Bioelectrochemistry最新文献

Microalgae cells with extracellular gold nanoparticles for enhanced photobioelectrochemical activity 微藻细胞与细胞外金纳米粒子增强光生物电化学活性

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2026-10-01 Epub Date: 2026-03-04 DOI: 10.1016/j.bioelechem.2026.109264

Caio C.G. Silva , Alessandro Cacia , Hernán D. Rojas-Mantilla , Maria V.B. Zanoni , Saulo S. Garrido , Felipe Conzuelo

Photobioelectrochemical systems (PBEs) harness the machinery of photosynthetic microorganisms to convert solar energy into electricity. However, the inefficient electron transfer at the cell-electrode interface remains as the key performance limitation. Herein, we report a plasmonic biohybrid strategy to enhance extracellular electron transfer in Chlorella-based photobioelectrodes by integrating gold nanoparticles (AuNPs) localized on the microalgae membrane. Two approaches are investigated, consisting of physical mixing of isolated cells with AuNPs and cultivating microalgae in a growth medium supplemented with AuNPs, with the second approach allowing to obtain about significantly higher photocurrent responses. Optimized bioelectrodes yield photocurrent densities of up to 132 μA cm⁻², 74% higher than bioelectrodes fabricated using unmodified cells. Spectral response analysis reveals a strong resonance at 525 nm, consistent with the plasmonic properties of AuNPs. Importantly, pigment content, growth kinetics, and membrane integrity are preserved, confirming the biocompatibility of the modification. This work presents a facile and effective route to engineer photosynthetic bioelectrodes using nanomaterials, advancing the design of high-performance PBEs for solar-to-electricity conversion.

光生物电化学系统（PBEs）利用光合微生物的机制将太阳能转化为电能。然而，电池-电极界面的低效率电子转移仍然是主要的性能限制。在此，我们报道了一种等离子体生物杂交策略，通过整合定位在微藻膜上的金纳米颗粒（AuNPs）来增强小球藻基光生物电极的细胞外电子转移。研究人员研究了两种方法，一种是将分离细胞与AuNPs物理混合，另一种是在添加了AuNPs的生长培养基中培养微藻，第二种方法可以获得更高的光电流响应。优化后的生物电极产生的光电流密度高达132 μA cm−2，比使用未修饰的细胞制备的生物电极高74%。光谱响应分析显示在525 nm处有强烈的共振，与AuNPs的等离子体特性一致。重要的是，色素含量、生长动力学和膜完整性都得到了保存，证实了改性的生物相容性。这项工作提出了一种简单有效的途径来利用纳米材料设计光合生物电极，推进了高性能PBEs的设计，用于太阳能到电力的转换。

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引用次数: 0

Anthraquinone disulfonate as a stable redox mediator for efficient air-cathodes at neutral pH in dual-chamber microbial fuel cells 二磺酸蒽醌作为双室微生物燃料电池中性pH下高效空气阴极的氧化还原介质

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2026-10-01 Epub Date: 2026-03-09 DOI: 10.1016/j.bioelechem.2026.109279

Antoine Vautier, James A. Behan, Charlotte Bodin, Florence Geneste, Frédéric Barrière

Microbial Fuel Cells (MFCs) are commonly developed as organic-matter oxidizing bioanodes with abiotic air cathodes. However, O₂ reduction requires active aeration and/or the use of expensive catalysts using noble metals. In this study, 2,7-anthraquinone disulfonate (2,7-AQDS), an organic redox mediator commonly used in aqueous redox flow battery systems (AORFBs), served as a redox-stable intermediate for oxygen reduction. Dual-chamber MFC pilots were developed with 2,7-AQDS in the catholyte under both anoxic and aerobic conditions and compared to pilots with ferricyanide catholytes. In both conditions, cyclic voltammetry studies confirmed similar and efficient electroactivity despite the proximity AQDS formal redox potential to that of acetate oxidation. Mediated air-cathodes achieved open-circuit voltage (OCV) of 510 mV and current densities of 140 μA/cm², nearly double those of air-only cathodes (72 μA/cm²), while delivering a 33% higher power density (12 mW/m² vs. 8 mW/m²). Passive catholyte aeration enabled continuous reoxidation of reduced 2,7-AQDS at 8.8 × 10⁻⁸ mol/s, exceeding the AQDS reduction rate by the bioanode (2.5 × 10⁻¹⁰ mol/s), thus ensuring effective self-regeneration and stable AQDS concentration. These results demonstrate that AQDS coupled with passive oxygen supply sustains biofilm activity with enhances current and power, and allow long-term / low-maintenance MFC operation and organic-matter oxidation.

