Cuticle development and the underlying transcriptome-metabolome associations during early seedling establishment.

IF 5.6 2区 生物学 Q1 PLANT SCIENCES Journal of Experimental Botany Pub Date : 2024-10-30 DOI:10.1093/jxb/erae311
Keting Chen, Rupam Kumar Bhunia, Matthew M Wendt, Grace Campidilli, Colton McNinch, Ahmed Hassan, Ling Li, Basil J Nikolau, Marna D Yandeau-Nelson
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Abstract

The plant cuticle is a complex extracellular lipid barrier that has multiple protective functions. This study investigated cuticle deposition by integrating metabolomics and transcriptomics data gathered from six different maize seedling organs of four genotypes, the inbred lines B73 and Mo17, and their reciprocal hybrids. These datasets captured the developmental transition of the seedling from heterotrophic skotomorphogenic growth to autotrophic photomorphogenic growth, a transition that is highly vulnerable to environmental stresses. Statistical interrogation of these data revealed that the predominant determinant of cuticle composition is seedling organ type, whereas the seedling genotype has a smaller effect on this phenotype. Gene-to-metabolite associations assessed by integrated statistical analyses identified three gene networks associated with the deposition of different elements of the cuticle: cuticular waxes; monomers of lipidized cell wall biopolymers, including cutin and suberin; and both of these elements. These gene networks reveal three metabolic programs that appear to support cuticle deposition, including processes of chloroplast biogenesis, lipid metabolism, and molecular regulation (e.g. transcription factors, post-translational regulators, and phytohormones). This study demonstrates the wider physiological metabolic context that can determine cuticle deposition and lays the groundwork for new targets for modulating the properties of this protective barrier.

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幼苗期角质层的发育及其转录组和代谢组之间的联系
植物角质层是一种复杂的细胞外脂质屏障,具有多种保护功能。我们通过整合从四个基因型的六个不同玉米幼苗器官、近交系 B73 和 Mo17 以及它们的互交杂交种收集的代谢组学和转录组学数据,研究了角质层的沉积。这些数据集捕捉到了幼苗从异养性脱肥生长向自养性光生长的发育转变过程,而这一转变过程极易受到环境胁迫的影响。对这些数据的统计分析显示,决定角质层组成的主要因素是幼苗器官类型,而幼苗基因型对这一表型的影响较小。通过综合统计分析评估基因与代谢物之间的关联,发现有三个基因网络与角质层不同成分的沉积有关:a)角质蜡;b)脂化细胞壁生物聚合物的单体,包括角质素和单宁;c)这两种成分。这些基因网络揭示了支持角质层沉积的三种代谢程序,包括叶绿体生物发生过程、脂质代谢过程和分子调控过程(如转录因子、翻译后调节因子和植物激素)。这项研究展示了决定角质层沉积的更广泛的生理代谢背景,并为调节这一保护屏障特性的新目标奠定了基础。
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来源期刊
Journal of Experimental Botany
Journal of Experimental Botany 生物-植物科学
CiteScore
12.30
自引率
4.30%
发文量
450
审稿时长
1.9 months
期刊介绍: The Journal of Experimental Botany publishes high-quality primary research and review papers in the plant sciences. These papers cover a range of disciplines from molecular and cellular physiology and biochemistry through whole plant physiology to community physiology. Full-length primary papers should contribute to our understanding of how plants develop and function, and should provide new insights into biological processes. The journal will not publish purely descriptive papers or papers that report a well-known process in a species in which the process has not been identified previously. Articles should be concise and generally limited to 10 printed pages.
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