Glucose biosensing based on glucose oxidase immobilization on carboxymethyl chitosan/polyaniline/multi-walled carbon nanotubes nanocomposite

IF 4.3 3区材料科学 Q2 MATERIALS SCIENCE, COATINGS & FILMS Diamond and Related Materials Pub Date : 2024-07-18 DOI:10.1016/j.diamond.2024.111423

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Abstract

A novel electrochemical glucose biosensor was fabricated using the casting technique of multi-walled carbon nanotubes (MWCNT)/polyaniline (PANI)/carboxymethyl chitosan (CCs) as an appropriate matrix for glucose oxidase (GOx) immobilization. The differential pulse voltammetry (DPV) study results demonstrated a wide linear range of glucose concentrations from 10 nM to 10 μM, with a limit of detection (LOD) of 1.41 μM and a sensitivity of 1791 μA mM⁻¹ cm⁻². Additionally, cyclic voltammetry (CV) data analysis yielded a heterogeneous rate constant (ks) of 0.3 s⁻¹ and an apparent Michaelis-Menten constant ( $k_{m}^{app}$ ) of 18 nM. Over a 30-day period, the biosensor exhibited exceptional repeatability and stability, maintaining approximately 84.58 % of its initial performance for storage stability at 4 °C and 82.13 % for functional stability. Moreover, the reproducibility, interference resistance, and overall performance of the biosensor were assessed, demonstrating its capability to accurately measure glucose levels in human serum with a relative standard deviation (RSD) of <5 %.

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基于固定在羧甲基壳聚糖/聚苯胺/多壁纳米碳管纳米复合材料上的葡萄糖氧化酶的葡萄糖生物传感技术

利用多壁碳纳米管（MWCNT）/聚苯胺（PANI）/羧甲基壳聚糖（CCs）的浇铸技术作为固定葡萄糖氧化酶（GOx）的合适基质，制备了一种新型电化学葡萄糖生物传感器。微分脉冲伏安法（DPV）研究结果表明，葡萄糖浓度的线性范围很宽，从 10 nM 到 10 μM，检测限（LOD）为 1.41 μM，灵敏度为 1791 μA mM-1 cm-2。此外，循环伏安法（CV）数据分析得出的异质速率常数（ks）为 0.3 s-1，表观迈克尔-门顿常数（kmapp）为 18 nM。在 30 天的时间里，该生物传感器表现出卓越的可重复性和稳定性，在 4 °C 的储存稳定性和功能稳定性方面分别保持了约 84.58% 和 82.13% 的初始性能。此外，还对生物传感器的重现性、抗干扰性和整体性能进行了评估，结果表明它能够准确测量人血清中的葡萄糖水平，相对标准偏差 (RSD) 为 5%。

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来源期刊

Diamond and Related Materials 工程技术-材料科学：综合

CiteScore

6.00

自引率

14.60%

发文量

702

审稿时长

2.1 months

期刊介绍： DRM is a leading international journal that publishes new fundamental and applied research on all forms of diamond, the integration of diamond with other advanced materials and development of technologies exploiting diamond. The synthesis, characterization and processing of single crystal diamond, polycrystalline films, nanodiamond powders and heterostructures with other advanced materials are encouraged topics for technical and review articles. In addition to diamond, the journal publishes manuscripts on the synthesis, characterization and application of other related materials including diamond-like carbons, carbon nanotubes, graphene, and boron and carbon nitrides. Articles are sought on the chemical functionalization of diamond and related materials as well as their use in electrochemistry, energy storage and conversion, chemical and biological sensing, imaging, thermal management, photonic and quantum applications, electron emission and electronic devices. The International Conference on Diamond and Carbon Materials has evolved into the largest and most well attended forum in the field of diamond, providing a forum to showcase the latest results in the science and technology of diamond and other carbon materials such as carbon nanotubes, graphene, and diamond-like carbon. Run annually in association with Diamond and Related Materials the conference provides junior and established researchers the opportunity to exchange the latest results ranging from fundamental physical and chemical concepts to applied research focusing on the next generation carbon-based devices.