The gene CmPYL6 strongly contributes to cold tolerance in oriental melon

IF 3.6 3区 生物学 Q1 PLANT SCIENCES Plant Biology Pub Date : 2024-07-19 DOI:10.1111/plb.13692
W. Liu, Y. Lv, L. Zhang, Y. Jiang, S. Liu, Z. Wang, J. Zhang, M. He
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CmPYL6 基因对东方甜瓜的耐寒性有很大影响。
目前简单粗放的设施使中国甜瓜生产在淡季栽培期间更容易受到冷胁迫。ABA信号通路是培育耐寒甜瓜的重要目标。利用耐寒的330号和感寒的410号东方甜瓜基因型分析了ABA与耐寒性的关系。根据序列比对和系统进化分析,从甜瓜基因组数据库中鉴定出12个ABA受体CmPYLs。利用拟南芥 330 号的 VIGS 和拟南芥 WT 的过表达,分析了 CmPYL6 在耐寒中的基因功能。共有 12 个 CmPYL 成员含有代表性结构域和保守位点。在冷处理条件下,330号幼苗的电解质渗漏和MDA含量低于410号幼苗,ABA含量和CmPYL6表达量高于410号幼苗。外源施用 ABA 会上调 CmPYL6 的表达,增强两种基因型的耐寒性,而抑制 ABA 的积累则会降低 CmPYL6 的表达和两种基因型的耐寒性。CmPYL6沉默的330号幼苗耐寒性降低,电解质渗漏和MDA含量增加,脯氨酸和可溶性糖含量有限;而CmPYL6过表达的转基因拟南芥植株耐寒性增强,电解质渗漏和MDA含量有限,脯氨酸和可溶性糖含量增加。CmPYL6基因可能是调控东方甜瓜耐寒性的重要ABA受体。我们的研究为提高东方甜瓜耐寒性的育种提供了一个方向。
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来源期刊
Plant Biology
Plant Biology 生物-植物科学
CiteScore
8.20
自引率
2.60%
发文量
109
审稿时长
3 months
期刊介绍: Plant Biology is an international journal of broad scope bringing together the different subdisciplines, such as physiology, molecular biology, cell biology, development, genetics, systematics, ecology, evolution, ecophysiology, plant-microbe interactions, and mycology. Plant Biology publishes original problem-oriented full-length research papers, short research papers, and review articles. Discussion of hot topics and provocative opinion articles are published under the heading Acute Views. From a multidisciplinary perspective, Plant Biology will provide a platform for publication, information and debate, encompassing all areas which fall within the scope of plant science.
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