Can toothbrushing reduce the intraoral viral load of SARS-CoV-2? A pilot study with a dentifrice containing an antimicrobial phthalocyanine derivative.

IF 1.7 Q3 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH GMS Hygiene and Infection Control Pub Date : 2024-06-05 eCollection Date: 2024-01-01 DOI:10.3205/dgkh000487
Marcelo Lupion Poleti, Danielle Gregório, Alisson Gabriel Idelfonso Bistaffa, Fabiano Vieira Vilhena, Andréa Name Colado Simão, Mayara Tiemi Enokida Mori, Nicole Perugini Stadtlober, Marcell Alysson Batisti Lozovoy, Paulo Sérgio da Silva Santos, Berenice Tomoko Tatibana, Thais Maria Freire Fernandes
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Abstract

The aim of this study was to assess whether toothbrushing with a dentifrice containing an antimicrobial phthalocyanine derivative (APD) can reduce the intraoral viral load of SARS-CoV-2. Twenty COVID-19-positive dentate patients aged ≥18 years were selected instructed to brush their teeth for 2 min with a dentifrice containing APD. Self-collected samples of unstimulated saliva were carried out three times: T0 (baseline), T5 (5 min after toothbrushing), and T30 (30 min after toothbrushing). The analysis of viral RNA was performed by RT-qPCR for detection of three viral genes (ORF1ab, N and S genes). Results were statistically tested using Friedman's test and pairwise comparison with Bonferroni corrections, with a significance level of 5%. There was an increase in the cycle threshold (Ct) value from T0 to T5 in 13 patients (72.2%), and from T0 to T30 in 14 patients (77.8%). In two patients (11.1%) no SARS-CoV-2 was detected at T5 and five patients (27.8%) at T30. The Ct values were statistically significantly higher (p=0.020) at T30 in comparison to T0 and T5. This pilot study suggests that toothbrushing with a dentifrice containing APD could reduce the SARS-CoV-2 viral load in the oral cavity. However, further studies are needed to confirm this possible beneficial effect against SARS-CoV-2.

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刷牙能减少 SARS-CoV-2 的口腔内病毒载量吗?使用含有抗菌酞菁衍生物的牙膏进行试点研究。
本研究的目的是评估使用含有抗菌酞菁衍生物(APD)的牙膏刷牙是否能减少 SARS-CoV-2 的口腔内病毒载量。选取了 20 名 COVID-19 阳性、年龄≥18 岁的无牙患者,指导他们使用含 APD 的牙膏刷牙 2 分钟。对未受刺激的唾液样本进行三次自我采集:T0(基线)、T5(刷牙后 5 分钟)和 T30(刷牙后 30 分钟)。病毒 RNA 分析采用 RT-qPCR 方法,检测三个病毒基因(ORF1ab、N 和 S 基因)。结果采用弗里德曼检验和配对比较进行统计检验,并进行 Bonferroni 校正,显著性水平为 5%。13名患者(72.2%)的周期阈值(Ct)从T0升至T5,14名患者(77.8%)的周期阈值(Ct)从T0升至T30。有 2 名患者(11.1%)在 T5 检测不到 SARS-CoV-2,有 5 名患者(27.8%)在 T30 检测不到 SARS-CoV-2。与 T0 和 T5 相比,T30 的 Ct 值在统计学上明显更高(p=0.020)。这项试验研究表明,使用含 APD 的牙膏刷牙可减少口腔中的 SARS-CoV-2 病毒载量。然而,还需要进一步的研究来证实这种可能对 SARS-CoV-2 有益的效果。
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GMS Hygiene and Infection Control
GMS Hygiene and Infection Control PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH-
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审稿时长
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