1016/j.bej.2025.109764","url":null,"abstract":"<div><div>Applying the r/K selection theory to explore the distribution patterns of acetoclastic methanogens under varying conditions is rare, although it can offer a basis for optimizing community structure and enhancing methane production in anaerobic digestion systems. Different operating modes (sequencing batch reactors, SBRs; and continuous-flow reactors, CFRs) and solids retention times (SRTs; 15 and 50 days) were adopted to acclimate different acetoclastic methanogens in acetate-fed anaerobic reactors. SBRs exhibited a significantly higher CH<sub>4</sub> production rate than CFRs (<em>P</em> = 0.037). <em>Methanosarcina</em> exhibited a higher relative abundance in SBRs (13.7 ∼ 16.1 %) than in CFRs (0.2 ∼ 0.3 %), aligning with its typical r-strategist characteristics. <em>Methanothrix</em> showed a higher enrichment in CFRs (33.1 ∼ 39.6 %) compared to SBRs (26.8 ∼ 29.9 %) at the same SRT, indicating K-strategist behavior. The SBRs had the potential to co-enrich both types of methanogens. Feeding regimes played a more pivotal role in the distribution of methanogens than SRT. The dominant bacteria, such as <em>Desulfococcus</em> and <em>Mesotoga</em>, as well as the archaeon <em>Methanothrix</em>, were auxotrophic in some essential amino acids, implying potential cross-feeding interactions. This study provides key insights into ecological strategies by linking microbiology with environmental technologies to enrich target methanogenic communities and enhance methane production.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"220 ","pages":"Article 109764"},"PeriodicalIF":3.7,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143864947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fermentation optimization and metabolomic analysis of ε-polylysine production in Streptomyces albulus

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-04-18 DOI: 10.1016/j.bej.2025.109758

Yuanyuan Yu , Li Xia , Jianping Wen

ε-Polylysine (ε-PL) is a natural preservative with broad-spectrum antibacterial activity and has been widely used as a green food additive. However, the low yield and high cost of wild-type strains have restricted its industrial development. In this study, the production of ε-PL by Streptomyces albulus CICC11022 under different medium components and fermentation conditions was compared. The yield of ε-PL was increased from 0.70 g/L to 2.89 g/L, a 3.1-fold increase. Metabolomics analysis indicated that nitrogen source, as a key factor in increasing yield, mainly exerted its effect by enhancing the synthesis of L-lysine, the precursor of ε-PL, via the alpha aminoadipic acid (AAA) pathway. Based on this result, exogenous addition of L-lysine increased the concentration of ε-PL to 4.13 g/L. This is the relatively high yield of ε-PL produced by shake-ﬂask fermentation at present, and provides a reference for improving the industrial production of ε-PL.

引用次数: 0

Nitrogen removal performance and feeding strategy optimisation in a nitritation denitrification step-feed sequencing batch reactor system

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-04-18 DOI: 10.1016/j.bej.2025.109761

Yihan Wang , Zhenjun Wu , Qingwen Qin , Lijie Chen , Ming Cai , Xin Chen , Yutong Liu , Ziang Su , Xinyi Fan , Lang Cheng

A 90-day experiment was conducted to optimise the stability of Nitritation Denitrification and improve COD utilisation. The results showed that Nitritation Denitrification in a step-feed sequencing batch reactor using the influent-anoxic/aerobic (IA/O) mode can achieve efficient and stable nitrogen removal at C/N = 4. The removal efficiencies of NH₄⁺-N and total inorganic nitrogen (TIN) were 95 % and 85.5 % respectively. Batch experiments were conducted that an unlimited increase in the number of step feeds in a limited time would affect the nitrogen removal. Extending the duration of the aerobic phase inhibited Flavobacterium and favoured the relative abundance of the nirS and nirK genes. This study may provide a reference for optimising the Nitritation Denitrification process.

