Green-synthesized copper oxide nanoparticles induce apoptosis and up-regulate HOTAIR and HOTTIP in pancreatic cancer cells.

IF 3.9 Nanomedicine (London, England) Pub Date : 2024-01-01 Epub Date: 2024-07-16 DOI:10.1080/17435889.2024.2367958

Zahra Hosseini, Amirhossein Ahmadi, Ahmad Shadi, Seyed Javad Hosseini, Hossein Nikmanesh

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Abstract

Aim: Cu₂O nanoparticles were synthesized using an extract from S. latifolium algae (SLCu₂O NPs). Their effect on PANC-1 cells and the expression of two drug resistance-related lncRNAs were evaluated in comparison with Arsenic trioxide.Materials & methods: SLCu₂O NPs were characterized using XRD, SEM, and TEM microscopies. The effects of SLCu₂O NPs on cell cytotoxicity, cell cycle, and apoptosis, and expression of two drug resistance-related lncRNAs were examined using MTT assay, flow cytometry, and real-time PCR, respectively.Results: SLCu₂O NPs demonstrated anti-cancer properties against PANC-1 cells comparable to Arsenic trioxide, and the expression of lncRNAs increased upon treatment with them.Conclusion: SLCu₂O NPs demonstrate anti-cancer properties against PANC-1 cells; however, using gene silencing strategies along with SLCu₂O NPs is suggested.

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绿色合成的氧化铜纳米粒子可诱导胰腺癌细胞凋亡并上调 HOTAIR 和 HOTTIP。

目的：利用拉蒂夫藻的提取物合成了Cu2O纳米粒子（SLCu2O NPs）。与三氧化二砷相比，评估了它们对 PANC-1 细胞的影响以及两种耐药性相关 lncRNA 的表达。材料与方法：使用 XRD、SEM 和 TEM 显微镜对 SLCu2O NPs 进行表征。采用 MTT 法、流式细胞术和实时 PCR 法分别检测了 SLCu2O NPs 对细胞毒性、细胞周期和细胞凋亡的影响，以及两种耐药性相关 lncRNAs 的表达。结果SLCu2O NPs对PANC-1细胞的抗癌作用与三氧化二砷相当，而且用其处理后lncRNAs的表达增加。结论SLCu2O NPs 对 PANC-1 细胞具有抗癌作用，但建议在使用 SLCu2O NPs 的同时使用基因沉默策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Nanomedicine (London, England)

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