Pub Date : 2026-03-19DOI: 10.1007/s00705-026-06571-y
Sumesh M Kakkunnath, Sophie Bouvaine, Siji P Kavil, M N Maruthi
Cassava brown streak disease (CBSD) poses a major threat to cassava production in Africa. Identifying cassava proteins that interact with cassava brown streak virus (CBSV), the major causal virus, can help elucidate the mechanisms of infection and resistance. Here we constructed a cassava cDNA library and screened for proteins interacting with CBSV viral genome-linked protein (VPg) using yeast two-hybrid assays, identifying 36 interactors. Four candidates were validated in Nicotiana benthamiana via bimolecular fluorescence complementation. Functional categories included chloroplast proteins, ribosomal components, chaperones, metabolic enzymes, and defence-related proteins. In prior RNA-seq datasets from CBSV or UCBSV-inoculated cassava, comprising the susceptible variety Albert and the resistant variety Namikonga, 16 VPg-interacting genes were identified among the differentially expressed genes (|log₂FC| > 1), with 2 detected in Albert and 15 in Namikonga. These results indicate that VPg-interacting host proteins are involved in CBSV infection dynamics and also explaining the differential responses of resistant and susceptible cassava varieties, thus offering potential new molecular targets for CBSD management.
{"title":"Deciphering cassava brown streak virus infection in cassava through VPg mediated host protein interactions.","authors":"Sumesh M Kakkunnath, Sophie Bouvaine, Siji P Kavil, M N Maruthi","doi":"10.1007/s00705-026-06571-y","DOIUrl":"10.1007/s00705-026-06571-y","url":null,"abstract":"<p><p>Cassava brown streak disease (CBSD) poses a major threat to cassava production in Africa. Identifying cassava proteins that interact with cassava brown streak virus (CBSV), the major causal virus, can help elucidate the mechanisms of infection and resistance. Here we constructed a cassava cDNA library and screened for proteins interacting with CBSV viral genome-linked protein (VPg) using yeast two-hybrid assays, identifying 36 interactors. Four candidates were validated in Nicotiana benthamiana via bimolecular fluorescence complementation. Functional categories included chloroplast proteins, ribosomal components, chaperones, metabolic enzymes, and defence-related proteins. In prior RNA-seq datasets from CBSV or UCBSV-inoculated cassava, comprising the susceptible variety Albert and the resistant variety Namikonga, 16 VPg-interacting genes were identified among the differentially expressed genes (|log₂FC| > 1), with 2 detected in Albert and 15 in Namikonga. These results indicate that VPg-interacting host proteins are involved in CBSV infection dynamics and also explaining the differential responses of resistant and susceptible cassava varieties, thus offering potential new molecular targets for CBSD management.</p>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"171 4","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12999836/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147479812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-19DOI: 10.1007/s00705-026-06566-9
Xiaoli Mo, Bohan Yu, Xudong Tang
{"title":"Viral subversion of neddylation: dual roles in replication efficiency and evasion of antiviral immunity.","authors":"Xiaoli Mo, Bohan Yu, Xudong Tang","doi":"10.1007/s00705-026-06566-9","DOIUrl":"https://doi.org/10.1007/s00705-026-06566-9","url":null,"abstract":"","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"171 4","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147484309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-18DOI: 10.1007/s00705-026-06568-7
Haroon Afzal, Muhammad Umar, Nguyen-Thanh Hoa, Guan-Ming Ke, Li-Ting Cheng
{"title":"Enhancing the immunogenicity of a recombinant PEDV subunit vaccine: the role of lipoprotein signal peptide.","authors":"Haroon Afzal, Muhammad Umar, Nguyen-Thanh Hoa, Guan-Ming Ke, Li-Ting Cheng","doi":"10.1007/s00705-026-06568-7","DOIUrl":"https://doi.org/10.1007/s00705-026-06568-7","url":null,"abstract":"","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"171 4","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147479868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-18DOI: 10.1007/s00705-026-06551-2
Alexander Chepurnov, Svetlana Miroshnichenko, Mickhail Ivanov, Mariya Solomatina, Elena Kazachinskaia, Evgeniya Kazachkova, Yulia Kononova, Mickhail Voevoda, Arseniya Shelemba, Oksana Mishchenko, Aleksey Surovyatkin, Marina Gulyaeva, Alexander Shestopalov
{"title":"Specific features of the infection caused by SARS-CoV-2 variants in Vero cell culture.","authors":"Alexander Chepurnov, Svetlana Miroshnichenko, Mickhail Ivanov, Mariya Solomatina, Elena Kazachinskaia, Evgeniya Kazachkova, Yulia Kononova, Mickhail Voevoda, Arseniya Shelemba, Oksana Mishchenko, Aleksey Surovyatkin, Marina Gulyaeva, Alexander Shestopalov","doi":"10.1007/s00705-026-06551-2","DOIUrl":"https://doi.org/10.1007/s00705-026-06551-2","url":null,"abstract":"","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"171 4","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147479946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-18DOI: 10.1007/s00705-026-06560-1
