A new geminialphasatellite associated with wheat dwarf virus identified in winter barley in France

IF 2.5 4区医学 Q3 VIROLOGY Archives of Virology Pub Date : 2024-07-10 DOI:10.1007/s00705-024-06090-8

Aijun Huang, Laurence Svanella-Dumas, C. Vitry, Armelle Marais, Chantal Faure, Thierry Candresse

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Abstract

Using a high-throughput sequencing (HTS) approach, we report the discovery of a new alphasatellite identified in a winter barley plant collected in France in 2022 that was also infected by wheat dwarf virus (WDV). The presence of the satellite and of WDV was confirmed by several independent PCR assays, and the complete genome sequence was determined. The circular satellite genome is 1424 nt long and shows typical hallmarks of members of the subfamily Geminialphasatellitinae, including a replication-associated hairpin with a CAGTATTAC sequence and a Rep-encoding open reading frame (ORF). It also possesses a second ORF, embedded in a different frame within the Rep ORF, which is also observed in clecrusatellites and a few other members of the family Alphasatellitidae. Pairwise sequence comparisons and phylogenetic analysis showed that this satellite represents a novel species. Its closest relatives are in the genus Colecusatellite, but it likely represents a new genus given its divergence from other genera of the subfamily Geminialphasatellitinae. Given that WDV was the only virus observed in coinfection with the satellite, the name "wheat dwarf virus-associated alphasatellite" is proposed for this novel agent.

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在法国冬大麦中发现与小麦矮缩病毒有关的一种新的geminialphasatellite。

利用高通量测序（HTS）方法，我们报告了 2022 年在法国采集的冬大麦植株中发现的一颗新的优势卫星，该植株也感染了小麦矮缩病毒（WDV）。卫星和 WDV 的存在通过几种独立的 PCR 检测得到了证实，并确定了完整的基因组序列。环状卫星基因组长 1424 nt，显示出 Geminialphasatellitinae 亚科成员的典型特征，包括一个带有 CAGTATTAC 序列的复制相关发夹和一个 Rep 编码开放阅读框（ORF）。它还具有第二个 ORF，嵌入 Rep ORF 的不同框架中，这在裂头蝇和 Alphasatellitidae 家族的其他一些成员中也能观察到。成对序列比较和系统进化分析表明，该卫星代表了一个新物种。它的近亲是蜗牛卫星属（Colecusatellite），但鉴于它与蜗牛卫星亚科（Geminialphasatellitinae）其他属的分化，它很可能代表一个新属。鉴于 WDV 是在与卫星共同感染中观察到的唯一病毒，因此建议将这种新型病原体命名为 "小麦矮小病毒相关字母卫星"。

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来源期刊

Archives of Virology 医学-病毒学

CiteScore

5.10

自引率

7.40%

发文量

324

审稿时长

4.5 months

期刊介绍： Archives of Virology publishes original contributions from all branches of research on viruses, virus-like agents, and virus infections of humans, animals, plants, insects, and bacteria. Coverage spans a broad spectrum of topics, from descriptions of newly discovered viruses, to studies of virus structure, composition, and genetics, to studies of virus interactions with host cells, organisms and populations. Studies employ molecular biologic, molecular genetics, and current immunologic and epidemiologic approaches. Contents include studies on the molecular pathogenesis, pathophysiology, and genetics of virus infections in individual hosts, and studies on the molecular epidemiology of virus infections in populations. Also included are studies involving applied research such as diagnostic technology development, monoclonal antibody panel development, vaccine development, and antiviral drug development.Archives of Virology wishes to publish obituaries of recently deceased well-known virologists and leading figures in virology.