Biochemical Engineering Journal最新文献

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Synthesis of (R)-acetoin by targeted immobilization of acetolactate synthase and acetolactate decarboxylase via the spy/snoop system

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-02-19 DOI: 10.1016/j.bej.2025.109671

Kexin Shao , Yadong Wang , Peng Chen , Fenghuan Wang

Acetoin (3-hydroxy-2-butanone) is an important four-carbon platform compound and an intermediate for several high-value-added pharmaceutical compounds. In this study, Catcher-Tag tagged proteins were used for targeted immobilization of acetolactate synthase (ALS) and acetolactate decarboxylase (ALDC) for catalyzing the synthesis of Acetoin from sodium pyruvate in vitro. The study discussed the enzymatic properties of the immobilized enzyme LX@ALS-SpyTag 和 LX@ALDC-SnoopTag, as well as the optimal catalytic conditions and reusability of the immobilized dual enzyme in vitro catalytic system. The immobilized enzyme exhibited higher relative activity across a broader range of temperature and pH intervals than the free enzyme. The in vitro catalytic synthesis of (R)-acetoin from sodium pyruvate using an immobilized dual enzyme system resulted in 99.95 % of the theoretical conversion of (R)-acetoin at 45°C, pH 7.0, and 0.2 M substrate concentration. In addition, the immobilized dual enzyme system had better operational stability than the free enzyme, retaining 69.20 % of the initial total catalytic activity after 5 cycles. In conclusion, this study demonstrated a promising and convenient Catcher-Tag tagged protein immobilization strategy that enables rapid targeted immobilization of tagged proteins directly from crude enzyme solution, providing a novel approach for the construction of in vitro immobilized multi-enzyme complexes.

{"title":"Synthesis of (R)-acetoin by targeted immobilization of acetolactate synthase and acetolactate decarboxylase via the spy/snoop system","authors":"Kexin Shao , Yadong Wang , Peng Chen , Fenghuan Wang","doi":"10.1016/j.bej.2025.109671","DOIUrl":"10.1016/j.bej.2025.109671","url":null,"abstract":"<div><div>Acetoin (3-hydroxy-2-butanone) is an important four-carbon platform compound and an intermediate for several high-value-added pharmaceutical compounds. In this study, Catcher-Tag tagged proteins were used for targeted immobilization of acetolactate synthase (ALS) and acetolactate decarboxylase (ALDC) for catalyzing the synthesis of Acetoin from sodium pyruvate in vitro. The study discussed the enzymatic properties of the immobilized enzyme LX@ALS-SpyTag 和 LX@ALDC-SnoopTag, as well as the optimal catalytic conditions and reusability of the immobilized dual enzyme in vitro catalytic system. The immobilized enzyme exhibited higher relative activity across a broader range of temperature and pH intervals than the free enzyme. The in vitro catalytic synthesis of (R)-acetoin from sodium pyruvate using an immobilized dual enzyme system resulted in 99.95 % of the theoretical conversion of (R)-acetoin at 45°C, pH 7.0, and 0.2 M substrate concentration. In addition, the immobilized dual enzyme system had better operational stability than the free enzyme, retaining 69.20 % of the initial total catalytic activity after 5 cycles. In conclusion, this study demonstrated a promising and convenient Catcher-Tag tagged protein immobilization strategy that enables rapid targeted immobilization of tagged proteins directly from crude enzyme solution, providing a novel approach for the construction of in vitro immobilized multi-enzyme complexes.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"217 ","pages":"Article 109671"},"PeriodicalIF":3.7,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143445410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling the antibiotics removal ability of Monoraphidium contortum

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-02-17 DOI: 10.1016/j.bej.2025.109686

Larissa T.A. Costa-Ramos , Marcus V.X. Senra , Guilherme H.S. Nogueira , Ralf R. Ramalho-Júnior , Sandro J. Andrade , Evellin E. Santo , Aline K. Gohara-Beirigo , Juliana Gomes Santos , Livia S. Ferreira-Camargo , João Carlos M. Carvalho , Marcelo C. Matsudo

