Pub Date : 2025-03-12DOI: 10.1016/j.bioelechem.2025.108969
Anna Szewczyk , Nina Rembiałkowska , Marta Migocka-Patrzałek , Wojciech Szlasa , Agnieszka Chwiłkowska , Małgorzata Daczewska , Vitalij Novickij , Julita Kulbacka
This study explores the combination of jasplakinolide with electroporation (JSP + EP), a method enhancing targeted molecule delivery. CHO-K1 (Chinese hamster ovarian), C2C12 (mouse myoblast), and RD (rhabdomyosarcoma) cells were treated with jasplakinolide (50 nM) in HEPES buffer and exposed to electrical pulses (0.8–1.2 kV/cm). Cell viability was measured via the MTS assay, cytoskeleton structure was assessed with confocal microscopy, and docking studies examined jasplakinolide-actin interactions. The combination of jasplakinolide and electric pulses synergistically affected RMS cells (Rhabdomyosarcoma), causing significant cytoskeletal changes and reduced viability. Docking studies revealed that jasplakinolide interacts with both monomeric and filamentous actin, highlighting a dual mechanism. Confocal imaging showed substantial actin cytoskeleton disruption in cancer cells, with minimal effects on normal cells. Jasplakinolide combined with electric pulses can specifically target cancer cells with less cytotoxicity to normal cells, potentially reducing side effects following the clinical procedure.
{"title":"Optimizing Jasplakinolide delivery in rhabdomyosarcoma cells using pulsed electric fields (PEFs) for enhanced therapeutic impact","authors":"Anna Szewczyk , Nina Rembiałkowska , Marta Migocka-Patrzałek , Wojciech Szlasa , Agnieszka Chwiłkowska , Małgorzata Daczewska , Vitalij Novickij , Julita Kulbacka","doi":"10.1016/j.bioelechem.2025.108969","DOIUrl":"10.1016/j.bioelechem.2025.108969","url":null,"abstract":"<div><div>This study explores the combination of jasplakinolide with electroporation (JSP + EP), a method enhancing targeted molecule delivery. CHO-K1 (Chinese hamster ovarian), C2C12 (mouse myoblast), and RD (rhabdomyosarcoma) cells were treated with jasplakinolide (50 nM) in HEPES buffer and exposed to electrical pulses (0.8–1.2 kV/cm). Cell viability was measured via the MTS assay, cytoskeleton structure was assessed with confocal microscopy, and docking studies examined jasplakinolide-actin interactions. The combination of jasplakinolide and electric pulses synergistically affected RMS cells (Rhabdomyosarcoma), causing significant cytoskeletal changes and reduced viability. Docking studies revealed that jasplakinolide interacts with both monomeric and filamentous actin, highlighting a dual mechanism. Confocal imaging showed substantial actin cytoskeleton disruption in cancer cells, with minimal effects on normal cells. Jasplakinolide combined with electric pulses can specifically target cancer cells with less cytotoxicity to normal cells, potentially reducing side effects following the clinical procedure.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"165 ","pages":"Article 108969"},"PeriodicalIF":4.8,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143629266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study investigates the effect of riboflavin on microbiologically influenced corrosion (MIC) of 304 stainless steel induced by Rhodopseudomonas palustris TIE-1. Riboflavin accelerated the MIC process, deepening and expanding corrosion pits. Electrochemical results showed a significant increase in corrosion rate, especially with the addition of 40 ppm riboflavin. X-ray photoelectron spectroscopy (XPS) analysis demonstrated that the passive film underwent a compositional transformation from Fe2O3/Fe3O4/Cr2O3 to FeOOH/Cr(OH)3, accompanied by oxidative conversion of Cr2O3 to CrO3 mediated by riboflavin-facilitated extracellular electron uptake (EEU). High performance liquid chromatography (HPLC) results confirmed riboflavin degradation into lumichrome, which accelerated extracellular electron transfer (EET). Scanning electrochemical microscopy (SECM) analysis demonstrated that lumichrome's redox cycling enhanced EEU efficacy, resulting in the degradation of passive film.
