Alena Patnaik, Priyanka Mishra, Anish Dash, Madhusmita Panigrahy, Kishore C S Panigrahi
GIGANTEA is a multifaceted plant-specific protein that originated in a streptophyte ancestor. The current known functions of GI include circadian clock control, light signalling, flowering time regulation, stomata response, chloroplast biogenesis, accumulation of anthocyanin, chlorophyll, and starch, phytohormone signalling, senescence and response to drought, salt, and oxidative stress. Six decades since its discovery, no functional domains have been defined, and its mechanism of action is still not well-characterised. In this review, we explore the functional evolution of GI to distinguish between ancestral and more recently acquired roles. GI integrated itself into various existing signalling pathways of the circadian clock, blue light, photoperiod, and osmotic and oxidative stress response. It also evolved parallelly to acquire new functions for chloroplast accumulation, red light signalling and anthocyanin production. In this review, we have encapsulated the known mechanisms of various biological functions of GI. Additionally, this manuscript will throw light on the evolution of GI in plant lineage.
GIGANTEA 是一种多方面的植物特异性蛋白质,起源于链格植物的祖先。目前已知的 GI 功能包括昼夜节律控制、光信号、花期调节、气孔反应、叶绿体生物发生、花青素、叶绿素和淀粉的积累、植物激素信号、衰老以及对干旱、盐和氧化应激的反应。自其被发现以来的六十年间,尚未确定其功能域,其作用机制也仍未得到很好的描述。在这篇综述中,我们探讨了 GI 的功能演变,以区分其祖先的作用和最近获得的作用。GI 将自身整合到昼夜节律、蓝光、光周期以及渗透和氧化应激反应的各种现有信号通路中。同时,它还在进化过程中获得了叶绿体积累、红光信号和花青素生产等新功能。在这篇综述中,我们概括了 GI 各种生物功能的已知机制。此外,本手稿还将揭示 GI 在植物品系中的进化过程。
{"title":"Evolution of light-dependent functions of GIGANTEA.","authors":"Alena Patnaik, Priyanka Mishra, Anish Dash, Madhusmita Panigrahy, Kishore C S Panigrahi","doi":"10.1093/jxb/erae441","DOIUrl":"https://doi.org/10.1093/jxb/erae441","url":null,"abstract":"<p><p>GIGANTEA is a multifaceted plant-specific protein that originated in a streptophyte ancestor. The current known functions of GI include circadian clock control, light signalling, flowering time regulation, stomata response, chloroplast biogenesis, accumulation of anthocyanin, chlorophyll, and starch, phytohormone signalling, senescence and response to drought, salt, and oxidative stress. Six decades since its discovery, no functional domains have been defined, and its mechanism of action is still not well-characterised. In this review, we explore the functional evolution of GI to distinguish between ancestral and more recently acquired roles. GI integrated itself into various existing signalling pathways of the circadian clock, blue light, photoperiod, and osmotic and oxidative stress response. It also evolved parallelly to acquire new functions for chloroplast accumulation, red light signalling and anthocyanin production. In this review, we have encapsulated the known mechanisms of various biological functions of GI. Additionally, this manuscript will throw light on the evolution of GI in plant lineage.</p>","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142583378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The inducer β-aminobutyric acid (BABA) is capable of immune response in various plants. However, the specific mitogen-activated protein kinase (MAPK) cascade involved in BABA-induced resistance (BABA-IR) has not yet been elucidated. Here, peach fruits treated with the BABA exhibited a pattern-triggered immunity (PTI) defense against Rhizopus stolonifer, accompanied by the generation of reactive oxygen species (ROS) and activation of MAPK cascade. Transcriptome sequencing suggested a total of fifteen PpMAPKKK/PpMAPKK/PpMAPK genes involved in BABA-IR in peach fruit. Further qRT-PCR analysis showed that the transcript profiles of PpMAPKKK3, PpMAPKK5 and PpMAPK1 were obviously potentiated. Subsequently, yeast two-hybrid (Y2H), luciferase complementation imaging (LCI), pull-down and in vitro phosphorylation assays were conducted to characterize the complete MAPK cascade (PpMAPKKK3-PpMAPKK5-PpMAPK1) involved in peach fruit. Moreover, the downstream events of MAPK1 include the involvement of SNARE13 and the corresponding NPR1-responsive defense. Single silencing of MAPKKK3, MAPKK5 or MAPK1 and double silencing of MAPKKK3/MAPKK5 or MAPKK5/MAPK1 resulted in enhanced susceptibility to the fungus R. stolonifer in mutants and attenuated salicylic acid (SA)-dependent defense gene expression; in contrast, the homologous or heterologous overexpression of PpSNARE13 in peach fruit or Arabidopsis led to an enhanced SA pool and elevated expression of PR genes. Reciprocally, the ppsnare13cas9 mutants are generally compromised in the priming of SA-dependent resistance. Therefore, the MAPKKK3-MAPKK5-MAPK1 cascade contributes to PTI signal transduction in BABA-elicited peach fruit, by combination with downstream events such as SNARE13, NPR1, and SA-dependent signaling.