This study shows the Monoraphidium contortum potential for removing antibiotics, specifically sulfamethoxazole (SMX) and trimethoprim (TMP), from wastewater. Experiments were conducted using a bench-scale tubular photobioreactor, where the microalga demonstrated the ability to reduce antibiotic concentrations while simultaneously removing inorganic nutrients and producing commercially valuable biomass. When each antibiotic was individually added in the culture medium, M. contortum was able to remove 42.3 % of SMX and 28.6 % of TMP. However, when both antibiotics were introduced simultaneously, SMX removal decreased to 7 %. Additionally, the biochemical composition of the resulting biomass revealed stable lipid content, with a fatty acid profile suitable for biodiesel production. By genome analysis, it was possible to identify a multicopper oxidase enzyme, with potential antibiotic-degrading properties, shedding light on the mechanisms behind the removal process. These findings suggest that M. contortum could play a crucial role in bioremediation and sustainable wastewater treatment, generating feedstock for bioenergy production.

{"title":"Unveiling the antibiotics removal ability of Monoraphidium contortum","authors":"Larissa T.A. Costa-Ramos , Marcus V.X. Senra , Guilherme H.S. Nogueira , Ralf R. Ramalho-Júnior , Sandro J. Andrade , Evellin E. Santo , Aline K. Gohara-Beirigo , Juliana Gomes Santos , Livia S. Ferreira-Camargo , João Carlos M. Carvalho , Marcelo C. Matsudo","doi":"10.1016/j.bej.2025.109686","DOIUrl":"10.1016/j.bej.2025.109686","url":null,"abstract":"<div><div>This study shows the <em>Monoraphidium contortum</em> potential for removing antibiotics, specifically sulfamethoxazole (SMX) and trimethoprim (TMP), from wastewater. Experiments were conducted using a bench-scale tubular photobioreactor, where the microalga demonstrated the ability to reduce antibiotic concentrations while simultaneously removing inorganic nutrients and producing commercially valuable biomass. When each antibiotic was individually added in the culture medium, <em>M. contortum</em> was able to remove 42.3 % of SMX and 28.6 % of TMP. However, when both antibiotics were introduced simultaneously, SMX removal decreased to 7 %. Additionally, the biochemical composition of the resulting biomass revealed stable lipid content, with a fatty acid profile suitable for biodiesel production. By genome analysis, it was possible to identify a multicopper oxidase enzyme, with potential antibiotic-degrading properties, shedding light on the mechanisms behind the removal process. These findings suggest that <em>M. contortum</em> could play a crucial role in bioremediation and sustainable wastewater treatment, generating feedstock for bioenergy production.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"217 ","pages":"Article 109686"},"PeriodicalIF":3.7,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143430120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Degradation of β-HCH by Enterobacter sp. CS01: Characteristics, mechanism and application in soil remediation 肠杆菌 CS01 对 β-HCH 的降解：特性、机理及在土壤修复中的应用

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-02-15 DOI: 10.1016/j.bej.2025.109673

Huijun Shi , Qing Chen , Yanpeng Liang , Litang Qin , Honghu Zeng , Xiaohong Song

β-Hexachlorocyclohexane (β-HCH) is a persistent organochlorine pesticide that poses a significant threat to the ecological environment, necessitating the urgent development of effective degradation methods. Microbial degradation has demonstrated substantial potential among various bioremediation techniques due to its environmentally friendly and economical characteristics. This study evaluates the degradation capability of Enterobacter sp. CS01 on β-HCH, its physiological responses, and its potential application in soil remediation. Under optimal conditions (pH 7, 30°C), 51 % of β-HCH was effectively removed. Metabolomics and antioxidant enzyme activity analyses revealed that CS01 defends against oxidative damage by modulating the activities of superoxide dismutase (SOD) and catalase (CAT), involving butyrate, alanine, aspartate, and glutamate metabolism, as well as the pentose phosphate pathway. CS01 converts β-HCH into less toxic intermediates through dichloride elimination, dehalogenation of hydrogen, and hydrolysis reactions. Soil experiments indicated that soil enzyme activities (S-POD, S-DHA, S-PPO) are closely related to the degradation of β-HCH, with the order of carbon source utilization being esters, amino acids, and sugars. This study provides new insights into the microbial degradation mechanisms of organochlorine pesticides and aids in the development of more efficient and environmentally friendly degradation technologies.

