Exploring membrane proteins dynamic in plant cells with fluorescence correlation spectroscopy

New Crops Pub Date : 2024-07-01 DOI:10.1016/j.ncrops.2024.100032

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Abstract

Biomolecule interactions and macromolecular rearrangement participate in numerous cellular functions in plants, and resolving the dynamics of plasma membrane proteins represents a central goal in current plant biology. Compared to yeast and mammalian systems, the quantification of heterogeneous distribution and dynamics of membrane proteins in cellular processes remains sparse in plant cells. In this study, we introduce the application of ﬂuorescence correlation spectroscopy (FCS) and ﬂuorescence cross-correlation spectroscopy (FCCS) in measuring membrane protein diffusion, concentration and interactions in living plant cell. The review showed FCS/FCCS as a tool for imaging the membrane proteins fused with a fluorescent tag, quantifying the density fluctuation and interactions of membrane proteins in the living cells of plants. Owing to the single-molecular level sensitivity and minimally invasive of FCS/FCCS, their application provides an ideal approach to understanding plant cell membrane lateral organization.

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利用荧光相关光谱探索植物细胞中的膜蛋白动态

生物分子相互作用和大分子重排参与了植物细胞的许多功能，而解析质膜蛋白的动态是当前植物生物学的一个核心目标。与酵母和哺乳动物系统相比，植物细胞中膜蛋白在细胞过程中的异质分布和动态的定量研究仍然很少。本研究介绍了荧光相关光谱（FCS）和荧光交叉相关光谱（FCCS）在测量活体植物细胞中膜蛋白扩散、浓度和相互作用方面的应用。综述显示，FCS/FCCS 是对融合了荧光标签的膜蛋白进行成像的工具，可量化植物活细胞中膜蛋白的密度波动和相互作用。由于 FCS/FCCS 具有单分子水平的灵敏度和微创性，其应用为了解植物细胞膜横向组织提供了一种理想的方法。

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New Crops

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