Bioelectrochemistry最新文献

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Corrigendum to "Molecular monolayers on silicon as substrates for biosensors" [Bioelectrochem. 80(1) (2010) 17-25]. 硅上的分子单层作为生物传感器的基底"[Bioelectrochem.

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2024-11-17 DOI: 10.1016/j.bioelechem.2024.108849

L Touahir, P Allongue, D Aureau, R Boukherroub, J-N Chazalviel, E Galopin, A C Gouget-Laemmel, C Henry de Villeneuve, A Moraillon, J Niedziółka-Jönsson, F Ozanam, J Salvador Andresa, S Sam, I Solomon, S Szunerits

引用次数: 0

Label-free electrochemical biosensor with magnetic self-assembly constructed via PNA-DNA hybridization process on α-Fe2O3/Fe3O4 nanosheets for APOE ε4 genes ultrasensitive detection 通过 PNA-DNA 杂交过程在 α-Fe2O3/Fe3O4 纳米片上构建的磁性自组装无标记电化学生物传感器，用于 APOE ε4 基因的超灵敏检测

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2024-11-15 DOI: 10.1016/j.bioelechem.2024.108847

Zhihao Xu , Zhixiang Lv , Huijiao Yang , Jiashuo Zhang , Zijie Sun , Dawei He , Ruijiang Liu

A label-free electrochemical DNA detection strategy based on self-assembled α-Fe₂O₃/Fe₃O₄ nanosheets with PNA-DNA hybridization process was developed for ultrasensitive detection of APOE ε4 gene, one of the most robust genetic risks for Alzheimer’s Disease (AD). In this work, magnetic α-Fe₂O₃/Fe₃O₄ heterogeneous nanosheets were prepared by hydrothermal-calcined reduction method and loaded with Au nanoparticles (AuNPs) on their surfaces. The magnetic α-Fe₂O₃/Fe₃O₄@Au nanocomposites significantly enhanced the electrochemical response as a signal amplification matrix and were able to bind to the magnetic glassy carbon electrode (MGCE) surface by magnetic self-assembly. Moreover, owing to the high specificity and stable binding capacity of PNA with respect to the target DNA, the biosensor not only enabled accurate (the limit of detection was estimated to be 0.147 pM) and rapid detection of the APOE ε4 gene, but also exhibited excellent specificity, stability and regeneration capability. Additional, the satisfactory recoveries were also obtained in real samples of human serum, ranging from 92.83 % to 106.22 % with relative standard deviation (RSD) between 0.25 % and 1.85 %. The results possessed important reference value for exploring the application of DNA biosensor technology in the diagnosis of APOE gene mutation.

研究人员开发了一种基于自组装α-Fe2O3/Fe3O4纳米片与PNA-DNA杂交过程的无标记DNA电化学检测策略，用于超灵敏检测APOE ε4基因，该基因是阿尔茨海默病（AD）最稳健的遗传风险之一。本研究采用水热煅烧还原法制备了磁性α-Fe2O3/Fe3O4异质纳米片，并在其表面负载了金纳米粒子（AuNPs）。磁性α-Fe2O3/Fe3O4@Au纳米复合材料作为信号放大基质显著增强了电化学响应，并能通过磁性自组装结合到磁性玻璃碳电极（MGCE）表面。此外，由于 PNA 与目标 DNA 具有高度的特异性和稳定的结合能力，该生物传感器不仅能准确（检测限估计为 0.147 pM）、快速地检测 APOE ε4 基因，而且表现出卓越的特异性、稳定性和再生能力。此外，该方法在实际人体血清样品中也获得了令人满意的回收率，回收率在 92.83 % 至 106.22 % 之间，相对标准偏差（RSD）在 0.25 % 至 1.85 % 之间。这些结果对探索 DNA 生物传感器技术在 APOE 基因突变诊断中的应用具有重要的参考价值。

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引用次数: 0

Time-resolved electromechanical and conductive behavior of nanostructured bilayers tethered to the surface of the electrode with incorporated channel proteins and peptides. 用通道蛋白和肽将纳米结构双分子层系在电极表面的时间分辨机电和导电行为。

