Implications of high PsSAD expression and oleic acid content in defining the Sujata phenotype of Papaver somniferum

IF 5.4 Q1 PLANT SCIENCES Current Plant Biology Pub Date : 2024-06-30 DOI:10.1016/j.cpb.2024.100366
Mridula Singh , Shiv S. Pandey , Sucheta Singh , Prasant K. Rout , Abdul Samad , Chandan S. Chanotiya , Alok Kalra , Ashutosh K. Shukla
{"title":"Implications of high PsSAD expression and oleic acid content in defining the Sujata phenotype of Papaver somniferum","authors":"Mridula Singh ,&nbsp;Shiv S. Pandey ,&nbsp;Sucheta Singh ,&nbsp;Prasant K. Rout ,&nbsp;Abdul Samad ,&nbsp;Chandan S. Chanotiya ,&nbsp;Alok Kalra ,&nbsp;Ashutosh K. Shukla","doi":"10.1016/j.cpb.2024.100366","DOIUrl":null,"url":null,"abstract":"<div><p><em>Papaver somniferum</em> L. produces therapeutically-useful alkaloids like morphine, codeine, papaverine, and thebaine that accumulate in the latex of its capsule. Apart from its alkaloids, the edible seeds of the plant have high nutritional value and culinary use, with the seed oil being rich in the health-promoting unsaturated fatty acids. Sujata is low alkaloid-producing latex-less culinary variety of <em>P. somniferum</em> developed from an alkaloid-rich gum harvest parent (Sampada) for curtailing the narcotic menace of morphine and opium (dried latex). Earlier, it has been shown that the expression of stearoyl-acyl-carrier protein desaturase (<em>PsSAD</em>) in the young capsule and the proportion of unsaturated fatty acids in the seeds are higher in Sujata as compared to Sampada. Here, we studied bacterial endophytes isolated from Sujata tissues for their role in defining the fatty acid (saturated stearic versus unsaturated oleic) profile in its leaves and seeds. Besides, a potyvirus infecting <em>P. somniferum</em> was characterized through its coat protein gene sequencing and transmission electron microscopy and the two genotypes (Sujata and Sampada) contrasting in their phenotypes (oil/fatty acid, alkaloid and latex profiles) were compared for their susceptibility towards it through indicator plant- and qRT-PCR-based virus infectivity assays. In both the assays, Sujata was found to have higher susceptibility to the potyvirus, as compared to Sampada. The most plausible reason for this could be the higher <em>PsSAD</em> expression in the leaves of Sujata, which resulted in higher relative levels of oleic acid and lower relative levels of stearic acid in its leaves as compared to that of Sampada.</p></div>","PeriodicalId":38090,"journal":{"name":"Current Plant Biology","volume":"39 ","pages":"Article 100366"},"PeriodicalIF":5.4000,"publicationDate":"2024-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2214662824000483/pdfft?md5=47e089a62e51960b51d0a9e177c81807&pid=1-s2.0-S2214662824000483-main.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Plant Biology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2214662824000483","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0

Abstract

Papaver somniferum L. produces therapeutically-useful alkaloids like morphine, codeine, papaverine, and thebaine that accumulate in the latex of its capsule. Apart from its alkaloids, the edible seeds of the plant have high nutritional value and culinary use, with the seed oil being rich in the health-promoting unsaturated fatty acids. Sujata is low alkaloid-producing latex-less culinary variety of P. somniferum developed from an alkaloid-rich gum harvest parent (Sampada) for curtailing the narcotic menace of morphine and opium (dried latex). Earlier, it has been shown that the expression of stearoyl-acyl-carrier protein desaturase (PsSAD) in the young capsule and the proportion of unsaturated fatty acids in the seeds are higher in Sujata as compared to Sampada. Here, we studied bacterial endophytes isolated from Sujata tissues for their role in defining the fatty acid (saturated stearic versus unsaturated oleic) profile in its leaves and seeds. Besides, a potyvirus infecting P. somniferum was characterized through its coat protein gene sequencing and transmission electron microscopy and the two genotypes (Sujata and Sampada) contrasting in their phenotypes (oil/fatty acid, alkaloid and latex profiles) were compared for their susceptibility towards it through indicator plant- and qRT-PCR-based virus infectivity assays. In both the assays, Sujata was found to have higher susceptibility to the potyvirus, as compared to Sampada. The most plausible reason for this could be the higher PsSAD expression in the leaves of Sujata, which resulted in higher relative levels of oleic acid and lower relative levels of stearic acid in its leaves as compared to that of Sampada.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
高 PsSAD 表达和油酸含量对确定巴布亚草 Sujata 表型的影响
罂粟碱(Papaver somniferum L.)可产生吗啡、可待因、罂粟碱和蒂巴因等对治疗有用的生物碱,这些生物碱积聚在其蒴果的胶乳中。除生物碱外,该植物的可食用种子具有很高的营养价值和烹饪用途,种子油富含促进健康的不饱和脂肪酸。Sujata 是一种生物碱产量低、不含乳汁的索门冬食用品种,由富含生物碱的胶质收获母本(Sampada)发展而来,用于遏制吗啡和鸦片(干乳汁)的麻醉威胁。早先的研究表明,与 Sampada 相比,Sujata 的幼蒴果中硬脂酰-酰-载体蛋白去饱和酶(PsSAD)的表达量和种子中不饱和脂肪酸的比例更高。在此,我们研究了从 Sujata 组织中分离出来的细菌内生菌在确定其叶片和种子中脂肪酸(饱和硬脂酸与不饱和油酸)组成方面的作用。此外,还通过衣壳蛋白基因测序和透射电子显微镜鉴定了一种感染索姆尼弗兰的钾病毒,并通过指示植物和基于 qRT-PCR 的病毒感染性检测比较了两种基因型(Sujata 和 Sampada)在表型(油/脂肪酸、生物碱和乳胶特征)上的对比。在这两项检测中,都发现 Sujata 与 Sampada 相比,对 potyvirus 的敏感性更高。最合理的原因可能是 Sujata 的叶片中 PsSAD 表达量较高,导致其叶片中油酸的相对含量比 Sampada 高,硬脂酸的相对含量比 Sampada 低。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Current Plant Biology
Current Plant Biology Agricultural and Biological Sciences-Plant Science
CiteScore
10.90
自引率
1.90%
发文量
32
审稿时长
50 days
期刊介绍: Current Plant Biology aims to acknowledge and encourage interdisciplinary research in fundamental plant sciences with scope to address crop improvement, biodiversity, nutrition and human health. It publishes review articles, original research papers, method papers and short articles in plant research fields, such as systems biology, cell biology, genetics, epigenetics, mathematical modeling, signal transduction, plant-microbe interactions, synthetic biology, developmental biology, biochemistry, molecular biology, physiology, biotechnologies, bioinformatics and plant genomic resources.
期刊最新文献
Sulfur redirects carbon metabolism to optimize nitrogen utilization and promote andrographolide biosynthesis in Andrographis paniculata seedlings Advancing sustainability: The impact of emerging technologies in agriculture Rock dust-based potting media enhances agronomic performance and nutritional quality of horticultural crops Probing marine macroalgal phlorotannins as an antibacterial candidate against Salmonella typhi: Molecular docking and dynamics simulation approach Integrated transcriptomic and metabolomic analysis reveals the effects of forchlorfenuron and thidiazuron on flavonoid biosynthesis in table grape skins
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1