Acetylation, ADP-ribosylation and methylation of malate dehydrogenase.

IF 5.6 2区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Essays in biochemistry Pub Date : 2024-10-03 DOI:10.1042/EBC20230080

Misty L Kuhn, John F Rakus, Delphine Quenet

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Abstract

Metabolism within an organism is regulated by various processes, including post-translational modifications (PTMs). These types of chemical modifications alter the molecular, biochemical, and cellular properties of proteins and allow the organism to respond quickly to different environments, energy states, and stresses. Malate dehydrogenase (MDH) is a metabolic enzyme that is conserved in all domains of life and is extensively modified post-translationally. Due to the central role of MDH, its modification can alter metabolic flux, including the Krebs cycle, glycolysis, and lipid and amino acid metabolism. Despite the importance of both MDH and its extensively post-translationally modified landscape, comprehensive characterization of MDH PTMs, and their effects on MDH structure, function, and metabolic flux remains underexplored. Here, we review three types of MDH PTMs - acetylation, ADP-ribosylation, and methylation - and explore what is known in the literature and how these PTMs potentially affect the 3D structure, enzymatic activity, and interactome of MDH. Finally, we briefly discuss the potential involvement of PTMs in the dynamics of metabolons that include MDH.

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苹果酸脱氢酶的乙酰化、ADP-核糖基化和甲基化。

生物体内的新陈代谢受各种过程的调节，包括翻译后修饰（PTM）。这些类型的化学修饰会改变蛋白质的分子、生化和细胞特性，使生物体能够对不同的环境、能量状态和压力做出快速反应。苹果酸脱氢酶（MDH）是一种新陈代谢酶，在生命的所有领域中都是保守的，并被广泛地进行翻译后修饰。由于 MDH 的核心作用，其修饰可改变代谢通量，包括克雷布斯循环、糖酵解以及脂质和氨基酸代谢。尽管 MDH 及其广泛的翻译后修饰景观都很重要，但对 MDH PTMs 的全面描述及其对 MDH 结构、功能和代谢通量的影响仍未得到充分探索。在这里，我们回顾了 MDH 的三种 PTM--乙酰化、ADP-核糖基化和甲基化--并探讨了文献中已知的这些 PTM 如何潜在地影响 MDH 的三维结构、酶活性和相互作用组。最后，我们简要讨论了 PTMs 对包括 MDH 在内的代谢子动态的潜在影响。

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来源期刊

Essays in biochemistry 生物-生化与分子生物学

CiteScore

10.50

自引率

0.00%

发文量

105

审稿时长

>12 weeks

期刊介绍： Essays in Biochemistry publishes short, digestible reviews from experts highlighting recent key topics in biochemistry and the molecular biosciences. Written to be accessible for those not yet immersed in the subject, each article is an up-to-date, self-contained summary of the topic. Bridging the gap between the latest research and established textbooks, Essays in Biochemistry will tell you what you need to know to begin exploring the field, as each article includes the top take-home messages as summary points. Each issue of the journal is guest edited by a key opinion leader in the area, and whether you are continuing your studies or moving into a new research area, the Journal gives a complete picture in one place. Essays in Biochemistry is proud to publish Understanding Biochemistry, an essential online resource for post-16 students, teachers and undergraduates. Providing up-to-date overviews of key concepts in biochemistry and the molecular biosciences, the Understanding Biochemistry issues of Essays in Biochemistry are published annually in October.

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