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2024-11-15 DOI: 10.1016/j.bioelechem.2024.108848

Aleksandra Stefanowska, Michał Czapczyński, Piotr Koprowski, Adam Szewczyk, Paweł Krysiński

The influence of incorporation of mitochondrial inner membrane potassium channel, and channel-forming peptide - Gramicidin on the ion transport and electromechanical properties of model lipid membranes tethered to gold electrode was electrochemically investigated by chronoamperometric and impedance spectroscopy techniques. In the case of the potassium channel the ion transport properties were modulated with channel-specific inhibitor - ATP-Mg²⁺ complex, whereas in the case of gramicidin peptide - by replacing potassium with sodium ions. The observed two exponential current-time responses of the systems studied were interpreted in terms of ion penetration and electrostriction of tethered lipid bilayer membrane, and conclusions supported with the experiments on alkanethiol self-assembled monolayers of different alkanethiol chain lengths deposited on gold.

通过计时器和阻抗光谱技术，电化学研究了线粒体内膜钾通道和通道形成肽--苎麻素对系在金电极上的模型脂膜的离子传输和机电特性的影响。在钾通道的情况下，使用通道特异性抑制剂--ATP-Mg2+ 复合物来调节离子传输特性；而在gramicidin 肽的情况下，则使用钠离子取代钾离子。观察到的所研究系统的两个指数电流-时间反应被解释为离子渗透和系留脂质双分子层膜的电致伸缩，结论得到了沉积在金上的不同烷硫醇链长度的烷硫醇自组装单层实验的支持。

引用次数: 0

Sensitive detection of K-ras gene by a dual-mode “on-off-on” sensor based on bipyridine ruthenium-MOF and bis-enzymatic cleavage technology 基于联吡啶钌-MOF 和双酶裂解技术的 "开-关-开 "双模式传感器对 K-ras 基因的灵敏检测。

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2024-11-05 DOI: 10.1016/j.bioelechem.2024.108845

Haotian Xie , Zhaojiang Yin , Guobin Wei , Binghui Li , Hanfeng Cui , Hao Fan , Jing Zhang

This study developed a dual-mode “on-off-on” sensor based on a bipyridine ruthenium metal–organic framework (Ru-MOF) and dual enzyme cleavage technology for the sensitive detection of the K-ras gene. The sensor combines electrogenerated chemiluminescence (ECL) and fluorescence (FL) detection modes, achieving high sensitivity and specificity in detecting the K-ras gene through catalytic hairpin assembly (CHA) and dual enzyme cleavage reactions. Experimental results showed that the detection limits for the K-ras gene were 0.044 fM (ECL) and 0.16 fM (FL), demonstrating excellent selectivity and stability during detection. Through testing actual samples, the sensor has shown potential for application in complex biological environments. This method offers an efficient and reliable new tool for cancer diagnosis and treatment.

该研究开发了一种基于双吡啶钌金属有机框架（Ru-MOF）和双酶裂解技术的双模式 "开-关-开 "传感器，用于灵敏检测 K-ras 基因。该传感器结合了电致化学发光（ECL）和荧光（FL）检测模式，通过催化发夹组装（CHA）和双酶裂解反应实现了对 K-ras 基因的高灵敏度和特异性检测。实验结果表明，该传感器对 K-ras 基因的检测限分别为 0.044 fM（ECL）和 0.16 fM（FL），具有极佳的选择性和检测稳定性。通过测试实际样品，该传感器显示出在复杂生物环境中的应用潜力。这种方法为癌症诊断和治疗提供了一种高效可靠的新工具。

引用次数: 0

A novel label-free impedance biosensor for KRAS G12C mutations detection based on PET-RAFT and ROP synergistic signal amplification 基于 PET-RAFT 和 ROP 协同信号放大技术的新型无标记阻抗生物传感器，用于检测 KRAS G12C 突变。

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2024-11-02 DOI: 10.1016/j.bioelechem.2024.108844