诱导剂 β-氨基丁酸(BABA)能对多种植物产生免疫反应。然而,参与 BABA 诱导的抗性(BABA-IR)的特定丝裂原活化蛋白激酶(MAPK)级联尚未阐明。在本文中,经 BABA 处理的桃果对匍匐茎根瘤菌(Rhizopus stolonifer)表现出模式触发免疫(PTI)防御,并伴随着活性氧(ROS)的产生和 MAPK 级联的激活。转录组测序表明,共有 15 个 PpMAPKK/PpMAPKK/PpMAPK 基因参与了桃果中的 BABA-IR 过程。进一步的 qRT-PCR 分析表明,PpMAPKKK3、PpMAPKK5 和 PpMAPK1 的转录本特征明显增强。随后,通过酵母双杂交(Y2H)、荧光素酶互补成像(LCI)、牵引和体外磷酸化分析,确定了桃果实中涉及的完整 MAPK 级联(PpMAPKKK3-PpMAPKK5-PpMAPK1)。此外,MAPK1 的下游事件包括 SNARE13 和相应的 NPR1 响应防御的参与。MAPKKK3、MAPKK5或MAPK1的单沉默以及MAPKKK3/MAPKK5或MAPKK5/MAPK1的双沉默导致突变体对真菌R. stolonifer的易感性增强,依赖水杨酸(SA)的防御基因表达减弱;相反,在桃果实或拟南芥中同源或异源过表达PpSNARE13会导致SA池增强和PR基因表达升高。与此相对应,ppsnare13cas9 突变体在启动 SA 依赖性抗性方面普遍受到影响。因此,MAPKK3-MAPKK5-MAPK1 级联与 SNARE13、NPR1 和 SA 依赖性信号转导等下游事件相结合,有助于 BABA 激发的桃果实中的 PTI 信号转导。
{"title":"β-aminobutyric acid-induced resistance in postharvest peach fruit involves interaction between the MAPK cascade and SNARE13 protein in salicylic acid-dependent pathway.","authors":"Chunhong Li, Kaituo Wang, Changyi Lei, Yanyu Zou, Sisi Yang, Fei Xiang, Meilin Li, Yonghua Zheng","doi":"10.1093/jxb/erae448","DOIUrl":"https://doi.org/10.1093/jxb/erae448","url":null,"abstract":"<p><p>The inducer β-aminobutyric acid (BABA) is capable of immune response in various plants. However, the specific mitogen-activated protein kinase (MAPK) cascade involved in BABA-induced resistance (BABA-IR) has not yet been elucidated. Here, peach fruits treated with the BABA exhibited a pattern-triggered immunity (PTI) defense against Rhizopus stolonifer, accompanied by the generation of reactive oxygen species (ROS) and activation of MAPK cascade. Transcriptome sequencing suggested a total of fifteen PpMAPKKK/PpMAPKK/PpMAPK genes involved in BABA-IR in peach fruit. Further qRT-PCR analysis showed that the transcript profiles of PpMAPKKK3, PpMAPKK5 and PpMAPK1 were obviously potentiated. Subsequently, yeast two-hybrid (Y2H), luciferase complementation imaging (LCI), pull-down and in vitro phosphorylation assays were conducted to characterize the complete MAPK cascade (PpMAPKKK3-PpMAPKK5-PpMAPK1) involved in peach fruit. Moreover, the downstream events of MAPK1 include the involvement of SNARE13 and the corresponding NPR1-responsive defense. Single silencing of MAPKKK3, MAPKK5 or MAPK1 and double silencing of MAPKKK3/MAPKK5 or MAPKK5/MAPK1 resulted in enhanced susceptibility to the fungus R. stolonifer in mutants and attenuated salicylic acid (SA)-dependent defense gene expression; in contrast, the homologous or heterologous overexpression of PpSNARE13 in peach fruit or Arabidopsis led to an enhanced SA pool and elevated expression of PR genes. Reciprocally, the ppsnare13cas9 mutants are generally compromised in the priming of SA-dependent resistance. Therefore, the MAPKKK3-MAPKK5-MAPK1 cascade contributes to PTI signal transduction in BABA-elicited peach fruit, by combination with downstream events such as SNARE13, NPR1, and SA-dependent signaling.</p>","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142576333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Phosphorus (P) is a quintessential macronutrient utilized by plants to support various metabolic processes during growth and development. Recent studies have revealed the pivotal role of inositol hexa-kis/pyrophosphate (InsP6-8), the derivatives of Myo-inositol (MI), in facilitating the interaction between SYG1/PHO81/XPR1 (SPX) and Phosphate starvation response (PHR) proteins. Myo-inositol phosphate synthase (MIPS) catalyzes the first committed step in MI biosynthesis. Although the role of MIPS genes in mediating stress responses in plants is well elucidated, its role in phosphate (Pi) deficiency remains largely unexplored. This study demonstrates that out of the five MIPS genes encoded by the tomato genome, only SlMIPS2 is sharply induced at an early stage of Pi starvation in tomato seedlings. Silencing of SlMIPS2 led to improved seedling growth with enhanced total soluble Pi and total P levels in the silenced plants under high Pi availability. SlMIPS2 silencing also caused a significant reduction in MI and InsP6 content in the tomato seedlings. These seedlings with depleted InsP6 levels accumulated lower levels of SlSPX2 protein. In contrast, stabilized SlPHL1 levels were noticed in these plants, directly implicating this transcription factor in activating phosphate starvation inducible (PSI) genes in the SlMIPS2-silenced seedlings, even under high Pi conditions. The results assign a novel role to SlMIPS2 in regulating cellular InsP6 levels and SPX-PHR interactions to control Pi homeostasis in tomato seedlings.