{"title":"Degradation of β-HCH by Enterobacter sp. CS01: Characteristics, mechanism and application in soil remediation","authors":"Huijun Shi , Qing Chen , Yanpeng Liang , Litang Qin , Honghu Zeng , Xiaohong Song","doi":"10.1016/j.bej.2025.109673","DOIUrl":"10.1016/j.bej.2025.109673","url":null,"abstract":"<div><div>β-Hexachlorocyclohexane (β-HCH) is a persistent organochlorine pesticide that poses a significant threat to the ecological environment, necessitating the urgent development of effective degradation methods. Microbial degradation has demonstrated substantial potential among various bioremediation techniques due to its environmentally friendly and economical characteristics. This study evaluates the degradation capability of <em>Enterobacter</em> sp. CS01 on β-HCH, its physiological responses, and its potential application in soil remediation. Under optimal conditions (pH 7, 30°C), 51 % of β-HCH was effectively removed. Metabolomics and antioxidant enzyme activity analyses revealed that CS01 defends against oxidative damage by modulating the activities of superoxide dismutase (SOD) and catalase (CAT), involving butyrate, alanine, aspartate, and glutamate metabolism, as well as the pentose phosphate pathway. CS01 converts β-HCH into less toxic intermediates through dichloride elimination, dehalogenation of hydrogen, and hydrolysis reactions. Soil experiments indicated that soil enzyme activities (S-POD, S-DHA, S-PPO) are closely related to the degradation of β-HCH, with the order of carbon source utilization being esters, amino acids, and sugars. This study provides new insights into the microbial degradation mechanisms of organochlorine pesticides and aids in the development of more efficient and environmentally friendly degradation technologies.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"217 ","pages":"Article 109673"},"PeriodicalIF":3.7,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143422732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of electric field on microbial metabolism in petroleum-polluted soil: Insights from microbial function and carbon utilization characteristics 电场对石油污染土壤中微生物代谢的影响：从微生物功能和碳利用特征看问题

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-02-13 DOI: 10.1016/j.bej.2025.109665

Ruijuan Fan , Mengke Zhu , Bin Yang , Xingfu Yan

This study investigated how the electric field enhances microbial metabolism during bio-electrokinetic (BIO-EK) remediation and identified factors hindering sustainable pollutant degradation. The results showed that the degradation rate of total organic carbon (TOC) was higher in BIO-EK than in bioremediation and electrokinetic processes, by up to 2.22 and 1.54 times, respectively. Similarly, the degradation efficiency of total petroleum hydrocarbons (TPH) in BIO-EK was 1.58 and 1.81 times higher than in the bioremediation and electrokinetic groups. In BIO-EK, microbial biomass carbon (MBC) content was up to 6.13 times higher than in the bioremediation group, and dissolved organic carbon (DOC) content was 2.30 and 1.26 times higher than in the bioremediation and electrokinetic groups, respectively. This indicates that the electric field promoted the conversion of TOC to DOC, which was assimilated by microorganisms to generate MBC. The analysis of the structure and functional genes of soil microbial communities revealed that the electric field accelerated the degradation of key petroleum hydrocarbon components and the soil carbon cycle. However, degradation rates varied, and MBC and DOC levels declined in later stages, indicating discontinuous pollutant degradation. The analysis of the ratio of organic carbon derived from petroleum and soil sources (¹²Cp/¹²Cs) revealed intermittent use of petroleum-derived carbon, potentially limiting the sustainable degradation of pollutants. The research results provide insights for improving BIO-EK remediation efficiency.