微生物燃料电池（mfc）通常被开发为有机物氧化生物阳极和非生物空气阴极。然而，O2还原需要主动曝气和/或使用昂贵的贵金属催化剂。在本研究中，2,7-蒽醌二磺酸盐（2,7- aqds）作为水氧化还原液流电池系统（AORFBs）中常用的有机氧化还原介质，作为氧还原的氧化还原稳定中间体。在缺氧和有氧条件下培养具有2,7- aqds的双室MFC飞行员，并与具有铁氰化阴极的飞行员进行比较。在这两种情况下，循环伏安法研究证实，尽管AQDS形式氧化还原电位与醋酸氧化电位接近，但电活性相似且有效。介质空气阴极的开路电压（OCV）为510 mV，电流密度为140 μA/cm2，几乎是纯空气阴极（72 μA/cm2）的两倍，而功率密度（12 mW/m2 vs. 8 mW/m2）提高了33%。被动阴极曝气使还原的2,7-AQDS以8.8 × 10−8 mol/s的速度连续再氧化，超过了生物阳极对AQDS的还原速率（2.5 × 10−10 mol/s），从而保证了有效的自再生和稳定的AQDS浓度。这些结果表明，AQDS与被动供氧相结合，可以在增强电流和功率的情况下维持生物膜的活性，并允许长期/低维护的MFC运行和有机物氧化。

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引用次数: 0

Developing fast scan cyclic voltammetry at carbon fiber microelectrodes to quantify short chain fatty acids in situ 碳纤维微电极快速扫描循环伏安法原位定量短链脂肪酸。

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2026-10-01 Epub Date: 2026-03-04 DOI: 10.1016/j.bioelechem.2026.109266

Terdha Narla , Uma Nudurupati, Yangguang Ou

Acetic, propionic, and butyric acids are short chain fatty acids (SCFAs) and the most abundant metabolites produced by gut bacteria. To uncover mechanistic insights of their function throughout the body, it is critical to measure rapid SCFA fluxes in sites of action such as brain, muscle, and skin. Current approaches have focused on fecal and plasma measurements, where SCFA levels are low and not representative of tissue-specific concentrations or fluxes. Thus, a rapid, in situ methodology is needed. Fast scan cyclic voltammetry (FSCV) at carbon fiber microelectrodes (CFMs) has the spatiotemporal resolution to fill this need. However, the electrochemical response of CFMs to SCFAs have not been explored until now. By coupling FSCV at CFMs with flow injection analysis of each SCFA, we demonstrate there are distinct peaks in the cyclic voltammograms for each fatty acid. Some of these peaks display faradaic behavior in scan rate, holding potential, and switching potential experiments. Interestingly, we identify several peaks that are concentration-sensitive and therefore are promising quantifiable markers of SCFA dynamics and fluxes. This work lays the foundation in understanding the response of CFMs to fatty acids and demonstrate the utility of FSCV at CFMs for the in situ quantitation of SCFAs.

乙酸、丙酸和丁酸是短链脂肪酸（SCFAs），是肠道细菌产生的最丰富的代谢物。为了揭示其在全身功能的机制，测量脑、肌肉和皮肤等作用部位的快速SCFA通量至关重要。目前的方法主要集中在粪便和血浆测量，其中SCFA水平较低，不能代表组织特异性浓度或通量。因此，需要一种快速的就地方法。碳纤维微电极（CFMs）的快速扫描循环伏安法（FSCV）具有满足这一需求的时空分辨率。然而，cfm对scfa的电化学响应目前还没有研究。通过将cfm的FSCV与每种SCFA的流动注射分析相结合，我们发现每种脂肪酸的循环伏安图中都有不同的峰。其中一些峰在扫描速率、保持电位和开关电位实验中显示出法拉第行为。有趣的是，我们发现了几个对浓度敏感的峰，因此是有希望的SCFA动态和通量的可量化标记。这项工作为理解cfm对脂肪酸的反应奠定了基础，并证明了FSCV在cfm中用于scfa原位定量的实用性。