引用次数: 0

Whole-cell catalytic synthesis of cadaverine by recombinant Corynebacterium glutamicum using corncob residue as carbohydrate feedstock

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-04-17 DOI: 10.1016/j.bej.2025.109760

Ying-Ying Xu, Bin Zhang, Jie Bao

Industrial production of cadaverine primarily relies on whole-cell catalysis of lysine using engineered Escherichia coli as host cells. The growing interest in cadaverine as a monomer of biobased polyamides requires inexpensive, non-food carbohydrate feedstock for its bioproduction, rather than using food-derived sugars as feedstock. This study used corncob residue as the starting carbohydrate feedstock followed by enzymatic hydrolysis to obtain the sugars. Since E. coli was not tolerant to even minor inhibitor residues in corncob residue hydrolysate, the robust Corynebacterium glutamicum was used as the host bacterium followed by the metabolic modifications of secretory expression of lysine decarboxylase via Ncgl1289 and cgR_0949, and cadaverine degradation pathway knockout. The final whole-cell catalysis of C. glutamicum recombinant using corncob hydrolysate as carbohydrate feedstock achieved a record-high 78.19 g/L of cadaverine with a conversion yield of 91 %.

引用次数: 0

The flow of proteins through the mammalian secretory pathway described by a phenomenological-based semi-physical model

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-04-17 DOI: 10.1016/j.bej.2025.109759

Santiago Benavides-López , Laura M. Muñoz-Echeverri , Mauricio A. Trujillo-Roldán , Norma A. Valdez-Cruz

In the present study, we developed a model for the transport of proteins across the secretory pathway using the Phenomenological Based Semi-Physical Model (PBSM) methodology. The model represents the bulk flow of proteins along the organelles of the mammalian secretory pathway and works considering the role played by the Ras GTPase family cycles in the control of the vesicle coat assembly, the recycle of v-SNARE between the organelles of the pathway, and the dynamical equilibrium between the endoplasmic reticulum and the Golgi complex volumes due to the constant flow of vesicle between organelles. We integrate literature kinetic data from human, hamster, and monkey cells for model validation. The model describes the bulk flow of luminal and membrane proteins, following the well-mixing assumption at the intra-organelle level and the mass action law principle, revealing information on the secretory pathway at a quantitative level.

引用次数: 0

Simulation of bioreactor flow environments for large-scale animal cell culture conditions relevant to cultivated meat production

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-04-11 DOI: 10.1016/j.bej.2025.109753

Kira Kiviat , David E. Block , Harishankar Manikantan

Cultivated meat has the potential to mitigate many detrimental effects of conventional meat production on land use and greenhouse gas emissions. However, to meet an increasing demand for sustainable protein sources and to achieve cost parity with conventionally grown meat, animal cells will likely be required to be produced in bioreactors at an order of magnitude larger scale than has been done so far. To help de-risk this scale up, simulations of plausible bioreactor configurations were performed at a series of scales ranging from 200 L to 200,000 L using computational fluid dynamics. Several different bubble drag models were compared, and the one that predicted the lowest mass transfer and highest shear was used in order to be conservative about the predicted cell environment. The distributions of shear stress, oxygen mass transfer coefficient, and Kolmogorov length scale were compared across the bioreactor scales, showing only minor changes with increasing scale. The case of a Rushton and pitched impeller was compared to a case with two Rushton impellers, and the latter was found to have higher mass transfer and only slightly higher shear for a given power input. This study provides a step towards predicting animal cell culture performance at the scales needed for sustainable cultivated meat production.

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引用次数: 0

Rapid detection of Staphylococcus aureus in animal-derived foods using aptamer-based fluorescent lateral flow biosensors

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-04-11 DOI: 10.1016/j.bej.2025.109756

Shuxian Zhou , Yinghui Wang , Haifang Wang , Shouyi Dou , Mengyue Liu , Haowei Dong , Zhengtao Li , Donghan Li , Jingjing Liu , Xia Sun , Yemin Guo , Ibrahim A. Darwish , Yanfang Wu

Staphylococcus aureus (S. aureus), commonly found in animal-derived foods, poses a significant threat to food safety. Traditional detection methods often suffer from limited sensitivity and selectivity, are time-consuming, and involve complex procedures. In this study, fluorescent lateral flow biosensors (LFB) based on aptamer recognition were developed for the rapid and cost-effective detection of S. aureus. The method utilizes a Cy5-labeled aptamer (Cy5-Apt) as a signal recognition molecule. Detection is achieved through the competitive binding of S. aureus and a complementary strand to the aptamer, enabling the fluorescence intensity measurement on the LFB within 10 min. Key factors including the Cy5-Apt concentration, the length of the linker arm, the position and length of the complementary strand to the aptamer, BSA concentration, and buffer composition were optimized. The method showed good linear response for the quantitative detection of S. aureus in the concentration range of 2.8 × 10¹ to 2.8 × 10⁷ CFU mL⁻¹, and the limit of detection (LOD) was 1.65 CFU mL⁻¹. Application tests in milk and chicken meat confirmed the selectivity and sensitivity of this enzyme- and antibody-free approach. This aptamer-based method offers a promising solution for low-cost, rapid, and efficient detection of S. aureus in food safety applications.

