Application of pulsed-field gel electrophoresis for molecular identification of pathogenic Leptospira species in Iran: a rapid and reliable method.

IF 1.3 Q4 MICROBIOLOGY Iranian Journal of Microbiology Pub Date : 2024-06-01 DOI:10.18502/ijm.v16i3.15763

Pejvak Khaki, Mohsen Bagherpour, Mehdi Gharakhani, Maryam Sadat Soltani, Fereshteh Shahcheraghi, Vajihe Sadat Nikbin

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引用次数: 0

Abstract

Background and objectives: Leptospirosis is a zoonotic disease caused by pathogenic Leptospira serovars. The genus Leptospira cannot differentiated by conventional techniques. However, identity determination of pathogenic serovar is precious of public health problems and epidemiological studies. Pulsed-field gel electrophoresis facilitates rapid identification of Leptospires to the serovar levels.

Materials and methods: In this study, we employed PFGE to evaluate 28 Leptospira isolates, with animal, human and environmental origin, obtained from Razi Vaccine and Serum Research Institute of Karaj, Iran. PFGE patterns of 28 Leptospira serovars were generated using the Not I restriction enzyme in comparison with the lambda ladder.

Results: Out of 28 serovars evaluated, we identified 22 different pulsed types, designated P1-P22. Out of 22 pulse groups, 3 were found to be a common type, but others were a single Type. Groups consisting of the common type were P3, P9, P14, and P16. The results showed that the discriminatory index of PFGE by Not I enzyme was 0.99, demonstrating heterogeneous differentiation among serovar members.

Conclusion: The PFGE methodology used in this study showed excellent interlaboratory report usability, rapid, reliable, enabling standardization and data sharing between laboratories.

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应用脉冲场凝胶电泳对伊朗的病原钩端螺旋体物种进行分子鉴定：一种快速可靠的方法。

背景和目的：钩端螺旋体病是一种由致病性钩端螺旋体血清型引起的人畜共患疾病。传统技术无法区分钩端螺旋体属。然而，确定致病性血清型是公共卫生问题和流行病学研究的关键。脉冲场凝胶电泳有助于快速鉴定钩端螺旋体的血清型：在这项研究中，我们采用脉冲场凝胶电泳对从伊朗卡拉季的拉齐疫苗和血清研究所分离的 28 株钩端螺旋体进行了评估，这些钩端螺旋体来自动物、人类和环境。使用 Not I 限制酶与 lambda 梯形图进行比较，得出了 28 个钩端螺旋体血清型的 PFGE 模式：结果：在所评估的 28 个血清型中，我们确定了 22 个不同的脉冲类型，命名为 P1-P22。在 22 个脉冲群中，发现有 3 个是共同类型，其他则是单一类型。由共同类型组成的组别为 P3、P9、P14 和 P16。结果显示，Not I 酶的 PFGE 鉴别指数为 0.99，表明血清成员之间存在异质性区分：结论：本研究中使用的 PFGE 方法在实验室间报告的可用性方面表现出色，快速、可靠，可实现实验室间的标准化和数据共享。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Iranian Journal of Microbiology MICROBIOLOGY-

CiteScore

2.40

自引率

7.10%

发文量

审稿时长

12 weeks

期刊介绍： The Iranian Journal of Microbiology (IJM) is an international, multi-disciplinary, peer-reviewed journal that provides rapid publication of the most advanced scientific research in the areas of basic and applied research on bacteria and other micro-organisms, including bacteria, viruses, yeasts, fungi, microalgae, and protozoa concerning the development of tools for diagnosis and disease control, epidemiology, antimicrobial agents, clinical microbiology, immunology, Genetics, Genomics and Molecular Biology. Contributions may be in the form of original research papers, review articles, short communications, case reports, technical reports, and letters to the Editor. Research findings must be novel and the original data must be available for review by the Editors, if necessary. Studies that are preliminary, of weak originality or merely descriptive as well as negative results are not appropriate for the journal. Papers considered for publication must be unpublished work (except in an abstract form) that is not under consideration for publication anywhere else, and all co-authors should have agreed to the submission. Manuscripts should be written in English.