Utility of p53 in assessing margin status of resected squamous cell carcinoma

Abstract

Introduction

Local recurrence of squamous cell carcinoma (SCC) occurs despite the report of negative histologic margins. P53 immunohistochemistry (IHC) has been recently shown to be able identify high-risk dysplasia that otherwise displays low-grade morphology. This study seeks to apply p53 IHC in evaluating a cohort of resected oral SCC and evaluate its utility in assessing margin status.

Materials and Methods

Cases of SCC, resected at our institution, with known p53 mutations were identified between 2015-2020. Data from the chart and pathology reports were reviewed. Blocks were pulled if available and additional H&E and p53 IHC was performed on the main tumor and margins. Slides were assessed for the presence of dysplasia and p53 staining pattern.

Results

At time of abstract submission, 5 patient cases were identified meeting inclusion criteria and had available tissue. P53 status will be reported. Issues with laser and frozen section artifact were identified. Further analysis is ongoing.

Conclusions

We report the results of a study applying p53 IHC in evaluating the margins of resected SCC. These findings will provide the basis for initiating a larger study investigating the reliability and prognostic implications of p53 staining patterns in SCC.