STUDY OF THE PHYSIOLOGICAL ROLE OF STREBLUS ASPER AS A CHEMOPREVENTIVE AGENT ON HUMAN PROSTATE CANCER (DU-145) CELL LINE

Abstract

Objectives: This study aims to explore the chemopreventive potential of Streblus asper extracts against prostate cancer cells. Specifically, it seeks to assess the efficacy of these extracts in inducing apoptosis and inhibiting cell proliferation within these cancer cells. Methods: The inhibitory effects of S. asper extracts were evaluated using the TBEA technique to determine the IC50 values. Morphological changes were indicative of apoptosis, such as cell shrinkage, rounding, vacuolization, formation of apoptotic bodies, membrane blebbing, and cell elongation were observed following treatment. An 8-day cell proliferation assay was conducted to examine the effects on prostate cancer cell line proliferation. In addition, the safety of S. asper root extracts was assessed in vitro using a Mouse fibroblast cell line to ensure no cytotoxic effects were present on normal cells. Results: Streblus asper extracts demonstrated a significant inhibitory effect on the proliferation of prostate cancer cells, with no observed cytotoxicity on normal Mouse fibroblast cell lines. The results from the TBEA technique confirmed the extract’s potency, with noticeable morphological changes supporting the induction of apoptosis in the treated cancer cells. Conclusion: The findings from this study offer promising insights into the potential of S. asper extracts as chemopreventive agents against prostate cancer. By exhibiting significant inhibitory effects on cancer cell proliferation and inducing apoptosis without harming normal cells, S. asper presents a viable, natural alternative in the fight against prostate cancer. Further research and clinical trials are warranted to fully elucidate its therapeutic potential and application in cancer prevention strategies.