{"title":"Effects of electric field on microbial metabolism in petroleum-polluted soil: Insights from microbial function and carbon utilization characteristics","authors":"Ruijuan Fan , Mengke Zhu , Bin Yang , Xingfu Yan","doi":"10.1016/j.bej.2025.109665","DOIUrl":"10.1016/j.bej.2025.109665","url":null,"abstract":"<div><div>This study investigated how the electric field enhances microbial metabolism during bio-electrokinetic (BIO-EK) remediation and identified factors hindering sustainable pollutant degradation. The results showed that the degradation rate of total organic carbon (TOC) was higher in BIO-EK than in bioremediation and electrokinetic processes, by up to 2.22 and 1.54 times, respectively. Similarly, the degradation efficiency of total petroleum hydrocarbons (TPH) in BIO-EK was 1.58 and 1.81 times higher than in the bioremediation and electrokinetic groups. In BIO-EK, microbial biomass carbon (MBC) content was up to 6.13 times higher than in the bioremediation group, and dissolved organic carbon (DOC) content was 2.30 and 1.26 times higher than in the bioremediation and electrokinetic groups, respectively. This indicates that the electric field promoted the conversion of TOC to DOC, which was assimilated by microorganisms to generate MBC. The analysis of the structure and functional genes of soil microbial communities revealed that the electric field accelerated the degradation of key petroleum hydrocarbon components and the soil carbon cycle. However, degradation rates varied, and MBC and DOC levels declined in later stages, indicating discontinuous pollutant degradation. The analysis of the ratio of organic carbon derived from petroleum and soil sources (<sup>12</sup>Cp/<sup>12</sup>Cs) revealed intermittent use of petroleum-derived carbon, potentially limiting the sustainable degradation of pollutants. The research results provide insights for improving BIO-EK remediation efficiency.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"217 ","pages":"Article 109665"},"PeriodicalIF":3.7,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143422731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A model based approach for monitoring Bordetella pertussis fermentation with an inline spectro-fluorescence probe

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-02-13 DOI: 10.1016/j.bej.2025.109674

Abhishek Mishra , Michael Vitelli , Boris Tartakovsky , Ibrahim M. Tamer , Hector Budman

Presently, the extraction of the antigen pertactin poses a challenge in the manufacturing of the whooping cough vaccine due to its low and variable yield [1]. In this work, a hybrid model that combines empirical and mechanistic parts and in-line fluorescence measurements is used to design an estimator for monitoring the manufacturing process in bioreactors. The empirical part of the hybrid model uses Partial Least Squares (PLS) regression to estimate biomass, carbon source, and pertactin productivity from fluorescence data. In view that significant correlations are observed between oxidative stress and productivity, the mechanistic part of the hybrid model is based on key oxidative reaction pathways. Estimation based on a hybrid model is shown to improve the prediction accuracy of antigen productivity as compared to purely empirical or purely mechanistic model-based estimators. The proposed estimator enables real-time monitoring of the manufacturing process and opens the possibility of future implementation of mid-point corrective actions.

{"title":"A model based approach for monitoring Bordetella pertussis fermentation with an inline spectro-fluorescence probe","authors":"Abhishek Mishra , Michael Vitelli , Boris Tartakovsky , Ibrahim M. Tamer , Hector Budman","doi":"10.1016/j.bej.2025.109674","DOIUrl":"10.1016/j.bej.2025.109674","url":null,"abstract":"<div><div>Presently, the extraction of the antigen pertactin poses a challenge in the manufacturing of the whooping cough vaccine due to its low and variable yield [1]. In this work, a hybrid model that combines empirical and mechanistic parts and in-line fluorescence measurements is used to design an estimator for monitoring the manufacturing process in bioreactors. The empirical part of the hybrid model uses Partial Least Squares (PLS) regression to estimate biomass, carbon source, and pertactin productivity from fluorescence data. In view that significant correlations are observed between oxidative stress and productivity, the mechanistic part of the hybrid model is based on key oxidative reaction pathways. Estimation based on a hybrid model is shown to improve the prediction accuracy of antigen productivity as compared to purely empirical or purely mechanistic model-based estimators. The proposed estimator enables real-time monitoring of the manufacturing process and opens the possibility of future implementation of mid-point corrective actions.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"217 ","pages":"Article 109674"},"PeriodicalIF":3.7,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143430121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimizing bamboo biomass for sustainable isobutanol production using genetically engineered Escherichia coli

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-02-11 DOI: 10.1016/j.bej.2025.109669

Said Nawab , Zahoor , Syed Bilal Shah , Mujeeb Ur Rahman , Hareef Ahmed Keerio , Inamur Rahman