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引用次数: 0

Nanotip acetylcholine biosensor reveals cholinergic differentiated SH-SY5Y cells release partial vesicle content during exocytosis 纳米尖端乙酰胆碱生物传感器显示胆碱能分化的SH-SY5Y细胞在胞吐过程中释放部分囊泡内容物

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2026-10-01 Epub Date: 2026-02-26 DOI: 10.1016/j.bioelechem.2026.109260

Yuanmo Wang , Ajay Pradhan , Pankaj Gupta , Jörg Hanrieder , Henrik Zetterberg , Ann-Sofie Cans

Acetylcholine (ACh) is a central neurotransmitter in cognitive function, motor control, and synaptic modulation, yet its electrochemical inactivity and the rapid kinetics of exocytosis have hindered real-time quantal measurements. Micrometer-scale enzymatic ACh biosensors previously enabled sub-millisecond extracellular recordings but were too large for synaptic positioning and intracellular recordings. Here we present a short, ultrafast and low-noise amperometric ACh biosensor based on a needle-shaped carbon fiber nanotip electrode functionalized with gold nanoparticles and enzymes. The miniaturized geometry allows precise placement at neurite release sites and minimally invasive insertion into the cell cytoplasm, enabling high-temporal resolution monitoring of presynaptic exocytosis together with quantification of intracellular ACh vesicle content. We applied this platform to differentiated human cholinergic SH-SY5Y neuroblastoma cells, an established yet underutilized cell model for cholinergic signaling. The nanotip sensor successfully captured amperometric spikes from both intracellular vesicle burst events and presynaptic ACh release. Intracellular events released a larger amount of ACh than presynaptic exocytosis events, indicating a predominance of partial exocytosis mode in these cells. These results demonstrate the nanotip ACh biosensor as a unique tool for probing fusion pore dynamics at subcellular resolution and for providing quantitative insight into the quantal nature of cholinergic signaling in human neuronal models.

乙酰胆碱（ACh）是认知功能、运动控制和突触调节的中枢神经递质，但它的电化学不活性和胞吐的快速动力学阻碍了实时量子测量。微米级的酶促ACh生物传感器以前可以进行亚毫秒级的细胞外记录，但对于突触定位和细胞内记录来说太大了。本文提出了一种短、超快、低噪声的基于针状碳纤维纳米电极的ACh生物传感器，该电极具有金纳米粒子和酶的功能。小型化的几何结构允许精确放置在神经突释放位点，并微创插入细胞质，从而实现突触前胞吐的高时间分辨率监测以及细胞内ACh囊泡含量的定量。我们将该平台应用于分化的人胆碱能SH-SY5Y神经母细胞瘤细胞，这是一种已建立但未充分利用的胆碱能信号传导细胞模型。纳米尖端传感器成功捕获了细胞内囊泡破裂事件和突触前乙酰胆碱释放的电流峰值。细胞内事件比突触前胞吐事件释放更多的乙酰胆碱，表明这些细胞以部分胞吐模式为主。这些结果表明，纳米尖端ACh生物传感器是一种独特的工具，可以在亚细胞分辨率下探测融合孔动力学，并为人类神经元模型中胆碱能信号的量子性质提供定量的见解。

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引用次数: 0

Electrochemistry of redox enzymes: from functional enzyme immobilization to enzymatic bio-electrochemical devices (tutorial) 氧化还原酶的电化学：从功能酶固定化到酶生物电化学装置（教程）

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2026-10-01 Epub Date: 2026-03-04 DOI: 10.1016/j.bioelechem.2026.109265

