Biofunctional photoelectrochemical/electrochemical immunosensor based on BiVO4/BiOI-MWCNTs and Au@PdPt for alpha-fetoprotein detection

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Bioelectrochemistry Pub Date : 2024-07-03 DOI:10.1016/j.bioelechem.2024.108773

Haotian Wu , Xiaozhan Yang

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Abstract

A biofunctional immunosensor combining photoelectrochemical (PEC) and electrochemical (EC) was proposed for the quantitative detection of the liver cancer marker alpha-fetoprotein (AFP) in human blood. BiVO₄/BiOI-MWCNTs photoactive materials were first prepared on conductive glass FTO, and the photoelectrode was functionalized by chitosan and glutaraldehyde. Then, the AFP capture antibody (Ab1) was successfully modified on the photoelectrode, and the label-free rapid detection of AFP antigen was achieved by PEC. In addition, Au@PdPt nanospheres were also used as a marker for binding to AFP detection antibody (Ab2). Due to the excellent catalytic properties of Au@PdPt in EC reaction, a signal increase in the EC response can be achieved when Ab2 binds to the AFP antigen, which ensures high sensitivity for the detection of AFP. The detection limits of PEC and EC are 0.050 pg/mL and 0.014 pg/mL, respectively. The sensor also possesses good specificity, stability and reproducibility, shows excellent performance in the detection of clinical samples and has good clinical applicability.

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基于 BiVO4/BiOI-MWCNTs 和 Au@PdPt 的生物功能光电化学/电化学免疫传感器，用于检测甲胎蛋白。

研究人员提出了一种光电化学（PEC）和电化学（EC）相结合的生物功能免疫传感器，用于定量检测人体血液中的肝癌标志物甲胎蛋白（AFP）。首先在导电玻璃 FTO 上制备了 BiVO4/BiOI-MWCNTs 光活性材料，并用壳聚糖和戊二醛对光电极进行了功能化。随后，AFP 捕获抗体（Ab1）被成功修饰在光电极上，并通过 PEC 实现了对 AFP 抗原的无标记快速检测。此外，Au@PdPt 纳米球还被用作与 AFP 检测抗体（Ab2）结合的标记物。由于 Au@PdPt 在 EC 反应中具有良好的催化特性，当 Ab2 与 AFP 抗原结合时，EC 反应的信号会增加，从而确保了 AFP 检测的高灵敏度。PEC 和 EC 的检测限分别为 0.050 pg/mL 和 0.014 pg/mL。该传感器还具有良好的特异性、稳定性和重现性，在检测临床样本时表现出优异的性能，具有良好的临床适用性。

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来源期刊

Bioelectrochemistry 生物-电化学

CiteScore

9.10

自引率

6.00%

发文量

238

审稿时长

38 days

期刊介绍： An International Journal Devoted to Electrochemical Aspects of Biology and Biological Aspects of Electrochemistry Bioelectrochemistry is an international journal devoted to electrochemical principles in biology and biological aspects of electrochemistry. It publishes experimental and theoretical papers dealing with the electrochemical aspects of: • Electrified interfaces (electric double layers, adsorption, electron transfer, protein electrochemistry, basic principles of biosensors, biosensor interfaces and bio-nanosensor design and construction. • Electric and magnetic field effects (field-dependent processes, field interactions with molecules, intramolecular field effects, sensory systems for electric and magnetic fields, molecular and cellular mechanisms) • Bioenergetics and signal transduction (energy conversion, photosynthetic and visual membranes) • Biomembranes and model membranes (thermodynamics and mechanics, membrane transport, electroporation, fusion and insertion) • Electrochemical applications in medicine and biotechnology (drug delivery and gene transfer to cells and tissues, iontophoresis, skin electroporation, injury and repair). • Organization and use of arrays in-vitro and in-vivo, including as part of feedback control. • Electrochemical interrogation of biofilms as generated by microorganisms and tissue reaction associated with medical implants.