Yaping Zhang , Haiyan Wei , Liang Guo , Wei Gao , Di Cheng , Yanju Liu

The KRAS G12C mutations, as crucial biomarkers, are closely associated with non-small cell lung cancer. Here, a novel label-free electrochemical biosensor with synergistic signal amplification of photocell energy transfer-reversible addition fragmentation chain transfer (PET-RAFT) and ring-opening polymerization (ROP) was developed for the first time for sensitive detection of KRAS G12C mutations. Specifically, hairpin DNA (hDNA), which act as biomolecular probe, was self-assembled on Au electrode surface by Au-S bond. 4-cyano-4-[(dodecylsulfanylthiocarbonyl) sulfanyl] pentanoic acid (CDTPA), the chain transfer agent of PET-RAFT reaction, was then attached to hDNA via amide bond. After that, the target DNA (tDNA) was captured on the electrode surface by complementary base pairing with hDNA. Subsequently, large numbers of electro-active monomers N-acryloxysuccinimide (NAS) were successfully grafted to the electrode surface via PET-RAFT reaction, which provided plenty of junction sites for doxorubicin-polycaprolactone (Dox-PCL) synthesized by ROP. Finally, the Dox-PCL was connected to the electrode surface by ester bond, significantly amplifying the electrochemical signal. Under optimized conditions, the biosensor has a wide linear detection range of 0.1 pM to 1 μM, with a detection limit of 86.9 fM. Attribute to its high sensitivity, specificity, reproducibility and stability, this biosensor possesses considerable potential in early diagnosis of disease and biomedical research.

KRAS G12C 突变作为重要的生物标志物，与非小细胞肺癌密切相关。本文首次开发了一种新型无标记电化学生物传感器，利用光电池能量转移-可逆加成碎片链转移（PET-RAFT）和开环聚合（ROP）的协同信号放大作用灵敏检测 KRAS G12C 突变。具体来说，发夹 DNA（hDNA）作为生物分子探针，通过 Au-S 键在金电极表面自组装。然后，PET-RAFT 反应的链转移剂 4-氰基-4-[（十二烷基硫代羰基）硫基]戊酸（CDTPA）通过酰胺键连接到 hDNA 上。然后，目标 DNA（tDNA）通过与 hDNA 的碱基互补配对被捕获到电极表面。随后，大量电活性单体 N-acryloxysuccinimide (NAS) 通过 PET-RAFT 反应成功接枝到电极表面，为 ROP 合成的多柔比星-聚己内酯（Dox-PCL）提供了大量接合位点。最后，Dox-PCL 通过酯键连接到电极表面，从而显著放大了电化学信号。在优化条件下，该生物传感器的线性检测范围为 0.1 pM 至 1 μM，检测限为 86.9 fM。该生物传感器具有灵敏度高、特异性强、重现性好和稳定性高等特点，在疾病早期诊断和生物医学研究方面具有相当大的潜力。

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引用次数: 0

Corrigendum to "Analysis of electromagnetic response of cells and lipid membranes using a model-free method" [Bioelectrochemistry 152 (2023) 108444]. 对 "利用无模型方法分析细胞和脂膜的电磁响应"[生物电化学 152 (2023) 108444] 的更正。

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2024-11-02 DOI: 10.1016/j.bioelechem.2024.108841

Yingxian Lu, Xiaping Tang, Yanyu Zhao, Tianyu Jiang, Jiayao Zhou, Xiaofei Wang, Bing Huang, Lingyu Liu, Hu Deng, Yujing Huang, Yigong Shi

引用次数: 0

Advanced laser-induced graphene-based electrochemical immunosensor for the detection of C-reactive protein 基于石墨烯的先进激光诱导电化学免疫传感器，用于检测 C 反应蛋白。

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2024-10-28 DOI: 10.1016/j.bioelechem.2024.108842