磷(P)是植物在生长发育过程中用于支持各种代谢过程的重要宏量营养元素。最近的研究揭示了肌醇六-基/焦磷酸(InsP6-8)--肌醇(MI)的衍生物--在促进 SYG1/PHO81/XPR1 (SPX) 和磷酸盐饥饿反应(PHR)蛋白之间相互作用中的关键作用。肌醇磷酸合成酶(MIPS)催化 MI 生物合成的第一步。尽管 MIPS 基因在介导植物胁迫响应中的作用已被充分阐明,但其在磷酸盐(Pi)缺乏中的作用在很大程度上仍未被探索。本研究表明,在番茄基因组编码的五个 MIPS 基因中,只有 SlMIPS2 在番茄幼苗 Pi 饥饿的早期阶段被急剧诱导。沉默 SlMIPS2 可改善秧苗的生长,在高 Pi 供应条件下,被沉默植株的总可溶性 Pi 和总 P 水平均有所提高。Silencing SlMIPS2 还导致番茄幼苗中 MI 和 InsP6 含量显著降低。这些 InsP6 含量减少的秧苗积累的 SlSPX2 蛋白水平较低。与此相反,这些植株中的 SlPHL1 水平趋于稳定,这直接表明即使在高 Pi 条件下,SlMIPS2 沉默的秧苗中的该转录因子也能激活磷酸盐饥饿诱导(PSI)基因。结果表明,SlMIPS2 在调控细胞 InsP6 水平和 SPX-PHR 相互作用以控制番茄幼苗的 Pi 平衡方面发挥了新的作用。
{"title":"SlMIPS2, a myo-inositol phosphate synthase gene, regulates phosphate homeostasis by influencing SlPHL1 and SlSPX2 levels in tomato seedlings.","authors":"Abhishek Roychowdhury, Dolly Kaushik, Jayashri Babaji Bhosale, Rajat Srivastava, Rahul Kumar","doi":"10.1093/jxb/erae451","DOIUrl":"https://doi.org/10.1093/jxb/erae451","url":null,"abstract":"<p><p>Phosphorus (P) is a quintessential macronutrient utilized by plants to support various metabolic processes during growth and development. Recent studies have revealed the pivotal role of inositol hexa-kis/pyrophosphate (InsP6-8), the derivatives of Myo-inositol (MI), in facilitating the interaction between SYG1/PHO81/XPR1 (SPX) and Phosphate starvation response (PHR) proteins. Myo-inositol phosphate synthase (MIPS) catalyzes the first committed step in MI biosynthesis. Although the role of MIPS genes in mediating stress responses in plants is well elucidated, its role in phosphate (Pi) deficiency remains largely unexplored. This study demonstrates that out of the five MIPS genes encoded by the tomato genome, only SlMIPS2 is sharply induced at an early stage of Pi starvation in tomato seedlings. Silencing of SlMIPS2 led to improved seedling growth with enhanced total soluble Pi and total P levels in the silenced plants under high Pi availability. SlMIPS2 silencing also caused a significant reduction in MI and InsP6 content in the tomato seedlings. These seedlings with depleted InsP6 levels accumulated lower levels of SlSPX2 protein. In contrast, stabilized SlPHL1 levels were noticed in these plants, directly implicating this transcription factor in activating phosphate starvation inducible (PSI) genes in the SlMIPS2-silenced seedlings, even under high Pi conditions. The results assign a novel role to SlMIPS2 in regulating cellular InsP6 levels and SPX-PHR interactions to control Pi homeostasis in tomato seedlings.</p>","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142564236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The future sustainability of viticulture depends on the development of grapevine cultivars with genetic resistance to diseases such as powdery mildew, downy mildew, and Pierce's disease. Recent advances in grape and pathogen genomics have dramatically improved our approach to durable disease resistance. The availability of diploid genome references for wild species, combined with the ability to phase resistance haplotypes and conduct genome-wide association and expression analyses, has greatly enhanced our ability to dissect genetic resistance loci. This progress is yielding candidate genes that will form the foundation for precise breeding, gene stacking, and genome editing in grape improvement programs. As resistance genes are deployed in vineyards, pathogen populations evolve to adapt and evade these defenses, posing ongoing challenges. Understanding the adaptive mechanisms of grapevine pathogens in response to resistant cultivars is crucial. Grape pathogenomics is advancing rapidly, marked by the sequencing of many pathogen genomes, the discovery of effectors, including the first ones responsible for disease resistance breakdown, and the development of graph-based pangenomes. These advancements offer valuable insights into pathogen evolution and inform strategies for sustainable disease management. Together, these genomic tools and insights are paving the way for developing resilient grapevine varieties, ensuring the long-term sustainability of viticulture.