Thanh Tien Tran, Nicholas M Andronicos, Natkunam Ketheesan, Stephen W Walkden-Brown, Priscilla F Gerber
{"title":"Immune responses in chicken mucosal lymphoid tissues following oral and eye drop vaccination and revaccination with live modified vaccine of infectious laryngotracheitis virus (ILTV).","authors":"Thanh Tien Tran, Nicholas M Andronicos, Natkunam Ketheesan, Stephen W Walkden-Brown, Priscilla F Gerber","doi":"10.1007/s00705-026-06560-1","DOIUrl":"10.1007/s00705-026-06560-1","url":null,"abstract":"","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"171 4","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12999625/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147479934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Virophages parasitize the replication of co-infecting giant viruses within eukaryotic cells, forming tripartite cell-virus-virophage (CVv) systems. Tripartite interactions are well-documented in protozoa, yet comparable systems in algae remain largely unexplored at the experimental level. Here, we report an experimentally validated CVv system involving the green, single-celled microalga Chlorella sp. DSL01, Dishui Lake large algal virus 1 (DSLLAV1), and multiple Dishui Lake virophages (DSLVs). Inoculation of Chlorella sp. DSL01 at low MOI established laboratory co-cultures in which time-series PCR detected DSLLAV1 early but not after Day 10, whereas all tested virophages persisted. Metagenomic profiling of the terminal supernatant (end-point sample) indicated a virophage-dominated assemblage with DSLV3 most represented. Droplet digital PCR at discrete time points (Days 5, 10, and 15) then provided absolute counts for DSLLAV1 and DSLV1/3/7, corroborating an early DSLLAV1 peak followed by collapse and/or a delayed rise of multiple virophages coincident with host growth recovery. Nested PCR on the algal pellet detected virophages DSLV1/3/7 but not DSLLAV1. Together, these results demonstrate that Chlorella sp. DSL01 supports co-infection by DSLLAV1 and multiple virophages, establishing an experimentally validated algal CVv system and revealing multi-virophage participation in freshwater algal virus-virophage-host dynamics.
{"title":"Microalga-virus-virophage coculture reveals co-infection of multi-virophages with a giant virus.","authors":"Ting Chu, Qinran Wang, Chen Hu, Jiabei Yu, Lanming Chen, Yongxin Yu, Yongjie Wang","doi":"10.1007/s00705-026-06547-y","DOIUrl":"https://doi.org/10.1007/s00705-026-06547-y","url":null,"abstract":"<p><p>Virophages parasitize the replication of co-infecting giant viruses within eukaryotic cells, forming tripartite cell-virus-virophage (CVv) systems. Tripartite interactions are well-documented in protozoa, yet comparable systems in algae remain largely unexplored at the experimental level. Here, we report an experimentally validated CVv system involving the green, single-celled microalga Chlorella sp. DSL01, Dishui Lake large algal virus 1 (DSLLAV1), and multiple Dishui Lake virophages (DSLVs). Inoculation of Chlorella sp. DSL01 at low MOI established laboratory co-cultures in which time-series PCR detected DSLLAV1 early but not after Day 10, whereas all tested virophages persisted. Metagenomic profiling of the terminal supernatant (end-point sample) indicated a virophage-dominated assemblage with DSLV3 most represented. Droplet digital PCR at discrete time points (Days 5, 10, and 15) then provided absolute counts for DSLLAV1 and DSLV1/3/7, corroborating an early DSLLAV1 peak followed by collapse and/or a delayed rise of multiple virophages coincident with host growth recovery. Nested PCR on the algal pellet detected virophages DSLV1/3/7 but not DSLLAV1. Together, these results demonstrate that Chlorella sp. DSL01 supports co-infection by DSLLAV1 and multiple virophages, establishing an experimentally validated algal CVv system and revealing multi-virophage participation in freshwater algal virus-virophage-host dynamics.</p>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"171 4","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147472561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-17DOI: 10.1007/s00705-026-06600-w
Anh The Nguyen, Hong Thi Thu Ta, Anh Thi Hai Dao, Hung Manh Vu, Nghia Duy Ngu, Duong Nhu Tran, Hiroyuki Shimizu, Yorihiro Nishimura, Thi Nguyen Hoa-Tran
{"title":"Correction: Lineage replacement of coxsackievirus A10 in Vietnam associated with increased detection and altered disease severity grading of hand, foot, and mouth disease.","authors":"Anh The Nguyen, Hong Thi Thu Ta, Anh Thi Hai Dao, Hung Manh Vu, Nghia Duy Ngu, Duong Nhu Tran, Hiroyuki Shimizu, Yorihiro Nishimura, Thi Nguyen Hoa-Tran","doi":"10.1007/s00705-026-06600-w","DOIUrl":"https://doi.org/10.1007/s00705-026-06600-w","url":null,"abstract":"","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"171 4","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147472635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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