{"title":"Rapid detection of Staphylococcus aureus in animal-derived foods using aptamer-based fluorescent lateral flow biosensors","authors":"Shuxian Zhou , Yinghui Wang , Haifang Wang , Shouyi Dou , Mengyue Liu , Haowei Dong , Zhengtao Li , Donghan Li , Jingjing Liu , Xia Sun , Yemin Guo , Ibrahim A. Darwish , Yanfang Wu","doi":"10.1016/j.bej.2025.109756","DOIUrl":"10.1016/j.bej.2025.109756","url":null,"abstract":"<div><div><em>Staphylococcus aureus</em> (<em>S. aureus</em>), commonly found in animal-derived foods, poses a significant threat to food safety. Traditional detection methods often suffer from limited sensitivity and selectivity, are time-consuming, and involve complex procedures. In this study, fluorescent lateral flow biosensors (LFB) based on aptamer recognition were developed for the rapid and cost-effective detection of <em>S. aureus</em>. The method utilizes a Cy5-labeled aptamer (Cy5-Apt) as a signal recognition molecule. Detection is achieved through the competitive binding of <em>S. aureus</em> and a complementary strand to the aptamer, enabling the fluorescence intensity measurement on the LFB within 10 min. Key factors including the Cy5-Apt concentration, the length of the linker arm, the position and length of the complementary strand to the aptamer, BSA concentration, and buffer composition were optimized. The method showed good linear response for the quantitative detection of <em>S. aureus</em> in the concentration range of 2.8 × 10<sup>1</sup> to 2.8 × 10<sup>7</sup> CFU mL<sup>−1</sup>, and the limit of detection (LOD) was 1.65 CFU mL<sup>−1</sup>. Application tests in milk and chicken meat confirmed the selectivity and sensitivity of this enzyme- and antibody-free approach. This aptamer-based method offers a promising solution for low-cost, rapid, and efficient detection of <em>S. aureus</em> in food safety applications.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"220 ","pages":"Article 109756"},"PeriodicalIF":3.7,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143823285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel high-temperature Kluyveromyces marxianus as a microbial cell factory host for sesquiterpene production

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-04-11 DOI: 10.1016/j.bej.2025.109739

Min Zhang , Yongfu Ruan , Yi Hu , Yiying Huo , Meng Wang , Zhiwei Zhu , Yunming Fang

Artemisinin, a vital antimalarial agent, is traditionally extracted from Artemisia annua L. by plant-based extraction. The microbial biosynthesis of the artemisinin precursor, amorpha-4,11-diene (AD), followed by its chemical conversion into artemisinin provides a sustainable solution to meet the demand for artemisinin while overcoming the limitations of plant-based extraction. Kluyveromyces marxianus is a promising host for microbial cell factories owing to its robust thermotolerance, rapid growth kinetics, and ability to utilize a wide range of carbon sources. Herein, genetic modification tools for K. marxianus, including transformation methods and expression vectors, were identified and optimized, while the sesquiterpene biosynthetic capacity of K. marxianus was preliminarily evaluated. To enhance the precursor metabolite supply, eight key genes from the endogenous mevalonate pathway were overexpressed within the K. marxianus genome. A dual co-expression strategy, involving both extrachromosomal and chromosomally integrated expression of amorpha-4,11-diene synthase (ADS) was employed, followed by high-temperature fermentation to optimize production. Finally, an engineered K. marxianus strain was developed for the first time, capable of stable, efficient, and cost-effective production of AD, with a titer of 66.78 mg/L, a 113-fold increase compared with the wild-type strain. This engineered strain serves as a robust chassis and a valuable reference for the production and study of other terpenoids, laying the groundwork for further exploration of K. marxianus characteristics and the biosynthesis of AD.

