Advances in genomic toxicology: In vitro developmental toxicity test based on signal network disruption dynamics

Yusuke Okubo , Yoko Hirabayashi , Junji Fukuda
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Abstract

Developmental toxicity outcomes in humans and animals often exhibit variability; hence, the demand for predictive non-animal alternatives, particularly human cell-based models, are increasing. Despite advancements in genomic toxicology, which have facilitated the identification of toxicity mechanisms and potential biomarkers, existing transcriptome analysis-based methods have yet to yield highly predictive in vitro developmental toxicity assays. One possible reason is that assays at a single time point could not capture the entire dynamic signal network during developmental processes. This article addresses the challenges in comprehensive gene expression analysis and introduces novel in vitro developmental toxicity assays focused on the time-dependent dynamics of signaling pathway responses crucial to human development.

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基因组毒理学的进展:基于信号网络中断动力学的体外发育毒性测试
人类和动物的发育毒性结果往往具有变异性;因此,对具有预测性的非动物替代方法,特别是基于人类细胞的模型的需求日益增加。尽管基因组毒理学的进步促进了毒性机制和潜在生物标志物的鉴定,但现有的基于转录组分析的方法尚未产生具有高度预测性的体外发育毒性试验。其中一个可能的原因是单一时间点的检测无法捕捉发育过程中的整个动态信号网络。本文探讨了全面基因表达分析所面临的挑战,并介绍了新型体外发育毒性检测方法,重点关注对人类发育至关重要的信号通路反应的时间依赖性动态。
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来源期刊
Current opinion in toxicology
Current opinion in toxicology Toxicology, Biochemistry
CiteScore
8.50
自引率
0.00%
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0
审稿时长
64 days
期刊最新文献
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