Sri Ramulu Torati , Gymama Slaughter

C-reactive protein (CRP) is a critical biomarker for detecting inflammation and forecasting cardiovascular disease. We present an advanced electrochemical immunosensor utilizing laser-induced graphene (LIG)/MXene-gold nanoparticles (Mx-AuNPs) electrode for CRP detection. The Mx-AuNPs nanocomposite, synthesized via in-situ reduction of HAuCl₄ by MXene, leverages MXene’s reducing properties for effective nanoparticle deposition, confirmed through scanning electron microscopy. This electrode demonstrates superior electrochemical performance due to enhanced surface area and synergy between LIG and Mx-AuNPs, improving overall electrode conductivity. The A-CRP antibody, immobilized via a cysteamine linker, enables CRP detection. The immunosensor achieves excellent detection across 10 pg mL⁻¹ to 10 µg mL⁻¹ CRP, with a low detection limit of 1.45 pg mL⁻¹, and shows high selectivity for CRP. This LIG/Mx-AuNPs-based immunosensor is promising for sensitive CRP detection, aiding early cardiovascular disease diagnosis and improving patient outcomes.

C 反应蛋白 (CRP) 是检测炎症和预测心血管疾病的重要生物标志物。我们介绍了一种利用激光诱导石墨烯（LIG）/MXene-金纳米颗粒（Mx-AuNPs）电极检测 CRP 的先进电化学免疫传感器。Mx-AuNPs 纳米复合材料是通过 MXene 原位还原 HAuCl4 合成的，它利用 MXene 的还原特性实现了纳米粒子的有效沉积，这一点已通过扫描电子显微镜得到证实。由于 LIG 和 Mx-AuNPs 的表面积增大、协同作用增强，这种电极显示出卓越的电化学性能，从而提高了电极的整体电导率。通过半胱胺连接体固定的 A-CRP 抗体可实现 CRP 检测。该免疫传感器对 10 pg mL-1 至 10 µg mL-1 的 CRP 具有出色的检测能力，检测限低至 1.45 pg mL-1，并对 CRP 具有高选择性。这种基于 LIG/Mx-AuNPs 的免疫传感器有望实现灵敏的 CRP 检测，帮助早期心血管疾病诊断并改善患者预后。

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引用次数: 0

Expanding the cell quantity of CRISPR/Cas9 gene editing by continuous microfluidic electroporation chip 利用连续微流控电穿孔芯片扩大 CRISPR/Cas9 基因编辑的细胞数量。

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2024-10-28 DOI: 10.1016/j.bioelechem.2024.108840

Zixi Li , Xinyue Su , Yihong Lin, Yu Zhang, Anlan Zhang, Xin Wu, Xi Jiyu, Qin Li, Zewen Wei

CRISPR/Cas9-mediated gene editing offers promising and safe therapeutic options for a wide range of diseases. The technical difficulty of efficiently acquiring large quantities of gene-edited therapeutic cells in a short time period is now preventing the widespread clinical application of CRISPR/Cas9-mediated gene editing. Herein, a Large Volume Continuous Electroporation Chip (LaViE-Chip) has been developed to address the challenge of acquiring sufficient quantities of genetically edited cells for CRISPR/Cas9 gene editing. By connecting multiple relatively narrow microfluidic channels in parallel, a satisfactory balance between cell flow volume and electric field uniformity was achieved with two simple off-chip electrodes, which also isolated harmful effects around electrodes from target cells. Meanwhile, by carefully designing the curvature of the microfluidic channel, hydrodynamic controlled rotation of target cells has been realized to improve the transfection efficiency and cell viability. With these improvements, the LaViE-Chip realized 71.06 % electrotransfection efficiency, 84.3 % cell viability, and 10⁷ cell/min cell processing speed. Moreover, the first successful incessant CRISPR gene editing by electroporation has been demonstrated, laying the technical foundation of therapeutic CRISPR gene editing.

CRISPR/Cas9 介导的基因编辑为多种疾病提供了安全可靠的治疗方案。目前，在短时间内高效获取大量基因编辑治疗细胞的技术难度阻碍了 CRISPR/Cas9 介导的基因编辑技术在临床上的广泛应用。在此，我们开发了一种大容量连续电穿孔芯片（LaViE-Chip），以解决为CRISPR/Cas9基因编辑获取足够数量基因编辑细胞的难题。通过平行连接多个相对较窄的微流体通道，利用两个简单的片外电极实现了细胞流动体积和电场均匀性之间令人满意的平衡，同时还隔离了电极周围对靶细胞的有害影响。同时，通过精心设计微流体通道的曲率，实现了靶细胞的流体力学可控旋转，提高了转染效率和细胞活力。通过这些改进，LaViE 芯片实现了 71.06% 的电转染效率、84.3% 的细胞存活率和 107 个细胞/分钟的细胞处理速度。此外，通过电穿孔首次成功实现了不间断的 CRISPR 基因编辑，为治疗性 CRISPR 基因编辑奠定了技术基础。

