Application of ELISA in Cultural Heritage: Recent Advances and Challenges.

IF 4.2 2区 化学 Q1 CHEMISTRY, ANALYTICAL Critical reviews in analytical chemistry Pub Date : 2024-07-16 DOI:10.1080/10408347.2024.2379853
Yufan Zhang, Qiang Li, Bingjian Zhang
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Abstract

Organic residue analyses have long been the primary focus and challenge in the fields of scientific archaeology and cultural heritage. Enzyme-linked immunosorbent assay (ELISA) has emerged as a valuable method for detecting organic residues owing to its high sensitivity and specificity. Organic components have been observed within four categories of archaeological artifacts: mortars, adhesives, animal and plant remains, and daily use artifacts. Therefore, in this article, we critically analyzed the advantages and limitations of ELISA in detecting organic residues by tracking its recent application in the abovementioned categories. The current focus of ELISA applications is on the preparation of customized antibodies, development of multicomponent detection methods, and meeting on-site identification demands. Additionally, understanding organic residue degradation mechanisms and the proper handling of archaeological samples are also key factors in these applications. Integration of ELISA with biomolecular science and electrochemistry has allowed the development of comprehensive detection and analyses. In the future, ELISA will be capable of handling more complex and diverse analyses, revealing highly intricate information from archaeological samples.

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ELISA 在文化遗产中的应用:最新进展与挑战。
长期以来,有机残留物分析一直是科学考古和文化遗产领域的重点和难点。酶联免疫吸附法(ELISA)因其高灵敏度和特异性,已成为检测有机残留物的重要方法。在灰泥、粘合剂、动植物遗骸和日用器物这四类考古器物中都观察到了有机成分。因此,在本文中,我们通过跟踪 ELISA 在上述类别中的最新应用,批判性地分析了 ELISA 在检测有机残留物方面的优势和局限性。目前,ELISA 的应用重点在于制备定制抗体、开发多组分检测方法以及满足现场鉴定需求。此外,了解有机残留物的降解机制和正确处理考古样本也是这些应用的关键因素。将 ELISA 与生物分子科学和电化学相结合,可以开发出全面的检测和分析方法。未来,酶联免疫吸附法将能够处理更复杂、更多样的分析,揭示考古样本中错综复杂的信息。
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来源期刊
CiteScore
12.00
自引率
4.00%
发文量
137
审稿时长
6 months
期刊介绍: Critical Reviews in Analytical Chemistry continues to be a dependable resource for both the expert and the student by providing in-depth, scholarly, insightful reviews of important topics within the discipline of analytical chemistry and related measurement sciences. The journal exclusively publishes review articles that illuminate the underlying science, that evaluate the field''s status by putting recent developments into proper perspective and context, and that speculate on possible future developments. A limited number of articles are of a "tutorial" format written by experts for scientists seeking introduction or clarification in a new area. This journal serves as a forum for linking various underlying components in broad and interdisciplinary means, while maintaining balance between applied and fundamental research. Topics we are interested in receiving reviews on are the following: · chemical analysis; · instrumentation; · chemometrics; · analytical biochemistry; · medicinal analysis; · forensics; · environmental sciences; · applied physics; · and material science.
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