{"title":"Acceleration of microbiologically influenced corrosion of 304 stainless steel caused by photolysis of riboflavin","authors":"Hao Zhang , Zhongyu Wu , Yuntian Lou , Weiwei Chang , Jingzhi Yang , Hongchang Qian , Dawei Zhang","doi":"10.1016/j.bioelechem.2025.108968","DOIUrl":"10.1016/j.bioelechem.2025.108968","url":null,"abstract":"<div><div>This study investigates the effect of riboflavin on microbiologically influenced corrosion (MIC) of 304 stainless steel induced by <em>Rhodopseudomonas palustris</em> TIE-1. Riboflavin accelerated the MIC process, deepening and expanding corrosion pits. Electrochemical results showed a significant increase in corrosion rate, especially with the addition of 40 ppm riboflavin. X-ray photoelectron spectroscopy (XPS) analysis demonstrated that the passive film underwent a compositional transformation from Fe<sub>2</sub>O<sub>3</sub>/Fe<sub>3</sub>O<sub>4</sub>/Cr<sub>2</sub>O<sub>3</sub> to FeOOH/Cr(OH)<sub>3</sub>, accompanied by oxidative conversion of Cr<sub>2</sub>O<sub>3</sub> to CrO<sub>3</sub> mediated by riboflavin-facilitated extracellular electron uptake (EEU). High performance liquid chromatography (HPLC) results confirmed riboflavin degradation into lumichrome, which accelerated extracellular electron transfer (EET). Scanning electrochemical microscopy (SECM) analysis demonstrated that lumichrome's redox cycling enhanced EEU efficacy, resulting in the degradation of passive film.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"165 ","pages":"Article 108968"},"PeriodicalIF":4.8,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143643021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-06DOI: 10.1016/j.bioelechem.2025.108951
Yushuang Liu , Mingxuan Wang , Guiqi Zhou , Ying Zhang , Wenfeng Hai
The development of highly sensitive methods for detecting infectious diseases is crucial for preventing disease spread. In this study, a novel sensing platform for detecting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pathogens was developed by combining a magnetic metal-organic framework (Fe3O4@MIL-100) with graphene field-effect transistors (GFET). The Fe3O4@MIL-100 magnetic MOF was functionalized with SARS-CoV-2-specific antibodies, enabling highly selective pathogen capture in a phosphate-buffered solution. Following magnetic separation, the captured pathogens were detected using GFETs, with a linear detection range of 1 ag/mL to 10 ng/mL and a detection limit as low as 8.60 ag/mL. Furthermore, the platform has been successfully applied to human serum samples, highlighting its remarkable potential for practical application.
{"title":"Magnetic MOF-based sensing platform integrated with graphene field-effect transistors for ultrasensitive detection of infectious disease","authors":"Yushuang Liu , Mingxuan Wang , Guiqi Zhou , Ying Zhang , Wenfeng Hai","doi":"10.1016/j.bioelechem.2025.108951","DOIUrl":"10.1016/j.bioelechem.2025.108951","url":null,"abstract":"<div><div>The development of highly sensitive methods for detecting infectious diseases is crucial for preventing disease spread. In this study, a novel sensing platform for detecting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pathogens was developed by combining a magnetic metal-organic framework (Fe<sub>3</sub>O<sub>4</sub>@MIL-100) with graphene field-effect transistors (GFET). The Fe<sub>3</sub>O<sub>4</sub>@MIL-100 magnetic MOF was functionalized with SARS-CoV-2-specific antibodies, enabling highly selective pathogen capture in a phosphate-buffered solution. Following magnetic separation, the captured pathogens were detected using GFETs, with a linear detection range of 1 ag/mL to 10 ng/mL and a detection limit as low as 8.60 ag/mL. Furthermore, the platform has been successfully applied to human serum samples, highlighting its remarkable potential for practical application.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"165 ","pages":"Article 108951"},"PeriodicalIF":4.8,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143579710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-03DOI: 10.1016/j.bioelechem.2025.108965
Wenjing Xu, Yanan Zhao, Feng Gao, Xuan Zheng, Fengping Zhan, Qingxiang Wang
The integration of aptamer chemistry with innovative functional materials such as nanozymes offers new opportunities for the development of the superior electrochemical biosensors. Herein, we introduce a rod-like nanocomposite of Ce-MOF-808@CeO2 bearing intense nanozymatic activity that prepared through in-situ partial oxidation of Ce-MOF-808 to CeO2. Then, the aptamer for tetracycline (TC-Apt) with 5’-PO43− end was anchored on Ce-MOF-808@CeO2 modified screen-printed electrode, thereby assembling a label-free electrochemical aptasensor. Electrochemical and spectroscopic assays reveal that the derived CeO2 can effectively promote the nanozyme activity of Ce-MOF-808 as a cocatalyst. Electrochemical biosensing shows that, the capture of tetracycline (TC) to the electrode surface by the aptamer chemistry significantly inhibits the catalytic activity of Ce-MOF-808@CeO2. Thus, TC can be analyzed by monitoring the catalytic signal of the biosensor to H2O2. Leveraging the exceptional catalytic activity of Ce-MOF-808@CeO2, coupled with the high specificity of the aptamer, TC can be analyzed in a wide kinetic range from 1 pM to 100 nM, with a low detection limit of 0.21 pM. The aptasensor is also applicable for the accurate detection of TC residues in fresh shrimp samples, showcasing its potential for practical applications in the monitoring of food safety.
