Frontiers | Development and antioxidant evaluation of mango leaf (Mangifera indica L.) extract loaded silk fibroin nanoparticles

IF 2.6 4区 材料科学 Q3 MATERIALS SCIENCE, MULTIDISCIPLINARY Frontiers in Materials Pub Date : 2024-06-12 DOI:10.3389/fmats.2024.1419697
Nguyen Thi Ngoc Phuong, My Tien Ha, Doan Xuan Tien Nguyen, Ngoc Yen Nguyen, Huynh Anh Thi Huynh, Trieu Phu Hau, Tran Thi Bich Quyen, Manh Quan Nguyen, Anh Tuan Nguyen, Duy Toan Pham
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Abstract

The main antioxidant polyphenol compounds in the mango (Mangifera indica L.) leaf extract are susceptible to environmental degradations. Thus, in biomedical applications, the mango leaf extract is commonly encapsulated in a carrier. However, most studies employed the synthetic carrier materials that could affect the human health, and the complicated formulation procedure that could hinder the scalability. Therefore, this work, for the first time, explored the use of silk fibroin (an FDA-approved biomaterial), in nanoparticles platform, to encapsulate and deliver the mango leaf extract, utilizing the simple coacervation preparation method. Initially, the mango leaf ethanolic extract was obtained through maceration, resulting in a total phenolic content of 76.39 ± 0.14 mg GAE/g DPW and a notably high antioxidant activity (IC50 = 6.872 ± 0.512 μg/mL). Subsequently, silk fibroin nanoparticles loaded with the extract were developed by the coacervation technique. Depending on the fibroin content, these nanoparticles exhibited an appropriate size range of 500–800 nm with narrow size distributions, a spherical shape with smooth surfaces, a dominant silk-II crystalline structure, a drug entrapment efficiency exceeding 70%, and retained the main biomarker mangiferin. Moreover, the phenolic-compounds release profiles from the particles followed the three-step process, the first burst-release step, the second sustained-release step, and the third degradation step. The particles were also non-toxic to the erythrocytes and the human embryonic kidney HEK-293 cell line. Lastly, the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay demonstrated that the antioxidant activity of the mango leaf extract was preserved within the extract-loaded nanoparticles. The results suggested that the silk fibroin nanoparticles could be a potential platform to effectively encapsulate and deliver the mango leaf extract for biomedical purposes.
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前沿|芒果叶(Mangifera indica L.)提取物负载丝纤维蛋白纳米颗粒的开发与抗氧化评估
芒果(Mangifera indica L.)叶提取物中的主要抗氧化多酚化合物易受环境降解的影响。因此,在生物医学应用中,芒果叶提取物通常被封装在载体中。然而,大多数研究采用的合成载体材料可能会影响人体健康,而且复杂的配制过程可能会阻碍其可扩展性。因此,本研究首次探索在纳米颗粒平台中使用蚕丝纤维素(一种经美国 FDA 批准的生物材料),利用简单的共凝制备方法来封装和递送芒果叶提取物。最初,芒果叶乙醇提取物是通过浸渍获得的,总酚含量为 76.39 ± 0.14 mg GAE/g DPW,抗氧化活性明显较高(IC50 = 6.872 ± 0.512 μg/mL)。随后,通过共凝固技术,开发出了负载该提取物的蚕丝纤维素纳米粒子。根据纤维素含量的不同,这些纳米颗粒的粒度范围为 500-800 nm,粒度分布窄,呈球形,表面光滑,主要为丝-II 结晶结构,药物包载效率超过 70%,并保留了主要的生物标志物芒果苷。此外,颗粒中酚类化合物的释放过程分为三步:第一步爆发释放,第二步持续释放,第三步降解。颗粒对红细胞和人类胚胎肾脏 HEK-293 细胞系也无毒性。最后,2,2-二苯基-1-苦基肼(DPPH)检测表明,芒果叶提取物的抗氧化活性在提取物负载的纳米颗粒中得以保留。研究结果表明,丝纤维蛋白纳米颗粒可以作为一个潜在的平台,有效地封装和输送芒果叶提取物,用于生物医学目的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Frontiers in Materials
Frontiers in Materials Materials Science-Materials Science (miscellaneous)
CiteScore
4.80
自引率
6.20%
发文量
749
审稿时长
12 weeks
期刊介绍: Frontiers in Materials is a high visibility journal publishing rigorously peer-reviewed research across the entire breadth of materials science and engineering. This interdisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers across academia and industry, and the public worldwide. Founded upon a research community driven approach, this Journal provides a balanced and comprehensive offering of Specialty Sections, each of which has a dedicated Editorial Board of leading experts in the respective field.
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