A. Guessab, I. Mazurenko, E. Lojou, A. de Poulpiquet

Electrochemistry of redox enzymes is a multidisciplinary field, and students who join labs specialized in bio-electrochemistry have various backgrounds: chemistry, biology, biotechnologies, nanotechnologies, physics, etc., so that their level in the involved disciplines is often heterogeneous, and they sometimes lack the basic knowledge about either of the fields concerned by their project (physical chemistry, electrochemistry, enzymology, etc.). Projects that gather experienced researchers from these different areas also sometimes suffer from a lack of understanding between the partners. This tutorial, which follows a lecture given at the first winter school of the French group of bio-electrochemistry (GFB), aims at guiding newcomers in the field and provides advice for more in-depth and specialized literature. It seeks to provide a solid theoretical and experimental foundation. Redox enzymes and the basic methods of immobilization at the electrode are introduced. The mechanisms of direct and mediated electron transfers are explained. An important electrochemical method, cyclic voltammetry, is explained, and the article describes how to extract information about enzyme/electrode and catalytic reactions. Finally, the tutorial presents two enzymatic bio-electrochemical devices, enzymatic fuel cells and enzymatic biosensors, and provides a guide for their electrochemical characterization.

氧化还原酶的电化学是一个多学科领域，加入生物电化学实验室的学生有不同的背景：化学、生物学、生物技术、纳米技术、物理学等，因此他们在所涉及的学科中的水平往往是异质的，有时他们缺乏项目所涉及的任何一个领域（物理化学、电化学、酶学等）的基础知识。从这些不同领域聚集经验丰富的研究人员的项目有时也会因合作伙伴之间缺乏理解而受到影响。本教程是继法国生物电化学小组（GFB）第一届冬季学校的讲座之后编写的，旨在指导该领域的新手，并为更深入和专业的文献提供建议。它力求提供一个坚实的理论和实验基础。介绍了氧化还原酶及其电极固定化的基本方法。解释了直接和间接电子转移的机理。介绍了一种重要的电化学方法——循环伏安法，并介绍了如何提取酶/电极和催化反应的信息。最后，本教程介绍了两种酶生物电化学装置，酶燃料电池和酶生物传感器，并提供了它们的电化学表征指南。

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引用次数: 0

Proof-of-concept bioelectrochemical characterization of Mycolicibacterium smegmatis for diagnostic applications 耻垢分枝杆菌诊断应用的概念验证生物电化学表征

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2026-10-01 Epub Date: 2026-03-09 DOI: 10.1016/j.bioelechem.2026.109277

Sunday Olakunle Oguntomi , Abhipsa Sahu , Yili Yang , Enrico Marsili

Elevated urease activity in tuberculosis-infected individuals is often suggestive of bacterial virulence by promoting survivability and establishment of the causative pathogen (Mycobacterium tuberculosis) in the host. Thus, heightened urease activity could be a promising biomarker for screening pathogenic mycobacterial strains. In this proof-of-concept study, we evaluate the suitability of electrochemical techniques for direct screening of mycobacterial strains based on urease activity. Mycolicibacterium smegmatis wildtype (WT), a derived knockout mutant of the LCP protein family (∆0107) with high urease activity, and its complement strain (c-0107) were coated in polydopamine (PDA) and immobilized on a screen-printed electrode (SPE) for microscopy and electrochemical characterization, in presence of exogenous redox mediators: potassium ferricyanide, anthraquinone, and riboflavin. Microscopy showed compatibility of PDA as a coating material for mycobacterial cells. Electrochemical analysis results indicated enhanced M. smegmatis electroactivity in the presence of anthraquinone, with higher current output in the strain with high urease activity. Experiments at different urea concentrations suggest that urea is not required for this screening approach. This work shows that electrochemical data can potentially distinguish mycobacterial strains based on urease activity. It also lays a groundwork in the model mycobacteria M. smegmatis, which could facilitate development of assays for pathogenic mycobacterial species.

结核菌感染个体的脲酶活性升高，通常通过促进宿主的生存能力和致病病原体（结核分枝杆菌）的建立，提示细菌的毒力。因此，提高脲酶活性可能是筛选致病性分枝杆菌菌株的一个有希望的生物标志物。在这项概念验证研究中，我们评估了基于脲酶活性的电化学技术直接筛选分枝杆菌菌株的适用性。在外源性氧化还原介质铁氰化钾、蒽醌和核黄素的存在下，将LCP蛋白家族（∆0107）的敲除突变体——耻毛分枝杆菌野生型（WT）及其补体菌株（c-0107）包被聚多巴胺（PDA），并固定在屏幕印刷电极（SPE）上进行显微镜和电化学表征。显微镜显示PDA作为分枝杆菌细胞包衣材料的相容性。电化学分析结果表明，蒽醌的存在增强了耻垢分枝杆菌的电活性，且菌株具有较高的脲酶活性。不同尿素浓度的实验表明，这种筛选方法不需要尿素。这项工作表明电化学数据可以根据脲酶活性潜在地区分分枝杆菌菌株。这也为建立耻垢分枝杆菌模型奠定了基础，为开发致病性分枝杆菌的检测方法奠定了基础。