{"title":"In-situ integrated Ce-MOF-808@CeO2 as bifunctional matrix for sensitive electrochemical-aptasensing of tetracycline in shrimp","authors":"Wenjing Xu, Yanan Zhao, Feng Gao, Xuan Zheng, Fengping Zhan, Qingxiang Wang","doi":"10.1016/j.bioelechem.2025.108965","DOIUrl":"10.1016/j.bioelechem.2025.108965","url":null,"abstract":"<div><div>The integration of aptamer chemistry with innovative functional materials such as nanozymes offers new opportunities for the development of the superior electrochemical biosensors. Herein, we introduce a rod-like nanocomposite of Ce-MOF-808@CeO<sub>2</sub> bearing intense nanozymatic activity that prepared through in-situ partial oxidation of Ce-MOF-808 to CeO<sub>2</sub>. Then, the aptamer for tetracycline (TC-Apt) with 5’-PO<sub>4</sub><sup>3−</sup> end was anchored on Ce-MOF-808@CeO<sub>2</sub> modified screen-printed electrode, thereby assembling a label-free electrochemical aptasensor. Electrochemical and spectroscopic assays reveal that the derived CeO<sub>2</sub> can effectively promote the nanozyme activity of Ce-MOF-808 as a cocatalyst. Electrochemical biosensing shows that, the capture of tetracycline (TC) to the electrode surface by the aptamer chemistry significantly inhibits the catalytic activity of Ce-MOF-808@CeO<sub>2</sub>. Thus, TC can be analyzed by monitoring the catalytic signal of the biosensor to H<sub>2</sub>O<sub>2</sub>. Leveraging the exceptional catalytic activity of Ce-MOF-808@CeO<sub>2</sub>, coupled with the high specificity of the aptamer, TC can be analyzed in a wide kinetic range from 1 pM to 100 nM, with a low detection limit of 0.21 pM. The aptasensor is also applicable for the accurate detection of TC residues in fresh shrimp samples, showcasing its potential for practical applications in the monitoring of food safety.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"165 ","pages":"Article 108965"},"PeriodicalIF":4.8,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143563045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Herein, we report on the development of a phthalocynaine based metal organic frameworks (MOF) for the detection of human epidermal growth factor receptor 2 (HER2). Phthalocyanines (Pcs) exhibit good redox properties, hence their utilization as precursors for the synthesis of Pc based MOFs. The successful preparation of the MOF was confirmed using X-ray powder diffraction (XRD), thermogravimetric analysis (TGA), Fourier transform infrared (FT-IR) spectroscopy, X-ray photoelectron spectroscopy (XPS), and Brunner Emmet Teller (BET) analysis. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were utilized for electrochemical characterization of the Co octacarboxy Pc (CoOCPc), and Co-CoOCPc-MOF modified glassy carbon electrode (GCE). Differential pulse votammetry was employed for detection of HER2, which is a biomarker for cancer. The selectivity towards HER2 biomarker was accomplished by attaching an aptamer (Apt) onto the MOF modified glassy carbon surface. The GCE/Co-CoOCPc-MOF/Nf/Apt (Nf = Nafion) showed excellent analytical parameters with lowest limit of detection of 5.4 × 10 ng/mL, good repeatability and stability.
