Optimisation and Evaluation of the Correlation of Extraction Conditions for Total Flavonoid Content and Antixanthine Oxidase Activity in Psidium guajava

IF 2.2 4区 医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE Journal of Herbal Medicine Pub Date : 2024-07-14 DOI:10.1016/j.hermed.2024.100915
T.M. Le , C.D.P. Nguyen , A.C. Ha
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Abstract

Introduction

Psidium guajava L. (P guajava) and its leaf, one of the most popular plants in traditional medicine and cuisine, were demonstrated to have high flavonoid content with many bioactivities, including antigout activity. However, the correlation between total flavonoid content (TFC) and antigout activities and the optimal conditions for extracting P guajava have not been reported.

Methods

This study established the optimisation of ethanol extracts of P guajava leaves for TFC and antigout activities using the single-factor method and response surface methodology. The antigout activities were determined through the xanthine oxidase inhibition (XOI) method.

Results

The four independent variables (ethanol concentration, plant-solvent ratio, extraction time, and temperature) significantly impacted two response variables (TFC and XOI activity). The optimal extracts for XOI activity reach the maximum TFC of 323.86 ± 2.26 mg quercetin/g and XOI activity of 26.37 ± 0.5 μg/ml. The changes in the chemical structure of extracts were analysed by Fourier transform infrared, which indicated that several flavonoids in P guajava do not have XOI activity. The correlation of TFC and XOI is robust with the Pearson correlation of −0.882 and Spearman Rho test of −0.884. Additionally, the biological activities, such as antioxidant and anti-inflammatory, of optimal extracts for XOI and TFC were determined to extend the application capacity to support the treatment of gout diseases.

Conclusions

The optimised extraction conditions are critical to isolating flavonoids, which can serve as functional food or medicine for managing gout and its complications.

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优化和评估萃取条件对番石榴总黄酮含量和抗黄嘌呤氧化酶活性的相关性
引言 番石榴(Psidium guajava L.)及其叶片是传统医学和烹饪中最受欢迎的植物之一,其黄酮类化合物含量高,具有多种生物活性,包括抗痛风活性。本研究采用单因素法和响应面法对番石榴叶乙醇提取物的总黄酮含量和抗痛风活性进行了优化。结果四个自变量(乙醇浓度、植物-溶剂比率、提取时间和温度)对两个响应变量(TFC 和 XOI 活性)有显著影响。XOI 活性的最佳提取物达到了最大 TFC(323.86 ± 2.26 毫克槲皮素/克)和 XOI 活性(26.37 ± 0.5 微克/毫升)。通过傅立叶变换红外光谱分析了提取物化学结构的变化,结果表明番石榴中的几种黄酮类化合物不具有 XOI 活性。TFC 与 XOI 的相关性很强,Pearson 相关性为-0.882,Spearman Rho 检验为-0.884。此外,还确定了 XOI 和 TFC 最佳提取物的抗氧化和抗炎等生物活性,以扩大其应用能力,支持痛风疾病的治疗。
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来源期刊
Journal of Herbal Medicine
Journal of Herbal Medicine INTEGRATIVE & COMPLEMENTARY MEDICINE-
CiteScore
3.90
自引率
0.00%
发文量
94
期刊介绍: The Journal of Herbal Medicine, the official journal of the National Institute of Medical Herbalists, is a peer reviewed journal which aims to serve its readers as an authoritative resource on the profession and practice of herbal medicine. The content areas of the journal reflect the interests of Medical Herbalists and other health professionals interested in the clinical and professional application of botanical medicines. The objective is to strengthen the research and educational base of herbal medicine with research papers in the form of case studies, original research articles and reviews, monographs, clinical trials and relevant in vitro studies. It also publishes policy statements, opinion pieces, book reviews, conference proceedings and profession related information such as pharmacovigilance reports providing an information source for not only the Herbal Practitioner but any Health professional with an interest in phytotherapy.
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