D-allulose 3-epimerase for low-calorie D-allulose synthesis: microbial production, characterization, and applications.

IF 8.1 2区 工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Critical Reviews in Biotechnology Pub Date : 2024-07-07 DOI:10.1080/07388551.2024.2368517
Xiaofang Xie, Caiming Li, Xiaofeng Ban, Hongshun Yang, Zhaofeng Li
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Abstract

D-allulose, an epimer of D-fructose at C-3 position, is a low-calorie rare sugar with favorable physiochemical properties and special physiological functions, which displays promising perspectives in the food and pharmaceutical industries. Currently, D-allulose is extremely sparse in nature and is predominantly biosynthesized through the isomerization of D-fructose by D-allulose 3-epimerase (DAEase). In recent years, D-allulose 3-epimerase as the key biocatalyst for D-allulose production has received increasing interest. The current review begins by providing a summary of D-allulose regarding its characteristics and applications, as well as different synthesis pathways dominated by biotransformation. Then, the research advances of D-allulose 3-epimerase are systematically reviewed, focusing on heterologous expression and biochemical characterization, crystal structure and molecular modification, and application in D-allulose production. Concerning the constraint of low yield of DAEase for industrial application, this review addresses the various attempts made to promote the production of DAEase in different expression systems. Also, various strategies have been adopted to improve its thermotolerance and catalytic activity, which is mainly based on the structure-function relationship of DAEase. The application of DAEase in D-allulose biosynthesis from D-fructose or low-cost feedstocks through single- or multi-enzymatic cascade reaction has been discussed. Finally, the prospects for related research of D-allulose 3-epimerase are also proposed, facilitating the industrialization of DAEase and more efficient and economical bioproduction of D-allulose.

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用于低热量 D-纤维素合成的 D-allulose 3-epimerase:微生物生产、表征和应用。
D- 阿洛酮糖是 D-果糖在 C-3 位上的表聚体,是一种低热量的稀有糖类,具有良好的理化性质和特殊的生理功能,在食品和制药行业具有广阔的前景。目前,D-阿洛糖在自然界极为稀少,主要是通过 D-阿洛糖 3-表聚酶(DAEase)对 D-果糖进行异构化而生物合成的。近年来,D-阿洛糖 3-epimerase 作为生产 D-阿洛糖的关键生物催化剂受到越来越多的关注。本综述首先概述了 D-阿洛酮糖的特点和应用,以及以生物转化为主的不同合成途径。然后,系统地综述了 D-阿洛糖 3-酰亚胺酶的研究进展,重点关注异源表达和生化表征、晶体结构和分子修饰以及在 D-阿洛糖生产中的应用。鉴于工业应用中 DAEase 产量低的限制,本综述探讨了在不同表达系统中促进 DAEase 生产的各种尝试。此外,还采用了各种策略来提高 DAEase 的耐热性和催化活性,这主要是基于 DAEase 的结构-功能关系。讨论了 DAEase 在以 D-果糖或低成本原料为原料,通过单酶或多酶级联反应进行 D-阿洛糖生物合成中的应用。最后,还提出了DAEase的相关研究前景,以促进DAEase的产业化和更高效、更经济地生物生产D-阿洛糖。
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来源期刊
Critical Reviews in Biotechnology
Critical Reviews in Biotechnology 工程技术-生物工程与应用微生物
CiteScore
20.80
自引率
1.10%
发文量
71
审稿时长
4.8 months
期刊介绍: Biotechnological techniques, from fermentation to genetic manipulation, have become increasingly relevant to the food and beverage, fuel production, chemical and pharmaceutical, and waste management industries. Consequently, academic as well as industrial institutions need to keep abreast of the concepts, data, and methodologies evolved by continuing research. This journal provides a forum of critical evaluation of recent and current publications and, periodically, for state-of-the-art reports from various geographic areas around the world. Contributing authors are recognized experts in their fields, and each article is reviewed by an objective expert to ensure accuracy and objectivity of the presentation.
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