{"title":"Phthalocyanine based metal organic frameworks for electrochemical detection of human epidermal growth factor receptor 2","authors":"Lunathi Ncwane , Philani Mashazi , Tebello Nyokong","doi":"10.1016/j.bioelechem.2025.108966","DOIUrl":"10.1016/j.bioelechem.2025.108966","url":null,"abstract":"<div><div>Herein, we report on the development of a phthalocynaine based metal organic frameworks (MOF) for the detection of human epidermal growth factor receptor 2 (HER2). Phthalocyanines (Pcs) exhibit good redox properties, hence their utilization as precursors for the synthesis of Pc based MOFs. The successful preparation of the MOF was confirmed using X-ray powder diffraction (XRD), thermogravimetric analysis (TGA), Fourier transform infrared (FT-IR) spectroscopy, X-ray photoelectron spectroscopy (XPS), and Brunner Emmet Teller (BET) analysis. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were utilized for electrochemical characterization of the Co octacarboxy Pc (CoOCPc), and Co-CoOCPc-MOF modified glassy carbon electrode (GCE). Differential pulse votammetry was employed for detection of HER2, which is a biomarker for cancer. The selectivity towards HER2 biomarker was accomplished by attaching an aptamer (Apt) onto the MOF modified glassy carbon surface. The GCE/Co-CoOCPc-MOF/Nf/Apt (Nf = Nafion) showed excellent analytical parameters with lowest limit of detection of 5.4 × 10 ng/mL, good repeatability and stability.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"165 ","pages":"Article 108966"},"PeriodicalIF":4.8,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143562867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-01DOI: 10.1016/j.bioelechem.2025.108963
María Llorente , Abraham Esteve-Núñez , Raúl Berenguer
Microbial Electrochemical Fluidized Reactors (ME-FBR) changed the paradigm for growing electroactive bacteria from a biofilm strategy to a planktonic mode, while still performing direct extracellular electron transfer from oxidative metabolism in absence of redox mediators. Glassy carbon was the material selected for growing planktonic Geobacter sulfurreducens in ME-FBR. However, the material was unable to retain cells so applications implying continuous operation have been compromised. In this context, a tailor-made chemical strategy was followed considering the large amount of cytochromes C present on the outermost membrane of bacteria form of the Geobacter genus. In this work, a commercial glassy carbon (GC) was chemically modified with surface oxygen groups (SOGs) mainly carboxylic type with high affinity for heme group of cytochrome C. The functionalized material did conserve the structural and textural features and i) promoted the biofilm formation of Geobacter using acetate as sole carbon and electron donor source, and ii) increased the current density and acetate removal rate in comparison with pristine carbon. Thus, the new material enriched in carboxylic-type SOGs facilitates a-la-carte anchorage of electroactive bacteria to move on from a planktonic-based to a biofilm-based strategy, so ME-FBR operation could be expanded from batch to continuous mode, while electrical current was still possible.
{"title":"The introduction of surface oxygen groups on fluid-like electrodes enhances biofilm growth of Geobacter sulfurreducens allowing continuous operation","authors":"María Llorente , Abraham Esteve-Núñez , Raúl Berenguer","doi":"10.1016/j.bioelechem.2025.108963","DOIUrl":"10.1016/j.bioelechem.2025.108963","url":null,"abstract":"<div><div>Microbial Electrochemical Fluidized Reactors (ME-FBR) changed the paradigm for growing electroactive bacteria from a biofilm strategy to a planktonic mode, while still performing direct extracellular electron transfer from oxidative metabolism in absence of redox mediators. Glassy carbon was the material selected for growing planktonic <em>Geobacter sulfurreducens</em> in ME-FBR. However, the material was unable to retain cells so applications implying continuous operation have been compromised. In this context, a tailor-made chemical strategy was followed considering the large amount of cytochromes C present on the outermost membrane of bacteria form of the <em>Geobacter</em> genus. In this work, a commercial glassy carbon (GC) was chemically modified with surface oxygen groups (SOGs) mainly carboxylic type with high affinity for heme group of cytochrome C. The functionalized material did conserve the structural and textural features and i) promoted the biofilm formation of <em>Geobacter</em> using acetate as sole carbon and electron donor source, and ii) increased the current density and acetate removal rate in comparison with pristine carbon. Thus, the new material enriched in carboxylic-type SOGs facilitates <em>a-la-carte</em> anchorage of electroactive bacteria to move on from a planktonic-based to a biofilm-based strategy, so ME-FBR operation could be expanded from batch to continuous mode, while electrical current was still possible.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"165 ","pages":"Article 108963"},"PeriodicalIF":4.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143579711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-01DOI: 10.1016/j.bioelechem.2025.108964
Muhammed Abdel-Hamied , Min Guo , Yiming Wei , Joachim Bansmann , Rasha M. El Nashar , Franz Oswald , Boris Mizaikoff , Christine Kranz
The detection of miRNAs serving as key biomarkers in cancer diagnostics is challenging due to their small size, low abundance, and high sequence similarity, which complicates their sensitive and selective detection. Here, we report a biosensor that combines molecularly imprinted polymers (MIPs) with peptide nucleic acids (PNAs) to achieve a sensitive and highly selective miRNA detection in RNA isolates of cancer cells. MIPs were synthesized by electropolymerization utilizing PNA-supported and pre-oriented miR-21 templates molecules, which serve as both a linker for improved template orientation and an assistive recognition element for miRNA binding. Electrochemical impedance spectroscopy (EIS) was used to detect miR-21 after optimization of the sensor architecture and the experimental parameters. The sensor exhibited excellent sensitivity and selectivity toward miR-21 even when compared to the single mismatched sequence, with a linear response of 0.5–5000 pM and a limit of detection (LoD) of 0.11 ± 0.04 pM without any amplification steps. The sensor was used to quantify miR-21 in artificial serum and in RNA isolates of cancer cells to discriminate between MCF-7 and Hela cells. This approach opens new avenues for the application of MIPs as synthetic antibodies in miRNA research and emphasizes the importance of the synergistic integration of PNA.
