Multi-walled carbon nanotubes-metal–organic framework nanocomposite based sensor for the monitoring of multiple monoamine neurotransmitters in living cells

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Bioelectrochemistry Pub Date : 2024-07-06 DOI:10.1016/j.bioelechem.2024.108776

Mengjie Su , Wenjing Peng , Zhengyuan Ding, Yaqiu Zhou, Hui Gao, Qiyu Jiang, Chunmei Yu

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Abstract

The levels of monoamine neurotransmitters (MNTs) including dopamine (DA), adrenaline (Adr), norepinephrine (NE) and 5-hydroxytryptamine (5-HT) in cells are useful indicators to explore the pathogenesis of MNTs-related diseases such as Alzheimer’s disease, Parkinson’s disease and depression. Herein, we constructed a novel electrochemical sensing platform based on multi-walled carbon nanotubes (MWCNTs)-amine functionalized Zr (IV) metal–organic framework (UIO-66-NH₂) nanocomposite for the detection of multiple MNTs including DA, Adr, NE and 5-HT. The synergistic effect between MWCNTs and UIO-66-NH₂ endowed the nanocomposite with high specific surface area, low interface impedance and superior electrocatalytic activity, which effectively enhance the electrochemical performance of the sensor. The MWCNTs-UIO-66-NH₂ nanocomposite-based sensor exhibited satisfied sensitivity for the quantitative measurement of DA, Adr, NE and 5-HT, as well as low detection limit. The outstanding biocompatibility of the constructed sensor permitted it to be successfully implemented for the real-time monitoring of DA released by PC12 and C6 cells, providing a promising strategy for clinical diagnosis of MNTs-related disorders and diseases.

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基于多壁碳纳米管-金属有机框架纳米复合材料的传感器，用于监测活细胞中的多种单胺类神经递质

细胞中多巴胺（DA）、肾上腺素（Adr）、去甲肾上腺素（NE）和 5-羟色胺（5-HT）等单胺神经递质（MNTs）的水平是探索阿尔茨海默病、帕金森病和抑郁症等 MNTs 相关疾病发病机制的有用指标。在此，我们构建了一种基于多壁碳纳米管（MWCNTs）-胺功能化 Zr (IV) 金属有机框架（UIO-66-NH2）纳米复合材料的新型电化学传感平台，用于检测包括 DA、Adr、NE 和 5-HT 在内的多种 MNTs。MWCNTs 和 UIO-66-NH2 的协同作用使纳米复合材料具有高比表面积、低界面阻抗和优异的电催化活性，从而有效提高了传感器的电化学性能。基于 MWCNTs-UIO-66-NH2 纳米复合材料的传感器对 DA、Adr、NE 和 5-HT 的定量测量具有良好的灵敏度和较低的检出限。所构建的传感器具有出色的生物相容性，可成功用于实时监测 PC12 和 C6 细胞释放的 DA，为 MNT 相关疾病的临床诊断提供了一种前景广阔的策略。

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来源期刊

Bioelectrochemistry 生物-电化学

CiteScore

9.10

自引率

6.00%

发文量

238

审稿时长

38 days

期刊介绍： An International Journal Devoted to Electrochemical Aspects of Biology and Biological Aspects of Electrochemistry Bioelectrochemistry is an international journal devoted to electrochemical principles in biology and biological aspects of electrochemistry. It publishes experimental and theoretical papers dealing with the electrochemical aspects of: • Electrified interfaces (electric double layers, adsorption, electron transfer, protein electrochemistry, basic principles of biosensors, biosensor interfaces and bio-nanosensor design and construction. • Electric and magnetic field effects (field-dependent processes, field interactions with molecules, intramolecular field effects, sensory systems for electric and magnetic fields, molecular and cellular mechanisms) • Bioenergetics and signal transduction (energy conversion, photosynthetic and visual membranes) • Biomembranes and model membranes (thermodynamics and mechanics, membrane transport, electroporation, fusion and insertion) • Electrochemical applications in medicine and biotechnology (drug delivery and gene transfer to cells and tissues, iontophoresis, skin electroporation, injury and repair). • Organization and use of arrays in-vitro and in-vivo, including as part of feedback control. • Electrochemical interrogation of biofilms as generated by microorganisms and tissue reaction associated with medical implants.