Efficient heterotrophic nitrification – aerobic denitrification by a novel bacterium Ralstonia pickettii J4: Isolation, identification, and application

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biochemical Engineering Journal Pub Date : 2024-07-06 DOI:10.1016/j.bej.2024.109417

Ping Yang , Tingting Yao , Xinyi Liu , Aimin Zhang , Jian Zhang , Lina Pang

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Abstract

The strain Ralstonia pickettii J4 was isolated as a novel heterotrophic nitrification - aerobic denitrification (HN-AD) and its potential in removing nitrogen was assessed. Results indicated that the optimal HN-AD efficiencies by strain J4 were 98.99 % for ammonium nitrogen (NH₄⁺-N) and 88.86 % for total nitrogen, when using glucose as the optimal carbon source at 30 °C and under conditions of carbon/nitrogen ratio of 15, pH of 7, shaking speed of 100 rpm, and initial NH₄⁺-N concentration of 50 mg/L. Analysis results of nitrogen balance and functional genes revealed the possible HN-AD pathway of J4: NH₄⁺-N→ hydroxylamine→ nitrite nitrogen→ nitric oxide→ nitrous oxide→ nitrogen gas, without nitrite accumulation. Strain J4 significantly enhanced the nitrogen removal performance of a sequencing biofilm batch reactor by enriching the functional bacterial community involved in nitrogen removal, for instance, Thauera, Hydrogenophaga, and Flavobacterium. These findings are important for providing bioaugmentation sources regarding nitrogen removal of wastewater treatment.

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新型细菌 Ralstonia pickettii J4 的高效异养硝化-好氧反硝化作用：分离、鉴定和应用

研究人员分离了新型异养硝化-好氧脱氮（HN-AD）菌株 Ralstonia pickettii J4，并对其脱氮潜力进行了评估。结果表明，在 30 ℃、碳氮比为 15、pH 值为 7、振荡速度为 100 rpm、初始 NH4+-N 浓度为 50 mg/L 的条件下，以葡萄糖为最佳碳源时，菌株 J4 的最佳 HN-AD 去除铵态氮（NH4+-N）的效率为 98.99%，去除总氮的效率为 88.86%。氮平衡和功能基因分析结果表明，J4菌株可能的HN-AD途径为：NH4+-N→羟胺→亚硝酸盐氮→一氧化氮→一氧化二氮→氮气，无亚硝酸盐积累。菌株 J4 通过丰富参与脱氮的功能细菌群落，如 Thauera、Hydrogenophaga 和 Flavobacterium，大大提高了测序生物膜批式反应器的脱氮性能。这些发现对于为废水处理脱氮提供生物增强源非常重要。

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来源期刊

Biochemical Engineering Journal 工程技术-工程：化工

CiteScore

7.10

自引率

5.10%

发文量

380

审稿时长

34 days

期刊介绍： The Biochemical Engineering Journal aims to promote progress in the crucial chemical engineering aspects of the development of biological processes associated with everything from raw materials preparation to product recovery relevant to industries as diverse as medical/healthcare, industrial biotechnology, and environmental biotechnology. The Journal welcomes full length original research papers, short communications, and review papers* in the following research fields: Biocatalysis (enzyme or microbial) and biotransformations, including immobilized biocatalyst preparation and kinetics Biosensors and Biodevices including biofabrication and novel fuel cell development Bioseparations including scale-up and protein refolding/renaturation Environmental Bioengineering including bioconversion, bioremediation, and microbial fuel cells Bioreactor Systems including characterization, optimization and scale-up Bioresources and Biorefinery Engineering including biomass conversion, biofuels, bioenergy, and optimization Industrial Biotechnology including specialty chemicals, platform chemicals and neutraceuticals Biomaterials and Tissue Engineering including bioartificial organs, cell encapsulation, and controlled release Cell Culture Engineering (plant, animal or insect cells) including viral vectors, monoclonal antibodies, recombinant proteins, vaccines, and secondary metabolites Cell Therapies and Stem Cells including pluripotent, mesenchymal and hematopoietic stem cells; immunotherapies; tissue-specific differentiation; and cryopreservation Metabolic Engineering, Systems and Synthetic Biology including OMICS, bioinformatics, in silico biology, and metabolic flux analysis Protein Engineering including enzyme engineering and directed evolution.