Screening of Bovine Coronavirus Multiepitope Vaccine Candidates: An Immunoinformatics Approach

IF 3.5 2区 农林科学 Q2 INFECTIOUS DISEASES Transboundary and Emerging Diseases Pub Date : 2024-07-18 DOI:10.1155/2024/5986893
Qian Jiang, Zhigang Ma, Fang Min, Xiaojun Ding, Yumeng Liang, Jinquan Wang, Lu Liu, Na Li, Yawei Sun, Qi Zhong, Gang Yao, Xuelian Ma
{"title":"Screening of Bovine Coronavirus Multiepitope Vaccine Candidates: An Immunoinformatics Approach","authors":"Qian Jiang,&nbsp;Zhigang Ma,&nbsp;Fang Min,&nbsp;Xiaojun Ding,&nbsp;Yumeng Liang,&nbsp;Jinquan Wang,&nbsp;Lu Liu,&nbsp;Na Li,&nbsp;Yawei Sun,&nbsp;Qi Zhong,&nbsp;Gang Yao,&nbsp;Xuelian Ma","doi":"10.1155/2024/5986893","DOIUrl":null,"url":null,"abstract":"<div>\n <p>Bovine coronavirus (BCoV) is a causative agent of enteric and respiratory disease in cattle. BCoV has been reported to cause a variety of animal diseases and is closely related to human coronaviruses; moreover, it has attracted extensive attention from both cattle farmers and researchers. With the rise of BCoV, a vaccine that is prophylactic and immunotherapeutic has to be utilized for a preemptive and adroit therapeutic approach. The aim of this study was to develop a novel multiepitope-based BCoV vaccine that can induce an immune response using a silicon reverse vaccinology approach. In this study, an immunoinformatics approach was employed to identify potential vaccine targets against BCoV, and four candidate antigen proteins were selected to predict B-cell and T-cell epitopes. To identify dominant epitopes, we employed a variety of bioinformatics techniques, including antigenicity prediction, immunogenicity assessment, allergenicity analysis, conservative analysis, and toxicity assessment. Finally, six multiepitope vaccines were developed using dominant epitopes, suitable adjuvants, Pan HLADR—binding epitope (PADRE), and linkers. Then based on the antigenicity score, solubility analysis, allergenicity evaluation, physicochemical property assessment, and tertiary structure verification score, construct 6 was selected as the best candidate vaccine; it was named CY. Molecular modeling and structural validation ensured the high-quality 3D structure of construct CY. The immunogenicity and complex stability of the vaccine were evaluated by molecular docking and kinetic simulation. In silicon clones, the BCoV vaccine had high levels of gene expression in the insect expression system. These results may contribute to the development of experimental BCoV vaccines with higher potency and safety.</p>\n </div>","PeriodicalId":234,"journal":{"name":"Transboundary and Emerging Diseases","volume":"2024 1","pages":""},"PeriodicalIF":3.5000,"publicationDate":"2024-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/5986893","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Transboundary and Emerging Diseases","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1155/2024/5986893","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
引用次数: 0

Abstract

Bovine coronavirus (BCoV) is a causative agent of enteric and respiratory disease in cattle. BCoV has been reported to cause a variety of animal diseases and is closely related to human coronaviruses; moreover, it has attracted extensive attention from both cattle farmers and researchers. With the rise of BCoV, a vaccine that is prophylactic and immunotherapeutic has to be utilized for a preemptive and adroit therapeutic approach. The aim of this study was to develop a novel multiepitope-based BCoV vaccine that can induce an immune response using a silicon reverse vaccinology approach. In this study, an immunoinformatics approach was employed to identify potential vaccine targets against BCoV, and four candidate antigen proteins were selected to predict B-cell and T-cell epitopes. To identify dominant epitopes, we employed a variety of bioinformatics techniques, including antigenicity prediction, immunogenicity assessment, allergenicity analysis, conservative analysis, and toxicity assessment. Finally, six multiepitope vaccines were developed using dominant epitopes, suitable adjuvants, Pan HLADR—binding epitope (PADRE), and linkers. Then based on the antigenicity score, solubility analysis, allergenicity evaluation, physicochemical property assessment, and tertiary structure verification score, construct 6 was selected as the best candidate vaccine; it was named CY. Molecular modeling and structural validation ensured the high-quality 3D structure of construct CY. The immunogenicity and complex stability of the vaccine were evaluated by molecular docking and kinetic simulation. In silicon clones, the BCoV vaccine had high levels of gene expression in the insect expression system. These results may contribute to the development of experimental BCoV vaccines with higher potency and safety.