{"title":"A novel hybrid biosensor for miRNA detection based on peptide nucleic acids and molecularly imprinted polymers","authors":"Muhammed Abdel-Hamied , Min Guo , Yiming Wei , Joachim Bansmann , Rasha M. El Nashar , Franz Oswald , Boris Mizaikoff , Christine Kranz","doi":"10.1016/j.bioelechem.2025.108964","DOIUrl":"10.1016/j.bioelechem.2025.108964","url":null,"abstract":"<div><div>The detection of miRNAs serving as key biomarkers in cancer diagnostics is challenging due to their small size, low abundance, and high sequence similarity, which complicates their sensitive and selective detection. Here, we report a biosensor that combines molecularly imprinted polymers (MIPs) with peptide nucleic acids (PNAs) to achieve a sensitive and highly selective miRNA detection in RNA isolates of cancer cells. MIPs were synthesized by electropolymerization utilizing PNA-supported and pre-oriented miR-21 templates molecules, which serve as both a linker for improved template orientation and an assistive recognition element for miRNA binding. Electrochemical impedance spectroscopy (EIS) was used to detect miR-21 after optimization of the sensor architecture and the experimental parameters. The sensor exhibited excellent sensitivity and selectivity toward miR-21 even when compared to the single mismatched sequence, with a linear response of 0.5–5000 pM and a limit of detection (LoD) of 0.11 ± 0.04 pM without any amplification steps. The sensor was used to quantify miR-21 in artificial serum and in RNA isolates of cancer cells to discriminate between MCF-7 and Hela cells. This approach opens new avenues for the application of MIPs as synthetic antibodies in miRNA research and emphasizes the importance of the synergistic integration of PNA.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"165 ","pages":"Article 108964"},"PeriodicalIF":4.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143551712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-28DOI: 10.1016/j.bioelechem.2025.108953
Tomas Sabirovas , Milda Pleckaityte , Wojciech Nogala , Vaishali Shrivastav , Rima Budvytyte
This study demonstrates the straightforward application of scanning electrochemical microscopy (SECM) for characterizing pneumolysin-induced pores in tethered bilayer lipid membranes (tBLMs). Carbon-based nanoelectrodes with a tip radius of approximately 20 nm produced distinct feedback responses during approach curves to the sample. A positive feedback response was observed when approaching the self-assembled monolayer, while the few nanometers thick tBLMs exhibited characteristics of insulating layers, yielding a negative feedback response. Based on the computational calculations, the reconstitution of functional pneumolysin was further confirmed through electrochemical impedance spectroscopy, with a concentration of 5 nM pneumolysin resulting in an average pore density of 0.64 μm−2. Finally, we demonstrated the practical utility of SECM for visualizing pneumolysin pores within the tBLM system. These experiments highlight the versatility and cost-effectiveness of electrochemical techniques for investigating membrane integrity, toxin activity, and biomolecular interactions at the nanoscale.
