The Bifidobacterium-dominated fecal microbiome in dairy calves shapes the characteristic growth phenotype of host.

IF 7.8 1区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY npj Biofilms and Microbiomes Pub Date : 2024-07-21 DOI:10.1038/s41522-024-00534-4
Yimin Zhuang, Shuai Liu, Duo Gao, Yiming Xu, Wen Jiang, Tianyu Chen, Jianxin Xiao, Jingjun Wang, Guobin Hou, Sumin Li, Xinjie Zhao, Yanting Huang, Shangru Li, Siyuan Zhang, Mengmeng Li, Wei Wang, Shengli Li, Zhijun Cao
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Abstract

The dominant bacteria in the hindgut of calves play an important role in their growth and health, which could even lead to lifelong consequences. However, the identification of core probiotics in the hindgut and its mechanism regulating host growth remain unclear. Here, a total of 1045 fecal samples were analyzed by 16S rRNA gene sequencing from the 408 Holstein dairy calves at the age of 0, 14, 28, 42, 56, and 70 days to characterize the dynamic changes of core taxa. Moreover, the mechanisms of nutrient metabolism of calf growth regulated by core bacteria were investigated using multi-omics analyses. Finally, fecal microbiota transplantation (FMT) in mice were conducted to illustrate the potential beneficial effects of core bacteria. Four calf enterotypes were identified and enterotypes dominated by Bifidobacterium and Oscillospiraceae_UCG-005 were representative. The frequency of enterotype conversion shifted from variable to stable. The close relationship observed between phenotype and enterotype, revealing a potential pro-growth effect of Bifidobacterium, might be implemented by promoting the use of carbohydrate, activating the synthesis of volatile fatty acids, amino acids and vitamin B6, and inhibiting methane production in the hindgut. The FMT results indicated the beneficial effect of Bifidobacterium on host growth and hindgut development. These results support the notion that the Bifidobacterium-dominated fecal microbiome would be an important driving force for promoting the host growth in the early life. Our findings provide new insights into the potential probiotic mining and application strategies to promote the growth of young animals or improve their growth retardation.

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以双歧杆菌为主的乳牛粪便微生物群塑造了宿主的生长表型特征。
犊牛后肠中的优势菌对其生长和健康起着重要作用,甚至可能导致终身后果。然而,后肠中核心益生菌的鉴定及其调控宿主生长的机制仍不清楚。本文通过 16S rRNA 基因测序分析了 408 头荷斯坦奶牛在 0、14、28、42、56 和 70 日龄时的 1045 份粪便样本,以描述核心类群的动态变化。此外,还利用多组学分析方法研究了核心菌调控犊牛生长的营养代谢机制。最后,对小鼠进行了粪便微生物群移植(FMT),以说明核心菌的潜在有益作用。研究发现了四种小牛肠型,其中以双歧杆菌和Oscillospiraceae_UCG-005为主的肠型具有代表性。肠型转换的频率从可变到稳定。观察到的表型与肠型之间的密切关系揭示了双歧杆菌潜在的促生长效应,可能是通过促进碳水化合物的利用,激活挥发性脂肪酸、氨基酸和维生素 B6 的合成,以及抑制后肠甲烷的产生来实现的。FMT 结果表明,双歧杆菌对宿主的生长和后肠发育有益。这些结果支持了一种观点,即双歧杆菌主导的粪便微生物组将成为促进宿主早期生长的重要动力。我们的发现为潜在益生菌的挖掘和应用策略提供了新的见解,以促进幼年动物的生长或改善其生长迟缓。
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来源期刊
npj Biofilms and Microbiomes
npj Biofilms and Microbiomes Immunology and Microbiology-Microbiology
CiteScore
12.10
自引率
3.30%
发文量
91
审稿时长
9 weeks
期刊介绍: npj Biofilms and Microbiomes is a comprehensive platform that promotes research on biofilms and microbiomes across various scientific disciplines. The journal facilitates cross-disciplinary discussions to enhance our understanding of the biology, ecology, and communal functions of biofilms, populations, and communities. It also focuses on applications in the medical, environmental, and engineering domains. The scope of the journal encompasses all aspects of the field, ranging from cell-cell communication and single cell interactions to the microbiomes of humans, animals, plants, and natural and built environments. The journal also welcomes research on the virome, phageome, mycome, and fungome. It publishes both applied science and theoretical work. As an open access and interdisciplinary journal, its primary goal is to publish significant scientific advancements in microbial biofilms and microbiomes. The journal enables discussions that span multiple disciplines and contributes to our understanding of the social behavior of microbial biofilm populations and communities, and their impact on life, human health, and the environment.
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