Validation of a rapid collagenase activity detection technique based on fluorescent quenched gelatin with synovial fluid samples.

IF 3.5 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY BMC Biotechnology Pub Date : 2024-07-19 DOI:10.1186/s12896-024-00869-y
Miguel de la Fuente, Diego Delgado, Maider Beitia, Gabriel Barreda-Gómez, Arantxa Acera, Mikel Sanchez, Elena Vecino
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Abstract

Background: Measuring collagenase activity is crucial in the field of joint health and disease management. Collagenases, enzymes responsible for collagen degradation, play a vital role in maintaining the balance between collagen synthesis and breakdown in joints. Dysregulation of collagenase activity leads to joint tissue degradation and diseases such as rheumatoid arthritis and osteoarthritis. The development of methods to measure collagenase activity is essential for diagnosis, disease severity assessment, treatment monitoring, and identification of therapeutic targets.

Results: This study aimed to validate a rapid collagenase activity detection technique using synovial fluid samples. Antibody microarray analysis was initially performed to quantify the levels of matrix metalloproteinase-9 (MMP-9), a major collagenase in joints. Subsequently, the developed gelatin-based test utilizing fluorescence measurement was used to determine collagenase activity. There was a significant correlation between the presence of MMP-9 and collagenase activity. In addition, Lower Limit of Detection and Upper Limit of Detection can be preliminary estimated as 8 ng/mL and 48 ng/mL respectively.

Conclusions: The developed technique offers a potential point-of-care assessment of collagenase activity, providing real-time information for clinicians and researchers. By accurately quantifying collagenase activity, healthcare professionals can optimize patient care, improve treatment outcomes, and contribute to the understanding and management of joint-related disorders. Further research and validation are necessary to establish the full potential of this rapid collagenase activity detection method in clinical practice.

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利用滑膜液样本验证基于荧光淬灭明胶的胶原酶活性快速检测技术。
背景:在关节健康和疾病管理领域,测量胶原酶活性至关重要。胶原蛋白酶是一种负责降解胶原蛋白的酶,在维持关节中胶原蛋白合成和分解之间的平衡方面起着至关重要的作用。胶原酶活性失调会导致关节组织退化,引发类风湿性关节炎和骨关节炎等疾病。开发测量胶原酶活性的方法对于诊断、疾病严重程度评估、治疗监测和确定治疗目标至关重要:本研究旨在利用滑液样本验证胶原酶活性快速检测技术。首先进行了抗体微阵列分析,以量化关节中主要胶原酶基质金属蛋白酶-9(MMP-9)的水平。随后,利用荧光测量法开发的明胶检测法确定胶原酶的活性。MMP-9 的存在与胶原酶活性之间存在明显的相关性。此外,初步估计检测下限和检测上限分别为 8 纳克/毫升和 48 纳克/毫升:所开发的技术提供了一种潜在的胶原酶活性护理点评估方法,为临床医生和研究人员提供了实时信息。通过准确量化胶原蛋白酶的活性,医护人员可以优化患者护理、改善治疗效果,并促进对关节相关疾病的理解和管理。要充分发挥这种胶原蛋白酶活性快速检测方法在临床实践中的潜力,还需要进一步的研究和验证。
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来源期刊
BMC Biotechnology
BMC Biotechnology 工程技术-生物工程与应用微生物
CiteScore
6.60
自引率
0.00%
发文量
34
审稿时长
2 months
期刊介绍: BMC Biotechnology is an open access, peer-reviewed journal that considers articles on the manipulation of biological macromolecules or organisms for use in experimental procedures, cellular and tissue engineering or in the pharmaceutical, agricultural biotechnology and allied industries.
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