BCL-2 and Ki-67 expression in Odontogenic Keratocysts

IF 2 3区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Oral Surgery Oral Medicine Oral Pathology Oral Radiology Pub Date : 2024-07-13 DOI:10.1016/j.oooo.2024.04.024
Dr. Joud Y. Omari, Dr. Min Kyeong Kim, Prof. Sook-Bin Woo
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引用次数: 0

Abstract

Introduction

Odontogenic keratocyst/keratocystic odontogenic tumor (OKC/KCOT) is a cystic odontogenic neoplasm that harbors PTCH1 gene mutation in 93% of the cases. BCL-2 is an anti-apoptotic gene that functions through the regulation of Ca 2 concentration, maintenance of mitochondrial integrity and promoting cell survival during the G0 of cell cycle when growth factors are depleted. It is expressed in some benign neoplasms such as spindle cell lipoma and solitary fibrous tumor.

Aim

We aim to investigate the expression of BCL-2 and Ki-67 in OKC/KCOT compared to other odontogenic cysts and tumors.

Methods

26 cases of sporadic OKC/KCOT and 24 controls, including 15 dentigerous cysts, nine other odontogenic cysts and three cases of unicystic ameloblastoma were stained for BCL-2 and Ki-67.

Results

The epithelium exhibited dysmaturation in >1/3 of the epithelium in 15 (57.7%) of the cases. All cases (100%) were positive for BCL-2 involving basal cell cytoplasm with 15 cases (57.7%) involving up to one third of the epithelium, and 3 (11.5%) involving more than one-third of the epithelium. Expression of BCL-2 was completely lost in areas where the epithelium was inflamed. Ki-67 showed >30% expression in 19 cases at least focally while the rest showed 5-30% positivity. Ki-67 expression was significantly higher in cases that exhibited dysmaturation in >1/3 of the epithelium (p= 0.032). None of the cyst controls were positive for BCL-2 expression. All three cases of unicystic ameloblastoma were diffusely positive for BCL-2.

Conclusion

BCL-2 is a sensitive marker for OKC/KCOT staining the basal cell cytoplasm in 100% of cases. It is useful in identifying cases with histologic features suggestive of but not definitive for OKC/KCOT especially in small specimens, as well as for excluding this diagnosis in cysts that exhibit focal parakeratosis without other features of OKC/KCOT.

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牙源性角化囊肿中 BCL-2 和 Ki-67 的表达
导言牙源性角化囊肿/角化囊性牙源性肿瘤(OKC/KCOT)是一种囊性牙源性肿瘤,93%的病例存在 PTCH1 基因突变。BCL-2 是一种抗凋亡基因,通过调节 Ca 2 浓度、维持线粒体完整性以及在细胞周期 G0 期生长因子耗竭时促进细胞存活来发挥作用。我们的目的是研究 BCL-2 和 Ki-67 在 OKC/KCOT 与其他牙源性囊肿和肿瘤中的表达情况。方法对 26 例散发性 OKC/KCOT 和 24 例对照病例(包括 15 例牙源性囊肿、9 例其他牙源性囊肿和 3 例单囊性釉母细胞瘤)进行 BCL-2 和 Ki-67 染色。所有病例(100%)的基底细胞胞浆中BCL-2均呈阳性,其中15例(57.7%)涉及最多三分之一的上皮,3例(11.5%)涉及超过三分之一的上皮。在上皮发炎的区域,BCL-2的表达完全消失。Ki-67在19个病例中至少有30%的表达,其余病例的阳性率为5%-30%。1/3上皮发育不良的病例中,Ki-67的表达明显更高(p= 0.032)。没有一个囊肿对照组的 BCL-2 表达呈阳性。结论BCL-2是一种敏感的OKC/KCOT标记物,可对100%的病例的基底细胞胞质进行染色。它有助于鉴别具有组织学特征提示但不能确定为 OKC/KCOT 的病例,尤其是在小标本中,也有助于在表现为局灶性角化不全但无其他 OKC/KCOT 特征的囊肿中排除这一诊断。
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来源期刊
Oral Surgery Oral Medicine Oral Pathology Oral Radiology
Oral Surgery Oral Medicine Oral Pathology Oral Radiology DENTISTRY, ORAL SURGERY & MEDICINE-
CiteScore
3.80
自引率
6.90%
发文量
1217
审稿时长
2-4 weeks
期刊介绍: Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology is required reading for anyone in the fields of oral surgery, oral medicine, oral pathology, oral radiology or advanced general practice dentistry. It is the only major dental journal that provides a practical and complete overview of the medical and surgical techniques of dental practice in four areas. Topics covered include such current issues as dental implants, treatment of HIV-infected patients, and evaluation and treatment of TMJ disorders. The official publication for nine societies, the Journal is recommended for initial purchase in the Brandon Hill study, Selected List of Books and Journals for the Small Medical Library.
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