Organic-inorganic nHA-Gelatin/Alginate high strength macroporous cryogel promotes bone regeneration

Q1 Engineering Smart Materials in Medicine Pub Date : 2024-07-16 DOI:10.1016/j.smaim.2024.07.001

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Abstract

Macroporous cryogel has the advantages of nutrient exchange and cell growth, and is an ideal material for tissue regeneration. In order to strengthen the machenical properties of cryogel for the widely use, a high strength gelatin/sodium alginate/nano hydroxyapatite (nHA) porous cryogel (GA-HA cryogel) was prepared by a simple freeze-thaw process. The mechanical strength of GA-HA cryogel increased significantly with the increase of nHA content. In vitro studies showed that GA-HA cryogel had good biocompatibility and no obvious cytotoxicity to MC3T3-E1 cells. The results of alkaline phosphatase activity assay and osteocalcin immunofluorescence staining showed that GA-HA1 porous hydrogel system could significantly increase the expression of MC3T3-E1 alkaline phosphatase and osteocalcin when the content of nHA was 1 %. In addition, porous GA-HA cryogel showed good performance in promoting bone regeneration in rat skull defect model. Therefore, the high-strength double network cryogel prepared in this study can provide new applications in bone repair and tissue regeneration.

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有机-无机 nHA-明胶/海藻酸高强度大孔低温凝胶促进骨再生

大孔低温凝胶具有营养交换和细胞生长的优点，是组织再生的理想材料。为了增强低温凝胶的宏观性能，使其得到广泛应用，研究人员采用简单的冻融工艺制备了一种高强度明胶/海藻酸钠/纳米羟基磷灰石（nHA）多孔低温凝胶（GA-HA cryogel）。随着 nHA 含量的增加，GA-HA 低温凝胶的机械强度显著提高。体外研究表明，GA-HA 低温凝胶具有良好的生物相容性，对 MC3T3-E1 细胞无明显的细胞毒性。碱性磷酸酶活性测定和骨钙素免疫荧光染色结果表明，当nHA含量为1%时，GA-HA1多孔水凝胶系统能显著提高MC3T3-E1碱性磷酸酶和骨钙素的表达。此外，多孔 GA-HA 低温凝胶在促进大鼠颅骨缺损模型的骨再生方面表现良好。因此，本研究制备的高强度双网络低温凝胶可在骨修复和组织再生方面提供新的应用。

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