{"title":"A novel high-temperature Kluyveromyces marxianus as a microbial cell factory host for sesquiterpene production","authors":"Min Zhang , Yongfu Ruan , Yi Hu , Yiying Huo , Meng Wang , Zhiwei Zhu , Yunming Fang","doi":"10.1016/j.bej.2025.109739","DOIUrl":"10.1016/j.bej.2025.109739","url":null,"abstract":"<div><div>Artemisinin, a vital antimalarial agent, is traditionally extracted from <em>Artemisia annua</em> L. by plant-based extraction. The microbial biosynthesis of the artemisinin precursor, amorpha-4,11-diene (AD), followed by its chemical conversion into artemisinin provides a sustainable solution to meet the demand for artemisinin while overcoming the limitations of plant-based extraction. <em>Kluyveromyces marxianus</em> is a promising host for microbial cell factories owing to its robust thermotolerance, rapid growth kinetics, and ability to utilize a wide range of carbon sources. Herein, genetic modification tools for <em>K. marxianus</em>, including transformation methods and expression vectors, were identified and optimized, while the sesquiterpene biosynthetic capacity of <em>K. marxianus</em> was preliminarily evaluated. To enhance the precursor metabolite supply, eight key genes from the endogenous mevalonate pathway were overexpressed within the <em>K. marxianus</em> genome. A dual co-expression strategy, involving both extrachromosomal and chromosomally integrated expression of amorpha-4,11-diene synthase (<em>ADS</em>) was employed, followed by high-temperature fermentation to optimize production. Finally, an engineered <em>K. marxianus</em> strain was developed for the first time, capable of stable, efficient, and cost-effective production of AD, with a titer of 66.78 mg/L, a 113-fold increase compared with the wild-type strain. This engineered strain serves as a robust chassis and a valuable reference for the production and study of other terpenoids, laying the groundwork for further exploration of <em>K. marxianus</em> characteristics and the biosynthesis of AD.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"220 ","pages":"Article 109739"},"PeriodicalIF":3.7,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143825997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Solar evaporation enabled household ethanol distillation and disinfection system for remote area

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-04-11 DOI: 10.1016/j.bej.2025.109757

Yijuan Wang , Ying Liao , Xiaojing Hou , Mingyang Shu , Liang Qian , Hao Li

Ethanol concentrations of 70 %–90 % are commonly used to prevent healthcare-associated infections due to their effectiveness against various pathogens. However, high costs and transportation challenges limit the availability of commercial ethanol disinfectants, particularly in low- and middle-income countries. Local recycling of waste ethanol could address these issues, but large-scale implementation is hindered by the need for specialized equipment. Here, a small-scale, solar-powered ethanol recycling and disinfection device designed for household use was developed. Recycled melamine sponges, coated with polydopamine, serve as solar evaporators. The system achieves high evaporation rates (1.52–7.54 kg m⁻² h⁻¹) for ethanol solutions ranging from 0 % to 100 %. Through multiple distillations, dilute ethanol can be concentrated to the required levels for medical disinfection. A closed-loop system recycles waste ethanol, and outdoor tests demonstrate its effectiveness in eliminating Escherichia coli and Candida albicans using solar-generated ethanol vapor. This work offers a sustainable solution for small-scale ethanol distillation and disinfection in remote areas.

浓度为 70%-90% 的乙醇由于对各种病原体有效，通常用于预防与医疗保健有关的感染。然而，高昂的成本和运输困难限制了商用乙醇消毒剂的供应，尤其是在中低收入国家。就地回收废乙醇可以解决这些问题，但由于需要专业设备，大规模实施受到阻碍。在此，我们开发了一种小型太阳能乙醇回收和消毒设备，专供家庭使用。涂有聚多巴胺的回收三聚氰胺海绵可用作太阳能蒸发器。该系统的乙醇溶液蒸发率很高（1.52-7.54 kg m-2 h-1），范围从 0% 到 100%。通过多次蒸馏，稀乙醇可以浓缩到医疗消毒所需的水平。一个闭环系统对废弃乙醇进行回收利用，室外测试表明，利用太阳能产生的乙醇蒸汽消除大肠杆菌和白色念珠菌非常有效。这项工作为偏远地区的小规模乙醇蒸馏和消毒提供了一个可持续的解决方案。

