Construction of a bioelectrocatalytic system with bacterial surface displayed enzyme-nanomaterial hybrids

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Bioelectrochemistry Pub Date : 2024-07-08 DOI:10.1016/j.bioelechem.2024.108777

Meiqing Li , Ranran Wu , Haiyan Song , Fei Li , Yuanming Wang , Yu Wang , Lijuan Ma , Zhiguang Zhu

引用次数: 0

Abstract

To take advantage of the high specificity of enzymatic catalysis along with the high efficiency of electrochemical cofactor regeneration, a bacterial surface displayed enzyme-nanomaterial hybrid bioelectrocatalytic system is herein developed. A cofactor-dependent xylose reductase, capable of reducing xylose to xylitol, is displayed on the surface of Bacillus subtilis, followed by the attachment of copper nanomaterials via the binding of His-tagged enzyme with the nickel ion. This hybrid system can regenerate NADPH with a highest efficiency of 71.6% in 4 h without the usage of extra electron mediators, and 2.35 mM of xylitol can be synthesized after a series of optimization processes. This work opens up new possibilities for the construction and application of bioelectrocatalytic systems with enzyme-nanomaterial hybrids.

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利用细菌表面显示的酶-纳米材料混合物构建生物电催化系统。

为了利用酶催化的高特异性和电化学辅助因子再生的高效性，本文开发了一种细菌表面酶-纳米材料混合生物电催化系统。在枯草芽孢杆菌表面展示了一种依赖于辅因子的木糖还原酶，该酶能够将木糖还原成木糖醇，然后通过 His 标记的酶与镍离子的结合附着上纳米铜材料。该混合系统无需使用额外的电子介质，就能在 4 小时内以 71.6% 的最高效率再生 NADPH，并且经过一系列优化过程后可合成 2.35 mM 的木糖醇。这项工作为构建和应用酶-纳米材料混合生物电催化系统提供了新的可能性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bioelectrochemistry 生物-电化学

CiteScore

9.10

自引率

6.00%

发文量

238

审稿时长

38 days

期刊介绍： An International Journal Devoted to Electrochemical Aspects of Biology and Biological Aspects of Electrochemistry Bioelectrochemistry is an international journal devoted to electrochemical principles in biology and biological aspects of electrochemistry. It publishes experimental and theoretical papers dealing with the electrochemical aspects of: • Electrified interfaces (electric double layers, adsorption, electron transfer, protein electrochemistry, basic principles of biosensors, biosensor interfaces and bio-nanosensor design and construction. • Electric and magnetic field effects (field-dependent processes, field interactions with molecules, intramolecular field effects, sensory systems for electric and magnetic fields, molecular and cellular mechanisms) • Bioenergetics and signal transduction (energy conversion, photosynthetic and visual membranes) • Biomembranes and model membranes (thermodynamics and mechanics, membrane transport, electroporation, fusion and insertion) • Electrochemical applications in medicine and biotechnology (drug delivery and gene transfer to cells and tissues, iontophoresis, skin electroporation, injury and repair). • Organization and use of arrays in-vitro and in-vivo, including as part of feedback control. • Electrochemical interrogation of biofilms as generated by microorganisms and tissue reaction associated with medical implants.