Abstract Image

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
牛冠状病毒多位点候选疫苗的筛选:免疫信息学方法
牛冠状病毒(BCoV)是牛肠道和呼吸道疾病的病原体。据报道,BCoV 可引起多种动物疾病,与人类冠状病毒关系密切;此外,它还引起了养牛场主和研究人员的广泛关注。随着 BCoV 的兴起,必须利用具有预防和免疫治疗作用的疫苗,以达到先发制人和巧妙治疗的目的。本研究的目的是利用硅反向疫苗学方法开发一种新型多皮层 BCoV 疫苗,该疫苗可诱导免疫应答。本研究采用了一种免疫信息学方法来识别针对 BCoV 的潜在疫苗靶点,并选择了四种候选抗原蛋白来预测 B 细胞和 T 细胞表位。为了确定优势表位,我们采用了多种生物信息学技术,包括抗原性预测、免疫原性评估、过敏性分析、保守性分析和毒性评估。最后,我们利用优势表位、合适的佐剂、Pan HLADR 结合表位(PADRE)和连接体开发出了六种多表位疫苗。然后根据抗原性评分、溶解性分析、过敏性评价、理化性质评估和三级结构验证评分,选择了构建6作为最佳候选疫苗,并将其命名为CY。分子建模和结构验证确保了构建质粒 CY 的高质量三维结构。分子对接和动力学模拟评估了疫苗的免疫原性和复合物稳定性。在硅克隆中,巴科病毒疫苗在昆虫表达系统中具有高水平的基因表达。这些结果可能有助于开发具有更高效力和安全性的实验性 BCoV 疫苗。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Transboundary and Emerging Diseases
Transboundary and Emerging Diseases 农林科学-传染病学
CiteScore
8.90
自引率
9.30%
发文量
350
审稿时长
1 months
期刊介绍: Transboundary and Emerging Diseases brings together in one place the latest research on infectious diseases considered to hold the greatest economic threat to animals and humans worldwide. The journal provides a venue for global research on their diagnosis, prevention and management, and for papers on public health, pathogenesis, epidemiology, statistical modeling, diagnostics, biosecurity issues, genomics, vaccine development and rapid communication of new outbreaks. Papers should include timely research approaches using state-of-the-art technologies. The editors encourage papers adopting a science-based approach on socio-economic and environmental factors influencing the management of the bio-security threat posed by these diseases, including risk analysis and disease spread modeling. Preference will be given to communications focusing on novel science-based approaches to controlling transboundary and emerging diseases. The following topics are generally considered out-of-scope, but decisions are made on a case-by-case basis (for example, studies on cryptic wildlife populations, and those on potential species extinctions): Pathogen discovery: a common pathogen newly recognised in a specific country, or a new pathogen or genetic sequence for which there is little context about — or insights regarding — its emergence or spread. Prevalence estimation surveys and risk factor studies based on survey (rather than longitudinal) methodology, except when such studies are unique. Surveys of knowledge, attitudes and practices are within scope. Diagnostic test development if not accompanied by robust sensitivity and specificity estimation from field studies. Studies focused only on laboratory methods in which relevance to disease emergence and spread is not obvious or can not be inferred (“pure research” type studies). Narrative literature reviews which do not generate new knowledge. Systematic and scoping reviews, and meta-analyses are within scope.
期刊最新文献
Comparative Prevalence Estimation and Phylogenetic Analysis of Novel Porcine Parvoviruses (PPV2–7) in Hungarian Pig Herds A Systematic Review and Meta-Analysis of the Efficacy of Biosecurity in Disease Prevention and Control in Livestock Farms in Africa Methods to Estimate the Between-Population Level Effective Reproductive Number for Infectious Disease Epidemics: Foot-And-Mouth Disease (FMD) in Vietnam Isolation and Genomic Characteristics of a Novel Pathogenicity Type I Feline Coronavirus in Mainland China Molecular and In Vivo Characterization of the High Pathogenicity H7N6 Avian Influenza Virus That Emerged in South African Poultry in 2023
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1