Epidermal cell proliferation and differentiation in the beak of tadpoles of Rana dalmatina

IF 1.1 4区 生物学 Q4 ANATOMY & MORPHOLOGY Acta Zoologica Pub Date : 2024-07-08 DOI:10.1111/azo.12512
Lorenzo Alibardi
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Abstract

Epidermal cell proliferation and differentiation during formation of the beak in tadpoles of Rana dalmatina. Acta Zoologica (Stockolm). The beak utilized in tadpoles of R. dalmatina for crushing vegetables is formed of cornified cell layers. A row of large wedge‐like cells forms a central pile that produces the sharp corneous lamina of the beak. The labial and oral surfaces of the beaks also accumulate corneous material during proliferation and differentiation of keratinocytes from the epidermis to form a sheath around the central corneous cells. Corneocytes are shed during the growth of tadpoles. Electron microscopy reveals that keratin and mucus granules are main components of these keratinocytes. Electron‐dense organelles of unknown composition and with inside lamellae are also present. Using immunohistochemistry after post‐injection of 5BrdU, a marker of cell proliferation, the main timing of formation of the beak has been determined. After 2–5 h from injection, labelled nuclei of keratinocytes are seen in the basal layer, and sparse suprabasal labelled nuclei are detected after 5 h. Labelled keratinocytes are seen in suprabasal layers at 2 days post‐injection. At 5 and 6 days labelled keratinocytes are present in central corneous cells and in the lateral shell of corneocytes forming the labial and oral beak sides. Shedding a complete stratum corneum likely takes more days, but this depends on feeding usage.
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达尔马提那蛙喙表皮细胞的增殖和分化
达玛提娜蝌蚪喙形成过程中表皮细胞的增殖和分化。动物学报》(斯德哥尔摩)。达尔马提那蝌蚪用来压碎蔬菜的喙是由粟状细胞层形成的。一排大的楔形细胞形成一个中心堆,形成喙的锋利角质层。在表皮的角质细胞增殖和分化过程中,喙的唇面和口面也会堆积角质物质,在中央角质细胞周围形成一层鞘。角质细胞在蝌蚪的生长过程中脱落。电子显微镜显示,角蛋白和粘液颗粒是这些角质细胞的主要成分。此外,还存在成分不明的电子致密细胞器,其内部有薄片。在注射细胞增殖标记物 5BrdU 后,使用免疫组织化学方法确定了喙的主要形成时间。注射 2-5 h 后,基底层出现标记的角质细胞核,5 h 后检测到稀疏的基底上层标记核。注射后 5 天和 6 天,标记的角质细胞出现在中央角质细胞以及形成唇侧和口喙侧的角质细胞侧壳中。脱落完整的角质层可能需要更多天,但这取决于喂食情况。
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来源期刊
Acta Zoologica
Acta Zoologica 生物-动物学
CiteScore
2.90
自引率
8.30%
发文量
35
审稿时长
>12 weeks
期刊介绍: Published regularly since 1920, Acta Zoologica has retained its position as one of the world''s leading journals in the field of animal organization, development, structure and function. Each issue publishes original research of interest to zoologists and physiologists worldwide, in the field of animal structure (from the cellular to the organismic level) and development with emphasis on functional, comparative and phylogenetic aspects. Occasional review articles are also published, as well as book reviews.
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