Parallel phenotypic evolution of two independent cavefish lineages of Astyanax mexicanus (De Filippi, 1854) (Characiformes: Characidae)

Abstract

Astyanax mexicanus offers a unique model for studying parallel evolution, with cave-dwelling forms arising from at least two distinct surface lineages. Here, we examined repeated evolution in the two cave-adapted lineages across four phenotypic datasets: (i) meristic data; (ii) ecomorphological data; and geometric morphometric data for (iii) head and (iv) premaxillary bone shape. Additionally, we assessed the association of these phenotypes with their phylogenetic history. Certain traits exhibited evidence of parallel evolution for the cave ecotype across lineages, such as increased maxillary tooth count, reduced lateral line scale count, and shorter ascending process of the premaxillary bone. Geometric morphometric data showed a phenotypic divergence gradient in head shape between cave and surface populations, with the strongest divergence between Sierra de El Abra and surface populations and with less differentiation between surface populations and caves from Sierra de Guatemala and Micos. Cave populations that hybridize with surface populations (e.g. Micos) displayed the greatest overlap with the surface ecotype. There was a similar divergence gradient for premaxillary bone shape, but with weaker differentiation between cave and surface ecotypes. This evidence of repeated evolution in several cave traits emphasizes the significance of selective pressures exerted by the environment on the evolution of cave ecotypes.