Anticancer effects of Artemisia campestris extract on acute myeloid leukemia cells: an ex vivo study.

IF 2.8 4区医学 Q2 ONCOLOGY Medical Oncology Pub Date : 2024-07-22 DOI:10.1007/s12032-024-02453-y

Rachid Kharrat, Fatma Ben Lakhal, Hiba Souia, Ines Limam, Hend Ben Naji, Mohamed Abdelkarim

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Abstract

Cure rates for acute myeloid leukemia (AML) remain suboptimal; thus, new treatment strategies are needed for this deadly disease. Artemisia campestris leaves hold significant value in traditional medicine. Despite extensive research conducted on this plant globally, the specific anti-AML properties of the leaves have received limited investigation. This study aims to explore the potential anti-leukemic activities of the ethyl acetate extract derived from Artemisia campestris (EAEAC), using mononuclear cells from bone marrow of thirteen AML patients. To this end, cytotoxic effects were evaluated using the MTT assay, and the mechanisms of cell death were investigated through various methods, including propidium iodide staining, annexin V/propidium iodide double staining, mitochondrial depolarization, and caspase-3/7 activation assays. Results demonstrated that EAEAC induced cell apoptosis by increasing DNA fragmentation, causing mitochondrial depolarization, and activating caspases 3/7. On the other hand, we assessed EAEAC's effect on two leukemia stem cell subpopulations, with results suggesting a potential decrease in their frequencies (three/five patients).

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青蒿提取物对急性髓性白血病细胞的抗癌作用：一项体外研究。

急性髓性白血病（AML）的治愈率仍然不理想；因此，这种致命疾病需要新的治疗策略。青蒿叶在传统医学中具有重要价值。尽管全球范围内对这种植物进行了广泛的研究，但对其叶片抗 AML 特性的调查却十分有限。本研究旨在利用 13 名急性髓细胞性白血病患者骨髓中的单核细胞，探索青蒿乙酸乙酯提取物（EAEAC）的潜在抗白血病活性。为此，采用 MTT 法评估了细胞毒性作用，并通过多种方法研究了细胞死亡机制，包括碘化丙啶染色法、附件素 V/ 碘化丙啶双重染色法、线粒体去极化法和 caspase-3/7 活化法。结果表明，EAEAC通过增加DNA碎片、引起线粒体去极化和激活caspase 3/7来诱导细胞凋亡。另一方面，我们评估了EAEAC对两种白血病干细胞亚群的影响，结果表明它们的频率可能会降低（3/5例患者）。

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来源期刊

Medical Oncology 医学-肿瘤学

CiteScore

4.20

自引率

2.90%

发文量

259

审稿时长

1.4 months

期刊介绍： Medical Oncology (MO) communicates the results of clinical and experimental research in oncology and hematology, particularly experimental therapeutics within the fields of immunotherapy and chemotherapy. It also provides state-of-the-art reviews on clinical and experimental therapies. Topics covered include immunobiology, pathogenesis, and treatment of malignant tumors.

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