What is the impact of Endodontic Irrigant Solutions on the Viability of Stem Cells from Apical Papilla in an in-vitro setting: A Systematic Review

IF 1.7 Q3 DENTISTRY, ORAL SURGERY & MEDICINE Saudi Dental Journal Pub Date : 2024-07-14 DOI:10.1016/j.sdentj.2024.07.006

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Abstract

Background

The success of regenerative endodontic procedures (REPs) is significantly influenced by the choice of endodontic irrigant solution. However, the impact of these solutions on the viability of stem cells from the apical papilla (SCAP), a critical component of the REP, remains a subject of ongoing debate.

Objective

This study aimed to investigate the effects of various endodontic irrigant solutions on the viability of stem cells from the apical papilla in an in vitro setting.

Methods

A systematic literature search was conducted using databases such as PubMed/Medline, Scopus, the Cochrane Library, Web of Science, Embase, gray literature, and reference lists up to August 2023. The search was limited to in vitro studies investigating the impact of endodontic irrigant solutions on SCAP viability. The risk of bias in these studies was evaluated using the Joanna Briggs Institute’s checklist.

Results

Of the 131 articles retrieved, 14 were selected for review. The effects of eighteen different root canal irrigants, such as ethylenediaminetetraacetic acid, sodium hypochlorite, chlorhexidine, and citric acid, on the viability of SCAPs were evaluated. The risk-of-bias analysis showed a high risk in sample randomization and size justification but a low risk in other areas.

Discussion

The effects of endodontic irrigant solutions on the viability of SCAPs are concentration dependent. Concentrations higher than 1.5% sodium hypochlorite, 2 % chlorhexidine, 10 % citric acid, and 2.5 % EDTA significantly reduced cell viability. However, additional research is necessary to determine the effect of these irrigants on tissue regeneration.

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体外评估牙髓冲洗液对根尖乳头干细胞活力的影响：系统性综述

背景牙髓再生术（REP）的成功与否在很大程度上受到牙髓冲洗液选择的影响。然而，这些溶液对牙髓根尖乳头（SCAP）干细胞存活率的影响（SCAP是REP的关键组成部分）仍是一个持续争论的话题。本研究旨在体外环境中调查各种牙髓冲洗液对牙髓根尖乳头干细胞存活率的影响。方法使用PubMed/Medline、Scopus、Cochrane图书馆、Web of Science、Embase、灰色文献和截至2023年8月的参考文献列表等数据库进行了系统的文献检索。搜索仅限于调查根管治疗冲洗液对 SCAP 活力影响的体外研究。结果在检索到的 131 篇文章中，有 14 篇被选中进行综述。对乙二胺四乙酸、次氯酸钠、洗必泰、柠檬酸等 18 种不同根管冲洗液对 SCAP 存活率的影响进行了评估。偏倚风险分析表明，样本随机化和规模合理性方面的风险较高，但其他方面的风险较低。讨论牙髓冲洗液对 SCAPs 存活率的影响与浓度有关。浓度高于 1.5% 次氯酸钠、2% 洗必泰、10% 柠檬酸和 2.5% EDTA 会显著降低细胞活力。不过，要确定这些冲洗剂对组织再生的影响，还需要进行更多的研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Saudi Dental Journal DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

3.60

自引率

0.00%

发文量

审稿时长

22 weeks

期刊介绍： Saudi Dental Journal is an English language, peer-reviewed scholarly publication in the area of dentistry. Saudi Dental Journal publishes original research and reviews on, but not limited to: • dental disease • clinical trials • dental equipment • new and experimental techniques • epidemiology and oral health • restorative dentistry • periodontology • endodontology • prosthodontics • paediatric dentistry • orthodontics and dental education Saudi Dental Journal is the official publication of the Saudi Dental Society and is published by King Saud University in collaboration with Elsevier and is edited by an international group of eminent researchers.