Ezrin, radixin, and moesin are dispensable for macrophage migration and cellular cortex mechanics.

IF 9.4 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY EMBO Journal Pub Date : 2024-11-01 Epub Date: 2024-07-18 DOI:10.1038/s44318-024-00173-7
Perrine Verdys, Javier Rey Barroso, Adeline Girel, Joseph Vermeil, Martin Bergert, Thibaut Sanchez, Arnaud Métais, Thomas Mangeat, Elisabeth Bellard, Claire Bigot, Catherine Astarie-Dequeker, Arnaud Labrousse, Jean-Philippe Girard, Isabelle Maridonneau-Parini, Christel Vérollet, Frédéric Lagarrigue, Alba Diz-Muñoz, Julien Heuvingh, Matthieu Piel, Olivia du Roure, Véronique Le Cabec, Sébastien Carréno, Renaud Poincloux
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引用次数: 0

Abstract

The cellular cortex provides crucial mechanical support and plays critical roles during cell division and migration. The proteins of the ERM family, comprised of ezrin, radixin, and moesin, are central to these processes by linking the plasma membrane to the actin cytoskeleton. To investigate the contributions of the ERM proteins to leukocyte migration, we generated single and triple ERM knockout macrophages. Surprisingly, we found that even in the absence of ERM proteins, macrophages still form the different actin structures promoting cell migration, such as filopodia, lamellipodia, podosomes, and ruffles. Furthermore, we discovered that, unlike every other cell type previously investigated, the single or triple knockout of ERM proteins does not affect macrophage migration in diverse contexts. Finally, we demonstrated that the loss of ERMs in macrophages does not affect the mechanical properties of their cortex. These findings challenge the notion that ERMs are universally essential for cortex mechanics and cell migration and support the notion that the macrophage cortex may have diverged from that of other cells to allow for their uniquely adaptive cortical plasticity.

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Ezrin、radixin 和 moesin 对于巨噬细胞迁移和细胞皮层力学是不可或缺的。
细胞皮层提供重要的机械支持,并在细胞分裂和迁移过程中发挥关键作用。由 ezrin、radixin 和 moesin 组成的 ERM 家族蛋白通过连接质膜和肌动蛋白细胞骨架,在这些过程中发挥着核心作用。为了研究ERM蛋白对白细胞迁移的贡献,我们产生了单个和三个ERM基因敲除的巨噬细胞。令人惊讶的是,我们发现即使在缺乏ERM蛋白的情况下,巨噬细胞仍能形成促进细胞迁移的不同肌动蛋白结构,如丝状体、片状体、荚膜和褶皱。此外,我们还发现,与之前研究过的其他细胞类型不同,单次或三次敲除ERM蛋白不会影响巨噬细胞在不同环境下的迁移。最后,我们证明巨噬细胞中 ERM 的缺失不会影响其皮质的机械特性。这些发现质疑了ERMs对皮质力学和细胞迁移普遍至关重要的观点,并支持了巨噬细胞皮质可能与其他细胞的皮质不同,从而使其具有独特的适应性皮质可塑性的观点。
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来源期刊
EMBO Journal
EMBO Journal 生物-生化与分子生物学
CiteScore
18.90
自引率
0.90%
发文量
246
审稿时长
1.5 months
期刊介绍: The EMBO Journal has stood as EMBO's flagship publication since its inception in 1982. Renowned for its international reputation in quality and originality, the journal spans all facets of molecular biology. It serves as a platform for papers elucidating original research of broad general interest in molecular and cell biology, with a distinct focus on molecular mechanisms and physiological relevance. With a commitment to promoting articles reporting novel findings of broad biological significance, The EMBO Journal stands as a key contributor to advancing the field of molecular biology.
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