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引用次数: 0

A sensitive electrochemiluminescence immunosensor for CEA detection based on the ECL-RET between zinc-based metal–organic frameworks and ZiF-8@PDA 一种基于锌基金属有机框架和 ZiF-8@PDA 之间的 ECL-RET 的灵敏 CEA 检测电化学发光免疫传感器。

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2024-10-26 DOI: 10.1016/j.bioelechem.2024.108843

Yige Li, Yingying Cheng, Haoyi Ren, Tiantian Ji, Zhengyi Zhao, Hongling Li, Chenglin Hong

In this study, we developed a new system that using zinc-based metal–organic frameworks NH₂-Zn-PTC as the donor and ZiF-8@PDA as the acceptor to achieve highly sensitive detection of carcinoembryonic antigen (CEA), using the fundamentals of electrochemiluminescence resonance energy transfer (ECL-RET). Firstly, the aggregation-induced quenching effect (ACQ) was eliminated by the coordination of PTC in MOF and the ECL signal was improved. Secondly, the ECL signal was further amplified by using Au NPs and amino groups as co-reaction promoters to generate more SO₄^.−. In addition, the introduction of ZiF-8@PDA as an acceptor and NH₂-Zn-PTC as a donor took advantage of the feature of partial overlap of the UV–vis absorption spectrum and ECL emission spectra between the two, thereby effectively initiating the ECL-RET behavior, which improved the detection sensitivity of the sensor. The prepared immunosensor showed good linearity in the concentration range of 10⁻⁴ to 80 ng/mL with a detection limit of 18.20 fg/mL. This makes it promising for clinical testing of tumor markers.

本研究以锌基金属有机框架 NH2-Zn-PTC 为供体，ZiF-8@PDA 为受体，利用电化学发光共振能量转移（ECL-RET）的基本原理开发了一种新的系统，实现了对癌胚抗原（CEA）的高灵敏度检测。首先，PTC 在 MOF 中的配位消除了聚集诱导淬灭效应（ACQ），提高了 ECL 信号。其次，利用 Au NPs 和氨基作为共反应促进剂生成更多的 SO4.-，进一步放大了 ECL 信号。此外，引入 ZiF-8@PDA 作为受体，NH2-Zn-PTC 作为供体，利用了两者的紫外可见吸收光谱和 ECL 发射光谱部分重叠的特点，从而有效地启动了 ECL-RET 行为，提高了传感器的检测灵敏度。制备的免疫传感器在 10-4 至 80 ng/mL 的浓度范围内表现出良好的线性关系，检测限为 18.20 fg/mL。这使其有望用于肿瘤标志物的临床检测。

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引用次数: 0

Laccase mimetics as sensing elements for amperometric assay of 5-hydroxyindoleacetic acid in urine 将漆酶模拟物作为安培法测定尿液中 5-羟基吲哚乙酸的传感元件。

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2024-10-24 DOI: 10.1016/j.bioelechem.2024.108839

Olha Demkiv , Wojciech Nogala , Nataliya Stasyuk , Marcin Holdynski , Nina Dimcheva , Taras Danysh , Monika Asztemborska , Mykhailo Gonchar

