Isolated and Associated Use of the Nematophagous Fungi Pochonia chlamydosporia and Duddingtonia flagrans to Control Taenia saginata Eggs

Thaís Schmidt Ferreira, Carolina Magri Ferraz, Pedro Henrique Dutra Santos, F. E. Soares, Vinicius Bastos Salles Segantine, V. Vilela, J. V. Araújo, F. R. Braga
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Abstract

The aim of this study was to evaluate the isolated and associated use of the nematophagous fungi Pochonia chlamydosporia (isolate VC4) as an ovicide and Duddingtonia flagrans (isolate AC001) as a predator in the control of Taenia saginata eggs. Viable T. saginata eggs were obtained by dissecting mature proglottids from a specimen. For the experimental assay, four groups were formed in microtubes, as follows: (G1—T. saginata eggs/control); (G2—100 T. saginata eggs + VC4); (G3—100 T. saginata eggs + AC001); (G4—100 T. saginata eggs + VC4 + AC001). All the groups were stored in a B.O.D. incubation chamber at a temperature of 27 °C for 15 days and then the contents of the microtubes were analyzed using an optical microscope with a 40x objective. At the end of the assay the treated groups (G2 to G4) showed ovicidal activity (destruction of eggs) compared to the control group (G1). The highest ovicidal percentage was observed in group G2 (eggs + VC4), with 43.3%. In groups G3 and G4 (combination of fungal isolates), the ovicidal percentages were 25.7% and 25.6%, respectively. The results of this study shed light on a new possibility for the combined use of different species of nematophagous fungi, which could be used in the future for environmental biological control of T. saginata eggs.
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利用分离出来的食线虫真菌 Pochonia chlamydosporia 和 Duddingtonia flagrans 控制矢车菊球蛆卵及其相关用途
本研究的目的是评估分离出的线虫真菌 Pochonia chlamydosporia(分离物 VC4)作为杀卵剂和 Duddingtonia flagrans(分离物 AC001)作为捕食者在控制矢车菊弓形虫卵方面的相关用途。从标本中剖开成熟的前鞭毛虫,即可获得有活力的矢车菊球虫卵。实验中,在微管中分成以下四组:(G1-矢车菊卵/对照组);(G2-100 个矢车菊卵 + VC4);(G3-100 个矢车菊卵 + AC001);(G4-100 个矢车菊卵 + VC4 + AC001)。所有组别均在温度为 27 °C 的 B.O.D. 培养室中保存 15 天,然后使用带 40 倍物镜的光学显微镜分析微管中的内容物。试验结束时,与对照组(G1)相比,处理组(G2 至 G4)显示出了杀卵活性(卵被破坏)。G2 组(虫卵 + VC4)的杀卵率最高,为 43.3%。G3 和 G4 组(真菌分离物组合)的杀卵率分别为 25.7% 和 25.6%。该研究结果为联合使用不同种类的食线虫真菌提供了一种新的可能性,未来可将其用于矢车菊虫卵的环境生物防治。
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