Biocontrol of Fusarium spp. in vitro and in vine cuttings using Bacillus sp. F62

Q3 Agricultural and Biological Sciences Advances in horticultural science Pub Date : 2024-07-01 DOI:10.36253/ahsc-15354

Hélen Corso Cavião, Alessandra Russi, J. Schwambach

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Abstract

Fusarium spp., the causal agent of Fusarium wilt, cause substantial economic losses in viticulture, mainly in tropical regions. This study aimed to assess the biocontrol potential of Bacillus sp. F62 against Fusarium spp., both in vitro and in rootstock cuttings of the SO4 variety. To this end, the in vitro antagonism was evaluated through diffusible and volatile compounds synthesized by Bacillus sp. F62 on three Fusarium spp. isolates. Subsequently, the isolate FusA06-18 was selected for a rootstock cutting experiment. The vine cuttings underwent the following treatments: control, pathogen inoculation (Fus), bacterial inoculation (Bac), and bacterial followed by pathogen inoculation (Bac + Fus). Our findings revealed an average reduction of 39.1% in the mycelial growth of the pathogen through dual culture assay and a decrease of 11.6% in the Fusarium spp. radial growth due to the effects of volatile compounds. In the experiment with vine cuttings, applying Bacillus sp. F62 reduced the pathogen re-isolation frequency from 81.7% (Fus) to 63.3% (Bac + Fus). Therefore, Bacillus sp. F62 effectively suppressed the mycelial growth of Fusarium spp. and reduced the Fusarium wilt incidence in vine cuttings of the rootstock ‘SO4’.

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利用芽孢杆菌 F62 在体外和葡萄树插条中对镰刀菌属进行生物防治

镰刀菌属是镰刀菌枯萎病的病原菌，对葡萄栽培造成了巨大的经济损失，主要发生在热带地区。本研究旨在评估芽孢杆菌 F62 在体外和 SO4 品种根茎插条中对镰刀菌属的生物防治潜力。为此，通过芽孢杆菌 F62 对三种镰刀菌属分离物合成的扩散性和挥发性化合物，对其体外拮抗作用进行了评估。随后，分离物 FusA06-18 被选中用于根茎扦插实验。扦插的葡萄藤经过了以下处理：对照、病原体接种（Fus）、细菌接种（Bac）和细菌接种后病原体接种（Bac + Fus）。我们的研究结果表明，在挥发性化合物的作用下，通过双重培养试验，病原体的菌丝生长平均减少了 39.1%，镰刀菌的径向生长减少了 11.6%。在葡萄树扦插实验中，使用芽孢杆菌 F62 可将病原菌的再分离频率从 81.7%（镰刀菌）降至 63.3%（芽孢杆菌 + 镰刀菌）。因此，芽孢杆菌 F62 能有效抑制镰刀菌菌丝体的生长，降低砧木 "SO4 "的镰刀菌枯萎病发病率。

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来源期刊

Advances in horticultural science Agricultural and Biological Sciences-Horticulture

CiteScore

1.20

自引率

0.00%

发文量

审稿时长

12 weeks

期刊介绍： Advances in Horticultural Science aims to provide a forum for original investigations in horticulture, viticulture and oliviculture. The journal publishes fully refereed papers which cover applied and theoretical approaches to the most recent studies of all areas of horticulture - fruit growing, vegetable growing, viticulture, floriculture, medicinal plants, ornamental gardening, garden and landscape architecture, in temperate, subtropical and tropical regions. Papers on horticultural aspects of agronomic, breeding, biotechnology, entomology, irrigation and plant stress physiology, plant nutrition, plant protection, plant pathology, and pre and post harvest physiology, are also welcomed. The journal scope is the promotion of a sustainable increase of the quantity and quality of horticultural products and the transfer of the new knowledge in the field. Papers should report original research, should be methodologically sound and of relevance to the international scientific community. AHS publishes three types of manuscripts: Full-length - short note - review papers. Papers are published in English.