Nosocomial transmission of tet(x3), blaNDM-1 and blaOXA-97-carrying Acinetobacter baumannii conferring resistance to eravacycline and omadacycline, the Netherlands, March to August 2021

IF 9.9 2区医学 Q1 INFECTIOUS DISEASES Eurosurveillance Pub Date : 2024-07-11 DOI:10.2807/1560-7917.es.2024.29.28.2400019

Ditmer T Talsma, Rodrigo Monteiro, Rosario del Carmen Flores-Vallejo, Maarten Heuvelmans, Thuy-Nga Le, Antoni PA Hendrickx, Sigrid Rosema, Ianthe Maat, Jan Maarten van Dijl, Erik Bathoorn

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Abstract

Carbapenem-resistant Acinetobacter baumannii (CRAb) is an important pathogen causing serious nosocomial infections. We describe an outbreak of CRAb in an intensive care unit in the Netherlands in 2021. During an outbreak of non-resistant A. baumannii, while infection control measures were in place, CRAb isolates carrying highly similar blaNDM-1- and tet(x3)-encoding plasmids were isolated from three patients over a period of several months. The chromosomal and plasmid sequences of the CRAb and non-carbapenemase-carrying A. baumannii isolates cultured from patient materials were analysed using hybrid assemblies of short-read and long-read sequences. The CRAb isolates revealed that the CRAb outbreak consisted of two different strains, carrying similar plasmids. The plasmids contained multiple antibiotic resistance genes including the tetracycline resistance gene tet(x3), and the blaNDM-1 and blaOXA-97 carbapenemase genes. We determined minimal inhibitory concentrations (MICs) for 13 antibiotics, including the newly registered tetracycline antibiotics eravacycline and omadacycline. The CRAb isolates showed high MICs for tetracycline antibiotics including eravacycline and omadacycline, except for minocycline which had a low MIC. In this study we show the value of sequencing multidrug-resistant A. baumannii for outbreak tracking and guiding outbreak mitigation measures.

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2021年3月至8月荷兰对依拉维辛和奥美拉唑产生耐药性的携带tet(x3)、blaNDM-1和blaOXA-97的鲍曼不动杆菌的院内传播情况

耐碳青霉烯类鲍曼不动杆菌（CRAb）是引起严重院内感染的重要病原体。我们描述了 2021 年荷兰一家重症监护病房爆发的 CRAb 疫情。在非耐药鲍曼不动杆菌爆发期间，在采取感染控制措施的同时，几个月内从三名患者体内分离出了携带高度相似的 blaNDM-1 和 tet（x3）编码质粒的 CRAb 分离物。利用短读取序列和长读取序列的杂交组合分析了从患者材料中培养出的 CRAb 和不携带碳青霉烯酶的鲍曼尼氏菌分离物的染色体和质粒序列。CRAb分离物显示，CRAb疫情由两种不同的菌株组成，携带相似的质粒。质粒含有多种抗生素耐药基因，包括四环素耐药基因 tet(x3)、blaNDM-1 和 blaOXA-97 碳青霉烯酶基因。我们测定了 13 种抗生素的最小抑菌浓度（MIC），包括新注册的四环素类抗生素克拉维酸和奥美拉唑。除米诺环素的 MIC 值较低外，CRAb 分离物对四环素类抗生素（包括克拉维酸和奥美拉唑）的 MIC 值较高。在这项研究中，我们展示了对耐多药鲍曼不动杆菌进行测序对于追踪疫情和指导疫情缓解措施的价值。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Eurosurveillance INFECTIOUS DISEASES-

CiteScore

32.70

自引率

2.10%

发文量

430

审稿时长

3-8 weeks

期刊介绍： Eurosurveillance is a European peer-reviewed journal focusing on the epidemiology, surveillance, prevention, and control of communicable diseases relevant to Europe.It is a weekly online journal, with 50 issues per year published on Thursdays. The journal includes short rapid communications, in-depth research articles, surveillance reports, reviews, and perspective papers. It excels in timely publication of authoritative papers on ongoing outbreaks or other public health events. Under special circumstances when current events need to be urgently communicated to readers for rapid public health action, e-alerts can be released outside of the regular publishing schedule. Additionally, topical compilations and special issues may be provided in PDF format.