Proteome overview of exosome derived from plasma of cows infected with Mycobacterium bovis

IF 2.8 3区医学 Q3 IMMUNOLOGY Tuberculosis Pub Date : 2024-07-06 DOI:10.1016/j.tube.2024.102541

Hangfan Zhou , Wenhui Wu , Qilong Zhang , Tao Zhang , Songhao Jiang , Lei Chang , Yuping Xie , Jiaqiang Zhu , Degang Zhou , Yao Zhang , Ping Xu

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Abstract

Bovine tuberculosis (bTB), primarily caused by Mycobacterium bovis (M. bovis), is a globally zoonotic disease with significant economic impacts. Plasma exosomes have been extensively used for investigating disease processes and exploring biomarkers. While mass spectrometry (MS)-based proteomic analysis of plasma exosomes has been employed for human tuberculosis (TB) studies, it has not yet been applied to bTB. Therefore, a comprehensive proteomic overview of plasma exosomes from M. bovis-infected cows is essential. In this study, we presented an extensive proteomic analysis of plasma exosomes from 89 M. bovis-infected cows across three farms, using data dependent acquisition (DDA) mode. Our analysis encompasses 239,894 spectra, 6,011 peptides and 835 proteins. The proteomic overview revealed both consistencies and differences among individual cows, supplements 595 proteins to the bovine exosome library, and enriches tuberculosis and related pathways. Additionally, six pathways were validated as immune response pathways, and three proteins (CATHL1, H1-1, and LCN2) were identified as potential indicators of bTB. This study is the first to investigate the exosome proteome of plasma from cows infected with M. bovis, providing a valuable dataset for exploring candidate bTB markers and understanding the mechanisms of host defense against M. bovis.

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从感染了牛分枝杆菌的奶牛血浆中提取的外泌体蛋白质组概览。

牛结核病（bTB）主要由牛分枝杆菌（M. bovis）引起，是一种全球性的人畜共患疾病，对经济造成重大影响。血浆外泌体已被广泛用于研究疾病过程和探索生物标记物。虽然基于质谱（MS）的血浆外泌体蛋白质组学分析已用于人类结核病（TB）研究，但尚未应用于牛结核病。因此，对受牛结核杆菌感染的奶牛血浆外泌体进行全面的蛋白质组学研究至关重要。在本研究中，我们采用数据依赖采集（DDA）模式，对来自三个牧场的89头牛M.感染奶牛的血浆外泌体进行了广泛的蛋白质组学分析。我们的分析包括 239,894 个光谱、6,011 个肽段和 835 个蛋白质。蛋白质组概述揭示了不同奶牛之间的一致性和差异性，为牛外泌体文库补充了 595 种蛋白质，并丰富了结核病和相关途径。此外，六条通路被验证为免疫反应通路，三种蛋白质（CATHL1、H1-1 和 LCN2）被确定为结核病的潜在指标。这项研究首次调查了感染牛海绵状芽孢杆菌的奶牛血浆的外泌体蛋白质组，为探索候选牛结核标记物和了解宿主防御牛海绵状芽孢杆菌的机制提供了宝贵的数据集。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Tuberculosis 医学-呼吸系统

CiteScore

4.60

自引率

3.10%

发文量

审稿时长

49 days

期刊介绍： Tuberculosis is a speciality journal focusing on basic experimental research on tuberculosis, notably on bacteriological, immunological and pathogenesis aspects of the disease. The journal publishes original research and reviews on the host response and immunology of tuberculosis and the molecular biology, genetics and physiology of the organism, however discourages submissions with a meta-analytical focus (for example, articles based on searches of published articles in public electronic databases, especially where there is lack of evidence of the personal involvement of authors in the generation of such material). We do not publish Clinical Case-Studies. Areas on which submissions are welcomed include: -Clinical TrialsDiagnostics- Antimicrobial resistance- Immunology- Leprosy- Microbiology, including microbial physiology- Molecular epidemiology- Non-tuberculous Mycobacteria- Pathogenesis- Pathology- Vaccine development. This Journal does not accept case-reports. The resurgence of interest in tuberculosis has accelerated the pace of relevant research and Tuberculosis has grown with it, as the only journal dedicated to experimental biomedical research in tuberculosis.