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引用次数: 0

Three-dimensional and serum-free culture in fixed-bed bioreactor enhance exosome production by affecting the cytoskeleton through integrin β1 and RAC1

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-04-11 DOI: 10.1016/j.bej.2025.109750

Chuanfu Huo , Yuanyuan Zhao , Meng Song , Yuzhe Guo , Shihao Li , Wensong Tan , Yan Zhou

Exosomes are nanoscale vesicles containing a variety of bioactive factors, showing significant potential as cell-free therapeutic agents. However, the large clinical doses required for exosome administration highlight the need for a scalable serum-free production system to increase exosome yield. In this study, human umbilical cord-derived mesenchymal stem cells (hUCMSCs) were cultured in three-dimensional (3D) environments using polyethylene terephthalate (PET) fiber membranes. Two key pathways promoting exosome secretion and biogenesis through 3D culture were identified. Specifically, 3D culture reduced the expression of Integrin β1, leading to the decondensation of cortical actin, and increased the expression of RAC1, which promoted actin aggregation on the membranes of multivesicular bodies (MVBs). Building on this mechanistic understanding, we simulated the depolymerization/aggregation of the cytoskeleton in 3D culture and designed a serum-free medium optimized for exosome production. For large-scale exosome production, hUCMSCs were cultured in a fixed-bed bioreactor using PET fiber rolls as the fixed-bed layer with the developed serum-free medium. This setup increased exosome production per cell by 16-fold compared to 2D culture, yielding 2.6 × 10^14 exosome particles. The exosome products demonstrated enhanced in vitro angiogenesis, immunomodulation, and in vivo wound healing capabilities. This study provides valuable insights and strategic guidance for the serum-free, large-scale production of exosomes using bioreactors, with significant implications for therapeutic applications.

{"title":"Three-dimensional and serum-free culture in fixed-bed bioreactor enhance exosome production by affecting the cytoskeleton through integrin β1 and RAC1","authors":"Chuanfu Huo , Yuanyuan Zhao , Meng Song , Yuzhe Guo , Shihao Li , Wensong Tan , Yan Zhou","doi":"10.1016/j.bej.2025.109750","DOIUrl":"10.1016/j.bej.2025.109750","url":null,"abstract":"<div><div>Exosomes are nanoscale vesicles containing a variety of bioactive factors, showing significant potential as cell-free therapeutic agents. However, the large clinical doses required for exosome administration highlight the need for a scalable serum-free production system to increase exosome yield. In this study, human umbilical cord-derived mesenchymal stem cells (hUCMSCs) were cultured in three-dimensional (3D) environments using polyethylene terephthalate (PET) fiber membranes. Two key pathways promoting exosome secretion and biogenesis through 3D culture were identified. Specifically, 3D culture reduced the expression of Integrin β1, leading to the decondensation of cortical actin, and increased the expression of RAC1, which promoted actin aggregation on the membranes of multivesicular bodies (MVBs). Building on this mechanistic understanding, we simulated the depolymerization/aggregation of the cytoskeleton in 3D culture and designed a serum-free medium optimized for exosome production. For large-scale exosome production, hUCMSCs were cultured in a fixed-bed bioreactor using PET fiber rolls as the fixed-bed layer with the developed serum-free medium. This setup increased exosome production per cell by 16-fold compared to 2D culture, yielding 2.6 × 10^14 exosome particles. The exosome products demonstrated enhanced <em>in vitro</em> angiogenesis, immunomodulation, and <em>in vivo</em> wound healing capabilities. This study provides valuable insights and strategic guidance for the serum-free, large-scale production of exosomes using bioreactors, with significant implications for therapeutic applications.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"220 ","pages":"Article 109750"},"PeriodicalIF":3.7,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143823286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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全部化学•材料生命科学医学物理工程技术环境•农林材料科学地球科学法学管理学化学环境科学与生态学计算机科学教育学经济学农林科学人文科学生物学数学物理与天体物理心理学综合性期刊其他工业工程理学历史学农学文学信息工程

数据库

全部 ACS Publications Elsevier ieeexplore Springer The Royal Society of Chemistry Wiley

期刊

Biochemical Engineering Journal

全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.

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