Researchers are finding sustainable fuel alternatives due to the growing costs and environmental issues related to fossil energy. Isobutanol is an attractive biofuel with a higher energy content than ethanol and the potential to replace gasoline. Bamboo emerges as a promising and cost-effective feedstock for isobutanol production due to its fast growth and high holocellulosic content. By using response surface methodology, this study optimized glucose recovery from bamboo biomass by varying pretreatment temperature (30–70°C), reaction time (0.5–6 h), and NaOH concentration (0.5–3 %). The maximum glucose recovery was achieved using 1 % NaOH at 68°C for 6 hours, resulting in improved cellulose and reduced hemicellulose and lignin content in bamboo. This process released 31.01 mg/mL glucose, representing 65 % of available sugars. Structural modifications of the untreated and alkali-treated bamboo biomass were confirmed through Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). Isobutanol fermentation with the engineered E. coli strain JCL260 produced 2.28 g/L of isobutanol from enzymatic hydrolysate, achieving 22.86 % of the theoretical maximum. This study determines that cellulose from bamboo has been efficiently transformed into isobutanol through fermentation, although at a lower concentration associated with pure glucose.

{"title":"Optimizing bamboo biomass for sustainable isobutanol production using genetically engineered Escherichia coli","authors":"Said Nawab , Zahoor , Syed Bilal Shah , Mujeeb Ur Rahman , Hareef Ahmed Keerio , Inamur Rahman","doi":"10.1016/j.bej.2025.109669","DOIUrl":"10.1016/j.bej.2025.109669","url":null,"abstract":"<div><div>Researchers are finding sustainable fuel alternatives due to the growing costs and environmental issues related to fossil energy. Isobutanol is an attractive biofuel with a higher energy content than ethanol and the potential to replace gasoline. Bamboo emerges as a promising and cost-effective feedstock for isobutanol production due to its fast growth and high holocellulosic content. By using response surface methodology, this study optimized glucose recovery from bamboo biomass by varying pretreatment temperature (30–70°C), reaction time (0.5–6 h), and NaOH concentration (0.5–3 %). The maximum glucose recovery was achieved using 1 % NaOH at 68°C for 6 hours, resulting in improved cellulose and reduced hemicellulose and lignin content in bamboo. This process released 31.01 mg/mL glucose, representing 65 % of available sugars. Structural modifications of the untreated and alkali-treated bamboo biomass were confirmed through Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). Isobutanol fermentation with the engineered <em>E. coli</em> strain JCL260 produced 2.28 g/L of isobutanol from enzymatic hydrolysate, achieving 22.86 % of the theoretical maximum. This study determines that cellulose from bamboo has been efficiently transformed into isobutanol through fermentation, although at a lower concentration associated with pure glucose.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"217 ","pages":"Article 109669"},"PeriodicalIF":3.7,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143422730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation and study of CDs-WO3 composites with enhanced photocatalytic antimicrobial properties and degradation of dyes

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-02-11 DOI: 10.1016/j.bej.2025.109670

Qing Wang , Guanbin Wen , Zhengfang Yang , Qianqian Guo , Bin Zhang , Yanli Nie , Dongquan Wang

In this study, carbon dots (CDs) and tungsten trioxide (WO₃) were combined to prepare CDs-WO₃ composites for photocatalytic treatment of bacteria and organic dyes in wastewater. The optical properties, antimicrobial properties, antimicrobial mechanism, and photocatalytic degradation performance were also investigated. The CDs-WO₃ composites exhibited enhanced UV absorption intensity, broadened visible light absorption range, good charge transfer and carrier separation abilities, and reduced bandgap. The antimicrobial rate of CDs-WO₃ against Staphylococcus aureus can reach 98 %. The antibacterial mechanism of CDs-WO₃ was found to be the reactive oxygen species damage mechanism. Electron paramagnetic resonance (EPR) analysis revealed that the reactive oxygen species signal intensity of CDs-WO₃ was significantly higher than that of WO₃, indicating that more ·O₂^- and ·OH were produced. The analysis of bacterial cell activity demonstrated that CDs-WO₃ composites reduced the activity of respiratory chain dehydrogenase in bacteria, which resulted in increased lipid peroxidation in the cell membrane. The photocatalytic degradation performance showed that the photocatalytic degradation efficiency of CDs-WO₃ for methylene blue and malachite green reached 87 % and 88.04 %, respectively. The application of CDs-WO₃ in real water bodies also showed good antibacterial effect and dye degradation.

