Biochemical Characterization and Electrochemical Cultivation of Halotolerant Autotrophic Iron-Oxidizing Bacteria Isolated from Electroplating Wastewater Sludge in Japan

IF 1 4区生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Applied Biochemistry and Microbiology Pub Date : 2024-07-10 DOI:10.1134/S0003683824604025

A. Hosoda, T. Takagi, A. Shimizu, A. Kato, H. Masui, T. Kato

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Abstract

The aerobic and rod-shaped FO1 strain of iron-oxidizing bacteria was isolated from electroplating wastewater sludge using colloidal silica plates. Bacterial growth occurred at 25–30°C (optimum temperature, 27°C), pH 2.0–3.5, and in the presence of 1.0–4.0% NaCl. The X-ray diffraction analysis identified the precipitates that were formed during cell growth as jarosite. A biochemical analysis, including the assessments of the whole-cell fatty acids, quinone, and DNA G+C contents, revealed that FO1 was closely related to the genus Acidihalobacter. The 16S rRNA sequence of the FO1 gene was similar to those of the Acidihalobacter prosperus DSM 5130 (97% similarity), Ah. aeolianus DSM 14174 (100%), and Ah. yilgarnensis DSM 105917 (100%) strains. Antibiotic sensitivity tests of strain FO1 revealed that it was tolerant for gentamicin, kanamycin, and tetracycline, whereas it was sensitive to ampicillin and chloramphenicol. Moreover, strain FO1 was able to grow in a microbial electrolysis cell with continuous reduction of Fe³⁺ to Fe²⁺ on the cathode, albeit with a slight inhibition of cell growth. This study provides information regarding the genetics, bioleaching of metals, and electrochemical cultivation of halotolerant iron-oxidizing bacteria in the genus Acidihalobacter.

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从日本电镀废水污泥中分离出的耐卤自养型氧化铁细菌的生化特征和电化学培养

摘要利用胶体二氧化硅板从电镀废水污泥中分离出好氧、杆状的铁氧化细菌 FO1 菌株。细菌的生长温度为 25-30℃（最适温度为 27℃），pH 值为 2.0-3.5，NaCl 含量为 1.0-4.0%。通过 X 射线衍射分析，确定细胞生长过程中形成的沉淀物为焦石棉。生化分析（包括全细胞脂肪酸、醌和 DNA G+C 含量的评估）显示，FO1 与酸杆菌属关系密切。FO1 基因的 16S rRNA 序列与 Acidihalobacter prosperus DSM 5130（相似度为 97%）、Ah. aeolianus DSM 14174（100%）和 Ah. yilgarnensis DSM 105917（100%）菌株相似。菌株 FO1 的抗生素敏感性测试显示，它对庆大霉素、卡那霉素和四环素耐受，而对氨苄西林和氯霉素敏感。此外，菌株 FO1 还能在微生物电解池中生长，阴极上的 Fe3+ 不断还原为 Fe2+，但细胞生长受到轻微抑制。这项研究提供了有关耐卤铁氧化细菌（Acidihalobacter）属的遗传学、金属生物浸出和电化学培养的信息。

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来源期刊

Applied Biochemistry and Microbiology 生物-生物工程与应用微生物

CiteScore

1.70

自引率

12.50%

发文量

审稿时长

6-12 weeks

期刊介绍： Applied Biochemistry and Microbiology is an international peer reviewed journal that publishes original articles on biochemistry and microbiology that have or may have practical applications. The studies include: enzymes and mechanisms of enzymatic reactions, biosynthesis of low and high molecular physiologically active compounds; the studies of their structure and properties; biogenesis and pathways of their regulation; metabolism of producers of biologically active compounds, biocatalysis in organic synthesis, applied genetics of microorganisms, applied enzymology; protein and metabolic engineering, biochemical bases of phytoimmunity, applied aspects of biochemical and immunochemical analysis; biodegradation of xenobiotics; biosensors; biomedical research (without clinical studies). Along with experimental works, the journal publishes descriptions of novel research techniques and reviews on selected topics.