{"title":"Advances in grape and pathogen genomics toward durable grapevine disease resistance.","authors":"Manon Paineau, Mirella Zaccheo, Mélanie Massonnet, Dario Cantu","doi":"10.1093/jxb/erae450","DOIUrl":"https://doi.org/10.1093/jxb/erae450","url":null,"abstract":"<p><p>The future sustainability of viticulture depends on the development of grapevine cultivars with genetic resistance to diseases such as powdery mildew, downy mildew, and Pierce's disease. Recent advances in grape and pathogen genomics have dramatically improved our approach to durable disease resistance. The availability of diploid genome references for wild species, combined with the ability to phase resistance haplotypes and conduct genome-wide association and expression analyses, has greatly enhanced our ability to dissect genetic resistance loci. This progress is yielding candidate genes that will form the foundation for precise breeding, gene stacking, and genome editing in grape improvement programs. As resistance genes are deployed in vineyards, pathogen populations evolve to adapt and evade these defenses, posing ongoing challenges. Understanding the adaptive mechanisms of grapevine pathogens in response to resistant cultivars is crucial. Grape pathogenomics is advancing rapidly, marked by the sequencing of many pathogen genomes, the discovery of effectors, including the first ones responsible for disease resistance breakdown, and the development of graph-based pangenomes. These advancements offer valuable insights into pathogen evolution and inform strategies for sustainable disease management. Together, these genomic tools and insights are paving the way for developing resilient grapevine varieties, ensuring the long-term sustainability of viticulture.</p>","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142564212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fede Berckx, Thanh Van Nguyen, Rolf Hilker, Daniel Wibberg, Kai Battenberg, Jörn Kalinowski, Alison Berry, Katharina Pawlowski
Frankia cluster-2 strains are diazotrophs that engage in root nodule symbiosis with actinorhizal plants of the Cucurbitales and the Rosales. Previous studies have shown that an assimilated nitrogen source, presumably arginine, is exported to the host in nodules of Datisca glomerata (Cucurbitales), while a different metabolite is exported in the nodules of Ceanothus thyrsiflorus (Rosales). To investigate if an assimilated nitrogen form is commonly exported to the host by cluster-2 strains, and which metabolite would be exported in Ceanothus, we analysed gene expression levels, metabolite profiles, and enzyme activities in nodules. We conclude that the export of assimilated nitrogen in symbiosis seems to be a common feature for Frankia cluster-2 strains, but which source is host-dependent. The export of assimilated ammonium to the host suggests that 2-oxoglutarate is drawn from the TCA cycle at a high rate. This specialised metabolism obviates the need for the reductive branch of the TCA cycle. We found several genes encoding enzymes of the central carbon and nitrogen metabolism were lacking in Frankia cluster-2 genomes: the glyoxylate shunt and succinate semialdehyde dehydrogenase. This led to a linearization of the TCA cycle, and we hypothesize this could explain the low saprotrophic potential of Frankia cluster-2.
