The art of designed coiled-coils for the regulation of mammalian cells

IF 6.6 1区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Cell Chemical Biology Pub Date : 2024-08-15 DOI:10.1016/j.chembiol.2024.06.001

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Abstract

Synthetic biology aims to engineer complex biological systems using modular elements, with coiled-coil (CC) dimer-forming modules are emerging as highly useful building blocks in the regulation of protein assemblies and biological processes. Those small modules facilitate highly specific and orthogonal protein-protein interactions, offering versatility for the regulation of diverse biological functions. Additionally, their design rules enable precise control and tunability over these interactions, which are crucial for specific applications. Recent advancements showcase their potential for use in innovative therapeutic interventions and biomedical applications. In this review, we discuss the potential of CCs, exploring their diverse applications in mammalian cells, such as synthetic biological circuit design, transcriptional and allosteric regulation, cellular assemblies, chimeric antigen receptor (CAR) T cell regulation, and genome editing and their role in advancing the understanding and regulation of cellular processes.

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设计用于调节哺乳动物细胞的盘绕线圈的艺术。

合成生物学旨在利用模块化元素设计复杂的生物系统，而形成盘绕线圈（CC）二聚体的模块正在成为调控蛋白质组装和生物过程的非常有用的构件。这些小模块可促进蛋白质与蛋白质之间高度特异和正交的相互作用，为调控各种生物功能提供了多功能性。此外，它们的设计规则能够实现对这些相互作用的精确控制和可调谐性，这对特定应用至关重要。最近的进展展示了它们在创新治疗干预和生物医学应用方面的潜力。在这篇综述中，我们将讨论 CCs 的潜力，探讨它们在哺乳动物细胞中的各种应用，如合成生物电路设计、转录和异生调控、细胞组装、嵌合抗原受体 (CAR) T 细胞调控和基因组编辑，以及它们在促进对细胞过程的理解和调控方面的作用。

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来源期刊

Cell Chemical Biology Biochemistry, Genetics and Molecular Biology-Molecular Medicine

CiteScore

14.70

自引率

2.30%

发文量

143

期刊介绍： Cell Chemical Biology, a Cell Press journal established in 1994 as Chemistry & Biology, focuses on publishing crucial advances in chemical biology research with broad appeal to our diverse community, spanning basic scientists to clinicians. Pioneering investigations at the chemistry-biology interface, the journal fosters collaboration between these disciplines. We encourage submissions providing significant conceptual advancements of broad interest across chemical, biological, clinical, and related fields. Particularly sought are articles utilizing chemical tools to perturb, visualize, and measure biological systems, offering unique insights into molecular mechanisms, disease biology, and therapeutics.