Role of urinary tract microbiota and urine proteome in urolithiasis pathogenesis

Abstract

The pathogenetic approach to the metaphylaxis of urolithiasis is universally a socially significant health problem due to the increase in morbidity and, especially, its recurrent course, despite the achievements of pharmacotherapy and the use of lithotripsy. Pathological crystallogenesis is considered as a consequence of exceeding the solubility threshold of a number of mineral components of urine, on the one hand, and as a consequence of posttranslational defects of the main proteome of urination - uromodulin (UMOD), demonstrating in healthy people a sanogenetic system for ensuring the stability of biophysical homeostasis: colloidal properties of urine. However, in addition, UMOD, by binding mannose-dependent pills of infectious agents, blocks the contact of bacteria with urotheliocytes, despite the fact that infection of the urinary tract is a known factor of urolithiasis. By modeling the urine of healthy individuals by coincubation with E. coli bacteria, a decrease in the concentration of polymerized uromodulin in urine was demonstrated as a factor in increasing the risk of formation of calcium oxalate crystals