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引用次数: 0

Optimization of a MWCNTs-AuNPs composite-modified electrochemical immunosensor with anti-fouling property for highly sensitive and rapid detection of BNP in myocardial infarction-induced heart failure diagnosis MWCNTs-AuNPs复合修饰抗污染电化学免疫传感器在心肌梗死性心力衰竭诊断中的高灵敏度和快速检测

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2026-10-01 Epub Date: 2026-02-28 DOI: 10.1016/j.bioelechem.2026.109258

Jiaru Fu , Dewei Huang , Bolu Sun , Chengwen Li , Yuqiong Wu , Huajie Li , Jiali Kang , Haiying He , Xiangdong Wang , Yaru Zhang , Xuanxiu Da , Mian Chen , Lin Yang

Brain natriuretic peptide (BNP) is a sensitive biomarker for acute heart failure (AHF). Rapid, sensitive, and anti-fouling BNP detection in serum remains clinically unmet. Herein, a novel electrochemical immunosensor with synergistic signal amplification and anti-fouling ability was constructed via layered AuNPs/MWCNTs-AuNPs modification. Bottom AuNPs mitigate MWCNTs agglomeration and enable stable Anti-BNP immobilization via AuS bonds, MWCNTs enhance conductivity, and top AuNPs amplify signals, forming an efficient electron-transport network. A nitrocellulose (NC) membrane was laminated for anti-fouling via rapid contaminant sieving. Under optimization, the sensor shows a linear range of 7.81–8 × 10³ pg/mL for BNP, with a detection limit of 8.14 × 10⁻² pg/mL (3σ, n = 11), outperforming ELISA. It exhibits excellent selectivity (vs. cTn-I, glucose, IL-6), repeatability (RSD = 2.3%), and stability (92.4% retention over 30 days). Serum recovery rates of 102–105% confirm anti-fouling reliability. This cost-effective platform promises POCT for AHF and underpins anti-fouling biosensor development.

脑钠肽（BNP）是急性心力衰竭（AHF）的敏感生物标志物。快速、灵敏、抗污染的血清BNP检测仍未在临床上实现。本文通过层状AuNPs/MWCNTs-AuNPs修饰，构建了一种具有协同信号放大和防污能力的新型电化学免疫传感器。底部AuNPs可减轻MWCNTs团聚并通过AuS键实现稳定的Anti-BNP固定化，MWCNTs可增强电导率，顶部AuNPs可放大信号，形成高效的电子传递网络。采用快速过滤的方法制备了一种硝基纤维素（NC）防污膜。优化后，该传感器对BNP的检测线性范围为7.81 ~ 8 × 103 pg/mL，检出限为8.14 × 10-2 pg/mL (3σ, n = 11)，优于ELISA。它具有优良的选择性（相对于ctn - 1、葡萄糖、IL-6）、重复性（RSD = 2.3%）和稳定性（30天内保留率为92.4%）。102-105%的血清回收率证实了抗污染的可靠性。这个具有成本效益的平台为AHF提供了POCT，并支持了防污生物传感器的开发。

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引用次数: 0

Bioluminescence-based electrochemical sensor for dual-mode direct hydrocarbon detection in saline water utilizing Photobacterium leiognathi and d-luciferin-modified au-SPE 利用光杆菌和d-荧光素修饰的au-SPE进行盐水中碳氢化合物双模直接检测的生物发光电化学传感器

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2026-08-01 Epub Date: 2026-01-24 DOI: 10.1016/j.bioelechem.2026.109233

