Amino acid mutation of succinate dehydrogenase complex induced resistance to benzovindiflupyr in Magnaporthe oryzae

IF 4.2 1区农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pesticide Biochemistry and Physiology Pub Date : 2024-07-15 DOI:10.1016/j.pestbp.2024.106027

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Abstract

Magnaporthe oryzae is a rice blast pathogen that seriously threatens rice yield. Benzovindiflupyr is a succinate dehydrogenase inhibitor (SDHI) fungicide that effectively controls many crop diseases. Benzovindiflupyr has a strong inhibitory effect on M. oryzae; however, control of rice blast by benzovindiflupyr and risk of resistance to benzovindiflupyr are not well studied in this pathogen. In this study, six benzovindiflupyr-resistant strains were obtained by domestication induced in the laboratory. The MoSdhB^H245D mutation was the cause of M. oryzae resistance to benzovindiflupyr, which was verified through succinate dehydrogenase (SDH) activity assays, molecular docking, and site-specific mutations. Survival fitness analysis showed no significant difference between the benzovindiflupyr-resistant and parent strains. Positive cross-resistance to benzovindiflupyr and other SDHIs and negative cross-resistance to azoxystrobin were observed. Therefore, the risk of benzovindiflupyr resistance in M. oryzae might be medium to high. It should be combined with other classes of fungicides (tebuconazole and azoxystrobin) to slow the development of resistance.

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琥珀酸脱氢酶复合物的氨基酸突变诱导木兰根腐菌对苯并吲哚氟吡啶产生抗性

Magnaporthe oryzae 是一种严重威胁水稻产量的稻瘟病病原体。苯并茚虫威是一种琥珀酸脱氢酶抑制剂（SDHI）杀菌剂，可有效控制多种作物病害。苯并茚虫威对稻瘟病菌（M. oryzae）有很强的抑制作用，但苯并茚虫威对稻瘟病菌的控制作用以及稻瘟病菌对苯并茚虫威产生抗性的风险还没有得到很好的研究。在本研究中，通过实验室驯化诱导获得了六株抗苯并茚虫威的菌株。通过琥珀酸脱氢酶（SDH）活性测定、分子对接和位点特异性突变验证，MoSdhBH245D突变是导致M. oryzae对苯并茚虫威产生抗性的原因。生存适应性分析表明，苯并茚虫威抗性菌株与亲本菌株之间没有显著差异。对苯并嘧啶氟吡啶和其他 SDHIs 的交叉抗性为阳性，对唑氧菌酯的交叉抗性为阴性。因此，M. oryzae 对苯并茚虫威产生抗性的风险可能为中高。应将苯醚甲环唑与其他类杀菌剂（戊唑醇和唑醚菌酯）结合使用，以减缓抗药性的发展。

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来源期刊

Pesticide Biochemistry and Physiology 生物-昆虫学

CiteScore

7.00

自引率

8.50%

发文量

238

审稿时长

4.2 months

期刊介绍： Pesticide Biochemistry and Physiology publishes original scientific articles pertaining to the mode of action of plant protection agents such as insecticides, fungicides, herbicides, and similar compounds, including nonlethal pest control agents, biosynthesis of pheromones, hormones, and plant resistance agents. Manuscripts may include a biochemical, physiological, or molecular study for an understanding of comparative toxicology or selective toxicity of both target and nontarget organisms. Particular interest will be given to studies on the molecular biology of pest control, toxicology, and pesticide resistance. Research Areas Emphasized Include the Biochemistry and Physiology of: • Comparative toxicity • Mode of action • Pathophysiology • Plant growth regulators • Resistance • Other effects of pesticides on both parasites and hosts.