Monitoring of the levels of 5-hydroxyindole-3-acetic acid (5-HIAA) is of significant importance for diagnostics of carcinoid tumors. We propose simple catalytic electrochemical sensors for the determination of 5-HIAA in urine using laccase and its mimetics. Laccase-like nanozymes (LacNZs) were synthesized via a chemical reduction, and resulting PtMn and MnO₂ nanoflowers (NFs) demonstrated laccase-like activity similar to the laccase from the Trametes zonata. In addition, these LacNZs showed enhanced stability under a wide range of pH (3.0–7.5), temperatures (4–70 °C), and ionic strengths (up to 500 mM NaCl). The developed PtMn NF/graphite electrode, similar to a laccase/graphite electrode, can detect 5-HIAA with a high sensitivity (25 000 ± 12 A·M⁻¹·m⁻² and 1900 ± 9 A·M⁻¹·m⁻², respectively) and have linear ranges of 0.3 – 15 μM and 2 – 50 μM. The sensors work at low working potentials with a detection limit of 0.16 and 1.4 μM, covering the normal and pathologic ranges of 5-HIAA (1 – 50 μM) content in urine. They have been successfully applied to 5-HIAA assay in urine samples of people with various diseases and revealed good recovery values and reproducibility. Additionally, the LacNZ-sensor has the best stability and can be used up to 20 days.

监测 5- 羟基吲哚-3-乙酸（5-HIAA）的水平对于类癌的诊断具有重要意义。我们提出了利用漆酶及其模拟物测定尿液中 5-HIAA 的简单催化电化学传感器。我们通过化学还原法合成了类似漆酶的纳米酶（LacNZs），所得到的铂锰和二氧化锰纳米花（NFs）表现出了类似漆酶的活性，其活性类似于来自瘤葡萄的漆酶。此外，这些 LacNZs 在广泛的 pH 值（3.0-7.5）、温度（4-70 °C）和离子强度（高达 500 mM NaCl）条件下显示出更强的稳定性。所开发的铂锰 NF/石墨电极类似于漆包线/石墨电极，可以高灵敏度（分别为 25 000 ± 12 A-M-1-m-2 和 1900 ± 9 A-M-1-m-2）检测 5-HIAA，线性范围分别为 0.3 - 15 μM 和 2 - 50 μM。传感器在低工作电位下工作，检测限分别为 0.16 和 1.4 μM，涵盖了尿液中 5-HIAA（1 - 50 μM）含量的正常和病理范围。它们已成功应用于各种疾病患者尿样中 5-HIAA 的检测，并显示出良好的回收率和重现性。此外，LacNZ 传感器的稳定性最好，可使用长达 20 天。

{"title":"Laccase mimetics as sensing elements for amperometric assay of 5-hydroxyindoleacetic acid in urine","authors":"Olha Demkiv , Wojciech Nogala , Nataliya Stasyuk , Marcin Holdynski , Nina Dimcheva , Taras Danysh , Monika Asztemborska , Mykhailo Gonchar","doi":"10.1016/j.bioelechem.2024.108839","DOIUrl":"10.1016/j.bioelechem.2024.108839","url":null,"abstract":"<div><div>Monitoring of the levels of 5-hydroxyindole-3-acetic acid (5-HIAA) is of significant importance for diagnostics of carcinoid tumors. We propose simple catalytic electrochemical sensors for the determination of 5-HIAA in urine using laccase and its mimetics. Laccase-like nanozymes (LacNZs) were synthesized <em>via</em> a chemical reduction, and resulting PtMn and MnO<sub>2</sub> nanoflowers (NFs) demonstrated laccase-like activity similar to the laccase from the <em>Trametes zonata</em>. In addition, these LacNZs showed enhanced stability under a wide range of pH (3.0–7.5), temperatures (4–70 °C), and ionic strengths (up to 500 mM NaCl). The developed PtMn NF/graphite electrode, similar to a laccase/graphite electrode, can detect 5-HIAA with a high sensitivity (25 000 ± 12 A·M<sup>−1</sup>·m<sup>−2</sup> and 1900 ± 9 A·M<sup>−1</sup>·m<sup>−2</sup>, respectively) and have linear ranges of 0.3 – 15 μM and 2 – 50 μM. The sensors work at low working potentials with a detection limit of 0.16 and 1.4 μM, covering the normal and pathologic ranges of 5-HIAA (1 – 50 μM) content in urine. They have been successfully applied to 5-HIAA assay in urine samples of people with various diseases and revealed good recovery values and reproducibility. Additionally, the LacNZ-sensor has the best stability and can be used up to 20 days.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"161 ","pages":"Article 108839"},"PeriodicalIF":4.8,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142542447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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