Arash Rasti , Muhamad Afiq Aziz , Zaira Zaman Chowdhury , Sook Mei Khor

Early detection of hydrocarbon pollution in saline ecosystems faces challenges due to their complexity and the limitations of current detection methods. Salinity, temperature variations, the presence of other organic matter, and weathering processes hinder the effectiveness of traditional techniques, while the cost and complexity of some advanced detection technologies limit their widespread application. In this study, a gold screen-printed electrode (Au-SPE) modified with d-luciferin was developed to monitor the metabolic response of Photobacterium leiognathi to hexane and aromatic hydrocarbons in saline water and seawater. The sensor works by capturing adenosine triphosphate (ATP)-dependent electron release associated with bacterial bioluminescence, which varies according to the type of hydrocarbon. D-luciferin was immobilized on the Au-SPE via a self-assembled monolayer using ethylenediamine and EDC/NHS coupling to create a biocompatible interface. CV analysis revealed time-dependent shifts of anodic and cathodic peaks from −0.5 V to +0.5 V. The presence of aromatic hydrocarbons increased both bioluminescence light emission and current, indicating metabolic stimulation. In contrast, hexane suppressed bioluminescence and decreased current, indicating metabolic inhibition. These distinct responses enable rapid and selective differentiation between different types of hydrocarbons. The developed biosensor exhibits strong potential for real-time monitoring of oil contamination and assessing water quality in saline ecosystems.

盐化生态系统中烃类污染的早期检测由于其复杂性和现有检测方法的局限性而面临挑战。盐度、温度变化、其他有机物的存在和风化过程阻碍了传统技术的有效性，而一些先进探测技术的成本和复杂性限制了它们的广泛应用。本研究利用d-荧光素修饰的金丝网印刷电极（Au-SPE）来监测光杆菌在咸水和海水中对己烷和芳香烃的代谢反应。该传感器通过捕获与细菌生物发光相关的三磷酸腺苷（ATP）依赖的电子释放来工作，这种电子释放根据碳氢化合物的类型而变化。d -荧光素通过乙二胺和EDC/NHS偶联的自组装单层固定在Au-SPE上，形成生物相容性界面。CV分析显示阳极和阴极峰从−0.5 V到+0.5 V随时间变化。芳香烃的存在增加了生物发光发光和电流，表明代谢刺激。相反，己烷抑制生物发光并降低电流，表明代谢抑制。这些不同的反应使不同类型的碳氢化合物能够快速和选择性地区分。所开发的生物传感器在实时监测石油污染和评估咸水生态系统水质方面具有很强的潜力。

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引用次数: 0

Resourceful utilization of Bougainvillea horticultural waste for synchronous degradation and power generation in MFCs 九重葛园艺废弃物在mfc同步降解和发电中的资源化利用

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2026-08-01 Epub Date: 2026-01-24 DOI: 10.1016/j.bioelechem.2026.109237

Yeling Zhou, Yicheng Wu, Bingjun Shu, Feipeng Xu

The disposal of horticultural waste derived from Bougainvillea species poses substantial environmental challenges in coastal cities of southeastern China. This study innovatively employs Bougainvillea horticultural waste (BHW) as the main substrate in microbial fuel cells (MFCs) for simultaneous organic degradation and bioenergy recovery, with an acid-pretreated BHW-fed MFC (MFC-ABG) included for comparison. Results indicated that the untreated BHW-fed MFC (MFC-BG) achieved a peak voltage of 0.401 V and sustained operation for 19 days, coupled with 78.7% polysaccharide removal. Metagenomics showed that MFC-BG significantly enriched electroactive Geobacter (29.39%) and hydrolytic Proteiniphilum (2.69%), driving lignocellulose decomposition through oxidative auxiliary enzymes (AA4/AA6). Comparatively, MFC-ABG achieved an enhanced voltage of 0.706 V and a high polysaccharide reduction efficiency of 85.6%, benefits attributable to acid-induced substrate solubilization and glycoside hydrolase (GH)-dominated enzymatic shifts. Although microbial community diversity declined in both MFC systems, MFC-BG retained a higher species richness (MFC-BG: Sobs = 28,209; MFC-ABG: Sobs = 25,746), reflecting the adaptive resilience of the associated microbial community. This study confirms BHW as a viable feedstock for MFCs and clarifies the microbial mechanisms underlying the synergistic coupling of substrate degradation and electron transfer