{"title":"Host dependent specialized metabolism of nitrogen export in actinorhizal nodules induced by Frankia cluster-2.","authors":"Fede Berckx, Thanh Van Nguyen, Rolf Hilker, Daniel Wibberg, Kai Battenberg, Jörn Kalinowski, Alison Berry, Katharina Pawlowski","doi":"10.1093/jxb/erae446","DOIUrl":"https://doi.org/10.1093/jxb/erae446","url":null,"abstract":"<p><p>Frankia cluster-2 strains are diazotrophs that engage in root nodule symbiosis with actinorhizal plants of the Cucurbitales and the Rosales. Previous studies have shown that an assimilated nitrogen source, presumably arginine, is exported to the host in nodules of Datisca glomerata (Cucurbitales), while a different metabolite is exported in the nodules of Ceanothus thyrsiflorus (Rosales). To investigate if an assimilated nitrogen form is commonly exported to the host by cluster-2 strains, and which metabolite would be exported in Ceanothus, we analysed gene expression levels, metabolite profiles, and enzyme activities in nodules. We conclude that the export of assimilated nitrogen in symbiosis seems to be a common feature for Frankia cluster-2 strains, but which source is host-dependent. The export of assimilated ammonium to the host suggests that 2-oxoglutarate is drawn from the TCA cycle at a high rate. This specialised metabolism obviates the need for the reductive branch of the TCA cycle. We found several genes encoding enzymes of the central carbon and nitrogen metabolism were lacking in Frankia cluster-2 genomes: the glyoxylate shunt and succinate semialdehyde dehydrogenase. This led to a linearization of the TCA cycle, and we hypothesize this could explain the low saprotrophic potential of Frankia cluster-2.</p>","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142564214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Trichomes are specialized hair-like structures in the epidermal cells of the above-ground parts of plants and help to protect them from pests and pathogens, and produce valuable metabolites. Chrysanthemum morifolium, which is used in tea products, has both ornamental and medicinal value; however, it is susceptible to infection by the fungus Alternaria alternata, which can result in substantial economic losses. Increasing the density of glandular trichomes enhances disease resistance and improves the production of medicinal metabolites in chrysanthemums, and jasmonate (JA) is known to promote the formation of trichomes in various plants. However, it remains unclear whether glandular trichomes in chrysanthemums are regulated by JA. In addition, grafting, a technique that can improve plant resistance to biotic stresses, has been poorly examined for its impact on glandular trichomes, terpenoids, and disease resistance. In this study, we demonstrate that grafting with Artemisia vulgaris rootstocks improves the resistance of chrysanthemum scions to A. alternata. Heterografted chrysanthemums exhibited higher trichome density and terpenoid content compared to self-grafted counterparts. Transcriptome analysis highlighted the significant role of CmJAZ1-like in disease resistance in heterografted chrysanthemums. Lines overexpressing CmJAZ1-like exhibited sensitivity to A. alternata, and this was characterized by reduced glandular trichome density and limited terpenoid content. Conversely, CmJAZ1-like silenced lines exhibited resistance to A. alternata and showed increased glandular trichome density and terpenoid content. Higher JA content was found in the heterografted chrysanthemum scions compared to self-grafted ones. Furthermore, we established that JA promoted the development of glandular trichomes and the synthesis of terpenoids while also inducing the degradation of CmJAZ1-like proteins in chrysanthemums. Our findings suggest that higher JA increases trichome density and terpenoid content, thereby enhancing resistance to A. alternata by regulating CmJAZ1-like in heterografted chrysanthemums.
毛状体是植物地上部分表皮细胞中特化的毛状结构,可保护植物免受害虫和病原体的侵害,并产生有价值的代谢物。菊花(Chrysanthemum morifolium)用于茶叶产品,具有观赏和药用价值。然而,菊花易受交替丝核菌(Alternaria alternata)真菌感染,对其生产和使用构成威胁,造成巨大的经济损失。增加腺毛的密度可以增强菊花的抗病性,提高药用代谢物的产量。茉莉酸盐(JA)能促进各种植物腺毛的形成。然而,菊花的腺毛体是否受 JA 的调控仍不清楚。嫁接作为一种提高植物抗生物胁迫能力的技术,其对腺毛体、萜类化合物和抗病性的影响还没有得到充分的探讨。在这项研究中,我们证明了与青蒿砧木嫁接可提高菊花接穗对交替疟原虫的抗性。与自嫁接的菊花相比,异株嫁接的菊花表现出更高的毛状体密度和萜类化合物含量。转录组分析强调了 CmJAZ1-like 在异株嫁接菊花抗病性中的重要作用。过表达 CmJAZ1-like 的品系对交替菊表现出敏感性,其特点是腺毛密度降低,萜类化合物含量有限。相反,沉默株系表现出对交替花叶病毒的抗性,腺毛密度增加,萜类化合物丰富。异株嫁接菊花接穗中的 JA 含量高于自接穗。此外,我们还证实 JA 能促进腺毛体的发育和萜类化合物的合成,同时诱导菊花中 CmJAZ1 类蛋白的降解。这些研究结果表明,较高的 JA 能增加毛状体密度和萜类化合物含量,通过调节异株嫁接菊花中的 CmJAZ1-like 来增强对交替穗霉的抗性。
