Ping Yang , Tingting Yao , Xinyi Liu , Aimin Zhang , Jian Zhang , Lina Pang
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引用次数: 0
Abstract
The strain Ralstonia pickettii J4 was isolated as a novel heterotrophic nitrification - aerobic denitrification (HN-AD) and its potential in removing nitrogen was assessed. Results indicated that the optimal HN-AD efficiencies by strain J4 were 98.99 % for ammonium nitrogen (NH4+-N) and 88.86 % for total nitrogen, when using glucose as the optimal carbon source at 30 °C and under conditions of carbon/nitrogen ratio of 15, pH of 7, shaking speed of 100 rpm, and initial NH4+-N concentration of 50 mg/L. Analysis results of nitrogen balance and functional genes revealed the possible HN-AD pathway of J4: NH4+-N→ hydroxylamine→ nitrite nitrogen→ nitric oxide→ nitrous oxide→ nitrogen gas, without nitrite accumulation. Strain J4 significantly enhanced the nitrogen removal performance of a sequencing biofilm batch reactor by enriching the functional bacterial community involved in nitrogen removal, for instance, Thauera, Hydrogenophaga, and Flavobacterium. These findings are important for providing bioaugmentation sources regarding nitrogen removal of wastewater treatment.
期刊介绍:
The Biochemical Engineering Journal aims to promote progress in the crucial chemical engineering aspects of the development of biological processes associated with everything from raw materials preparation to product recovery relevant to industries as diverse as medical/healthcare, industrial biotechnology, and environmental biotechnology.
The Journal welcomes full length original research papers, short communications, and review papers* in the following research fields:
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Environmental Bioengineering including bioconversion, bioremediation, and microbial fuel cells
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Bioresources and Biorefinery Engineering including biomass conversion, biofuels, bioenergy, and optimization
Industrial Biotechnology including specialty chemicals, platform chemicals and neutraceuticals
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Metabolic Engineering, Systems and Synthetic Biology including OMICS, bioinformatics, in silico biology, and metabolic flux analysis
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