{"title":"Preparation and study of CDs-WO3 composites with enhanced photocatalytic antimicrobial properties and degradation of dyes","authors":"Qing Wang , Guanbin Wen , Zhengfang Yang , Qianqian Guo , Bin Zhang , Yanli Nie , Dongquan Wang","doi":"10.1016/j.bej.2025.109670","DOIUrl":"10.1016/j.bej.2025.109670","url":null,"abstract":"<div><div>In this study, carbon dots (CDs) and tungsten trioxide (WO<sub>3</sub>) were combined to prepare CDs-WO<sub>3</sub> composites for photocatalytic treatment of bacteria and organic dyes in wastewater. The optical properties, antimicrobial properties, antimicrobial mechanism, and photocatalytic degradation performance were also investigated. The CDs-WO<sub>3</sub> composites exhibited enhanced UV absorption intensity, broadened visible light absorption range, good charge transfer and carrier separation abilities, and reduced bandgap. The antimicrobial rate of CDs-WO<sub>3</sub> against <em>Staphylococcus aureus</em> can reach 98 %. The antibacterial mechanism of CDs-WO<sub>3</sub> was found to be the reactive oxygen species damage mechanism. Electron paramagnetic resonance (EPR) analysis revealed that the reactive oxygen species signal intensity of CDs-WO<sub>3</sub> was significantly higher than that of WO<sub>3</sub>, indicating that more <strong>·</strong>O<sub>2</sub><sup><strong>-</strong></sup> and <strong>·</strong>OH were produced. The analysis of bacterial cell activity demonstrated that CDs-WO<sub>3</sub> composites reduced the activity of respiratory chain dehydrogenase in bacteria, which resulted in increased lipid peroxidation in the cell membrane. The photocatalytic degradation performance showed that the photocatalytic degradation efficiency of CDs-WO<sub>3</sub> for methylene blue and malachite green reached 87 % and 88.04 %, respectively. The application of CDs-WO<sub>3</sub> in real water bodies also showed good antibacterial effect and dye degradation.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"217 ","pages":"Article 109670"},"PeriodicalIF":3.7,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143445402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Research on the removal of fluoride from low-concentration fluorine-containing industrial wastewater using adsorption methods

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-02-10 DOI: 10.1016/j.bej.2025.109668

Benfu Luo , Yuhang Liu , Yujing Yan , Haixing He , Jie Yu , Qiang Chen

To address the need for deep fluoride removal of low-concentration fluorine-containing industrial wastewater (2–10 mg/L) to below 1.5 mg/L in centralized industrial wastewater treatment plants, a study was conducted using adsorption methods. The adsorption performance and influencing factors of four adsorbent materials—active alumina (AA), hydroxyapatite (HAP), fly ash (FA), and bone char (BC)—were investigated. The results indicate that positioning the adsorption fluoride removal filter at the tail end of the industrial wastewater treatment process yields a fluoride removal efficiency that is over 10 % higher compared to placing it at the front end. The investigation into the adsorption effectiveness of the four adsorbents revealed that HAP dosage only needs 2 g to reduce 250 ml fluorinated industrial wastewater containing 2–10 mg/L to the target concentration of 1.5 mg/L or less. Moreover, the adsorption process requires only 30 minutes. From the perspective of adsorption performance, HAP is the most effective, followed by AA. However, considering the cost of these adsorbents, AA is more suitable as an adsorbent for treating low-concentration fluorine-containing wastewater. The findings of this study can serve as a valuable reference for the process design of deep adsorption fluoride removal in projects involving fluorine-containing industrial wastewater.

