Portable fiber-based double nanohole optical tweezer for trapping small proteins

IF 4.6 Q1 OPTICS Journal of Physics-Photonics Pub Date : 2024-07-09 DOI:10.1088/2515-7647/ad5bd0
Veerpal Kaur, Demelza Wright, Samuel Mathew, Matthew Peters, Maria Zacharopoulou, Shang Hua Yang, Laura S Itzhaki, Ivet Bahar, Reuven Gordon
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Abstract

We demonstrate the trapping and analysis of individual proteins using a portable optical fiber tweezer setup with a double-nanohole in a gold film coating the fiber’s end and aligned with the fiber core. The instrument was used to trap and analyze cytochrome c, carbonic anhydrase, bovine serum albumin, and PR65 (wild-type and various point mutants). This approach was compared with a free-space optical tweezer setup that requires alignment of the laser focus to the aperture, whereas the fiber-based approach is both portable and alignment-free, which holds promise for applications in antibody discovery, small molecule drug discovery, protein interaction analysis and other applications using the standard well-plate format.
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用于捕获小蛋白质的便携式光纤双纳米孔光学镊子
我们展示了使用便携式光纤镊子装置捕获和分析单个蛋白质的过程,该装置在光纤末端涂有一层金膜,金膜上的双纳米孔与光纤芯对齐。该仪器用于捕获和分析细胞色素 c、碳酸酐酶、牛血清白蛋白和 PR65(野生型和各种点突变体)。这种方法与自由空间光学镊子装置进行了比较,后者需要将激光焦点对准光圈,而基于光纤的方法既便携又无需对准,有望应用于抗体发现、小分子药物发现、蛋白质相互作用分析以及使用标准孔板格式的其他应用。
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来源期刊
CiteScore
10.70
自引率
0.00%
发文量
27
审稿时长
12 weeks
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