{"title":"Heterografting enhances chrysanthemum resistance to Alternaria alternata via jasmonate-mediated increases in trichomes and terpenoids.","authors":"Wenjie Li, Qingling Zhan, Yunxiao Guan, Likai Wang, Song Li, Shanhu Zheng, Hongyu Ma, Ye Liu, Lian Ding, Shuang Zhao, Zhenxing Wang, Jiafu Jiang, Weimin Fang, Fadi Chen, Sumei Chen, Zhiyong Guan","doi":"10.1093/jxb/erae212","DOIUrl":"10.1093/jxb/erae212","url":null,"abstract":"<p><p>Trichomes are specialized hair-like structures in the epidermal cells of the above-ground parts of plants and help to protect them from pests and pathogens, and produce valuable metabolites. Chrysanthemum morifolium, which is used in tea products, has both ornamental and medicinal value; however, it is susceptible to infection by the fungus Alternaria alternata, which can result in substantial economic losses. Increasing the density of glandular trichomes enhances disease resistance and improves the production of medicinal metabolites in chrysanthemums, and jasmonate (JA) is known to promote the formation of trichomes in various plants. However, it remains unclear whether glandular trichomes in chrysanthemums are regulated by JA. In addition, grafting, a technique that can improve plant resistance to biotic stresses, has been poorly examined for its impact on glandular trichomes, terpenoids, and disease resistance. In this study, we demonstrate that grafting with Artemisia vulgaris rootstocks improves the resistance of chrysanthemum scions to A. alternata. Heterografted chrysanthemums exhibited higher trichome density and terpenoid content compared to self-grafted counterparts. Transcriptome analysis highlighted the significant role of CmJAZ1-like in disease resistance in heterografted chrysanthemums. Lines overexpressing CmJAZ1-like exhibited sensitivity to A. alternata, and this was characterized by reduced glandular trichome density and limited terpenoid content. Conversely, CmJAZ1-like silenced lines exhibited resistance to A. alternata and showed increased glandular trichome density and terpenoid content. Higher JA content was found in the heterografted chrysanthemum scions compared to self-grafted ones. Furthermore, we established that JA promoted the development of glandular trichomes and the synthesis of terpenoids while also inducing the degradation of CmJAZ1-like proteins in chrysanthemums. Our findings suggest that higher JA increases trichome density and terpenoid content, thereby enhancing resistance to A. alternata by regulating CmJAZ1-like in heterografted chrysanthemums.</p>","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140921957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Vitor F Pinoti, Pedro B Ferreira, Edward J Strini, Greice Lubini, Vanessa Thomé, Joelma O Cruz, Rodrigo Aziani, Andréa C Quiapim, Andressa P A Pinto, Ana Paula U Araujo, Henrique C De Paoli, Maria Cristina S Pranchevicius, Maria Helena S Goldman
Successful plant reproduction depends on the adequate development of floral organs controlled by cell proliferation and other processes. The Stigma/style cell-cycle inhibitor 1 (SCI1) gene regulates cell proliferation and affects the final size of the female reproductive organ. To unravel the molecular mechanism exerted by Nicotiana tabacum SCI1 in cell proliferation control, we searched for its interaction partners through semi-in vivo pull-down experiments, uncovering a cyclin-dependent kinase, NtCDKG;2. Bimolecular fluorescence complementation and co-localization experiments showed that SCI1 interacts with NtCDKG;2 and its cognate NtCyclin L in nucleoli and splicing speckles. The screening of a yeast two-hybrid cDNA library using SCI1 as bait revealed a novel DEAD-box RNA helicase (NtRH35). Interaction between the NtCDKG;2-NtCyclin L complex and NtRH35 is also shown. Subcellular localization experiments showed that SCI1, NtRH35, and the NtCDKG;2-NtCyclin L complex associate with each other within splicing speckles. The yeast two-hybrid screening of NtCDKG;2 and NtRH35 identified the conserved spliceosome components U2a', NF-κB activating protein (NKAP), and CACTIN. This work presents SCI1 and its interactors, the NtCDKG;2-NtCyclin L complex and NtRH35, as new spliceosome-associated proteins. Our findings reveal a network of interactions and indicate that SCI1 may regulate cell proliferation through the splicing process, providing new insights into the intricate molecular pathways governing plant development.
