A viral effector blocks the turnover of a plant NLR receptor to trigger a robust immune response.

IF 9.4 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY EMBO Journal Pub Date : 2024-09-01 Epub Date: 2024-07-17 DOI:10.1038/s44318-024-00174-6
Chunli Wang, Min Zhu, Hao Hong, Jia Li, Chongkun Zuo, Yu Zhang, Yajie Shi, Suyu Liu, Haohua Yu, Yuling Yan, Jing Chen, Lingna Shangguan, Aiping Zhi, Rongzhen Chen, Karen Thulasi Devendrakumar, Xiaorong Tao
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Abstract

Plant intracellular nucleotide-binding and leucine-rich repeat immune receptors (NLRs) play a key role in activating a strong pathogen defense response. Plant NLR proteins are tightly regulated and accumulate at very low levels in the absence of pathogen effectors. However, little is known about how this low level of NLR proteins is able to induce robust immune responses upon recognition of pathogen effectors. Here, we report that, in the absence of effector, the inactive form of the tomato NLR Sw-5b is targeted for ubiquitination by the E3 ligase SBP1. Interaction of SBP1 with Sw-5b via only its N-terminal domain leads to slow turnover. In contrast, in its auto-active state, Sw-5b is rapidly turned over as SBP1 is upregulated and interacts with both its N-terminal and NB-LRR domains. During infection with the tomato spotted wilt virus, the viral effector NSm interacts with Sw-5b and disrupts the interaction of Sw-5b with SBP1, thereby stabilizing the active Sw-5b and allowing it to induce a robust immune response.

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一种病毒效应物阻断了植物 NLR 受体的转换,从而引发强大的免疫反应。
植物细胞内核苷酸结合和富亮氨酸重复免疫受体(NLRs)在激活强烈的病原体防御反应中发挥着关键作用。植物 NLR 蛋白受到严格调控,在没有病原体效应物的情况下以极低水平积累。然而,人们对这种低水平的 NLR 蛋白如何在识别病原体效应物时诱导强有力的免疫反应知之甚少。在这里,我们报告了在没有效应物的情况下,番茄 NLR Sw-5b 的非活性形式被 E3 连接酶 SBP1 靶向泛素化。SBP1 仅通过其 N 端结构域与 Sw-5b 相互作用,导致其周转缓慢。与此相反,在自动激活状态下,Sw-5b 会随着 SBP1 的上调并与其 N 端和 NB-LRR 结构域相互作用而迅速转换。在番茄斑萎病毒感染过程中,病毒效应物 NSm 与 Sw-5b 相互作用,破坏 Sw-5b 与 SBP1 的相互作用,从而稳定活性 Sw-5b,使其能够诱导强有力的免疫反应。
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来源期刊
EMBO Journal
EMBO Journal 生物-生化与分子生物学
CiteScore
18.90
自引率
0.90%
发文量
246
审稿时长
1.5 months
期刊介绍: The EMBO Journal has stood as EMBO's flagship publication since its inception in 1982. Renowned for its international reputation in quality and originality, the journal spans all facets of molecular biology. It serves as a platform for papers elucidating original research of broad general interest in molecular and cell biology, with a distinct focus on molecular mechanisms and physiological relevance. With a commitment to promoting articles reporting novel findings of broad biological significance, The EMBO Journal stands as a key contributor to advancing the field of molecular biology.
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