Nuclear factors NF-YC3 and NF-YBs positively regulate arbuscular mycorrhizal symbiosis in tomato.

IF 6.5 1区 生物学 Q1 PLANT SCIENCES Plant Physiology Pub Date : 2024-11-04 DOI:10.1093/plphys/kiae381
Heng Chien, Ting-Yu Kuo, Ching-Hung Yao, Yi-Ru Su, Yu-Ting Chang, Zheng-Lin Guo, Kai-Chieh Chang, Yu-Heng Hsieh, Shu-Yi Yang
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Abstract

The involvement of nuclear factor Y (NF-Y) in transcriptional reprogramming during arbuscular mycorrhizal symbiosis has been demonstrated in several plant species. However, a comprehensive picture is lacking. We showed that the spatial expression of NF-YC3 was observed in cortical cells containing arbuscules via the cis-regulatory element GCC boxes. Moreover, the NF-YC3 promoter was transactivated by the combination of CYCLOPS and autoactive calcium and calmodulin-dependent kinase (CCaMK) via GCC boxes. Knockdown of NF-YC3 significantly reduced the abundance of all intraradical fungal structures and affected arbuscule size. BCP1, SbtM1, and WRI5a, whose expression associated with NF-YC3 levels, might be downstream of NF-YC3. NF-YC3 interacted with NF-YB3a, NF-YB5c, or NF-YB3b, in yeast (Saccharomyces cerevisiae) and in planta, and interacted with NF-YA3a in yeast. Spatial expression of 3 NF-YBs was observed in all cell layers of roots under both mock and mycorrhizal conditions. Simultaneous knockdown of 3 NF-YBs, but not individually, reduced the fungal colonization level, suggesting that there might be functional redundancy of NF-YBs to regulate AM symbiosis. Collectively, our data suggest that NF-YC3 and NF-YBs positively regulate AM symbiosis in tomato, and arbuscule-related NF-YC3 may be an important downstream gene of the common symbiosis signaling pathway.

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核因子NF-YC3和NF-YBs对番茄的丛枝菌根共生具有正向调节作用。
在多个植物物种中,核因子 Y(NF-Y)参与了树根菌根共生过程中的转录重编程。然而,目前还缺乏全面的研究。我们发现,NF-YC3 通过顺式调控元件 GCC 盒在含有假根的皮层细胞中进行空间表达。此外,NF-YC3 启动子通过 GCC 框被 CYCLOPS 和自身活性钙及钙调素依赖性激酶(CCaMK)联合转激活。敲除 NF-YC3 会显著降低所有侧内真菌结构的丰度,并影响轴丝的大小。BCP1、SbtM1和WRI5a的表达与NF-YC3水平相关,它们可能是NF-YC3的下游。在酵母(酿酒酵母)和植物中,NF-YC3 与 NF-YB3a、NF-YB5c 或 NF-YB3b 相互作用,在酵母中与 NF-YA3a 相互作用。在模拟和菌根条件下,根的所有细胞层都观察到了三种 NF-YB 的空间表达。同时敲除三个 NF-YBs 而不是单独敲除,会降低真菌的定殖水平,这表明 NF-YBs 在调控 AM 共生方面可能存在功能冗余。总之,我们的数据表明,NF-YC3和NF-YBs对番茄的AM共生有正向调控作用,而与假根相关的NF-YC3可能是共生信号通路的一个重要下游基因。
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来源期刊
Plant Physiology
Plant Physiology 生物-植物科学
CiteScore
12.20
自引率
5.40%
发文量
535
审稿时长
2.3 months
期刊介绍: Plant Physiology® is a distinguished and highly respected journal with a rich history dating back to its establishment in 1926. It stands as a leading international publication in the field of plant biology, covering a comprehensive range of topics from the molecular and structural aspects of plant life to systems biology and ecophysiology. Recognized as the most highly cited journal in plant sciences, Plant Physiology® is a testament to its commitment to excellence and the dissemination of groundbreaking research. As the official publication of the American Society of Plant Biologists, Plant Physiology® upholds rigorous peer-review standards, ensuring that the scientific community receives the highest quality research. The journal releases 12 issues annually, providing a steady stream of new findings and insights to its readership.
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