{"title":"Imaging functional bacterial pore-forming toxins in tethered bilayer lipid membranes using scanning electrochemical microscopy","authors":"Tomas Sabirovas , Milda Pleckaityte , Wojciech Nogala , Vaishali Shrivastav , Rima Budvytyte","doi":"10.1016/j.bioelechem.2025.108953","DOIUrl":"10.1016/j.bioelechem.2025.108953","url":null,"abstract":"<div><div>This study demonstrates the straightforward application of scanning electrochemical microscopy (SECM) for characterizing pneumolysin-induced pores in tethered bilayer lipid membranes (tBLMs). Carbon-based nanoelectrodes with a tip radius of approximately 20 nm produced distinct feedback responses during approach curves to the sample. A positive feedback response was observed when approaching the self-assembled monolayer, while the few nanometers thick tBLMs exhibited characteristics of insulating layers, yielding a negative feedback response. Based on the computational calculations, the reconstitution of functional pneumolysin was further confirmed through electrochemical impedance spectroscopy, with a concentration of 5 nM pneumolysin resulting in an average pore density of 0.64 μm<sup>−2</sup>. Finally, we demonstrated the practical utility of SECM for visualizing pneumolysin pores within the tBLM system. These experiments highlight the versatility and cost-effectiveness of electrochemical techniques for investigating membrane integrity, toxin activity, and biomolecular interactions at the nanoscale.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"165 ","pages":"Article 108953"},"PeriodicalIF":4.8,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143534762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-26DOI: 10.1016/j.bioelechem.2025.108950
Dandan Hu , Yanhong Ye , Qianlin Zhu , Ruyue Cong , Jingran Sun , Kaiyue Hu , Yufang Hu
Analyzing uracil-DNA glycosylase (UDG) activity is essential for understanding DNA repair mechanisms in disease progression and treatment. This study presents a dual-mode DNA nano-stage biosensing platform integrating electrochemiluminescence (ECL) and electrochemical impedance spectroscopy (EIS) for highly sensitive and specific UDG detection. A DNA-prism-modified electrode immobilizes UDG-responsive elements, forming a stable and efficient detection interface. Upon UDG cleavage, released DNA fragments initiate rapid nano-stage assembly, significantly amplifying the signal output. ECL signals are produced by embedded [Ru(phen)3]2+ complexes, while EIS signals result from the reaction of 3,3′-diaminobenzidine (DAB) with H2O2, catalyzed by manganese tetrakis(4-N-methylpyridyl)porphyrin (MnTMPyP). The platform achieves an exceptional detection limit of 1.0 × 10−5 U/mL, effectively validating the inhibitory effects of UDG inhibitors. Furthermore, a strong correlation between UDG activity and HeLa cell number is demonstrated. Compared to a commercial UDG detection kit, the biosensor exhibits comparable sensitivity with enhanced versatility. Notably, UDG activity is significantly higher in cancerous cells than in normal cells, reflecting the increased DNA repair demand in malignancy. This capability to distinguish UDG activity among different cell types highlights its potential for cancer diagnostics, while this biosensor platform shows promise for broader applications in clinical diagnostics, cancer research, and drug discovery.
{"title":"Dual-mode DNA nano-stage biosensing platform for efficient detection of uracil-DNA glycosylase activity in cells","authors":"Dandan Hu , Yanhong Ye , Qianlin Zhu , Ruyue Cong , Jingran Sun , Kaiyue Hu , Yufang Hu","doi":"10.1016/j.bioelechem.2025.108950","DOIUrl":"10.1016/j.bioelechem.2025.108950","url":null,"abstract":"<div><div>Analyzing uracil-DNA glycosylase (UDG) activity is essential for understanding DNA repair mechanisms in disease progression and treatment. This study presents a dual-mode DNA nano-stage biosensing platform integrating electrochemiluminescence (ECL) and electrochemical impedance spectroscopy (EIS) for highly sensitive and specific UDG detection. A DNA-prism-modified electrode immobilizes UDG-responsive elements, forming a stable and efficient detection interface. Upon UDG cleavage, released DNA fragments initiate rapid nano-stage assembly, significantly amplifying the signal output. ECL signals are produced by embedded [Ru(phen)<sub>3</sub>]<sup>2+</sup> complexes, while EIS signals result from the reaction of 3,3′-diaminobenzidine (DAB) with H<sub>2</sub>O<sub>2</sub>, catalyzed by manganese tetrakis(4-<em>N</em>-methylpyridyl)porphyrin (MnTMPyP). The platform achieves an exceptional detection limit of 1.0 × 10<sup>−5</sup> U/mL, effectively validating the inhibitory effects of UDG inhibitors. Furthermore, a strong correlation between UDG activity and HeLa cell number is demonstrated. Compared to a commercial UDG detection kit, the biosensor exhibits comparable sensitivity with enhanced versatility. Notably, UDG activity is significantly higher in cancerous cells than in normal cells, reflecting the increased DNA repair demand in malignancy. This capability to distinguish UDG activity among different cell types highlights its potential for cancer diagnostics, while this biosensor platform shows promise for broader applications in clinical diagnostics, cancer research, and drug discovery.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"165 ","pages":"Article 108950"},"PeriodicalIF":4.8,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143519898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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