Fungal Enzymes for Saccharification of Gamma-Valerolactone-Pretreated White Birch Wood: Optimization of the Production of Talaromyces amestolkiae Cellulolytic Cocktail

IF 3.9 4区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Engineering in Life Sciences Pub Date : 2024-07-11 DOI:10.1002/elsc.202400029
Laura I. de Eugenio, Isabel de la Torre, Felipe de Salas, Francisco Vila, David Alonso, Alicia Prieto, María Jesús Martínez
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Abstract

Lignocellulosic biomass, the most abundant natural resource on earth, can be used for cellulosic ethanol production but requires a pretreatment to improve enzyme access to the polymeric sugars while obtaining value from the other components. γ-Valerolactone (GVL) is a promising candidate for biomass pretreatment since it is renewable and bio-based. In the present work, the effect of a pretreatment based on GVL on the enzymatic saccharification of white birch was evaluated at a laboratory scale and the importance of the washing procedure for the subsequent saccharification was demonstrated. Both the saccharification yield and the production of cellulosic ethanol were higher using a noncommercial enzyme crude from Talaromyces amestolkiae than with the commercial cocktail Cellic CTec2 from Novozymes. Furthermore, the production of extracellular cellulases by T. amestolkiae has been optimized in 2 L bioreactors, with improvements ranging from 40% to 75%. Finally, it was corroborated by isoelectric focus that optimization of cellulase secretion by T. amestolkiae did not affect the pattern production of the main β-glucosidases and endoglucanases secreted by this fungus.

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用于γ-戊内酯预处理白桦木糖化的真菌酵素:塔拉酵母菌纤维素分解鸡尾酒的优化生产
木质纤维素生物质是地球上最丰富的自然资源,可用于生产纤维素乙醇,但需要进行预处理,以提高酶对聚合糖的利用率,同时从其他成分中获取价值。γ-戊内酯(GVL)是生物质预处理的理想候选物质,因为它是可再生的生物基。本研究在实验室规模上评估了基于 GVL 的预处理对白桦木酶法糖化的影响,并证明了洗涤程序对后续糖化的重要性。与诺维信公司的商品鸡尾酒 Cellic CTec2 相比,使用非商业性的羊角酵母菌酶粗品的糖化率和纤维素乙醇产量都更高。此外,在 2 升的生物反应器中,对羊角酵母菌生产胞外纤维素酶进行了优化,改进幅度在 40% 至 75% 之间。最后,等电聚焦法证实,优化 T. amestolkiae 的纤维素酶分泌并不影响该真菌分泌的主要 β-葡萄糖苷酶和内切葡聚糖酶的生产模式。
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来源期刊
Engineering in Life Sciences
Engineering in Life Sciences 工程技术-生物工程与应用微生物
CiteScore
6.40
自引率
3.70%
发文量
81
审稿时长
3 months
期刊介绍: Engineering in Life Sciences (ELS) focuses on engineering principles and innovations in life sciences and biotechnology. Life sciences and biotechnology covered in ELS encompass the use of biomolecules (e.g. proteins/enzymes), cells (microbial, plant and mammalian origins) and biomaterials for biosynthesis, biotransformation, cell-based treatment and bio-based solutions in industrial and pharmaceutical biotechnologies as well as in biomedicine. ELS especially aims to promote interdisciplinary collaborations among biologists, biotechnologists and engineers for quantitative understanding and holistic engineering (design-built-test) of biological parts and processes in the different application areas.
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