{"title":"Research on the removal of fluoride from low-concentration fluorine-containing industrial wastewater using adsorption methods","authors":"Benfu Luo , Yuhang Liu , Yujing Yan , Haixing He , Jie Yu , Qiang Chen","doi":"10.1016/j.bej.2025.109668","DOIUrl":"10.1016/j.bej.2025.109668","url":null,"abstract":"<div><div>To address the need for deep fluoride removal of low-concentration fluorine-containing industrial wastewater (2–10 mg/L) to below 1.5 mg/L in centralized industrial wastewater treatment plants, a study was conducted using adsorption methods. The adsorption performance and influencing factors of four adsorbent materials—active alumina (AA), hydroxyapatite (HAP), fly ash (FA), and bone char (BC)—were investigated. The results indicate that positioning the adsorption fluoride removal filter at the tail end of the industrial wastewater treatment process yields a fluoride removal efficiency that is over 10 % higher compared to placing it at the front end. The investigation into the adsorption effectiveness of the four adsorbents revealed that HAP dosage only needs 2 g to reduce 250 ml fluorinated industrial wastewater containing 2–10 mg/L to the target concentration of 1.5 mg/L or less. Moreover, the adsorption process requires only 30 minutes. From the perspective of adsorption performance, HAP is the most effective, followed by AA. However, considering the cost of these adsorbents, AA is more suitable as an adsorbent for treating low-concentration fluorine-containing wastewater. The findings of this study can serve as a valuable reference for the process design of deep adsorption fluoride removal in projects involving fluorine-containing industrial wastewater.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"216 ","pages":"Article 109668"},"PeriodicalIF":3.7,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143395350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced degradation of petroleum hydrocarbons by immobilizing Acinetobacter

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-02-07 DOI: 10.1016/j.bej.2025.109666

Jienan Li , Hua Zhang , Kuixi Mei , Leni Sun , Li Wang , Changhai Liang

The highly efficient elimination of petroleum pollution is essential for addressing environmental issues and social sustainability. A hydrocarbon-degrading bacterium SHC is isolated from petroleum-contaminated soil in Chengdu, China, to augment bioremediation efforts against crude oil contamination. According to the analysis of 16S rDNA sequences, strain SHC was identified as Acinetobacter. The degradation rate of 2 g/L crude oil by free bacterium reached 30 % within 7 days. Through the analysis of hydrocarbon biodegradation, strain SHC was found to biodegrade more long-chain n-alkanes (C19–C29). Further, we delve into utilizing agar as a microbial immobilization matrix, supplemented with nutrient salts and oil-absorbing materials, to foster the growth and activity of the bacterium in challenging environments characterized by high salinity and acidity. In the final immobilization bacterium, the degradation rate of 3 % salt concentration reaches 22 %, the degradation rate of 5 g/L crude oil is 45 %, and the degradation rate at a pH of 10 is 74 %, and was 59 % more effective than the free bacterium on the removal of crude oil under the same conditions. These findings strongly indicate that encapsulating Acinetobacter SHC can protect them against extreme conditions, bolstering bioremediation endeavors.

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引用次数: 0

Advancements of the Vibrio coralliilyticus eDNA detection based on Co-Fe PBA-assisted biosensors for the rapid coral-disease warning

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-02-06 DOI: 10.1016/j.bej.2025.109662

Yibo Zhang , Hongjie Liu , Shaopeng Wang , Hao Fu , Yuanyu Xie , Chaoxin Zhang , Man Zhang , Jie Lu , Liwei Wang , Kefu Yu

Vibrio coralliilyticus (V. coralliilyticus) can lead to severe coral diseases and is one of the main causes of coral bleaching. However, there is still a lack of convenient, and non-invasive detection methods for V. coralliilyticus, which brings inconvenience to coral health management. Here, a portable electrochemical biosensor was developed based on environmental DNA (eDNA) and cobalt-iron Prussian Blue Analogue (Co-Fe PBA) electrode material for detecting V. coralliilyticus. Notably, this sensor identified and quantified V. coralliilyticus eDNA in water through specific DNA probes, overcoming the previously reported complex and destructive pretreatment processes. Additionally, thanks to the high specific surface area of Co-Fe PBA, which provided abundant sites for probe anchoring, the constructed portable biosensor exhibited excellent detection performances. Specifically, the detection limit was as low as 19.0 fM, and the linear range was from 100 fM to 100 nM. In subsequent coral infection experiments, the eDNA of V. coralliilyticus increased on the eve of coral bleaching. Based on this change, the occurrence of coral bleaching was successfully predicted. Therefore, this portable biosensor is suitable for dynamic monitoring of V. coralliilyticus, helping to reveal the potential risks to coral health and providing a reference for ecological decision-making for coral reef protection.

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