九重葛园艺废弃物的处理对中国东南部沿海城市的环境造成了巨大的挑战。本研究创新性地采用九重葛园艺废弃物（BHW）作为微生物燃料电池（MFC）的主要底物，同时进行有机降解和生物能源回收，并采用酸预处理的九重葛园艺废弃物投料MFC （MFC- abg）进行比较。结果表明，未经处理的bhw投喂MFC （MFC- bg）的峰值电压为0.401 V，持续运行19 d，多糖去除率为78.7%。宏基因组学显示，MFC-BG显著富集电活性地杆菌（29.39%）和水解性嗜蛋白杆菌（2.69%），通过氧化辅酶（AA4/AA6）促进木质纤维素分解。相比之下，MFC-ABG实现了0.706 V的电压增强和85.6%的多糖还原效率，这得益于酸诱导的底物增溶和糖苷水解酶（GH）主导的酶移。尽管两种MFC系统的微生物群落多样性均有所下降，但MFC- bg系统保持了较高的物种丰富度（MFC- bg: Sobs = 28,209; MFC- abg: Sobs = 25,746），反映了相关微生物群落的适应弹性。本研究证实了BHW作为MFCs的可行原料，并阐明了底物降解和电子转移协同耦合的微生物机制

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引用次数: 0

Deciphering the redox signature and reactivity of wines with differential pulse voltammetry 用差分脉冲伏安法破译葡萄酒的氧化还原特征和反应性。

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry

Pub Date : 2026-08-01 Epub Date: 2026-02-03 DOI: 10.1016/j.bioelechem.2026.109239

Alice L. Dauphin, Samuel Guilbault, Stéphane Arbault

Phenolic compounds, including polyphenols and tannins, contribute to the sensory characteristics of wines and help protect them against oxidation through their reductive properties. Linear or cyclic voltammetry methods were previously reported to monitor specific wine phenolic compounds and decipher on their antioxidant activity. Pulsed voltammetry methods improve selectivity and accuracy and recently raised further interest for wine studies. We report the use of differential pulse voltammetry-DPV to characterize each wine redox profile and reactivity. Without any prior solution preparation, DPV analysis in wine provides curves displaying several oxidation peaks assigned to families of reductive phenolic acids or anthocyanins, flavonoids and tannins. Wine redox profiles vary as a function of their color, winemaking process, grape variety, vintage, etc. DPV and cyclic voltammetry-CV allowed further to study wines when changing their composition in caffeic and gallic acids, demonstrating the reactivity between phenolic species. Finally, the oxidation of a red wine under air and oxygen-saturated conditions was monitored by colorimetric and DPV analyses, directly showing the correlation between color browning, decrease of reductive ability and dissolved oxygen level. This work demonstrates the effectiveness of DPV in directly deciphering the oxidation-reduction processes occurring during winemaking and wine ageing.

酚类化合物，包括多酚和单宁，有助于葡萄酒的感官特征，并通过其还原性帮助保护它们免受氧化。线性或循环伏安法以前报道监测特定的葡萄酒酚类化合物和破译其抗氧化活性。脉冲伏安法提高了选择性和准确性，最近引起了人们对葡萄酒研究的进一步兴趣。我们报告使用差分脉冲伏安法- dpv来表征每个葡萄酒的氧化还原剖面和反应性。在没有任何事先溶液制备的情况下，DPV分析在葡萄酒中提供了显示几个氧化峰的曲线，这些氧化峰分配给还原性酚酸或花青素，类黄酮和单宁。葡萄酒的氧化还原特性随其颜色、酿酒工艺、葡萄品种、年份等因素而变化。DPV和循环伏安法- cv可以进一步研究葡萄酒中咖啡酸和没食子酸的成分变化，证明酚类物质之间的反应性。最后，采用比色法和DPV法对某红葡萄酒在空气和氧饱和条件下的氧化过程进行了监测，直接揭示了葡萄酒颜色褐变、还原能力下降和溶解氧水平之间的相关性。这项工作证明了DPV在直接破译葡萄酒酿造和葡萄酒陈酿过程中发生的氧化还原过程中的有效性。

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引用次数: 0