{"title":"SCI1, a flower regulator of cell proliferation, and its partners NtCDKG2 and NtRH35 interact with the splicing machinery.","authors":"Vitor F Pinoti, Pedro B Ferreira, Edward J Strini, Greice Lubini, Vanessa Thomé, Joelma O Cruz, Rodrigo Aziani, Andréa C Quiapim, Andressa P A Pinto, Ana Paula U Araujo, Henrique C De Paoli, Maria Cristina S Pranchevicius, Maria Helena S Goldman","doi":"10.1093/jxb/erae337","DOIUrl":"10.1093/jxb/erae337","url":null,"abstract":"<p><p>Successful plant reproduction depends on the adequate development of floral organs controlled by cell proliferation and other processes. The Stigma/style cell-cycle inhibitor 1 (SCI1) gene regulates cell proliferation and affects the final size of the female reproductive organ. To unravel the molecular mechanism exerted by Nicotiana tabacum SCI1 in cell proliferation control, we searched for its interaction partners through semi-in vivo pull-down experiments, uncovering a cyclin-dependent kinase, NtCDKG;2. Bimolecular fluorescence complementation and co-localization experiments showed that SCI1 interacts with NtCDKG;2 and its cognate NtCyclin L in nucleoli and splicing speckles. The screening of a yeast two-hybrid cDNA library using SCI1 as bait revealed a novel DEAD-box RNA helicase (NtRH35). Interaction between the NtCDKG;2-NtCyclin L complex and NtRH35 is also shown. Subcellular localization experiments showed that SCI1, NtRH35, and the NtCDKG;2-NtCyclin L complex associate with each other within splicing speckles. The yeast two-hybrid screening of NtCDKG;2 and NtRH35 identified the conserved spliceosome components U2a', NF-κB activating protein (NKAP), and CACTIN. This work presents SCI1 and its interactors, the NtCDKG;2-NtCyclin L complex and NtRH35, as new spliceosome-associated proteins. Our findings reveal a network of interactions and indicate that SCI1 may regulate cell proliferation through the splicing process, providing new insights into the intricate molecular pathways governing plant development.</p>","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141901941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Robertsonian translocations made easier.","authors":"Ingo Schubert","doi":"10.1093/jxb/erae349","DOIUrl":"10.1093/jxb/erae349","url":null,"abstract":"","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141916874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Understanding the spatial organization of genomes within chromatin is crucial for deciphering gene regulation. A recently developed CRISPR-dCas9-based genome labeling tool, known as CRISPR-FISH, allows efficient labeling of repetitive sequences. Unlike standard fluorescence in situ hybridization (FISH), CRISPR-FISH eliminates the need for global DNA denaturation, allowing for superior preservation of chromatin structure. Here, we report on further development of the CRISPR-FISH method, which has been enhanced for increased efficiency through the engineering of a recombinant dCas9 protein containing an ALFA-tag. Using an ALFA-tagged dCas9 protein assembled with an Arabidopsis centromere-specific guide RNA, we demonstrate target-specific labeling with a fluorescence-labeled NbALFA nanobody. The dCas9 protein possessing multiple copies of the ALFA-tag, in combination with a minibody and fluorescence-labeled anti-rabbit secondary antibody, resulted in enhanced target-specific signals. The dCas9-ALFA-tag system was also instrumental in live cell imaging of telomeres in Nicotiana benthamiana. This method will further expand the CRISPR imaging toolkit, facilitating a better understanding of genome organization. Furthermore, we report the successful integration of the highly sensitive tyramide signal amplification method with CRISPR-FISH, demonstrating effective labeling of Arabidopsis centromeres.
了解染色质中基因组的空间组织对于破译基因调控至关重要。最近开发的一种基于CRISPR-dCas9的基因组标记工具,即CRISPR-FISH,可以对重复序列进行高效标记。与标准的荧光原位杂交(FISH)不同,CRISPR-FISH 无需对 DNA 进行全局变性,从而能更好地保存染色质结构。在这里,我们报告了CRISPR-FISH方法的进一步发展,该方法通过对含有ALFA标记的重组dCas9蛋白进行工程化而提高了效率。利用 ALFA 标记的 dCas9 蛋白与 A. thaliana 中心粒特异性 gRNA 组装,我们用荧光标记的 NbALFA 纳米抗体展示了靶标特异性标记。具有多个 ALFA 标记拷贝的 dCas9 蛋白与迷你抗体和荧光标记的抗兔二抗结合,增强了靶标特异性信号。dCas9-ALFA-tag 系统还有助于对 N. benthamiana 的端粒进行活细胞成像。这种方法将进一步扩展 CRISPR 成像工具包,有助于更好地了解基因组的组织结构。此外,我们还报告了高灵敏度的泰拉米德信号放大(TSA)方法与 CRISPR-FISH 的成功整合,证明了对 A. thaliana 中心粒的有效标记。
