The role of reactive oxygen species in regulation of the plasma membrane H+-ATPase activity in Masson pine (Pinus massoniana Lamb.) roots responding to acid stress.

IF 3.5 2区 农林科学 Q1 FORESTRY Tree physiology Pub Date : 2024-08-03 DOI:10.1093/treephys/tpae083
Sijie Zhou, Wenxin Wang, Ping Wang, Huiyan Ma, Wenhui Li
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Abstract

To understand the role of reactive oxygen species (ROS) in regulation of the plasma membrane (PM) H+-ATPase in acid-stressed Masson pine roots, different acidity (pH 6.6 as the control, pH 5.6 and pH 4.6) of simulated acid rain (SAR) added with and without external chemicals (H2O2, enzyme inhibitors and ROS scavenger) was prepared. After 30 days of SAR exposure, the plant morphological phenotype attributes, levels of cellular ROS and lipid peroxidation, enzymatic activities of antioxidants, PM nicotinamide adenine dinucleotide phosphate (NADPH) oxidase activity and PM H+-ATPase activity in pine seedlings were measured. Compared with the control, the growth of pine seedlings exposed to SAR in the presence or absence of H2O2 was well-maintained, but the application of Na3VO4, 1,3-dimethyl-2-thiourea, N, N-dimethylthiourea (DMTU) and diphenyleneiodonium chloride (DPI) caused a substantial growth inhibition. In addition, SAR exposure, SAR with H2O2 treatment, and SAR with Na3VO4 treatment increased the cellular H2O2 content, O2- content and malondialdehyde (MDA) content, while the use of DMTU and DPI lead to relatively low levels. Similarly, the enzymatic activities of antioxidants, PM NADPH oxidase and PM H+-ATPase in acid stressed pine seedlings elevated with the increasing acidity. A significant stimulation of these enzymatic activities obtained from SAR with H2O2 treatment was observed, whereas which decreased obviously with the addition of Na3VO4, DMTU and DPI (P < 0.05). Moreover, a positive correlation was found between plant morphological attributes and the PM H+-ATPase activity (P < 0.05). Besides, the PM H+-ATPase activity positively correlated with the cellular ROS contents and the enzymatic activities of antioxidants and PM NADPH oxidase (P < 0.05). Therefore, the PM H+-ATPase is instrumental in the growth of pine seedlings resisting to acid stress by enhancing its activity. The process involves the signaling transduction of cellular ROS and coordination with PM NADPH oxidase.

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活性氧在调节马松(Pinus massoniana Lamb.)根应对酸胁迫的质膜 H +-ATPase 活性中的作用。
为了解活性氧(ROS)在酸胁迫马尾松根质膜(PM)H+-ATP酶调控中的作用,制备了添加或不添加外部化学物质[H2O2、酶抑制剂和ROS清除剂]的不同酸度(pH 6.6为对照、pH 5.6和pH 4.6)的模拟酸雨(SAR)。模拟酸雨暴露 30 天后,测定了松树幼苗的形态表型属性、细胞 ROS 和脂质过氧化水平、抗氧化剂的酶活性、PM 烟酸腺嘌呤二核苷酸磷酸(NADPH)氧化酶活性和 PM H+-ATP 酶活性。与对照组相比,在有或没有 H2O2 的情况下,暴露于 SAR 的松树幼苗都能很好地保持生长,但施加 Na3VO4、1,3-二甲基-2-硫脲、N,N-二甲基硫脲(DMTU)和二苯基氯化碘(DPI)会导致生长受到严重抑制。此外,SAR 暴露、SAR 与 H2O2 处理以及 SAR 与 Na3VO4 处理都会增加细胞中的 H2O2 含量、O2-含量和丙二醛(MDA)含量,而使用 DMTU 和 DPI 则相对较低。同样,酸胁迫松树幼苗中抗氧化剂、PM NADPH氧化酶和PM H+-ATP酶的酶活性也随着酸度的增加而升高。观察到 H2O2 处理 SAR 会明显刺激这些酶的活性,而加入 Na3VO4、DMTU 和 DPI 会明显降低这些酶的活性(P<0.05)。
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来源期刊
Tree physiology
Tree physiology 农林科学-林学
CiteScore
7.10
自引率
7.50%
发文量
133
审稿时长
1 months
期刊介绍: Tree Physiology promotes research in a framework of hierarchically organized systems, measuring insight by the ability to link adjacent layers: thus, investigated tree physiology phenomenon should seek mechanistic explanation in finer-scale phenomena as well as seek significance in larger scale phenomena (Passioura 1979). A phenomenon not linked downscale is merely descriptive; an observation not linked upscale, might be trivial. Physiologists often refer qualitatively to processes at finer or coarser scale than the scale of their observation, and studies formally directed at three, or even two adjacent scales are rare. To emphasize the importance of relating mechanisms to coarser scale function, Tree Physiology will highlight papers doing so particularly well as feature papers.
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