Highly efficient capture of Escherichia coli using chitosan-lysozyme modified nanofiber membranes: Potential applications in food packaging and water treatment
Thi Tam An Tran , Edouard Gnoumou , Bing-Lan Liu , Penjit Srinophakun , Chen‑Yaw Chiu , Chi-Yun Wang , Kuei-Hsiang Chen , Yu-Kaung Chang
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引用次数: 0
Abstract
Functionalized nanofiber membranes play a vital role in water treatment by effectively removing pollutants such as heavy metals and dyes. However, microbial contamination remains a significant challenge. This study created a novel antibacterial nanofiber membrane by grafting chitosan (CS) and lysozyme (LYZ) onto a P-COOH weak ion exchange nanofiber membrane. The physical features of the nanofiber membranes were studied, and the immobilization of CS and LYZ onto P-COOH nanofiber membranes was examined to improve Escherichia coli (E. coli) killing. The membrane exhibited impressive antibacterial properties, achieving approximately 100 % efficacy against E. coli. It maintained high effectiveness even after reuse and storage, with 98.71 % efficacy after five cycles of reuse. Cytotoxicity tests confirmed high biocompatibility with a 100 % viability rate. The innovation of this study lies in the grafting of CS and LYZ onto P-COOH weak ion exchange nanofiber membranes, resulting in a highly efficient antibacterial composite. The P-COOH-CS-LYZ membrane exhibits superior antibacterial efficacy and maintains its high effectiveness after repeated reuse and long-term storage, underscoring its durability and practical applicability. Additionally, its high biocompatibility and sustainable design, using natural and non-toxic agents, represent a significant advancement in eco-friendly water treatment technologies. This dual-functional membrane effectively addresses microbial contamination and pollutant removal, offering a comprehensive solution for water treatment.
期刊介绍:
The Biochemical Engineering Journal aims to promote progress in the crucial chemical engineering aspects of the development of biological processes associated with everything from raw materials preparation to product recovery relevant to industries as diverse as medical/healthcare, industrial biotechnology, and environmental biotechnology.
The Journal welcomes full length original research papers, short communications, and review papers* in the following research fields:
Biocatalysis (enzyme or microbial) and biotransformations, including immobilized biocatalyst preparation and kinetics
Biosensors and Biodevices including biofabrication and novel fuel cell development
Bioseparations including scale-up and protein refolding/renaturation
Environmental Bioengineering including bioconversion, bioremediation, and microbial fuel cells
Bioreactor Systems including characterization, optimization and scale-up
Bioresources and Biorefinery Engineering including biomass conversion, biofuels, bioenergy, and optimization
Industrial Biotechnology including specialty chemicals, platform chemicals and neutraceuticals
Biomaterials and Tissue Engineering including bioartificial organs, cell encapsulation, and controlled release
Cell Culture Engineering (plant, animal or insect cells) including viral vectors, monoclonal antibodies, recombinant proteins, vaccines, and secondary metabolites
Cell Therapies and Stem Cells including pluripotent, mesenchymal and hematopoietic stem cells; immunotherapies; tissue-specific differentiation; and cryopreservation
Metabolic Engineering, Systems and Synthetic Biology including OMICS, bioinformatics, in silico biology, and metabolic flux analysis
Protein Engineering including enzyme engineering and directed evolution.