Therapeutic effects of curculigoside on cyclophosphamide-induced premature ovarian failure in mice.

IF 2.9 4区 医学 Q1 OBSTETRICS & GYNECOLOGY Climacteric Pub Date : 2024-08-01 Epub Date: 2024-06-19 DOI:10.1080/13697137.2024.2354742
Yuhao Meng, Yinjuan Lyu, Jian Gong, Yue Zou, Xiaocui Jiang, Min Xiao, Jianfang Guo
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Abstract

Objective: The main purpose of this study was to elucidate the anti-apoptotic effects of curculigoside (CUR) on ovarian granulosa cells (GCs) in a mouse model of cyclophosphamide (CTX)-induced premature ovarian failure (POF).

Method: Intraperitoneal injection of CTX (100 mg/kg body weight) induced POF in mice. Thirty-six female mice were divided into six groups: blank group; POF model group; low-dose CUR group; medium-dose CUR group; high-dose CUR group; and estradiol benzoate group. Mice were orally administered for 28 consecutive days. Twenty-four hours after the completion of treatment, mice were weighed and euthanized, and blood was collected from the eyeball under anesthesia. The ovaries were surgically separated and weighed, and the ovarian index was calculated. Hematoxylin-eosin (HE) staining was used to observe follicular development and corpus luteum morphology in the ovaries. Serum levels of follicle stimulating hormone (FSH), anti-Müllerian hormone (AMH) and estradiol (E2) were measured. Superoxide dismutase (SOD) activity, glutathione peroxidase (GSH-Px) content and malondialdehyde (MDA) levels in ovarian tissue were determined. The GC apoptosis level was measured. Western blotting was used to detect protein expression levels of Beclin-1, LC3, P62, AKT, p-AKT, mTOR and p-mTOR in the ovaries.

Results: The results showed that CUR can improve body weight and ovarian index; promote follicular development and reduce follicular atresia; improve FSH, AMH and E2 levels; downregulate MDA levels and restore antioxidant enzyme activity; inhibit the autophagy level; activate the AKT/mTOR signaling pathway; and alleviate GC apoptosis.

Conclusion: CUR improves POF by activating the AKT/mTOR signaling pathway, inhibiting autophagy and alleviating GC apoptosis.

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莪术苷对环磷酰胺诱导的小鼠卵巢早衰的治疗作用
研究目的本研究的主要目的是在环磷酰胺(CTX)诱导的卵巢早衰(POF)小鼠模型中阐明莪术苷(CUR)对卵巢颗粒细胞(GCs)的抗凋亡作用:方法:腹腔注射CTX(100毫克/千克体重)诱导小鼠卵巢早衰。将 36 只雌性小鼠分为 6 组:空白组;POF 模型组;低剂量 CUR 组;中剂量 CUR 组;高剂量 CUR 组;苯甲酸雌二醇组。小鼠连续口服 28 天。治疗结束 24 小时后,称重并安乐死小鼠,在麻醉下采集眼球血液。通过手术分离卵巢并称重,计算卵巢指数。用苏木精-伊红(HE)染色法观察卵巢中卵泡的发育和黄体的形态。测定了血清中促卵泡激素(FSH)、抗苗勒氏激素(AMH)和雌二醇(E2)的水平。测定了卵巢组织中的超氧化物歧化酶(SOD)活性、谷胱甘肽过氧化物酶(GSH-Px)含量和丙二醛(MDA)水平。还测定了 GC 细胞凋亡水平。用 Western 印迹法检测卵巢中 Beclin-1、LC3、P62、AKT、p-AKT、mTOR 和 p-mTOR 的蛋白表达水平:结果表明:CUR能改善体重和卵巢指数;促进卵泡发育,减少卵泡闭锁;改善FSH、AMH和E2水平;下调MDA水平,恢复抗氧化酶活性;抑制自噬水平;激活AKT/mTOR信号通路;缓解GC凋亡:结论:CUR可通过激活AKT/mTOR信号通路、抑制自噬和缓解GC凋亡来改善POF。
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来源期刊
Climacteric
Climacteric 医学-妇产科学
CiteScore
1.70
自引率
7.10%
发文量
53
审稿时长
1 months
期刊介绍: Climacteric is the official journal of the International Menopause Society (IMS). As an international peer-reviewed journal it publishes original research and reviews of all aspects of aging in women. Climacteric was founded by the IMS in 1998 and today has become a leading journal in the publication of peer-reviewed papers on the menopause, climacteric and mid-life health. Topics covered include endocrine changes, symptoms attributed to the menopause and their treatment, hormone replacement and alternative therapies, lifestyles, and the counselling and education of peri- and postmenopausal women. Climacteric, published bimonthly, also features regular invited reviews, editorials and commentaries on recent developments. The editorial review board of Climacteric includes leading scientific and clinical experts in the field of midlife medicine and research and is headed by its Editor-in-Chief, Professor Rod Baber of Australia. He and his team of Associate Editors act independently to set a clear editorial policy, co-ordinate peer review, and ensure a rapid response to submitted papers.
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