{"title":"The potential of ALFA-tag and tyramide-based fluorescence signal amplification to expand the CRISPR-based DNA imaging toolkit.","authors":"Bhanu Prakash Potlapalli, Jörg Fuchs, Twan Rutten, Armin Meister, Andreas Houben","doi":"10.1093/jxb/erae341","DOIUrl":"10.1093/jxb/erae341","url":null,"abstract":"<p><p>Understanding the spatial organization of genomes within chromatin is crucial for deciphering gene regulation. A recently developed CRISPR-dCas9-based genome labeling tool, known as CRISPR-FISH, allows efficient labeling of repetitive sequences. Unlike standard fluorescence in situ hybridization (FISH), CRISPR-FISH eliminates the need for global DNA denaturation, allowing for superior preservation of chromatin structure. Here, we report on further development of the CRISPR-FISH method, which has been enhanced for increased efficiency through the engineering of a recombinant dCas9 protein containing an ALFA-tag. Using an ALFA-tagged dCas9 protein assembled with an Arabidopsis centromere-specific guide RNA, we demonstrate target-specific labeling with a fluorescence-labeled NbALFA nanobody. The dCas9 protein possessing multiple copies of the ALFA-tag, in combination with a minibody and fluorescence-labeled anti-rabbit secondary antibody, resulted in enhanced target-specific signals. The dCas9-ALFA-tag system was also instrumental in live cell imaging of telomeres in Nicotiana benthamiana. This method will further expand the CRISPR imaging toolkit, facilitating a better understanding of genome organization. Furthermore, we report the successful integration of the highly sensitive tyramide signal amplification method with CRISPR-FISH, demonstrating effective labeling of Arabidopsis centromeres.</p>","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11522987/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141897612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fengbo Yang, Tianyu Huang, Hong Tong, Xiaobin Shi, Rong Zhang, Weina Gu, Yue Li, Peng Han, Xiaoming Zhang, Yuting Yang, Zhixiong Zhou, Qingjun Wu, Youjun Zhang, Qi Su
Plant viruses exist in a broader ecological community that includes non-vector herbivores that can impact vector abundance, behavior, and virus transmission within shared host plants. However, little is known about the effects of non-vector herbivore infestation on virus transmission by vector insects on neighboring plants through inter-plant airborne chemicals. In this study, we investigated how volatiles emitted from tomato plants infested with the two-spotted spider mite (Tetranychus urticae) affect the infection of neighboring plants by tomato yellow leaf curl virus (TYLCV) transmitted by whitefly (Bemisia tabaci). Exposure of neighboring tomato plants to volatiles released from T. urticae-infested tomato plants reduced subsequent herbivory as well as TYLCV transmission and infection, and the jasmonic acid signaling pathway was essential for generation of the inter-plant defense signals. We also demonstrated that (E)-β-ocimene and methyl salicylic acid were two volatiles induced by T. urticae that synergistically attenuated TYLCV transmission and infection in tomato. Thus, our findings suggest that plant-plant communication via volatiles likely represents a widespread defensive mechanism that substantially contributes to plant fitness. Understanding such phenomena may help us to predict the occurrence and epidemics of multiple herbivores and viruses in agroecosystems, and ultimately to manage pest and virus outbreaks.
{"title":"Herbivore-induced volatiles reduce the susceptibility of neighboring tomato plants to transmission of a whitefly-borne begomovirus.","authors":"Fengbo Yang, Tianyu Huang, Hong Tong, Xiaobin Shi, Rong Zhang, Weina Gu, Yue Li, Peng Han, Xiaoming Zhang, Yuting Yang, Zhixiong Zhou, Qingjun Wu, Youjun Zhang, Qi Su","doi":"10.1093/jxb/erae342","DOIUrl":"10.1093/jxb/erae342","url":null,"abstract":"<p><p>Plant viruses exist in a broader ecological community that includes non-vector herbivores that can impact vector abundance, behavior, and virus transmission within shared host plants. However, little is known about the effects of non-vector herbivore infestation on virus transmission by vector insects on neighboring plants through inter-plant airborne chemicals. In this study, we investigated how volatiles emitted from tomato plants infested with the two-spotted spider mite (Tetranychus urticae) affect the infection of neighboring plants by tomato yellow leaf curl virus (TYLCV) transmitted by whitefly (Bemisia tabaci). Exposure of neighboring tomato plants to volatiles released from T. urticae-infested tomato plants reduced subsequent herbivory as well as TYLCV transmission and infection, and the jasmonic acid signaling pathway was essential for generation of the inter-plant defense signals. We also demonstrated that (E)-β-ocimene and methyl salicylic acid were two volatiles induced by T. urticae that synergistically attenuated TYLCV transmission and infection in tomato. Thus, our findings suggest that plant-plant communication via volatiles likely represents a widespread defensive mechanism that substantially contributes to plant fitness. Understanding such phenomena may help us to predict the occurrence and epidemics of multiple herbivores and viruses in agroecosystems, and ultimately to manage pest and virus outbreaks.</p>","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":null,"pages":null},"PeriodicalIF":5.6,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141912915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}